Article
Multidisciplinary Sciences
Yancong Zhang, Amrisha Bhosle, Sena Bae, Lauren J. McIver, Gleb Pishchany, Emma K. Accorsi, Kelsey N. Thompson, Cesar Arze, Ya Wang, Ayshwarya Subramanian, Sean M. Kearney, April Pawluk, Damian R. Plichta, Ali Rahnavard, Afrah Shafquat, Ramnik J. Xavier, Hera Vlamakis, Wendy S. Garrett, Andy Krueger, Curtis Huttenhower, Eric A. Franzosa
Summary: This article investigates the role of microbial communities and their associated bioactive compounds in inflammatory bowel diseases (IBD). It systematically identifies potentially bioactive protein families involved in gut inflammation during IBD and validates their bioactivity using a combination of metagenomics, metatranscriptomics, and metaproteomics. The results provide candidate microbial proteins that interact with the host immune system in IBD, expanding our understanding of potentially bioactive gene products in chronic diseases.
Article
Microbiology
Zhen -Ping Zou, Yue Du, Ting -Ting Fang, Ying Zhou, Bang -Ce Ye
Summary: Engineered probiotic i-ROBOT has been developed to noninvasively monitor and record the occurrence and progression of inflammatory bowel disease (IBD) in real time, and release treatments based on disease-associated biomarkers. It provides a heritable genomic DNA sequence and a colorimetric signal by activating a base-editing system in response to the inflammatory marker thiosulfate. In experiments with mice, i-ROBOT effectively ameliorated colitis and showed potential for the treatment of gastrointestinal and metabolic disorders.
CELL HOST & MICROBE
(2023)
Article
Immunology
Milad Abdi, Vahid Lohrasbi, Arezoo Asadi, Maryam Esghaei, Faramarz Masjedian Jazi, Mahdi Rohani, Malihe Talebi
Summary: The lactobacilli strains isolated from breast milk showed promising probiotic properties, including presence of specific bacteriocin genes, strong cell adhesion ability, competition with intestinal pathogens, cholesterol-lowering effects, and improvement in inflammatory bowel disease. Further studies could potentially lead to their consideration as probiotic strains.
MICROBIAL PATHOGENESIS
(2021)
Article
Food Science & Technology
Cuijiao Feng, Weiqin Zhang, Tao Zhang, Bohai Li, Qiuwen He, Lai-Yu Kwok, Heping Zhang
Summary: This study found that oral administration of pasteurized probiotic fermented milk can significantly alleviate IBD symptoms, reduce inflammation, and restore the stability of the gut microbiota.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Review
Biochemistry & Molecular Biology
Zejing Zhao, Shumin Xu, Wangyang Zhang, Danjun Wu, Gensheng Yang
Summary: This review discusses the probiotic properties of Escherichia coli NISSLE 1917 (EcN) in the treatment of inflammatory bowel disease (IBD), particularly ulcerative colitis. The mechanisms of EcN in the remission of IBD are proposed, and recent advancements on functionalized EcN are compiled to provide novel therapeutic strategies for the prevention and treatment of IBD.
Review
Food Science & Technology
Jeongmin Cha, Yeon Bee Kim, Seong-Eun Park, Se Hee Lee, Seong Woon Roh, Hong-Seok Son, Tae Woong Whon
Summary: Kimchi is a traditional fermented vegetable side dish in Korea and has become a global health food. It undergoes spontaneous fermentation, mainly by lactic acid bacteria (LAB) originating from its raw ingredients. The LAB present in kimchi have numerous health benefits and probiotic potential, making kimchi a potential probiotic food.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Food Science & Technology
Xiaolin Liu, Xuepeng Lv, Yue Sun, Chunhong Liu, Rongchun Wang, Rongmei Liu, Ying Ma, Qiming Li
Summary: The probiotic characteristics of Lacticaseibacillus rhamnosus grx10 were investigated through genotype and phenotype analysis in this study. In vitro experiments showed that grx10 exhibited excellent resistance to ambient stresses, bacteriostatic activity, and adhesion ability, indicating its potential as a probiotic. Complete genome sequencing revealed the genetic information and genes associated with probiotic characteristics, as well as the prediction of genes involved in extracellular proteins and secretory pathways. Among the secreted proteins, p40 and p75 were found to promote intestinal barrier integrity, with an abundance of 0.36% and 0.20% in the fermented supernatant of grx10. This study highlights the potential of grx10 in protecting the intestinal barrier from a postbiotic perspective.
