Article
Cell Biology
Juan Ge, Congyang Li, Hongzheng Sun, Yongan Xin, Shuai Zhu, Yuan Liu, Shoubin Tang, Longsen Han, Zhenyue Huang, Qiang Wang
Summary: Maternal obesity leads to telomere shortening and defective telomeres in oocytes and early embryos, which in turn affects the development of embryos.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Lucy May Vining, Louisa J. Zak, Simon Crawford Harvey, Katie Evelyn Harvey
Summary: Cryopreservation of gametes and embryos is crucial for assisted reproductive technology and livestock reproduction. Freezing procedures may induce cell apoptosis, and inhibiting apoptosis could improve cryosurvival.
Article
Reproductive Biology
Pierre Comizzoli, Olga Amelkina, Daniela R. Chavez, Tricia R. Rowlison, Pei-Chih Lee
Summary: In addition to companion animals and laboratory species, approximately 270 carnivore species play vital ecological roles in various ecosystems. However, the majority of these species are now threatened or endangered in the wild due to human activities. Protecting their natural habitats is crucial, but understanding carnivore reproduction, particularly in terms of sperm, oocyte, and embryo biology, is equally important for maintaining sustainable populations and conservation efforts. This review aims to provide the latest knowledge on the biology of sperm cells, oocytes, and early embryos across all carnivore families, highlighting the need for further research and the development of conservation strategies.
Review
Obstetrics & Gynecology
Brid Ni Dhonnabhain, Nagla Elfaki, Kyra Fraser, Aviva Petrie, Benjamin P. Jones, Srdjan Saso, Paul J. Hardiman, Natalie Getreu
Summary: This study compares obstetric outcomes in cancer patients who undergo cryopreservation of reproductive cells or tissues before gonadotoxic therapy. The results indicate that cryopreservation of oocytes, embryos, and ovarian tissue has similar effectiveness in terms of clinical pregnancy and live birth rates. However, ovarian tissue cryopreservation is associated with a significantly lower miscarriage rate compared to embryo cryopreservation.
FERTILITY AND STERILITY
(2022)
Review
Andrology
George Anifandis, Christina I. Messini, Mara Simopoulou, George Sveronis, Antonios Garas, Alexandros Daponte, Ioannis E. Messinis
Summary: The COVID-19 pandemic has presented unprecedented global challenges, leading to various measures being implemented by different countries to combat the spread of the virus. The virus may have potential effects on male fertility and IVF treatments, as well as the cryopreservation procedures of gametes and embryos which need to be virus-free to avoid contamination.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2021)
Review
Reproductive Biology
Betul Tire, Saffet Ozturk
Summary: Telomeres are repetitive DNA sequences that protect the ends of eukaryotic chromosomes and play a crucial role in maintaining the integrity and stability of the genome. Shortening of telomeres can occur due to factors such as aging, DNA replication, oxidative stress, and genotoxic agents. However, telomeres can be lengthened by telomerase and alternative lengthening mechanisms. If telomeres become critically short, it can lead to genomic instability and abnormalities in cell division. The effects of assisted reproductive technologies (ARTs), such as ovarian stimulation, culture conditions, and cryopreservation procedures, on telomere length and telomerase activity in oocytes and embryos have been extensively studied. These parameters could potentially serve as biomarkers for assessing the quality of oocytes and embryos in ART centers.
