Article
Cell & Tissue Engineering
Z. Zong, X. Zhang, Z. Yang, W. Yuan, J. Huang, W. Lin, T. Chen, J. Yu, J. Chen, L. Cui, G. Li, B. Wei, S. Lin
Summary: This study demonstrated that a stepwise preconditioning approach significantly enhanced the proliferation and chondrogenic potential of ageing hMSCs, and also reduced immunogenicity and senescence. Animal studies showed that the therapeutic effect of preconditioned MSCs in preventing OA development depended on the number of cells applied, with the best effect observed when treated with eight millions of hM-MSCs.
BONE & JOINT RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Ga Yeon Kim, Gyu Tae Choi, Jinryong Park, Jeongeun Lee, Jeong Tae Do
Summary: In this study, two types of porcine MSCs were compared, and it was found that adipose-derived stem cells showed better proliferation capacity, adipogenic and chondrogenic differentiation potential, and mitochondrial metabolism compared to Wharton's jelly-derived mesenchymal stem cells. This study provides insights into the characteristics of porcine MSCs and their potential applications in animal clinics and cultured meat science.
Article
Biochemistry & Molecular Biology
Eun-Ju Lee, Khurshid Ahmad, Shiva Pathak, SunJu Lee, Mohammad Hassan Baig, Jee-Heon Jeong, Kyung-Oh Doh, Dong-Mok Lee, Inho Choi
Summary: This study introduces novel peptides derived from fibronectin that enhance cell adhesion, proliferation, and differentiation, offering a new approach to cell culture. The peptides showed significant effects on primary cells and stem cells, increasing proliferation and promoting differentiation into specific cell types such as osteoblasts. The results suggest potential applications in the production of core materials for cell culture such as scaffolds and culture medium components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Erika N. Guerrero, Shantal Vega, Cindy Fu, Ruth De Leon, Davis Beltran, Mairim Alexandra Solis
Summary: Maternal age influences the biological characteristics and stemness properties of PDMSCs, with cells from mothers aged 22-35 showing higher pluripotency and differentiation effectiveness, while donors aged 26-30 exhibit greater self-renewal ability.
Article
Agriculture, Dairy & Animal Science
Manuela Heimann, Mohamed I. Elashry, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The investigation of mesenchymal stem cells has become an interesting scientific field in regenerative medicine and organ transplantation. This study focused on establishing an adipogenic differentiation protocol suitable for different mammalian species, and found that using rosiglitazone instead of traditional protocols significantly improves cell viability and adipogenic differentiation capacity. The study also revealed differences between species in terms of fat formation and fat-regulating gene expression. This information is essential for selecting a stem cell specification program and improving clinical targeting of obesity and metabolic disorders in veterinary medicine.
Article
Biotechnology & Applied Microbiology
Hyoungki Kim, Soonjo Kwon
Summary: The study demonstrates that a hypoxic environment, created using specific chemicals in a suspension culture, enhances the proliferation of mouse clonal mesenchymal stem cells and promotes their differentiation into osteogenic cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Cell Biology
Gi Cheol Park, Ji Min Kim, Sung-Chan Shin, Yong-il Cheon, Eui-Suk Sung, Minhyung Lee, Jin-Choon Lee, Byung-Joo Lee
Summary: Human tonsil-derived mesenchymal stem cells (TMSCs) have a higher proliferation rate and differentiation potential compared to adipose-tissue-derived MSCs (AMSCs) or bone-marrow-derived MSCs (BMSCs). TMSCs exhibit a significantly higher expression of the tensin3 gene (TNS3) than AMSCs or BMSCs. TNS3 plays a vital role in regulating cell migration, gene and protein expression, and integrin activity in TMSCs.