Article
Multidisciplinary Sciences
Roya Daneshazari, Mohammad Rabbani Khorasgani, Afrouzossadat Hosseini-Abari, June-Hyung Kim
Summary: In this study, two strains of Bacillus isolated from camel milk were evaluated and exhibited characteristics of potential probiotics, including acid and bile salts resistance, surface hydrophobicity, auto-aggregation, antioxidant characteristics, and adherence to intestinal cells. These results suggest that Bacillus CM1 and CM2 strains have the potential to be considered as probiotics.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Faezeh Salek, Hamid Mirzaei, Jalil Khandaghi, Afshin Javadi, Yousef Nami
Summary: The potential probiotic Enterococcus faecalis KUMS-T48, isolated from an Iranian traditional dairy product, has been found to have anti-pathogenic, anti-inflammatory, and anti-proliferative properties against specific cancer cell lines. The strain showed strong effects against Bacillus subtilis and Listeria monocytogenes, moderate effect against Yersinia enterocolitica, and weak effects against Klebsiella pneumoniae and Escherichia coli. Additionally, the cell-free supernatant of E. faecalis KUMS-T48 inhibited the proliferation of cancer cells in a similar manner to Taxol, but without affecting normal cell lines. The supernatant was found to have a proteinaceous nature and induced apoptosis-based cytotoxic mechanism through the regulation of specific genes.
SCIENTIFIC REPORTS
(2023)
Review
Cell Biology
Jayshree Mishra, Madyson Stubbs, Longxiang Kuang, Nitza Vara, Priyam Kumar, Narendra Kumar
Summary: Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract. Probiotics and their engineering show great potential in treating chronic inflammatory conditions, including IBD. Personalized probiotic treatment is important due to the diversity of the gut microbiome in different populations.
MEDIATORS OF INFLAMMATION
(2022)
Article
Multidisciplinary Sciences
Jun Liu, Yixin Wang, William John Heelan, Yu Chen, Zhaoting Li, Quanyin Hu
Summary: Inflammatory bowel diseases (IBDs) are associated with elevated levels of reactive oxygen species (ROS) and dysregulated gut microbiota. In this study, researchers developed ROS-scavenging nanoparticles (HPN) to effectively scavenge ROS. They targeted the colon tissue by conjugating HPN to modified probiotic Escherichia coli Nissle 1917 (EcN). To enhance the efficacy of bacteriotherapy, the EcN cells were encapsulated with a poly-norepinephrine (NE) layer for protection against environmental assaults and prolonged retention in the intestine. The HPN-NE-EcN treatment showed enhanced efficacy in colitis models and increased abundance and diversity of gut microbiota.
Review
Biochemistry & Molecular Biology
Shaghayegh Baradaran Ghavami, Hamid Asadzadeh Aghdaei, Dario Sorrentino, Shabnam Shahrokh, Maryam Farmani, Fatemeh Ashrafian, Maria Pina Dore, Shahrbanoo Keshavarz Azizi Raftar, Seyed Mobin Khoramjoo, Mohammad Reza Zali
Summary: Inflammatory bowel diseases (IBDs) are immune-mediated, chronic relapsing diseases with a rising prevalence worldwide. Probiotic bacteria may impact immunomodulation by regulating dendritic cells to produce tolerogenic DCs, which could play a role in the treatment of IBD and associated conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Carine N. Almada-Erix, Caroline N. Almada, Geany T. Souza Pedrosa, Joao Paulo Biachi, Mariane S. Bonatto, Marcio Schmiele, Elizabeth H. Nabeshima, Maria Teresa P. S. Clerici, Marciane Magnani, Anderson S. Sant'Ana
Summary: This study evaluated the resistance of Bacillus coagulans spores in bread processing and storage, and characterized the physicochemical parameters of probiotic bread. Results showed that baking had the highest impact on the survival of Bacillus coagulans, and both white and whole wheat bread were suitable carriers for delivering the probiotic.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Ying Qi, Sheng-qi Zang, Juan Wei, Hong-chuan Yu, Zhao Yang, Hui-min Wu, Ying Kang, Hui Tao, Miao-fang Yang, Lei Jin, Ke Zen, Fang-Yu Wang
Summary: The study evaluated variations in salivary microbiota in IBD patients and healthy controls, finding dysbiosis in IBD characterized by changes in microbiota biodiversity and composition, and significant increases in certain oral biofilm-forming bacteria. Levels of inflammatory cytokines associated with IBD were also found to be elevated and correlated with specific bacteria, with functional variations identified in genetic information processing, carbohydrate metabolism, and protein processing in endoplasmic reticulum in IBD.
