Article
Biochemistry & Molecular Biology
Maria Kornienko, Dmitry Bespiatykh, Maja Malakhova, Roman Gorodnichev, Nikita Kuptsov, Egor Shitikov
Summary: Phage therapy is a hopeful approach to combat multidrug-resistant bacteria. However, it requires the isolation of new phages or the search for suitable phages in existing libraries. This study proposes a simple PCR method to differentiate between two families of Staphylococcus phages and eleven genera of Klebsiella phages, allowing for rapid screening and identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Youssif M. Ali, Nicholas J. Lynch, Priyanka Khatri, Ifeoluwa E. Bamigbola, Andrew C. Y. Chan, Munehisa Yabuki, Gregory A. Demopulos, Jonathan L. Heeney, Sumita Pai, Helen Baxendale, Wilhelm J. Schwaeble
Summary: A high incidence of secondary Klebsiella pneumoniae and Staphylococcus aureus infection was observed in patients with severe COVID-19. The study suggests that the consumption of complement compromises serum bactericidal activity and predisposes patients to opportunistic infections.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Duong Bich Thuy, James Campbell, Cao Thu Thuy, Nguyen Van Minh Hoang, Phat Voong Vinh, To Nguyen Thi Nguyen, Chau Nguyen Ngoc Minh, Duy Thanh Pham, Maia A. Rabaa, Nguyen Phu Huong Lan, Nguyen Van Hao, Guy E. Thwaites, C. Louise Thwaites, Stephen Baker, Nguyen Van Vinh Chau, Hao Chung The
Summary: This study reveals that preexisting colonization with Staphylococcus aureus or Klebsiella pneumoniae increases the risk of infection in intensive care patients, with infecting isolates often having identical sequence types as colonizing organisms. Multidrug-resistant S. aureus ST188 was found to be the predominant agent of colonization and infection, while infections by K. pneumoniae were characterized by limited antimicrobial resistance but extensive virulence genes. These findings provide insight into the link between bacterial colonization and infection, potentially aiding in the development of evidence-based approaches to prevent and treat infections in high-risk intensive care patients in low-resource settings.
MICROBIAL GENOMICS
(2021)
Article
Immunology
Hatem A. Abuelizz, Mohamed Marzouk, Ahmed Bakhiet, Marwa M. Abdel-Aziz, Essam Ezzeldin, Harunor Rashid, Rashad Al-Salahi
Summary: The research identified benzoquinazolines as potential antimicrobial agents against resistant microbial strains, with benzoquinazoline 3 showing stronger effects. These findings may inform future inhibitor discoveries targeting penicillin-binding proteins.
MICROBIAL PATHOGENESIS
(2021)
Article
Medical Laboratory Technology
Nilgun Kansak, Neslihan Arici, Yasemin Uzunoner, Riza Adaleti, Sebahat Aksaray, Nevriye Gonullu
Summary: The colistin broth disk elution (CBDE) method recommended by CLSI showed 100% concordance with the reference broth microdilution (rBMD) method in detecting colistin resistance. Therefore, the CBDE method can be considered as a satisfactory alternative for routine laboratory work in detecting colistin resistance.
CLINICAL LABORATORY
(2023)
Article
Medicine, General & Internal
Christopher Duplessis, Thomas C. Luke, Chase Watters, Yolanda Alcorta, Biswajit Biswas
Summary: The study attempted to recover phages by collecting skin swab samples from multiple anatomical sites, but the results showed insufficient biomass for phage recovery. This provides useful information for future research and suggests optimizing biomass recovery for phages.
Review
Medicine, Research & Experimental
Neal Patel, Justin Gold, Nolan J. Brown, Mickey Abraham, Ryan S. Beyer, Chenyi Yang, Joshua R. Moore, Scott T. Saunders, Shane Shahrestani, Julian Gendreau, Antonios Mammis
Summary: A systematic review found that preoperative S aureus swabbing and decolonization can significantly reduce the risk of infection in neuromodulation procedures, suggesting that it may be an effective tool for reducing surgical site infection in neuromodulation.
Article
Biotechnology & Applied Microbiology
Nianqing Kong, Shenghuang Shu, Cangyun Zhang, Chuyi Li, Yongwen Luo, Shuying Fang, Shuilian Bi
Summary: A new typing method called intergenic region polymorphism analysis (IRPA) was developed and validated to distinguish between Klebsiella pneumoniae strains. This method was found to have higher discriminatory power and simpler band profile interpretation compared to the multiple-locus variable-number tandem repeat analysis (MLVA) method. It is a rapid, simple, and high-resolution technique for molecular typing of K. pneumoniae.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Immunology
Shio-Shin Jean, Wen-Sen Lee, Wen-Chien Ko, Po-Ren Hsueh
Summary: Ceftaroline demonstrates unique activity against MRSA in Taiwan and shows good in vitro susceptibility against other Gram-positive bacteria and pathogens like H. influenzae. However, some K. pneumoniae strains exhibit non-susceptibility to ceftaroline.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Biology
Teba A. Aziz, Inam J. Lafta
Summary: This study validated the use of multiplex PCR as a specific, sensitive, rapid, accurate, and cost-effective molecular diagnostic method for identifying and differentiating three clinically important bacteria associated with sheep respiratory tract infections. The technique efficiently detected combinations of two or three organisms in the same PCR reaction tube, providing valuable support for disease control and treatment, as well as improving the economy and wellbeing of sheep owners.
