Review
Biochemistry & Molecular Biology
Umut Toprak, Cansu Dogan, Dwayne Hegedus
Summary: This review focuses on the role of two Ca2+ channel receptors in insect cells, IP3R and RyR, which share similarities in structure and function but differ in phylogenetic origins. Both receptors have great potential as target sites in pest control, with RyRs currently being targeted by commercial insecticides.
Article
Computer Science, Artificial Intelligence
Randall Clark, Lawson Fuller, Jason A. Platt, Henry D. I. Abarbanel
Summary: This article introduces how to use methods from nonlinear dynamics and interpolation techniques from applied mathematics to construct discrete time dynamical rules that forecast observed neuron properties accurately, which is crucial for the development of neural network models and biological networks.
NEURAL COMPUTATION
(2022)
Article
Pharmacology & Pharmacy
Andrea Spina, Andrea Guidarelli, Mara Fiorani, Ersilia Varone, Alessia Catalani, Ester Zito, Orazio Cantoni
Summary: Arsenite triggers endoplasmic reticulum stress and increases ERO1 alpha levels, leading to the formation of mitochondrial superoxide. Inhibiting ERO1 alpha prevents the accumulation of Ca2+ in mitochondria and the formation of superoxide.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Laura Marongiu, Francesca Mingozzi, Clara Cigni, Roberta Marzi, Marco Di Gioia, Massimiliano Garre, Dario Parazzoli, Laura Sironi, Maddalena Collini, Reiko Sakaguchi, Takashi Morii, Mariacristina Crosti, Monica Moro, Stephane Schurmans, Tiziano Catelani, Rany Rotem, Miriam Colombo, Stephen Shears, Davide Prosperi, Ivan Zanoni, Francesca Granucci
Summary: Recognition of LPS by DCs involves the receptor complex CD14 and TLR4, and subsequent Ca2+ mobilization and NFAT activation require IP3R3 and ITPKB. Targeting ITPKB may offer a promising approach for anti-inflammatory therapies aimed at inhibiting specific DC functions.
Review
Pathology
Sun-Ji Park, Chuang Li, Ying Maggie Chen
Summary: Maintaining Ca2+ homeostasis is crucial for cellular function and survival, with the endoplasmic reticulum acting as the major intracellular Ca2+ store. Dysregulation of ER Ca2+ homeostasis is associated with various diseases, particularly in kidney diseases, highlighting the importance of ER Ca2+ channels, pumps, and binding proteins. Despite advancements in understanding, treatment for ER Ca2+ imbalance in kidney diseases remains elusive.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Nagendra Babu Thillaiappan, Holly A. Smith, Peace Atakpa-Adaji, Colin W. Taylor
Summary: The study demonstrates that the KRas-induced KRAP protein tethers a subset of IP3 receptors to actin, enabling them to evoke calcium signals. Loss of KRAP results in the disappearance of Ca2+ puffs and a global response to increased cytosolic Ca2+ concentration from more intense stimulation.
NATURE COMMUNICATIONS
(2021)
Review
Neurosciences
Pragnya Chakraborty, Gaiti Hasan
Summary: Key components of endoplasmic reticulum (ER) Ca2+ release and store-operated Ca2+ entry (SOCE) are expressed in all metazoan cells. This review presents evidence on how these two mechanisms impact neuronal physiology and their interaction with ion channels in neurons. The careful regulation of neural SOCE is essential due to the detrimental effects of Ca2+ imbalance on neurons. The study also discusses different mechanisms of SOCE regulation in Drosophila and mammalian neurons, and their significance in human neurodegenerative diseases.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Cell Biology
Ana M. Rossi, Andrew M. Riley, Genevieve Dupont, Taufiq Rahman, Barry V. L. Potter, Colin W. Taylor
Summary: Inositol 1,4,5-trisphosphate receptors (IP(3)Rs) are intracellular Ca2+ channels linking extracellular stimuli to Ca2+ signals. Research shows that quantal responses to low IP3 concentrations do not require heterogeneous Ca2+ stores, and IP(3)Rs exhibit incremental responses to IP3 stimulation. This suggests that Ca2+ signals evoked by IP3 pulses arise from rapid activation and inactivation of a small fraction of IP(3)Rs.
