Review
Biochemistry & Molecular Biology
Awa Diop, Daniele Santorelli, Francesca Malagrino, Caterina Nardella, Valeria Pennacchietti, Livia Pagano, Lucia Marcocci, Paola Pietrangeli, Stefano Gianni, Angelo Toto
Summary: This review summarizes the latest research on the structure, function, pathogenic role, and regulation of SH2 domains. The findings suggest that pharmacological interest in SH2 domains and a deeper understanding of their binding properties are crucial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Qilin Yu, William R. Holmes, Jean P. Thiery, Rodney B. Luwor, Vijay Rajagopal
Summary: Adherens junctions physically connect two cells through the binding of cadherin molecules and actin cytoskeleton. Mechanical and chemical signals regulate the dynamics of cadherin and actomyosin cytoskeleton, determining the strength of cell-cell adhesion and tissue organization. This study presents a computational model investigating the mechanochemical cross talk that regulates adherens junction formation and homeostasis, and proposes a positive feedback loop between cadherin and actin as a mechanism for the formation of the ring structure.
BIOPHYSICAL JOURNAL
(2022)
Review
Cell Biology
Mehrnaz Mehrabipour, Neda S. Kazemein Jasemi, Radovan Dvorsky, Mohammad R. Ahmadian
Summary: SRC homology 3 (SH3) domains are modular building blocks that facilitate the assembly of protein complexes through interactions with proline-rich/noncanonical motifs. They have been extensively studied across different life kingdoms and viruses, playing roles in various biological processes. Additionally, SH3 domains are implicated in the development of human diseases and are potential drug targets. This review summarizes the classification of SH3 domain functions, their binding specificity, and their significance in disease research.
Review
Neurosciences
Davide Comoletti, Laura Trobiani, Arnaud Chatonnet, Yves Bourne, Pascale Marchot
Summary: Cell adhesion involves the formation of protein complexes between two cells, with neural cell-adhesion proteins using cholinesterase-like domains to bind cognate partners. These proteins, including neuroligins, have unique structural features but share the ability to recognize and bind protein partners.
Article
Medicine, Research & Experimental
Xiaoyang Qi, Ziying Sun, Xugang Li, Yuwen Jiao, Shuai Chen, Peng Song, Zhifen Qian, Jun Qian, Xusheng Qiu, Liming Tang
Summary: The study found that after sleeve gastrectomy, triglyceride, leptin, PPAR gamma, and Cebp alpha levels were significantly reduced in the adipose tissue of rats. Shp2 expression levels decreased following leptin treatment, and the silencing or inhibition of Shp2 led to depot-specific adipogenesis in ingWAT pre-adipocytes. Conversely, Shp2 overexpression decreased the expression of adipogenic markers by enhancing beta-catenin expression.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Oncology
Aibin Liu, Huayan Xie, Ronggang Li, Liangliang Ren, Baishuang Yang, Longxia Dai, Wenjie Lu, Baoyi Liu, Dong Ren, Xin Zhang, Qiong Chen, Yanming Huang, Ke Shi
Summary: ZIC2 was found to be upregulated in NSCLC tissues and high expression of ZIC2 correlated with worse overall and progression-free survival in NSCLC patients. Silencing ZIC2 inhibited tumorigenesis and reduced anoikis resistance in NSCLC cells. Further investigation revealed that silencing ZIC2 transcriptionally inhibited Src expression and inactivated steroid receptor coactivator/focal adhesion kinase signaling, thereby attenuating anoikis resistance in NSCLC cells.
MOLECULAR THERAPY-ONCOLYTICS
(2021)
Article
Oncology
Manali Tilak, Begum Alural, Sarah E. Wismer, Megan I. Brasher, Laura A. New, Steven D. Sheridan, Roy H. Perlis, Marc G. Coppolino, Jasmin Lalonde, Nina Jones
Summary: This study identified the upregulation of ShcD in malignant gliomas and its association with Tie2 receptor tyrosine kinase to promote invasion. The expression of ShcD and Tie2 in glioma cells enhances invasion, with ShcD mutant disrupting the synergistic effect. The elevated expression of ShcD correlates with poor patient outcomes in advanced gliomas.
MOLECULAR CANCER RESEARCH
(2021)
Article
Biochemical Research Methods
Allison Maker, Barry M. Gumbiner
Summary: This study reconstitutes the complete and full-length cadherin-catenin complex and observes it using nanodiscs and cryo-EM. The affinities of the binding interactions between alpha-catenin, beta-catenin, p120-catenin, and the E-cadherin cytoplasmic tail are analyzed. The results reveal the role of p120-catenin in adhesion activation.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Biochemistry & Molecular Biology
Zhonglei Bao, Jianghua Liu, Jin Fu
Summary: This study systematically created a comprehensive binary interaction map of all tau phosphotyrosine sites with SH2 domains in the human genome and identified strong and moderate binding pairs. The research found that the SH2-recognition specificity of different tau phosphotyrosine sites has been optimized evolutionarily and may regulate different but partially overlapped biological functions in tau signaling. Some SH2 groups, such as SRC, RIN, PLCG, SOCS, and SH2D, showed effective binding potency compared to others and could be potential tau-associated proteins in downstream signaling.
