Article
Biochemistry & Molecular Biology
Jeng-Wei Lu, Liang-In Lin, Yuxi Sun, Dong Liu, Zhiyuan Gong
Summary: TWIST1 and KRAS play important roles in liver cancer metastasis. Using a transgenic zebrafish model, we found that co-expression of TWIST1a and KRAS, along with exposure to lipopolysaccharides, can enhance metastatic hepatocellular carcinoma.
Article
Biochemistry & Molecular Biology
Jeng-Wei Lu, Yuxi Sun, Pei-Shi Angelina Fong, Liang-In Lin, Dong Liu, Zhiyuan Gong
Summary: The study investigates the synergistic effects of kras(V12), lipopolysaccharides (LPS), and/or dextran sulfate sodium (DSS) on inflammation, tumor progression, and intestinal disorders using zebrafish. It found that the combination of KRAS mutations and LPS treatment can enhance intestinal tumor progression, providing insights into potential mechanisms for gastrointestinal cancers.
Article
Environmental Sciences
Yuchao Mao, Zijing Zong, Yao Dang, Liqin Yu, Chunsheng Liu, Jianghua Wang
Summary: The study found that MC-LR can exacerbate liver tumor progression and that environmental concentrations of MC-LR may promote hepatocellular carcinoma development, ultimately contributing to liver tumor progression.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Plant Sciences
Dan-Dan Wang, Pai Li, Qiu-Yi Chen, Xue-Ying Chen, Zi-Wei Yan, Mu-Yang Wang, Ying-Bo Mao
Summary: MYC-related genes play a critical role in Arabidopsis defense against cotton bollworm, with MYC3 having the greatest impact on the expression of genes involved in flavonoid biosynthesis. Flavonoids are suggested to alleviate the growth inhibition caused by wounding in Arabidopsis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Cell Biology
Mohammad Houshmand, Nicoletta Vitale, Francesca Orso, Alessandro Cignetti, Ivan Molineris, Valentina Gaidano, Stefano Sainas, Marta Giorgis, Donatella Boschi, Carmen Fava, Alice Passoni, Marta Gai, Massimo Geuna, Federica Sora, Alessandra Iurlo, Elisabetta Abruzzese, Massimo Breccia, Olga Mulas, Giovanni Caocci, Fausto Castagnetti, Daniela Taverna, Salvatore Oliviero, Fabrizio Pane, Marco Lucio Lolli, Paola Circosta, Giuseppe Saglio
Summary: The study shows that the newly developed DHODH inhibitor, Meds433, is highly effective in targeting CML cells by activating the apoptotic pathway and inducing metabolic stress. This finding suggests that DHODH inhibition is a promising approach for targeting CML stem/progenitor cells.
CELL DEATH & DISEASE
(2022)
Article
Biology
Bala Murali Krishna Vasamsetti, Kyongmi Chon, Ji-Yeong Choi, Juyeong Kim, Chang-Young Yoon
Summary: This study examined the developmental and transcriptomic effects of thiram exposure on zebrafish embryos. The thiram-exposed embryos exhibited developmental abnormalities and differential gene expression related to reproduction. These findings provide insights into the molecular mechanisms underlying thiram toxicity in zebrafish.
Article
Oncology
Denise Cecil, Kyong Hwa Park, Benjamin Curtis, Lauren Corulli, Mary (Nora) Disis
Summary: This study found that vaccine-induced type I T cells can inhibit tumor growth and stabilize disease, depending on IFN-γ-secreting CD4(+) T cells. Vaccination synergized with standard therapies, resulting in better treatment outcomes, and in some cases, complete resolution of the disease.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Review
Biochemistry & Molecular Biology
Shenglan Li, Can Wang, Jinyi Chen, Yanjie Lan, Weichunbai Zhang, Zhuang Kang, Yi Zheng, Rong Zhang, Jianyu Yu, Wenbin Li
Summary: Brain tumors, though rare, have a significant impact on mortality and morbidity across all age groups. Advances in targeted therapies and immunotherapies have improved prognoses, but response rates and drug resistance remain obstacles to further progress. Dysregulation of signaling pathways in brain tumors presents an opportunity for novel targeted therapies. A comprehensive understanding of pathogenesis is urgently needed to develop effective therapeutic approaches. This paper provides an overview of epidemiology, signaling pathways, current therapies, and future challenges in brain tumor treatment.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Peter Macsek, Jan Skoda, Maria Krchniakova, Jakub Neradil, Renata Veselska
Summary: Novel thiosemicarbazones show promise in targeting MYCN-amplified and non-amplified neuroblastoma by reducing N-MYC and c-MYC expression, and decreasing total EGFR levels and phosphorylation through the involvement of NDRG1, a positive prognostic marker in neuroblastoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Haisong Bu, Yonghe Ding, Jiarong Li, Ping Zhu, Yu-Huan Shih, Mingmin Wang, Yuji Zhang, Xueying Lin, Xiaolei Xu
Summary: Research identified vmhcl as a zebrafish homolog of human MYH7, with therapeutic benefits of mTOR or MAPK inhibition on VEC model. These findings were later validated in VAC model, offering a rapid in vivo platform for discovering candidate signaling pathways of MYH7 cardiomyopathy.