Article
Multidisciplinary Sciences
Guizhen Fan, Mariah R. Baker, Lara E. Terry, Vikas Arige, Muyuan Chen, Alexander B. Seryshev, Matthew L. Baker, Steven J. Ludtke, David Yule, Irina I. Serysheva
Summary: In this study, the cryo-EM structures of IP(3)R1 were determined, providing atomic details of IP3, Ca2+ and ATP binding. The authors identified the molecular motions of key domains and sidechains that regulate ligand binding and gating behavior of IP3R channels.
NATURE COMMUNICATIONS
(2022)
Article
Biophysics
Victor Nicolai Friedhoff, Benjamin Lindner, Martin Falcke
Summary: This article investigates the randomness and properties of Ca2+ signaling in cells. The researchers found that the generation of Ca2+ spikes follows certain patterns and can be described as a process from one state to another. They proposed a theoretical model to explain the differences in spike behavior between different cell types and stimulating agonists, as well as the variability observed in experiments.
BIOPHYSICAL JOURNAL
(2023)
Review
Cell Biology
Jens Loncke, Allen Kaasik, Ilya Bezprozvanny, Jan B. Parys, Martijn Kerkhofs, Geert Bultynck
Summary: Organelles cooperate through membrane contact sites to regulate cellular homeostasis and function. Particularly, the communication between the endoplasmic reticulum and mitochondria enables efficient transmission of Ca2+ signals, tightly controlling mitochondrial functions at a microdomain level.
TRENDS IN CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kyle E. Harvey, Shiqi Tang, Emily K. LaVigne, Evan P. S. Pratt, Gregory H. Hockerman
Summary: In this study, the researchers found that RyR2 is required for the regulation of insulin content and glucose-stimulated insulin secretion in INS-1 cells, partly through its interaction with IRBIT. The experiments showed that the absence of RyR2 led to a decrease in store-operated Ca2+ entry and PLC activity, as well as an increase in cellular PIP2 levels. Furthermore, RyR2 was found to regulate beta-cell electrical activity by controlling Ca-v current density and SK channel activation.
Article
Biochemistry & Molecular Biology
Fan Yang, Hongwei Ma, Michael R. Butler, Xi-Qin Ding
Summary: This study investigated how preserving ER Ca2+ stores leads to cone protection through cone-specific deletion of IP(3)R1, demonstrating that the absence of IP(3)R1 improves protein localization and reduces ER stress and cone death. The findings highlight the importance of ER Ca2+ stores in ER proteostasis and protein trafficking in photoreceptors, supporting the potential of IP(3)R1 as a critical mediator in cone protection.
Article
Multidisciplinary Sciences
Roberto Ornelas-Guevara, Diana Gil, Valerie Voorsluijs, Genevieve Dupont
Summary: Inositol 1,4,5-trisphosphate (IP3) plays a key role in calcium signaling. Previous studies overestimated the diffusion coefficient of IP3 in vivo, but new computational analysis shows that the effective diffusion coefficient is close to 100 μm(2)s(-1), significantly lower than previous estimations. This moderate reduction can be explained by the buffering effect of non-fully bound inactive IP3 receptors.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Orazio Cantoni, Ester Zito, Mara Fiorani, Andrea Guidarelli
Summary: Arsenite, an important carcinogen and toxic compound, exerts its harmful effects mainly by affecting the production of mitochondrial ROS. The interaction between endoplasmic reticulum and mitochondria, as well as the potential regulation by the ER stress response, may play a crucial role in mediating these effects.
SEMINARS IN CANCER BIOLOGY
(2021)