Article
Biology
Rafal B. Lewandowski, Malgorzata Stepinska, Andrzej Gietka, Monika Dobrzynska, Mariusz P. Lapinski, Elzbieta A. Trafny
Summary: LED irradiation at a wavelength of 630 nm, energy density 4 J/cm(2), and power density 17 mW/cm(2) can effectively increase the number of viable hBM-MSCs in vitro while maintaining their crucial surface antigens and differentiation potential.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2021)
Review
Engineering, Biomedical
Jay R. K. Samal, Vignesh K. Rangasami, Sumanta Samanta, Oommen P. Varghese, Oommen P. Oommen
Summary: Studies have shown that culturing MSCs in a low oxygen environment can upregulate stem cell markers and promote cell proliferation in an undifferentiated state. However, there is ambiguity in the results due to differences in oxygen gradients and culture conditions among different studies. This progress report focuses on conflicting findings related to the application of hypoxic conditions for improving MSC proliferation or differentiation, aiming to decipher the factors influencing MSC characteristics under hypoxia.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Agriculture, Dairy & Animal Science
Ejaz R. Dar, Mudasir B. Gugjoo, Moien Javaid, Shahid Hussain, Mujeeb R. Fazili, Kuldeep Dhama, Taha Alqahtani, Ali M. Alqahtani, Riaz A. Shah, Talha Bin Emran
Summary: The study evaluates the influence of ambient temperature, donor physiological status, and tissue source on sheep mesenchymal stem cells. Results show that adipose tissue-derived MSCs from full term pregnant female sheep exhibit higher potential for proliferation and differentiation compared to male sheep-derived MSCs. Cryopreserved MSCs have similar characteristics to fresh cells. Therefore, it is important to consider tissue type and donor physiological status when applying MSCs in clinical settings.
Article
Neurosciences
Nader Asadian, Majid Jadidi, Manouchehr Safari, Taha Jadidi, Mahbobeh Gholami
Summary: The study revealed that sinusoidal 25 Hz, square 50 Hz, and sinusoidal 75 Hz EMF groups had negative effects on the viability and proliferation rate of bone marrow mesenchymal stem cells (BMMSC), while sinusoidal 50 Hz and 75 Hz groups promoted differentiation into neurons. Different frequencies of EMF had varying impacts on the differentiation into astrocyte cells. Further research on the effects of EMF with broader flux density and frequency on BMMSC is recommended.
NEUROSCIENCE LETTERS
(2021)
Review
Genetics & Heredity
Huarui Cai, Zhongze Wang, Wenhan Tang, Xiaoxue Ke, Erhu Zhao
Summary: This review discusses the role of mTOR in mesenchymal stem cells and its potential for treating ageing-related diseases.
FRONTIERS IN GENETICS
(2022)
Article
Cell & Tissue Engineering
Guanyin Chen, Dong Fan, Wangqian Zhang, Shuning Wang, Jintao Gu, Yuan Gao, Lei He, Weina Li, Cun Zhang, Meng Li, Yingqi Zhang, Zhaohui Liu, Qiang Hao
Summary: This study found that under hypoxic conditions, there was a significant increase in the expression of Mkx in MSCs. Mkx mediated hypoxia-induced tenogenic differentiation of MSCs but could not completely repress the proliferation of hypoxic MSCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Veterinary Sciences
Metka Voga, Valerija Kovac, Gregor Majdic
Summary: The study compared the characteristics of adipose-derived MSCs from dogs and domestic cats, revealing a higher percentage of CD44(+)CD90(+) and CD34(-/-) cells in canine MSCs. Additionally, canine ADMSCs demonstrated greater proliferation and differentiation potential compared to feline ADMSCs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Engineering, Biomedical
Leila Gholami, Seyedeh Sareh Hendi, Massoud Saidijam, Roghayeh Mahmoudi, Rana Tarzemany, Aliasghar Arkian, Saeid Afshar, Reza Fekrazad
Summary: The study evaluated the effect of near-infrared PBM on inflamed periodontal ligament stem cells, showing that PBM did not significantly increase cell proliferation but significantly increased the expression of osteogenic-related genes and alkaline phosphatase activity.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Matthew Anderson-Baron, Yan Liang, Melanie Kunze, Aillette Mulet-Sierra, Martin Osswald, Khalid Ansari, Hadi Seikaly, Adetola B. Adesida
Summary: The study demonstrated that the hormone PTHrP effectively suppressed chondrocyte hypertrophy marker COL10A1 in NC-MSC cocultures, leading to the formation of stable non-calcifying cartilage and preventing calcification and vascularization in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaoyi Lan, Yan Liang, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Tianxing Gong, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: Skin cancer patients with tumorigenic lesions on their noses often require surgical resection resulting in nasal cartilage removal. Autologous tissue-engineered nasal cartilage grafts can help mitigate the problem, despite the issue of inhomogeneous distribution of cartilage matrix. Advances in 3D bioprinting technology offer customizable and anatomically shaped cartilages without the distribution issues.