Article
Cell Biology
Daniel Kummer, Tim Steinbacher, Sonja Tholmann, Mariel Flavia Schwietzer, Christian Hartmann, Simone Horenkamp, Sabrina Demuth, Swetha S. D. Peddibhotla, Frauke Brinkmann, Bjorn Kemper, Jurgen Schnekenburger, Matthias Brandt, Timo Betz, Ivan Liashkovich, Ivan U. Kouzel, Victor Shahin, Nathalie Corvaia, Klemens Rottner, Katsiaryna Tarbashevich, Erez Raz, Lilo Greune, M. Alexander Schmidt, Volker Gerke, Klaus Ebnet
Summary: This study identifies the cell adhesion molecule JAM-A as a central regulator of contact inhibition of locomotion (CIL) in tumor cells. JAM-A links to αvβ5 integrin through tetraspanins CD9 and CD81, and inhibits the activity of Src. Loss of JAM-A leads to increased motility, enhanced cell-matrix turnover, and failure to halt migration upon collision with other cells. Proper regulation of CIL also depends on αvβ5 integrin engagement.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biophysics
Bright Shi, Tsutomu Matsui, Shuo Qian, Thomas M. Weiss, Iain D. Nicholl, David J. E. Callaway, Zimei Bu
Summary: The cell-cell adhesion cadherin-catenin complexes recruit vinculin to the adherens junction (AJ), but the specific influence of vinculin on AJ structure and function remains unclear. This study identifies two patches of salt bridges that lock vinculin in an autoinhibited conformation and reconstitutes the vinculin activation mimetics bound to the cadherin-catenin complex. The dynamic cadherin-catenin-vinculin complex employs vinculin as the primary F-actin binding mode to strengthen AJ-cytoskeleton interactions.
BIOPHYSICAL JOURNAL
(2023)
Article
Cell Biology
Indrajyoti Indra, Regina B. Troyanovsky, Kathleen J. Green, Sergey M. Troyanovsky
Summary: In this study, the role of the desmosomal ARM protein Pkp3 in integrating desmosomes into the apical cell junctional complex (AJC) was investigated using polarized DLD1 cells. The data suggest that Par3 facilitates DSM assembly within the AJC and controls the availability of Pkp3 in tricellular contacts, highlighting the importance of apical polarity proteins in desmosome localization.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Microbiology
Ricardo Estevens, Dalila Mil-Homens, Arsenio M. Fialho
Summary: This study discusses the adhesion molecules in Burkholderia cenocepacia and identifies a new type of adhesion factors called bacterial collagen-like proteins. These proteins are formed by compositionally biased residues within intrinsically disordered regions, which may enhance their efficiency as adhesion factors.
Article
Biochemistry & Molecular Biology
Shotaro Sakakibara, Ayuko Sakane, Takuya Sasaki, Masakazu Shinohara, Tomohiko Maruo, Muneaki Miyata, Kiyohito Mizutani, Yoshimi Takai
Summary: This study reveals the role of lysophosphatidic acid (LPA) in promoting the organization of the apical junctional complex (AJC). LPA acts through the LPA receptor 1/5 and activates the diacylglycerol-novel PKC and Rho-ROCK pathways to mediate AJC organization, independently of myosin II-induced actomyosin contraction.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Richard N. Day, Kathleen H. Day, Fredrick M. Pavalko
Summary: The study identified a putative mechanosome involving Src, Pyk2, and MBD2 in MLO-Y4 osteocytes using FRET-FLIM technology. Src-Pyk2 complexes were concentrated at peripheral focal adhesions and the peri-nuclear region, while Pyk2-MBD2 complexes were mainly located in the nucleus and peri-nuclear region. Mechanical stimulation by fluid flow induced accumulation of Src-Pyk2 protein complexes in the peri-nuclear/nuclear region, supporting the proposed behavior of a mechanosome in response to mechanical stimuli.