Article
Biochemistry & Molecular Biology
Qizhi Liu, Stacey Chung, Michael M. Murata, Bingchen Han, Bowen Gao, Maoqi Zhang, Tian-Yu Lee, Evgeny Chirshev, Juli Unternaehrer, Hisashi Tanaka, Armando E. Giuliano, Yukun Cui, Xiaojiang Cui
Summary: Two distinct and diverging cancer cell responses to TOP1 inhibitors were observed in TNBC, with one group being sensitive and the other resistant. The activation patterns of ATR/Chk1 and JNK, as well as the expression of MYC, may serve as predictive markers for cancer cell response to TOP1 inhibitors. The constitutive elevation of MYC expression mediated by JNK activation may represent a novel mechanism governing cancer cell sensitivity to TOP1-targeting therapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yiqun Zhang, Fengju Chen, Darshan S. Chandrashekar, Sooryanarayana Varambally, Chad J. Creighton
Summary: Pan-cancer proteomics analysis reveals 11 distinct subtypes across multiple cancer types, linking pathway activity levels with somatic alteration of specific genes. The interplay between the genome and proteome is observed, and MYC pathway activity is found in cancers without MYC copy gain but with mutations in genes with noncanonical roles in MYC.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Giulia Franciosa, Jos G. A. Smits, Sonia Minuzzo, Ana Martinez-Val, Stefano Indraccolo, Jesper V. Olsen
Summary: NOTCH1 is a crucial oncogenic driver in T-cell acute lymphoblastic leukemia and can be inhibited by GSIs, but their clinical efficacy is limited. Through quantitative mass spectrometry-based phosphoproteomics, common mechanisms of GSI resistance were identified, and a PKC inhibitor sotrastaurin was shown to enhance the anti-leukemic activity of GSI and prevent the development of acquired resistance. Proteomics has the potential to dissect alterations in cellular signaling and identify druggable pathways in cancer.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Julie K. Monda, Xuezhen Ge, Moritz Hunkeler, Katherine A. Donovan, Michelle W. Ma, Cyrus Y. Jin, Marilyn Leonard, Eric S. Fischer, Eric J. Bennett
Summary: HUWE1 is a large, enigmatic HECT-domain ubiquitin ligase involved in the regulation of various pathways. The specific interaction between HUWE1 and its substrates and the regulation of HUWE1 activity are still unknown. Through quantitative proteomics, HUWE1 is found to mainly target substrates in a cell-type-specific manner, but C16orf72/HAPSTR1 is identified as a consistent substrate in multiple cell lines. The physical and genetic interactions between HUWE1 and HAPSTR1 suggest that HAPSTR1 positively regulates HUWE1 function, particularly in nuclear localization and substrate targeting.
Article
Cell Biology
Alessio Paolini, Federica Fontana, Van-Cuong Pham, Claudia Jasmin Roedel, Salim Abdelilah-Seyfried
Summary: In the zebrafish embryo, blood flow generates fluid shear stress on endocardial cells, activating Notch and Klf2 signaling pathways crucial for atrioventricular valvulogenesis. Lateral inhibition mediated by Notch selects Delta-like-4-positive endocardial cells to ingress into the cardiac jelly and form an abluminal cell population. Wnt9a signaling cascade, activated by blood flow through Erk5Klf2-Wnt9a pathway, drives Delta-like-4-positive cells towards either luminal or abluminal fates, ultimately sculpting cardiac valve leaflets.
Article
Cell Biology
Qiqi Yang, Chuan Yan, Xu Wang, Zhiyuan Gong
DISEASE MODELS & MECHANISMS
(2019)
Article
Oncology
E. G. Garcia, A. Veloso, M. L. Oliveira, J. R. Allen, S. Loontiens, D. Brunson, D. Do, C. Yan, R. Morris, S. Iyer, S. P. Garcia, N. Iftimia, W. Van Loocke, F. Matthijssens, K. McCarthy, J. T. Barata, F. Speleman, T. Taghon, A. Gutierrez, P. Van Vlierberghe, W. Haas, J. S. Blackburn, D. M. Langenau
Summary: The study identifies PRL3 as a collaborating oncogenic driver in T-ALL, which synergizes with MYC to promote leukemia development and suppress apoptosis in cells. By suppressing downstream phosphorylation signaling pathways, PRL3's role in T-ALL is elucidated.
