Article
Biotechnology & Applied Microbiology
Liu Wu, Licheng Zhang, Xiaojie Li, Ruitong Lv, Wei Cao, Weixia Gao, Jiao Liu, Zhoujie Xie, Hao Liu
Summary: In this study, the heterologous production of kojic acid (KA) in Aspergillus niger was achieved through the expression of the kojA gene from Aspergillus oryzae. The study also identified four genes (nrkA, nrkB, nrkC, and nrkD) that negatively regulate KA production, and showed that knocking down nrkA or deleting any of the other three genes can significantly increase KA production. The highest KA titer of 25.71 g/L was achieved in a pH controlled batch bioreactor using the kojA overexpression strain with a deletion of nrkC, which showed a 26.7% improvement compared to shaking flask cultivation.
MICROBIAL CELL FACTORIES
(2023)
Article
Materials Science, Composites
Xicun Chai, Chunxia He, Yutao Liu, Evode Niyitanga, Yu Zhou, Luyang Wang, Weixing Zhang
Summary: This study investigated the degradation of wheat straw/PLA composites by Aspergillus niger with different contents of sodium alginate. The results showed that sodium alginate content within a certain range increased the mass loss rate of the composites, and the degraded composites had more hydroxyl and carbonyl groups. Moreover, the pyrolysis temperature and crystallinity of the composites significantly changed when the sodium alginate content was 9%.
POLYMER COMPOSITES
(2022)
Article
Food Science & Technology
Dong Zou, Jian Ji, Yongli Ye, Yang Yang, Jian Yu, Meng Wang, Yi Zheng, Xiulan Sun
Summary: In this study, a mutated strain of Aspergillus niger with a high degradation rate of ochratoxin A (OTA) was obtained and its degradation conditions were investigated. The degraded products and metabolic pathway of OTA were characterized using liquid chromatography-tandem mass spectrometry combined with mass spectrometry. The results showed that the degradation products had reduced toxicity on HepG2 cells. The application of this mutated strain in real samples demonstrated its effectiveness in removing OTA from wheat bran.
Article
Food Science & Technology
Yang Yang, Jian Ji, Shang Wu, Yongli Ye, Lina Sheng, Yinzhi Zhang, Xiulan Sun
Summary: In this study, the characteristics and mechanisms of patulin degradation by Aspergillus niger FS10 were evaluated using metabolomics technologies. The results showed that A. niger FS10 could effectively degrade 94.72% of patulin within 36 hours by producing intracellular enzymes that convert patulin into ascladiol. This degradation method reduces the damage caused by patulin to HepG2 cells and significantly affects the pentose phosphate pathway and the glutathione pathway. Furthermore, incubation of A. niger FS10 with patulin-contaminated apple pomace not only eliminates patulin but also increases the utilization of apple pomace.
Article
Biotechnology & Applied Microbiology
Zhi Li, Fangwang Shen, Lili Song, Shicui Zhang
Summary: NP20 is a novel antifungal peptide that has the ability to kill spores of Aspergillus niger and Aspergillus fumigatus and directly damage their surface, membrane, cytoplasm, organelle, and nucleus. It is active under temperature stress and a wide range of pH. The mechanisms underlying NP20's antifungal activity include binding with and passing through the fungal cell wall, interfering with the lipid membrane, inducing intracellular ROS production, DNA fragmentation, and protein synthesis inhibition of the fungal cells.
MARINE BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Yakov E. Dunaevsky, Valeriia F. Tereshchenkova, Mikhail A. Belozersky, Irina Y. Filippova, Brenda Oppert, Elena N. Elpidina
Summary: Celiac disease currently lacks an effective treatment, with strict adherence to a gluten-free diet sometimes not enough to alleviate symptoms. Enzyme therapy, as a pharmacological supplement, shows promise in breaking down gluten peptides but is not yet considered a standalone treatment for CD.