Letter
Oncology
Cyril Garcia, Francoise Cormier, Lionel Groussin
CLINICAL CANCER RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Gregory Lazarian, Chloe Friedrich, Anne Quinquenel, Julie Tran, Souhail Ouriemmi, Elisabetta Dondi, Antoine Martin, Imane Mihoub, David Chiron, Celine Bellanger, Carole Fleury, Pascal Gelebart, Emmet McCormack, Dominique Ledoux, Catherine Thieblemont, Jacek Marzec, John G. Gribben, Florence Cymbalista, Nadine Varin-Blank, Laura Gardano, Fanny Baran-Marszak
Article
Cell Biology
Elisabetta Dondi, Jean-Baptiste Sibarita, Nadine Varin-Blank, Laura Velazquez
CELLULAR SIGNALLING
(2020)
Article
Rheumatology
Jonathan London, Nicolas Dumoitier, Sebastien Lofek, Jeremie Dion, Benjamin Chaigne, Julie Mocek, Nathalie Thieblemont, Pascal Cohen, Claire Le Jeunne, Loic Guillevin, Veronique Witko-Sarsat, Nadine Varin-Blank, Benjamin Terrier, Luc Mouthon
Summary: The study revealed that patients with GPA have skewed T-cell polarization towards Th2, Th9, and Th17 responses, while B-cell populations in both GPA and MPA exhibit an activated phenotype and decreased B-cell activating factor-receptor expression.
Article
Cell Biology
Rofia Boudria, Vanessa Lauriente, Antonin Oudar, Souleymane Harouna-Rachidi, Elisabetta Dondi, Christine Le Roy, Laura Gardano, Nadine Varin-Blank, Lionel Guittat
Summary: Vav1 interacts with Syk in lung cancer cells, collectively regulating the phosphorylation status of beta-catenin and impacting cell migration ability.
CELLULAR SIGNALLING
(2021)
Article
Multidisciplinary Sciences
Miriam Lisci, Philippa R. Barton, Lyra O. Randzavola, Claire Y. Ma, Julia M. Marchingo, Doreen A. Cantrell, Vincent Paupe, Julien Prudent, Jane C. Stinchcombe, Gillian M. Griffiths
Summary: This study revealed that CTLs require mitochondria for target cell-killing, and this requirement is linked to mitochondrial translation. Inhibition of mitochondrial translation impairs CTL killing by triggering attenuated cytosolic translation, leading to a reduced capacity for sustained killing. Mitochondria emerge as a previously unappreciated homeostatic regulator of protein translation required for serial CTL killing.
Article
Oncology
Chahrazade Kantari-Mimoun, Sarah Barrin, Lene Vimeux, Sandrine Haghiri, Claire Gervais, Sandy Joaquina, Joerg Mittelstaet, Nadine Mockel-Tenbrinck, Ali Kinkhabwala, Diane Damotte, Audrey Lupo, Mathilde Sibony, Marco Alifano, Elisabetta Dondi, Nadege Bercovici, Alain Trautmann, Andrew D. Kaiser, Emmanuel Donnadieu
Summary: The study found that CD20 CAR-T cells rapidly formed effective conjugates with target cells, while EGFR CAR-T cells need to migrate to the center of tumor cell regions after initial activation at the periphery of tumor islets. Activated CAR-T cells induce upregulation of ICAM-1 on malignant tumor cells via an IFN gamma-dependent pathway.
CANCER IMMUNOLOGY RESEARCH
(2021)
Article
Biology
Lyra O. Randzavola, Paige M. Mortimer, Emma Garside, Elizabeth R. Dufficy, Andrea Schejtman, Georgia Roumelioti, Lu Yu, Mercedes Pardo, Kerstin Spirohn, Charlotte Tolley, Cordelia Brandt, Katherine Harcourt, Esme Nichols, Mike Nahorski, Geoff Woods, James C. Williamson, Shreehari Suresh, John M. Sowerby, Misaki Matsumoto, Celio X. C. Santos, Cher Shen Kiar, Subhankar Mukhopadhyay, William M. Rae, Gordon J. Dougan, John Grainger, Paul J. Lehner, Michael A. Calderwood, Jyoti Choudhary, Simon Clare, Anneliese Speak, Giorgia Santilli, Alex Bateman, Kenneth G. C. Smith, Francesca Magnani, David C. Thomas
Summary: EROS protein plays a crucial role in immune response by regulating the maturation of gp91phox and the function of purine receptors P2X7 and P2X1. Its deficiency results in severe immunodeficiency and chronic granulomatous disease, while also enhancing resistance to influenza infection.
Article
Hematology
Rim Zaaboub, Lene Vimeux, Vincent Contremoulins, Florence Cymbalista, Vincent Levy, Emmanuel Donnadieu, Nadine Varin-Blank, Antoine Martin, Elisabetta Dondi
Summary: The tumor microenvironment plays a critical role in the survival and treatment resistance of CLL cells. Through the interaction of chemokines, chemokine receptors, and specific cells, the tumor microenvironment provides a protective niche for tumor cells, allowing them to evade treatment.