Unprecedented Rates and Efficiencies Revealed for New Natural Split Inteins from Metagenomic Sources

Kinetic Control of One-Pot Trans-Splicing Reactions by Using a Wild-Type and Designed Split Intein

(2011) Neel H. Shah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Self-cleaving fusion tags for recombinant protein production

(2011) Yifeng Li   BIOTECHNOLOGY LETTERS

Site-specific modification of ED-B-targeting antibody using intein-fusion technology

(2011) Sina Möhlmann et al.   BMC BIOTECHNOLOGY

Directed Evolution of a Small-Molecule-Triggered Intein with Improved Splicing Properties in Mammalian Cells

(2011) Sun H. Peck et al.   CHEMISTRY & BIOLOGY

Highly Efficient and More Generalcis- andtrans-Splicing Inteins through Sequential Directed Evolution

(2011) Julia H. Appleby-Tagoe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage

(2011) Wei Lu et al.   JOURNAL OF CHROMATOGRAPHY A

Inteins, valuable genetic elements in molecular biology and biotechnology

(2010) Skander Elleuche et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Protein trans-splicing and its use in structural biology: opportunities and limitations

(2010) Gerrit Volkmann et al.   Molecular BioSystems

The potential role of self-cleaving purification tags in commercial-scale processes

(2010) Baley A. Fong et al.   TRENDS IN BIOTECHNOLOGY

Protein splicing: A versatile tool for drug discovery☆

(2009) Manoj Cheriyan et al.   ADVANCED DRUG DELIVERY REVIEWS

Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by ProteinTrans-Splicing Using Only One Robust DnaE Intein

(2009) Alena E. L. Busche et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Split Inteins as Versatile Tools for Protein Semisynthesis

(2009) Henning D. Mootz   CHEMBIOCHEM

The naturally splitNpuDnaE intein exhibits an extraordinarily high rate in the proteintrans-splicing reaction

(2009) Joachim Zettler et al.   FEBS LETTERS

Temperature-Sensitive Mutations Made Easy: Generating Conditional Mutations by Using Temperature-Sensitive Inteins That Function Within Different Temperature Ranges

(2009) G. Tan et al.   GENETICS

Novel Split Intein fortrans-Splicing Synthetic Peptide onto C Terminus of Protein

(2009) Julia H. Appleby et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Splicing of the Mycobacteriophage Bethlehem DnaB Intein

(2009) Kazuo Tori et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Selection and Structure of Hyperactive Inteins: Peripheral Changes Relayed to the Catalytic Center

(2009) Kaori Hiraga et al.   JOURNAL OF MOLECULAR BIOLOGY

Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family

(2009) Bareket Dassa et al.   NUCLEIC ACIDS RESEARCH

Traceless protein splicing utilizing evolved split inteins

(2009) S. W. Lockless et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modulation of intein activity by its neighboring extein substrates

(2009) G. Amitai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Controllable protein cleavages through intein fragment complementation

(2009) Gerrit Volkmann et al.   PROTEIN SCIENCE

Site-Specific Chemical Modification of Proteins with a Prelabelled Cysteine Tag Using the Artificially Split Mxe GyrA Intein

(2008) Thomas Kurpiers et al.   CHEMBIOCHEM