Article
Chemistry, Multidisciplinary
Kairuo Wang, Qian Chen, Li Ding, Yefei Zhu, Xinyue Wang, Mengting Zhou, Meiqi Chang, Manman Pei, Yuanyuan Zhang, Yang Zhang, Yu Chen, Huanlong Qin
Summary: This study developed a mucoadhesive probiotic-mediated therapeutic strategy to achieve bioadhesion and prolong the residence time of oral medication in the intestinal tract. It effectively modulated intestinal inflammation and microbiota, enhancing the therapeutic effect.
Article
Biochemistry & Molecular Biology
Roberto Gozalbo-Rovira, Antonio Rubio-del-Campo, Cristina Santiso-Bellon, Susana Vila-Vicent, Javier Buesa, Susana Delgado, Natalia Molinero, Abelardo Margolles, Maria Jesus Yebra, Maria Carmen Collado, Vicente Monedero, Jesus Rodriguez-Diaz
Summary: The study identified Ruminococcus and Oxalobacter as important bacteria in interacting with rotavirus during infection in children with diarrhea. Specific bacteria like Ruminococcus may negatively affect rotavirus infection by reducing infectivity. This suggests a pivotal interaction between certain bacterial taxa in the intestinal microbiota and human rotavirus, pointing towards new possibilities for antiviral strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Raquel Marcos-Fernandez, Lorena Ruiz, Aitor Blanco-Miguez, Abelardo Margolles, Borja Sanchez
Summary: This study describes a new procedure for targeted modification of the human gut microbiota using antibodies against specific bacterial surface-associated proteins. By conjugating antibodies with fluorescent probes and magnetic particles, specific identification and depletion of a bacterium in the microbiota was achieved. This method offers new ways to study and treat specific diseases by manipulating key bacteria in the gut microbiota.
SCIENTIFIC REPORTS
(2021)
Review
Pharmacology & Pharmacy
Lorena del Pozo-Acebo, Maria-Carmen Lopez de las Hazas, Abelardo Margolles, Alberto Davalos, Almudena Garcia-Ruiz
Summary: Cross-kingdom communication through non-coding RNAs, particularly exogenous miRNAs ingested through diet, has risen as a novel discovery. These miRNAs, considered unstable in the gastrointestinal tract, may resist conditions and exert regulatory effects after being transported in extracellular vesicles. Despite challenges, extracellular miRNAs show promise as potential therapeutic agents for future pharmacological development.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Chemistry, Applied
Carlos Sabater, Ana Blanco-Doval, Abelardo Margolles, Nieves Corzo, Antonia Montilla
Summary: This study aimed to develop a comprehensive workflow to elucidate molecular features of artichoke pectic oligosaccharides (POS) contributing to high potential prebiotic activity. By optimizing the obtainment of artichoke POS and characterizing the oligosaccharide structures, the study proposed interaction mechanisms between POS and microbial glycosidases, as well as integrated chemical information in virtual simulations of colonic fermentation. The established structure-activity relationships could potentially help in the rational design of prebiotics and in clinical trials.
CARBOHYDRATE POLYMERS
(2021)
Article
Microbiology
Gabriele Andrea Lugli, Giulia Alessandri, Christian Milani, Alice Viappiani, Federico Fontana, Chiara Tarracchini, Leonardo Mancabelli, Chiara Argentini, Lorena Ruiz, Abelardo Margolles, Douwe van Sinderen, Francesca Turroni, Marco Ventura
Summary: Utilizing in silico data obtained from metabolic modeling to design a chemically defined medium (CDM), this study successfully isolated and sequenced genomes of six putative novel bacterial species from the gut of non-human primates.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Engineering, Electrical & Electronic
Mabel Ponton, Sergio Sancho, Amparo Herrera, Almudena Suarez
Summary: The study examines the application of Zero-IF self-oscillating mixers (SOM), analyzing the potential of a system composed of two Zero-IF SOMs for short-range communications and proposing design criteria.