JOURNAL OF OVARIAN RESEARCH
(2023)
Review
Obstetrics & Gynecology
Hisataka Iwata
Summary: Resveratrol has been found to enhance mitochondrial biogenesis and degradation, aiding in the recovery of mitochondrial function and improving embryo development in cryopreservation.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Irene Peinado, Isabel Moya, Paula Saez-Espinosa, Macarena Barrera, Laura Garcia-Valverde, Raquel Frances, Patricia Torres, Maria Jose Gomez-Torres
Summary: Vitrification of immature oocytes followed by maturation showed similar rates of survival, maturation, and metaphase plate conformation compared to the standard strategy, but with a significantly higher percentage of normal spindle. Additionally, extending the IVM time to 48 hours did not seem to negatively affect the oocyte metaphase plate configuration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Mary Hagedorn, Christopher A. Page, Keri L. O'Neil, Daisy M. Flores, Lucas Tichy, Trinity Conn, Valerie F. Chamberland, Claire Lager, Nikolas Zuchowicz, Kathryn Lohr, Harvey Blackburn, Tali Vardi, Jennifer Moore, Tom Moore, Iliana B. Baums, Mark J. A. Vermeij, Kristen L. Marhaver
Summary: The study demonstrated the successful use of cryopreserved sperm for assisted gene flow in corals, showing it to be a viable conservation tool to increase genetic diversity in threatened marine populations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Jose Carlos Ferreira-Silva, Roberta Lane Oliveira Silva, Joane Isis Travassos Vieira, Jessica Barboza Silva, Lethicia Souza Tavares, Fabiana Aparecida Cavalcante Silva, Elton Pedro Nunes Pena, Maiana Silva Chaves, Marcelo Tigre Moura, Tercilio Calsa Junior, Ana Maria Benko-Iseppon, Vicente Jose Figueiredo Freitas, Marcos Antonio Lemos Oliveira
Summary: Cryopreservation negatively impacts the morphology and viability of in vitro-produced goat embryos, leading to higher expression levels of CPT2 and HSP90 genes in cryopreserved embryos compared to in vivo counterparts. No significant difference was observed in the transcription level of SOD gene between the groups. The conservation of CPT2 and HSP90 proteins among mammals suggests they share similar functions.
Article
Biochemistry & Molecular Biology
Pauliina Paloviita, Christel Hyden-Granskog, Dawit A. Yohannes, Priit Paluoja, Juha Kere, Juha S. Tapanainen, Kaarel Krjutskov, Timo Tuuri, Urmo Vosa, Sanna Vuoristo
Summary: This study analyzed sRNAs in human oocytes and early embryos, revealing the dynamics of sRNA expression during OET. In humans, OET is temporally coupled with the transition from os-piRNAs to miRNAs, and miRNA modification is markedly increased in zygotes.
Review
Obstetrics & Gynecology
Alan Penzias, Kristin Bendikson, Tommaso Falcone, Karl Hansen, Micah Hill, William Hurd, Sangita Jindal, Suleena Kalra, Jennifer Mersereau, Catherine Racowsky, Robert Rebar, Richard Reindollar, Anne Steiner, Dale Stovall, Cigdem Tanrikut, Sangita Jindal, Kathryn Go, James Stachecki, Zsolt Peter Nagy
Summary: This paper reviews best practices for rapid-cooling cryopreservation of oocytes and embryos, including the types of cryoprotectants and cryo devices typically used, as well as defining key performance indicators of rapid-cooling vitrification success.
FERTILITY AND STERILITY
(2021)
Article
Medicine, Research & Experimental
Jianhua Li, Jing Chen, Tiecheng Sun, Shuiwen Zhang, Tingting Jiao, Ri-Cheng Chian, Youzhu Li, Ye Xu
Summary: In vitro oocyte maturation (IVM) does not affect the quality of embryos and the aneuploidy rate of embryos. The aneuploidy rate of embryos increases with maternal age, especially over 35 years old, whether in IVF or IVM group. Embryos with poor quality, especially grade III embryos, are recommended to be tested by PGS.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Gilad Karavani, Peera Wasserzug-Pash, Talya Mordechai-Daniel, Dvora Bauman, Michael Klutstein, Tal Imbar
Summary: The study evaluated in vitro maturation (IVM) outcomes in 133 chemotherapy naive patients aged 1-35 years. The results showed a gradual increase in IVM rates from 1 to 24 years, with a peak in the 18-24 years group and a decrease in the 25-35 years group. Significantly lower success rates were observed in patients under 6 years old and over 30 years old compared to the 18-24 years group.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Obstetrics & Gynecology
Tianqi Cao, Jing Guo, Yan Xu, Xiufeng Lin, Weifen Deng, Lizi Cheng, Huan Zhao, Shan Jiang, Min Gao, Junjiu Huang, Yanwen Xu
Summary: Two TUBB8 mutations (p.A54V and p.R320H) were identified in female infertility patients, with functional assays revealing distinct effects on cell structures and reproductive cell development. The study highlights the importance of in-vitro functional assays in confirming the pathogenicity of genetic mutations associated with primary female infertility.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