Article
Infectious Diseases
Massa Dit Achille Bonko, Palpouguini Lompo, Marc Christian Tahita, Francois Kiemde, Ibrahima Karama, Athanase M. Some, Petra F. Mens, Sandra Menting, Halidou Tinto, Henk D. F. H. Schallig
Summary: The study found bacterial colonization in 154 children, with 96.1% carrying S. aureus, 3.2% carrying S. pneumoniae, and 0.6% carrying both. S. aureus showed high resistance to penicillin (96.0%), while S. pneumoniae was highly resistant to tetracycline and trimethoprim-sulfamethoxazole, and moderately resistant to penicillin. Furthermore, 4.0% of identified S. aureus were methicillin resistant.
Article
Microbiology
Cecile Emeraud, Samy Figueiredo, Remy A. Bonnin, Mouna Khecharem, Souad Ouzani, Pierre-Etienne Leblanc, Agnes B. Jousset, Nicolas Fortineau, Jacques Duranteau, Laurent Dortet
Summary: Infections caused by ESBL-KP are on the rise globally and often lead to outbreaks in ICUs. Despite enhanced hygiene measures and prevention controls during the COVID-19 pandemic, an ESBL-producing K. pneumoniae isolate still caused a nosocomial outbreak in an ICU dedicated to COVID-19 patients.
Article
Agriculture, Multidisciplinary
Claudious Gufe, Tanaka Nicola Mugabe, Zakio Makuvara, Jerikias Marumure, Mbonjani Benard
Summary: This study assessed the antibacterial activity of Ximenia caffra Sond. Myrothamnus flabellifolius Welw. Allium sativum L, and Cinnamomum verum J.Presl extracts against multidrug-resistant mastitogenic Staphylococcus aureus and Klebsiella pneumoniae. The results showed that acetone and methanol extracts of Ximenia caffra and Myrothamnus flabellifolius exhibited significant antimicrobial activity, and when combined with Gentamycin and Lincomycin, the inhibition zones increased. The study concluded that these plant extracts are important in traditional veterinary and healthcare practices.
COGENT FOOD & AGRICULTURE
(2023)
Article
Food Science & Technology
Mengyuan Qi, Ruoqing Zhao, Qingyan Liu, Haiyang Yan, Yan Zhang, Shiyi Wang, Yuan Yuan
Summary: The inhibitory effects of three types of electric fields on Staphylococcus aureus were investigated, with prick electrostatic field (HVPEF) showing the most sensitivity and effectiveness in damaging cell membrane and increasing cell permeability. This technology has potential applications in non-thermal food sterilization.
Article
Microbiology
Ana G. Jop Vidal, Babek Alibayov, I. J. Frame, Landon Murin, Aidan Creel, Dongping Hu, Xueqing Wu, Jorge E. Vidal
Summary: Streptococcus pneumoniae kills Staphylococcus aureus through a contact-dependent mechanism that is facilitated by cations, including iron. Some strains of S. pneumoniae have acquired the Mega gene to resist macrolide antibiotics. Our study found that disrupting iron acquisition or using Mega to resist antibiotics did not inhibit the killing mechanism.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Materials Science, Textiles
Sabina Fijan, Dunja Pahor, Sonja Sostar Turk
TEXTILE RESEARCH JOURNAL
(2017)
Article
Environmental Sciences
Sabina Fijan, Dunja Sulc, Andrej Steyer
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2018)
Article
Environmental Sciences
Sabina Fijan, Anita Frauwallner, Laszlo Varga, Tomaz Langerholc, Irena Rogelj, Mateja Lorber, Peter Lewis, Petra Povalej Brzan
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2019)
Review
Biotechnology & Applied Microbiology
Sabina Fijan, Anita Frauwallner, Tomaz Langerholc, Bojan Krebs, Jessica A. ter Haar (nee Younes), Adolf Heschl, Dusanka Micetic Turk, Irena Rogelj
BIOMED RESEARCH INTERNATIONAL
(2019)
Review
Nutrition & Dietetics
Jann P. Foster, Hannah G. Dahlen, Sabina Fijan, Nadia Badawi, Virginia Schmied, Charlene Thornton, Caroline Smith, Kim Psaila
Summary: Probiotics show promising effects in preventing and treating infant regurgitation by reducing episodes of regurgitation and increasing the number of stools per day. However, there was no significant difference in body weight between the two groups. Probiotics could be a safe and cost-effective health strategy for both women and their babies.