Article
Biotechnology & Applied Microbiology
Minglu Liu, Meng Hu, Hui Zhou, Zhiyang Dong, Xiuzhen Chen
Summary: In this study, recombinant expression of AN-PEP in Trichoderma reesei was achieved, resulting in the highest yield of rAN-PEP reported to date. It was also found that cultivating the recombinant strain on corn cob, a low-cost agricultural residue, significantly increased the secretion of rAN-PEP. Furthermore, treatment with rAN-PEP during beer brewing showed potential in reducing gluten content and improving beer stability.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Shu Zhang, Sha Zhao, Weihao Shang, Zijuan Yan, Xiuyun Wu, Yingjie Li, Guanjun Chen, Xinli Liu, Lushan Wang
Summary: Aspergillus niger An76 encodes a comprehensive set of xylan-degrading enzymes, including five endo-xylanases (one GH10 and four GH11). Quantitative transcriptional analysis showed that three xylanase genes (xynA, xynB, and xynC) were up-regulated by xylan substrates, with different secretion orders and amounts. Among the GH11 xylanases, XynB demonstrated the best performance in xylan degradation, and synergistic effects were observed between XynA and XynB.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Chemistry, Applied
Xiao Yu, Yanan Chen, Zhiguo Qi, Quan Chen, Yujin Cao, Qingshan Kong
Summary: The antioxidant activity of corn peptides is determined by their molecular weight and structure. Corn peptides with molecular weights less than 1 kDa (CPP1) show excellent antioxidant activity. A new peptide, Arg-Tyr-Leu-Leu (RYLL), has been identified from CPP1 and exhibits strong scavenging capacities for ABTS radicals and DPPH radicals. The study provides insights into the antioxidant mechanism of corn peptides and their potential as natural antioxidants in corn gluten meal (CGM) hydrolysates.
Article
Chemistry, Applied
Yan Ma, Tie-jun Ling, Xiao-qin Su, Bin Jiang, Bo Nian, Li-jiao Chen, Ming-li Liu, Zheng-yan Zhang, Dao-ping Wang, Yong-ying Mu, Wen-wen Jiao, Qian-ting Liu, Ying-hong Pan, Ming Zhao
Summary: The study revealed the important functions of Aspergillus in post-fermented Pu-erh tea, showing its ability to alter the concentrations and compositions of metabolites in tea leaves, mainly involving degradation and production of various compounds.
Article
Food Science & Technology
Sudarshanna Kar, P. P. Sutar
Summary: A two-stage sequential treatment method using ultrasound and microwave was developed to simultaneously inactivate POD, PPO, and Aspergillus niger in garlic bulbs. The optimized process achieved the desired levels of inactivation without compromising the quality of the garlic, and ultrasonication was found to enhance the efficacy of the microwave heating process.
FOOD AND BIOPROCESS TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wasana Wongngam, Ali Hamzeh, Fu Tian, Sittiruk Roytrakul, Jirawat Yongsawatdigul
Summary: Corn gluten meal was hydrolyzed to obtain a 3-10 kDa fraction containing ACE inhibitory peptides, with DLLGCS identified as the most active ACE inhibitor. Molecular docking analysis revealed that DLLGCS and CS peptides interacted with specific residues at the non-active site of ACE primarily through hydrogen bonding.
PROCESS BIOCHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Liyan Wang, Hao Guan, Jun Hu, Yi Feng, Xiang Li, Kianpoor Kalkhajeh Yusef, Hongjian Gao, Da Tian
Summary: This study investigated the mechanisms of wheat straw degradation and phosphorus release by the phosphate-solubilizing fungus Aspergillus niger. The results showed that A. niger significantly increased wheat straw degradation and released more phosphorus compared to the control group. The high enzyme activity and oxalic acid secretion by A. niger were identified as the main factors contributing to wheat straw degradation and phosphorus release.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Jie Qi, Xianzun Xiao, Liming Ouyang, Chenghan Yang, Yingping Zhuang, Lixin Zhang
Summary: This study suggests that glucoamylase production in A. niger can be significantly enhanced by overexpressing genes fadE and fadA involved in the fatty acid degradation pathway. Harnessing intracellular fatty acids may be a strategy to improve enzyme production in the cell factory of Aspergillus niger.
MICROBIAL CELL FACTORIES
(2022)
Article
Agricultural Engineering
Yangyang Li, Cen Li, Sahibzada Muhammad Aqeel, Yachan Wang, Quan Zhang, Jianing Ma, Jingwen Zhou, Jianghua Li, Guocheng Du, Song Liu
Summary: In this study, beta-glucan was successfully extracted from oat bran using a recombinant strain of Aspergillus niger AG11. The optimized expression cassette significantly improved the activity of the endogenous xylanase and amylolytic enzyme, resulting in a high purity of 85.1% beta-glucan. This research suggests that the Rbya strain has great potential for cost-effective extraction of beta-glucan.