Article
Multidisciplinary Sciences
Alexander R. A. Szojka, Colleen N. Moore, Yan Liang, Stephen H. J. Andrews, Melanie Kunze, Aillette Mulet-Sierra, Nadr M. Jomha, Adetola B. Adesida
Summary: Low oxygen and mechanical loading may regulate the fibrocartilaginous phenotype of the human inner meniscus, but their combined effect in engineered tissues is not well-studied. The study found that neither hypoxia under static culture nor hypoxia combined with dynamic compression significantly affected the expression of specific markers for the fibrocartilaginous matrix-forming phenotype in human meniscus fibrochondrocytes. Additionally, mechanical properties increased over a two-week loading period regardless of oxygen tension and loading regime.
Article
Orthopedics
Alexander R. A. Szojka, Yan Liang, Rita de Cassia Marqueti, Colleen N. Moore, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Nadr M. Jomha, Adetola B. Adesida
Summary: This study investigated the time course of fibrocartilage formation by human meniscus fibrochondrocytes under various static conditions. Mechanical properties of the fibrocartilage were positively correlated with glycosaminoglycan content and accumulation of hyaline cartilage-like matrix components, and were lower in hypoxia but increased continually in normoxia. The most mechanically competent tissues reached a fraction of the native meniscus instantaneous compression modulus and exhibited a hypertrophic matrix-forming phenotype, which was consistently suppressed under hypoxia.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Correction
Multidisciplinary Sciences
Alexander R. A. Szojka, Colleen N. Moore, Yan Liang, Stephen H. J. Andrews, Melanie Kunze, Aillette Mulet-Sierra, Nadr M. Jomha, Adetola B. Adesida
Review
Cell Biology
Margaret J. Vyhlidal, Adetola B. Adesida
Summary: MFCs play a crucial role in regulating the biomechanical properties of the knee meniscus, and understanding their mechanotransductive mechanism is essential for advancing research in this field.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhiyao Ma, David Xinzheyang Li, Melanie Kunze, Aillette Mulet-Sierra, Lindsey Westover, Adetola B. Adesida
Summary: This study evaluated the effects of mechanical loading and unloading on the OA-related profile changes of engineered meniscus tissues and explored the biological sex-related differences. The results showed that mechanical stimulation had different effects on gene expression and tissue properties depending on the loading or unloading conditions and the sex of the donors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell Biology
Zhiyao Ma, Margaret J. Vyhlidal, David Xinzheyang Li, Adetola B. Adesida
Summary: This article reviews various mechanical stimuli applied in meniscus bioengineering over the past 10 years. By evaluating the effects of mechanical stimulation on bioengineered meniscus at both molecular and tissue levels, it provides an important foundation for meniscus tissue engineering and osteoarthritis modeling.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Engineering, Biomedical
Xiaoyi Lan, Adetola Adesida, Yaman Boluk
Summary: This article discusses the impact of rheological properties of collagen hydrogels on micro-extrusion and layer-stacking stages of bioprinting. It emphasizes the importance of steady shear flow measurements and large amplitude oscillatory shear tests in evaluating the performance of collagen networks during bioprinting. The article also introduces the significance of non-Newtonian material functions such as first normal stress difference and extensional viscosity. The discussions on collagen networks can also apply to the bioprinting of other hydrogels.