MATERNAL AND CHILD NUTRITION
(2022)
Article
Public, Environmental & Occupational Health
Maja Sikic-Pogacar, Dusanka Micetic Turk, Sabina Fijan
Summary: Fermented foods are well-known and consumed in North-eastern Slovenia, with a higher knowledge of fermentation among older individuals. While most participants recognized the role of lactic acid bacteria and yeasts in fermentation, awareness of molds was lower. Only a small percentage of participants had experience with homemade fermented foods, but a majority were aware of the health benefits, primarily in terms of gastrointestinal health and the immune system. The study highlights the need to educate younger generations about the nutritional value and preparation of fermented foods.
Review
Microbiology
Andrej Steyer, Dusanka Micetic-Turk, Sabina Fijan
Summary: This review summarizes the latest scientific evidence on the antiviral properties of probiotics against rotavirus gastroenteric infections in children. The study found that probiotics can shorten the duration of diarrhea by enhancing immune function and modulating intestinal microbiota. However, the effectiveness of probiotics may be influenced by factors such as dosage, duration of treatment, quality, and other factors.
Article
Microbiology
Sabina Fijan, Primoz Kocbek, Andrej Steyer, Polona Maver Vodicar, Maja Strauss
Summary: The skin microbiota plays an important role in protecting the skin, and disruption of this microbiota can lead to skin infections. Probiotics and their metabolites have been found to have antimicrobial effects against skin pathogens. This study evaluated the antimicrobial effects of seven multi-strain dietary supplements and eleven single-strain microbes containing probiotics against 15 clinical wound pathogens. The results showed that the multi-strain dietary supplements were more effective against the wound pathogens compared to single-strain microbes, with lactobacilli-containing supplements and single-strain microbes being the most efficient.
Review
Pharmacology & Pharmacy
Sabina Fijan, Nina Kolc, Metka Hrasovec, Gro Jamtvedt, Maja Sikic Pogacar, Dusanka Micetic Turk, Uros Maver
Summary: This study evaluated the effect of single-strain probiotic lactobacilli on treating children's atopic dermatitis. The results showed that certain strains were more effective in reducing the severity of atopic dermatitis, and treatment time and patient age also played a role.
Editorial Material
Microbiology
Sabina Fijan
Article
Microbiology
Maja Strauss, Dusanka Micetic Turk, Mateja Lorber, Maja Sikic Pogacar, Anton Kozelj, Ksenija Tusek Bunc, Sabina Fijan
Summary: Immunosenescence increases the risk of infections and morbidity in older people. This study found that supplementing with a multi-strain probiotic can shorten the duration of acute upper respiratory tract infections in older individuals and regulate blood cell counts.
Article
Health Care Sciences & Services
Anton Kozelj, Maja Sikic Pogacar, Sabina Fijan, Maja Strauss, Vita Postuvan, Matej Strnad
Summary: The purpose of this study was to determine the stressors and the level of stress experienced by nurses during resuscitation. The most disturbing situations for nurses were resuscitation of young person, failure to establish an intravenous pathway, chaotic situation during resuscitation, and making decision about termination of resuscitation.
Review
Health Care Sciences & Services
Klavdija Cucek Trifkovic, Dusanka Micetic-Turk, Sergej Kmetec, Maja Strauss, Hannah G. Dahlen, Jann P. Foster, Sabina Fijan
Summary: The mother-infant bond is influenced by maternal mental health and infant behaviors, and one of the potential ways to improve maternal mental health is through probiotic supplementation. Probiotics can manipulate the gut-brain axis and indirectly improve maternal mood by modulating the infant microbiome. However, strain-specific effectiveness should be considered, and further well-designed clinical studies are needed.
Review
Health Care Sciences & Services
Maja Strauss, Dusanka Micetic-Turk, Maja Sikic Pogacar, Sabina Fijan
Summary: This systematic review aimed to demonstrate the indirect influence of probiotics on acute upper respiratory tract infections in older people by regulating the immune system. Some probiotic strains were found to be more effective than placebo in reducing the incidence of such infections, but not all strains were efficient, highlighting the need for more high-quality large-scale studies.
Meeting Abstract
Gastroenterology & Hepatology
Sabina Fijan, Anita Frauwallner, Tomaz Langerholz, Bojan Krebs, Jessica Ter Haar, Adolf Heschl, Dusanka Micetic Turk, Irena Rogelj
JOURNAL OF CLINICAL GASTROENTEROLOGY
(2020)