BIORESOURCE TECHNOLOGY
(2023)
Article
Gastroenterology & Hepatology
J. Koenig, A. Siebenhaar, C. Hoegenauer, P. Arkkila, M. Nieuwdorp, T. Noren, C. Y. Ponsioen, U. Rosien, N. G. Rossen, R. Satokari, A. Stallmach, W. de Vos, J. Keller, R. J. Brummer
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2017)
Meeting Abstract
Gastroenterology & Hepatology
Savanne Holster, Robert J. Brummer, Dirk Repsilber, Julia Konig
Editorial Material
Gastroenterology & Hepatology
Julia Konig, Robert J. Brummer
EXPERT REVIEW OF GASTROENTEROLOGY & HEPATOLOGY
(2018)
Article
Gastroenterology & Hepatology
Savanne Holster, Carl Marten Lindqvist, Dirk Repsilber, Anne Salonen, Willem M. de Vos, Julia Konig, Robert J. Brummer
CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY
(2019)
Article
Biochemistry & Molecular Biology
Savanne Holster, Guido J. Hooiveld, Dirk Repsilber, Willem M. de Vos, Robert J. Brummer, Julia Konig
Editorial Material
Gastroenterology & Hepatology
Julia Konig, Robert Jan Brummer
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2020)
Article
Neurosciences
Julia Rode, Lin Yang, Julia Konig, Ashley Nicole Hutchinson, Rebecca Wall, Nikolaos Venizelos, Robert-Jan Brummer, Ignacio Rangel, Ravi Vumma
Summary: The study demonstrated that oxidative stress significantly reduced tryptophan uptake in fibroblast cells, which was countered by butyrate. Oxidative stress did not alter the gene expression profile of amino acid transporters, but treatment with different concentrations of butyrate differently regulated the gene expression of large amino acid transporters 1 and 2, the major transporters of tryptophan.
NEUROPSYCHOBIOLOGY
(2021)
Article
Microbiology
S. Holster, D. Repsilber, D. Geng, T. Hyotylainen, A. Salonen, C. M. Lindqvist, S. K. Rajan, W. M. de Vos, R. J. Brummer, J. Konig
Summary: The study found that allogenic FMT led to changes in microbial composition in IBS patients for up to six months, affecting functional profiles but not fecal metabolites. In contrast, autologous FMT had no effect on these factors. Correlations between microbial composition and metabolites also suggested disruptions in microbe-metabolite interactions after allogenic FMT compared to autologous FMT.
BENEFICIAL MICROBES
(2021)
Article
Multidisciplinary Sciences
Maria Fernanda Roca Rubio, Ulrika Eriksson, Robert J. Brummer, Julia Konig
Summary: The study revealed that acute intense psychological stress induced by skydiving does not affect intestinal permeability in healthy subjects. Future studies aiming to investigate the effect of stress on human intestinal barrier function should consider a more sustained exposure to the psychological stressor.
Article
Multidisciplinary Sciences
Maria Fernanda Roca Rubio, Ulrika Eriksson, Robert J. Brummer, Julia Konig
Summary: This study investigated the effect of sauna-induced dehydration on intestinal barrier function and found that sauna dehydration increased intestinal permeability without substantial damage to enterocytes. Additionally, it led to changes in inflammatory markers.
SCIENTIFIC REPORTS
(2021)
Article
Nutrition & Dietetics
Hanna M. T. Edebol Carlman, Julia Rode, Julia Konig, Dirk Repsilber, Ashley N. Hutchinson, Per Thunberg, Jonas Persson, Andrey Kiselev, Jens C. Pruessner, Robert J. Brummer
Summary: In this study, the impact of a probiotic product containing different strains of probiotics on stress processing was investigated. The results showed that probiotic intervention subtly altered brain activity and functional connectivity in specific brain regions and had a greater effect on the initial stress response. These findings support the potential of probiotics as a non-pharmaceutical treatment modality for stress-related disorders.
Article
Cell Biology
Julia Rode, Hanna M. T. Edebol Carlman, Julia Konig, Ashley N. Hutchinson, Per Thunberg, Jonas Persson, Robert J. Brummer
Summary: Probiotics can alter brain structure and function through the gut-brain axis, affecting psychological symptoms and sleep patterns. This study provides new insights into the ways in which gut microbiota can influence gut-brain communication and brain function.
Article
Gastroenterology & Hepatology
Savanne Holster, Julia Rode, Johan Bohr, Ashok Kumar Kumawat, Gabor Veress, Elisabeth Hultgren Hornquist, Robert Jan Brummer, Julia Konig
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2020)
Meeting Abstract
Gastroenterology & Hepatology
Julia Konig, Savanne Holster, Maaike J. Bruins, Robert J. Brummer