BIOMEDICAL MATERIALS
(2022)
Article
Multidisciplinary Sciences
Zhiyao Ma, David Xinzheyang Li, Ryan K. W. Chee, Melanie Kunze, Aillette Mulet-Sierra, Mark Sommerfeldt, Lindsey Westover, Daniel Graf, Adetola B. Adesida
Summary: Osteoarthritis primarily affects mechanical load-bearing joints, with the knee being the most common. Knee osteoarthritis (KOA) is more prevalent, burdensome, and severe in females, and hormonal differences do not fully explain this. Mechanical unloading in spaceflight microgravity has been implicated in the development of arthritis, but the mechanisms and sex differences are unclear. In this study, engineered meniscus constructs were generated and compared between male and female donors under normal gravity and simulated microgravity conditions to investigate the molecular basis and sex differences in the development of KOA.
Article
Multidisciplinary Sciences
A. K. Aissiou, S. Jha, K. Dhunnoo, Z. Ma, D. X. Li, R. Ravin, M. Kunze, K. Wong, A. B. Adesida
Summary: Spaceflight or simulated spaceflight microgravity can induce osteoarthritic-like alterations in the articular and meniscal cartilages of rodents, but its effect on tissue-engineered cartilage developed from human cells is not well understood. This study investigates the response of tissue-engineered cartilage to simulated microgravity facilitated by parabolic flights and reveals that the transcriptome profile and secretome of the engineered cartilage tissues showed sex-dependent differences in their modulation. These findings provide a foundation for further exploration of microgravity's underlying mechanisms.
Article
Engineering, Biomedical
Xiaoyi Lan, Zhiyao Ma, Melanie Kunze, Aillette Mulet-Sierra, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: Hydrogels with different crosslinking densities were used to investigate their effects on chondrocyte phenotype and cellular interaction. The results showed that the storage modulus of the hydrogel did not significantly alter the cartilaginous gene expression of chondrocytes. However, the gene expression of PPAR-gamma was positively correlated with crosslinking density. RNA-seq analysis revealed numerous genes that were significantly correlated to crosslinking density, which warrants further investigation.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Xiaoyi Lan, Yaman Boluk, Adetola B. Adesida
Summary: Due to the limited self-repair capacity of hyaline cartilage, tissue engineering strategy with 3D bioprinting technique has emerged as a new solution for hyaline cartilage repair and regeneration. This review summarizes the intrinsic property of hyaline cartilage and discusses the selection of bioprinters, bioink materials, and cell sources for successful bioprinting. The aim of this review is to identify current challenges and future directions for clinical development of bioprinted hyaline cartilage.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Cell & Tissue Engineering
David Xinzheyang Li, Zhiyao Ma, Alexander R. A. Szojka, Xiaoyi Lan, Melanie Kunze, Aillette Mulet-Sierra, Lindsey Westover, Adetola B. Adesida
Summary: This study introduced a novel protocol to differentiate hBM-MSC into stable, cartilage-forming cells. By combining mechano-hypoxia conditioning with TGF-beta 3 growth factor withdrawal, non-hypertrophic chondrogenesis of hBM-MSC can be promoted.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Cell & Tissue Engineering
Xiaoyi Lan, Yan Liang, Margaret Vyhlidal, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: This study investigated the impact of culture period on the formation and mechanical properties of 3D bioprinted constructs of engineered nasal cartilage using human nasal chondrocytes (hNC)-laden collagen hydrogel. The results demonstrated that these 3D bioprinted constructs were comparable or better than the clinically approved controls in terms of ECM formation and mechanical properties, making them feasible options for nasal cartilage reconstructive surgeries.
JOURNAL OF TISSUE ENGINEERING
(2022)