IEEE TRANSACTIONS ON MICROWAVE THEORY AND TECHNIQUES
(2022)
Article
Biochemical Research Methods
Guillermo Blanco, Borja Sanchez, Lorena Ruiz, Florentino Fdez-Riverola, Abelardo Margolles, Analia Lourenco
Summary: This study introduces a new method to predict glycolytic abilities in sequenced genomes, helping to target specific carbohydrates and identify potential sources of specialised enzymes. By analyzing bacterial families, the method successfully predicted the potential presence of glycoside hydrolases in a large number of species.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2021)
Review
Biotechnology & Applied Microbiology
Felipe Gonzalez-Gonzalez, Susana Delgado, Lorena Ruiz, Abelardo Margolles, Patricia Ruas-Madiedo
Summary: Fermentation has traditionally been used for food preservation, but modern industrial processes aim to improve the functional characteristics of the final product. Starter cultures, adjunct cultures, and probiotics play essential roles in achieving high-quality foods with improved nutritional and health-promoting properties. This review focuses on the functional characteristics of these cultures, including their synthesis of metabolites for preservation and safety, their impact on organoleptic properties, and their contributions to nutrition and health improvement.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Review
Microbiology
Raquel Marcos-Fernandez, Borja Sanchez, Lorena Ruiz, Abelardo Margolles
Summary: Flow cytometry is a powerful tool for analyzing cell populations, and in recent years, it has emerged as an important technique in microbiology for studying microbes and their interactions with host cells.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Carlos Sabater, Ines Calvete-Torre, Lorena Ruiz, Abelardo Margolles
Summary: This study used metagenomics to investigate the differences in carbohydrate metabolic capabilities between healthy individuals and those with inflammatory bowel disease (CD). The findings suggest that specific prebiotic interventions, such as arabinoxylan and pectin, could rebalance dysbiosis in CD by promoting the growth of key metabolic species and facilitating metabolic interactions with other beneficial microbes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Natalia Molinero, Elena Conti, Alan W. Walker, Abelardo Margolles, Sylvia H. Duncan, Susana Delgado
Summary: This study presents unique insights into metabolic interactions between two bacterial isolates, Ruminococcus gauvreauii IPLA60001 and Ruminococcoides bili IPLA60002, from human gallbladder. The strains showed resistance to bile and differed in carbohydrate metabolism, amino acid catabolism, and vitamin synthesis capabilities. Transcriptomic analysis revealed their synergistic interactions, providing evidence for syntrophic metabolic cooperation between bacterial strains isolated from human bile.
MICROBIOLOGY SPECTRUM
(2022)
Article
Nutrition & Dietetics
Claudia Barber, Carlos Sabater, Maria Angeles Avila-Galvez, Fernando Vallejo, Rogger Alvaro Bendezu, Laetitia Guerin-Deremaux, Francisco Guarner, Juan Carlos Espin, Abelardo Margolles, Fernando Azpiroz
Summary: Resistant dextrin affects gut microbiota metabolism and composition, reducing gas production and providing health benefits in blood glucose management and satiety.
Article
Microbiology
Raquel Marcos-Fernandez, Sabino Riestra, Rebeca Alonso-Arias, Lorena Ruiz, Borja Sanchez, Abelardo Margolles
Summary: This study developed a strategy to modify the relative abundance of F. prausnitzii in fecal microbiotas and assessed its contribution to the immunomodulatory effect of intestinal microbiotas using a PBMC model. The results showed that reduction in the abundance of F. prausnitzii is related to several human disorders.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Pilar Lumbreras-Iglesias, Carlos Sabater, Ainhoa Fernandez Moreno, Paula Lopez de Ugarriza, Ana Fernandez-Verdugo, Abelardo Margolles, Maria Rosario Rodicio, Teresa Bernal, Javier Fernandez
Summary: Patients diagnosed with acute leukemia often have a weakened immune system, making infections a major concern. This study used shotgun metagenomics to screen the gut of recovered leukemia patients for ESBL- and/or carbapenemase-producing Enterobacterales. The results showed no presence of these bacteria, indicating successful decolonization and positive implications for future clinical management. Further studies are needed to validate the utility of metagenomics in routine screening of multidrug-resistant bacteria in onco-hematological patients.
Article
Food Science & Technology
Ines Calvete-Torre, Carlos Sabater, Susana Delgado, Patricia Ruas-Madiedo, Alicia Ruperez-Garcia, Antonia Montilla, F. Javier Moreno, Abelardo Margolles, Lorena Ruiz
Summary: Arabinoxylan (AX) and arabinoxylo-oligosaccharides (AXOS) are prebiotics that promote beneficial species in the human gut, such as bifidobacteria. Bifidobacterium longum and B. longum subsp. longum have the highest capability to metabolize complex AX substrates, while B. longum subsp. infantis also possesses some machinery related to AXOS metabolization. A differential carbohydrate free medium (CFM) supplemented with AX and AXOS was developed to selectively isolate novel probiotic bifidobacteria that can metabolize structurally complex AX-derived prebiotics.
FOOD RESEARCH INTERNATIONAL
(2023)
