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Biochemistry & Molecular Biology
Yaiza R. Varela, Emilio J. Gonzalez-Ramirez, Marina N. Iriondo, Uxue Ballesteros, Asier Etxaniz, Lidia Ruth Montes, Felix M. Goni, Alicia Alonso
Summary: Cardiolipin (CL) is an important lipid for damaged mitochondrial recognition by the LC3/GABARAP autophagy proteins. The role of ceramide (Cer) is uncertain, but it has been proposed that CL and Cer may coexist in mitochondria. This study examined the interaction of LC3/GABARAP proteins with model membranes containing eSM, DOPE, CL, and/or Cer. The results suggest that Cer-enriched rigid nanodomains within the fluid phase may facilitate LC3/GABARAP protein interaction by creating structural defects at the nanointerfaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Cell Biology
Yaiza R. Varela, Alicia Alonso
Summary: The interaction between LC3/GABARAP proteins and specific lipids plays a crucial role in their function. Cardiolipin and ceramide have been found to be essential for the protein-bilayer interaction.
Review
Cell Biology
Marine Jacquet, Michael Guittaut, Annick Fraichard, Gilles Despouy
Summary: Atg8-family proteins, including GABARAP and LC3, play crucial roles in autophagy and cancer. While downregulation of GABARAPs is associated with cancer progression, the expression of LC3s does not correlate with specific tumor types or stages. The exact role of Atg8-family proteins in cancer remains unclear, but it seems to be related to their involvement in selective protein degradation through autophagy.
Article
Cell Biology
Isaac Tamargo-Gomez, Gemma G. Martinez-Garcia, Maria F. Suarez, Pablo Mayoral, Gabriel Bretones, Aurora Astudillo, Jesus Prieto-Lloret, Christina Sveen, Antonio Fueyo, Nikolai Engedal, Carlos Lopez-Otin, Guillermo Marino
Summary: The study of mutant mice deficient in Atg4c reveals the important role of Atg4c in tissue-specific autophagy and cellular immunity.
Editorial Material
Cell Biology
Shuhei Nakamura, Shiori Akayama, Tamotsu Yoshimori
Summary: Lysosomes play a crucial role in cellular digestion and maintaining cellular homeostasis. Cells have developed defense mechanisms, including the regulation of transcription factors like TFEB and LC3, to cope with damaged lysosomes.
Article
Biochemistry & Molecular Biology
Yaiza R. Varela, Marina N. Iriondo, Asier Etxaniz, Uxue Ballesteros, L. Ruth Montes, Felix M. Goni, Alicia Alonso
Summary: This study explores the interaction of LC3/GABARAP proteins with phospholipid monolayers and bilayers. It finds that cardiolipin is essential for the protein interaction with bilayers, while ceramide markedly increases binding. Confocal fluorescence microscopy experiments reveal the arrangement of ceramide in the phospholipid bilayers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Thanh Ngoc Nguyen, Michael Lazarou
Summary: This study reconciles prior observations of the core ATG8 system into a unifying model, and discusses bypass pathways of autophagy that function independently of the core ATG8 system.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Timothee Baudu, Chloe Parratte, Valerie Perez, Marie Ancion, Stefania Millevoi, Eric Hervouet, Anne Peigney, Paul Peixoto, Alexis Overs, Michael Herfs, Annick Fraichard, Michael Guittaut, Aurelie Baguet
Summary: This study identified a potential link between ATG8 family members regulation by NMD and their involvement in tumor development and metastasis formation. The findings suggest that 3' UTR-dependent NMD may play a role in autophagy induction and could be a promising target for new anti-cancer therapies.
Article
Biochemistry & Molecular Biology
Celia Fourrier, Valerie Bryksin, Kathryn Hattersley, Leanne K. Hein, Julien Bensalem, Timothy J. Sargeant
Summary: Measurement of autophagic flux in the brain remains a challenging research topic, with current methods not fully effective in capturing autophagic activity in this specific tissue. More research is needed to find a suitable drug for this purpose.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Gemma G. Martinez-Garcia, Raul F. Perez, Alvaro F. Fernandez, Sylvere Durand, Guido Kroemer, Guillermo Marino
Summary: Autophagy is crucial for maintaining cellular and tissue homeostasis, and dysfunction can lead to various pathologies. The metabolic effects of systemic reduction of autophagy are tissue-dependent, with skeletal muscle and plasma showing the most pronounced alterations. This study sheds light on how impaired autophagy may impact the metabolism of different tissues in mammals.
Article
Cell Biology
Kaiyuan Wu, Allen Seylani, Jing Wu, Xufeng Wu, Christopher K. E. Bleck, Michael N. Sack
Summary: The functional interaction of BLOC1S1 with the kinesin binding complex and the actin cytoskeleton are essential for lysosomal tubulation, which plays a key role in lysosome recycling through autophagic lysosome reformation (ALR).
Review
Cell Biology
Jose L. Nieto-Torres, Sviatlana Zaretski, Tianhui Liu, Peter D. Adams, Malene Hansen
Summary: This review summarizes the recent discoveries of post-translational regulatory events that influence the autophagy process, and discusses their roles in physiology and disease.