Article
Biochemical Research Methods
Chuan Yan, Daniel Do, Qiqi Yang, Dalton C. Brunson, John F. Rawls, David M. Langenau
Article
Multidisciplinary Sciences
Mohamed Helal, Chuan Yan, Zhiuyan Gong
Summary: The study reveals that tumor progression is influenced by factors secreted by both tumor cells and surrounding non-tumor cells. Through an inducible transgenic zebrafish model, it was found that cholangiocytes play a promoting role in hepatocarcinogenesis by enhancing cell proliferation via an inflammatory loop involving S1pr2 and Il17a/f1.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Chuan Yan, Qiqi Yang, Songfa Zhang, David G. Millar, Eric J. Alpert, Daniel Do, Alexandra Veloso, Dalton C. Brunson, Benjamin J. Drapkin, Marcello Stanzione, Irene Scarfo, John C. Moore, Sowmya Iyer, Qian Qin, Yun Wei, Karin M. McCarthy, John F. Rawls, Nick J. Dyson, Mark Cobbold, Marcela Maus, David M. Langenau
Summary: This study utilized optically clear immunocompromised zebrafish models engrafted with fluorescent-labeled human cancers and various T cell immunotherapies to visualize T cell immune responses at single-cell resolution in real time. Differences in kinetics of T cell infiltration, tumor cell engagement, and killing were identified among the immunotherapies, leading to early endpoint analysis for predicting therapy responses. EGFR-targeted immunotherapies were established as a powerful approach to eradicate rhabdomyosarcoma muscle cancers, suggesting the potential of assessing a broader range of T cell immunotherapies in this disease.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Oncology
Songfa Zhang, Chuan Yan, David G. Millar, Qiqi Yang, James M. Heather, Adam Langenbucher, Laura T. Morton, Sean Sepulveda, Eric Alpert, Lauren R. Whelton, Dominique T. Zarrella, Mei Guo, Eleanor Minogue, Michael S. Lawrence, Bo R. Rueda, David R. Spriggs, Weiguo Lu, David M. Langenau, Mark Cobbold
Summary: This study develops a high-throughput preclinical platform to identify patient-specific antibody-peptide epitope conjugates that target cancer cells and demonstrates the potential of this immunotherapy approach for treating ovarian carcinoma.
Article
Biology
Maja Solman, Sasja Blokzijl-Franke, Florian Piques, Chuan Yan, Qiqi Yang, Marion Strullu, Sarah M. Kamel, Pakize Ak, Jeroen Bakkers, David M. Langenau, Helene Cave, Jeroen den Hertog
Summary: This study discovers a common inflammatory response in sporadic JMML patients and syndromic NS zebrafish, which may potentiate the development of MPN and serve as a potential target for JMML therapies.
Correction
Biotechnology & Applied Microbiology
Qiqi Yang, Lyana Salim, Chuan Yan, Zhiyuan Gong
MARINE BIOTECHNOLOGY
(2023)
Article
Hematology
Saara Laukkanen, Alexandra Veloso, Chuan Yan, Laura Oksa, Eric J. Alpert, Daniel Do, Noora Hyvarinen, Karin McCarthy, Abhinav Adhikari, Qiqi Yang, Sowmya Iyer, Sara P. Garcia, Annukka Pello, Tanja Ruokoranta, Sanni Moisio, Sadiksha Adhikari, Jeffrey A. Yoder, Kayleigh Gallagher, Lauren Whelton, James R. Allen, Alex H. Jin, Siebe Loontiens, Merja Heinaniemi, Michelle Kelliher, Caroline A. Heckman, Olli Lohi, David M. Langenau
Summary: This study identified a drug combination of AKT/mTORC1 inhibitors and dasatinib that effectively kills T-cell acute lymphoblastic leukemia (T-ALL) in zebrafish, including relapse and dexamethasone-resistant cases. Clinical trials have shown promising results, indicating that this combination could be an effective therapy for T-ALL in humans.
Article
Oncology
Yun Wei, Qian Qin, Chuan Yan, Madeline N. Hayes, Sara P. Garcia, Haibin Xi, Daniel Do, Alexander H. Jin, Tiffany C. Eng, Karin M. McCarthy, Abhinav Adhikari, Maristela L. Onozato, Dimitrios Spentzos, Gunnlaugur P. Neilsen, A. John Iafrate, Leonard H. Wexler, April D. Pyle, Mario L. Suva, Filemon Dela Cruz, Luca Pinello, David M. Langenau
Summary: In this study, researchers used single-cell RNA sequencing and functional characterization to uncover the similarities between tumor cell hierarchies in rhabdomyosarcoma (RMS) and human muscle development. They also identified molecularly defined tumor-propagating subpopulations in RMS. These findings have important implications for understanding and treating RMS.
Review
Oncology
Maurizio Fazio, Julien Ablain, Yan Chuan, David M. Langenau, Leonard I. Zon
NATURE REVIEWS CANCER
(2020)