JOURNAL OF CELL SCIENCE
(2023)
Review
Cell Biology
Trond Lamark, Terje Johansen
Summary: Selective autophagy is the degradation of specific intracellular components via selective autophagy receptors, which interact with autophagy-related proteins and may or may not depend on ubiquitin labeling of the cargo.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 37
(2021)
Editorial Material
Cell Biology
Marina N. Iriondo, Alicia Alonso
Summary: Recently, we investigated the membrane anchoring and lipidation process of six members of the LC3/GABARAP protein family and their ability to promote membrane tethering and fusion. GABARAP and GABARAPL1 exhibited the highest activities. Differences within the LC3/GABARAP proteins indicated the requirement of a lipidation threshold for tethering and inter-vesicular lipid mixing. The presence of ATG12-ATG5-ATG16L1 (E3) increased and accelerated LC3/GABARAP lipidation and vesicle tethering, but hindered their capacity for inter-vesicular lipid mixing and fusion. Our findings suggest that LC3/GABARAP proteins contribute to phagophore expansion through vesicle tethering and fusion, in addition to the recently described inter-membrane lipid transfer mechanism.
Article
Pharmacology & Pharmacy
Tao Wang, Li Wang, Yi Zhang, Jian Sun, Yilin Xie, Yan Yuan, Jianhong Gu, Jianchun Bian, Zongping Liu, Hui Zou
Summary: This study demonstrates that puerarin alleviates cadmium-induced hepatocyte injury by activating autophagy and mitigating autophagy blockade. Through investigating a cell model exposed to cadmium, it was found that puerarin enhanced autophagic activity, strengthened lysosomal degradation capacity, and restored the fusion of autophagosomes and lysosomes by upregulating Rab7 expression.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Mario Mauthe, Idil Orhon, Cecilia Rocchi, Xingdong Zhou, Morten Luhr, Kerst-Jan Hijlkema, Robert P. Coppes, Nikolai Engedal, Muriel Mari, Fulvio Reggiori
Article
Cell Biology
Nikolai Engedal, Eva Zerovnik, Alexander Rudov, Francesco Galli, Fabiola Olivieri, Antonio Domenico Procopio, Maria Rita Rippo, Vladia Monsurro, Michele Betti, Maria Cristina Albertini
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2018)
Review
Cell Biology
M. Pajares, A. Cuadrado, N. Engedal, Z. Jirsova, M. Cahova
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2018)
Editorial Material
Cell Biology
Maria C. Albertini, Tassula Proikas-Cezanne, Nikolai Engedal, Eva Zerovnik, Jon D. Lane
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2018)
Article
Cell Biology
Paula Szalai, Jan B. Parys, Geert Bultynck, Soren Brogger Christensen, Poul Nissen, Jesper Moller, Nikolai Engedal
Article
Nanoscience & Nanotechnology
Marzena Szwed, Tonje Sonstevold, Anders Overbye, Nikolai Engedal, Beata Grallert, Yrr Morch, Einar Sulheim, Tore-Geir Iversen, Tore Skotland, Kirsten Sandvig, Maria L. Torgersen
Article
Biochemistry & Molecular Biology
Morten Luhr, Maria Lyngaas Torgersen, Paula Szalai, Adnan Hashim, Andreas Brech, Judith Staerk, Nikolai Engedal
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell Biology
Yan Zhen, Helene Spangenberg, Michael J. Munson, Andreas Brech, Kay O. Schink, Kia-Wee Tan, Vigdis Sorensen, Eva Maria Wenzel, Maja Radulovic, Nikolai Engedal, Anne Simonsen, Camilla Raiborg, Harald Stenmark
Article
Cell Biology
Paula Lindner, Soren Brogger Christensen, Poul Nissen, Jesper Vuust Moller, Nikolai Engedal
CELL COMMUNICATION AND SIGNALING
(2020)
Article
Multidisciplinary Sciences
S. Taavitsainen, N. Engedal, S. Cao, F. Handle, A. Erickson, S. Prekovic, D. Wetterskog, T. Tolonen, E. M. Vuorinen, A. Kiviaho, R. Natkin, T. Hakkinen, W. Devlies, S. Henttinen, R. Kaarijarvi, M. Lahnalampi, H. Kaljunen, K. Nowakowska, H. Syvala, M. Blauer, P. Cremaschi, F. Claessens, T. Visakorpi, T. L. J. Tammela, T. Murtola, K. J. Granberg, A. D. Lamb, K. Ketola, I. G. Mills, G. Attard, W. Wang, M. Nykter, A. Urbanucci
Summary: The authors identified chromatin and gene expression patterns in response to enzalutamide treatment in prostate cancer using single cell-ATAC and RNAseq techniques. They found distinct chromatin landscapes associated with treatment and resistance, which could predict treatment response. Ultimately, these findings may inform clinical decision-making by revealing molecular predictors of treatment response in pre-clinical models.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Cell Biology
Nikolai Engedal, Tassula Proikas-Cezanne, Maria C. Albertini, Eva Zerovnik, Jon D. Lane
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Cell Biology
Tonje Sonstevold, Nikolai Engedal, Maria Lyngaas Torgersen
Summary: The nanoparticle drug delivery vehicle poly(butyl cyanoacrylate) (PBCA) perturbs redox homeostasis in human epithelial cells, leading to divergent effects on autophagy depending on the concentration used. Specifically, low concentrations of PBCA promoted functional autophagy, while high concentrations inhibited autophagy. The autophagy-modifying effect of PBCA depended on the severity of redox perturbation and involved activation of MAP kinase signaling cascades.
Article
Biology
Magali Humbert, Maria Moran, Patricia de la Cruz-Ojeda, Jordi Muntane, Tabea Wiedmer, Nadezda Apostolova, Sharon L. McKenna, Guillermo Velasco, Walter Balduini, Leopold Eckhart, Bassam Janji, Belem Sampaio-Marques, Paula Ludovico, Eva Zerovnik, Rupert Langer, Aurel Perren, Nikolai Engedal, Mario P. Tschan
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Biology
Morten Luhr, Frank Saetre, Nikolai Engedal
Article
Biology
Paula Szalai, Nikolai Engedal