Article
Biology
Min Lu, Yongli Li, Mei X. Wu
Summary: Multidrug-resistant bacteria pose a significant threat and are depleting treatment options. This study explores the combination of blue-laser and thymol to eradicate these bacteria, successfully preventing systematic dissemination in mice. The strategy leverages the unique properties of bacteria to transform harmless thymol into a powerful sterilizer when activated by blue-laser excitation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Microbiology
Laura J. Dunphy, Kassandra L. Grimes, Nishikant Wase, Glynis L. Kolling, Jason A. Papin
Summary: In a synthetic cystic fibrosis media, distinct species-specific metabolic signatures were revealed for Pseudomonas aeruginosa and Staphylococcus aureus, indicating potential nutrient competition's impact on community dynamics. Both bacteria consumed similar nutrients, suggesting metabolic competition's role in coinfection dynamics. Furthermore, unique metabolites produced by each species may serve as species-specific biomarkers for cystic fibrosis lung infections.
Article
Biophysics
Wing-Hin Lee, Ramin Rohanizadeh, Ching-Yee Loo
Summary: This study developed a novel bioactive bone substitute with improved anti-biofilm activity by functionalizing with curcumin, which effectively inhibits biofilm formation and controls viable planktonic cells. Curcumin was successfully incorporated onto HA and sustainably released over 14 days, showing stronger anti-biofilm effect against Staphylococcus aureus. The curcumin-loaded HA composite could be an alternative antimicrobial agent to control the risk of infections in postsurgical implants.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Critical Care Medicine
Anthony J. Fischer, Sachinkumar B. Singh, Mason M. LaMarche, Lucas J. Maakestad, Zoe E. Kienenberger, Tahuanty A. Pena, David A. Stoltz, Dominique H. Limoli
Summary: Staphylococcus aureus and Pseudomonas aeruginosa are both abundant in sputum cultures of cystic fibrosis patients. Contrary to common assumption, there is no rapid replacement of S. aureus by P. aeruginosa. Many CF patients have long-term coinfection with these organisms.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Review
Immunology
Shalini Shah, Rachel A. F. Wozniak
Summary: Bacterial keratitis is a leading cause of vision loss globally. Immediate use of broad-spectrum antibiotics is crucial to adequately treat this aggressive disease, but antibiotic resistance is increasingly limiting the effectiveness of conventional therapies. This review discusses the pathogenesis of Staphylococcus aureus and Pseudomonas aeruginosa, two common causes of bacterial keratitis, and explores novel therapies under development.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Wusheng Deng, Yanmei Lei, Xiujia Tang, Dingbin Li, Jinhua Liang, Jing Luo, Liuyuan Liu, Wenshu Zhang, Liumei Ye, Jinliang Kong, Ke Wang, Zhaoyan Chen
Summary: This study investigated whether DNase I can inhibit the early formation of biofilms in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. The results showed that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting bacterial growth. Therefore, DNase I has therapeutic potential against empyema biofilms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mahere Momenijavid, Himen Salimizand, Aazam Korani, Omid Dianat, Bijan Nouri, Rashid Ramazanzadeh, Amjad Ahmadi, Jino Rostamipour, Mohammad Rastegar Khosravi
Summary: This study evaluated the effects of calcium hydroxide on the morphology and physicochemical properties of Enterococcus faecalis biofilm. It was found that calcium hydroxide could contribute to a denser biofilm structure with more cavities and a granular surface, resulting in increased biomass, thickness, colony size, and volume of the biofilm.
SCIENTIFIC REPORTS
(2022)
Review
Immunology
Lalitha Biswas, Friedrich Goetz
Summary: Cystic fibrosis is a genetic disorder characterized by recurrent lung infections caused by Staphylococcus aureus and Pseudomonas aeruginosa. The interactions between these pathogens play a significant role in their survival, antibiotic resistance, and disease progression. Understanding these interactions is crucial for the treatment and prevention of cystic fibrosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Materials Science, Paper & Wood
Nguyen Thi Thuy Linh, Tran Chau Diep, Tran Tuong Vy, Nguyen Minh Dat, Dinh Ngoc Trinh, Doan Ba Thinh, Nguyen Duc Viet, Nguyen Duy Hai, Le Minh Huong, Ninh Thi Tinh, Mai Thanh Phong, Nguyen Huu Hieu
Summary: In this study, a cotton fabric with immobilized silver nanoparticles on reduced graphene oxide (Ag/rGO/cotton) was successfully prepared using dip-coating and supplemented with vitamin C as a reducing agent. The modified cotton exhibited uniform distribution of silver nanoparticles on graphene oxide sheets, as well as high antibacterial activity and effective hydrophobicity.
Article
Biology
Camryn Pajon, Marla C. Fortoul, Gabriela Diaz-Tang, Estefania Marin Meneses, Ariane R. Kalifa, Elinor Sevy, Taniya Mariah, Brandon Toscan, Maili Marcelin, Daniella M. Hernandez, Melissa M. Marzouk, Allison J. Lopatkin, Omar Tonsi Eldakar, Robert P. Smith
Summary: Most bacteria exist and interact within polymicrobial communities, producing unique compounds, increasing virulence, and enhancing antibiotic resistance. This study demonstrates that changes in the growth environment can determine the final population composition of bacteria, highlighting the importance of considering the growth environment in understanding bacterial coexistence. The researchers also found that altering the carbon source in the growth media impacts the absolute growth of bacterial species and their dominance in co-culture. Furthermore, perturbing the spatial structure in the community can affect the relationship between growth and population composition.
Article
Microbiology
Eryn E. Bernardy, Vishnu Raghuram, Joanna B. Goldberg
Summary: The interaction between Staphylococcus aureus and Pseudomonas aeruginosa in the lungs of cystic fibrosis patients is key to understanding the severity of respiratory infections. The survival of S. aureus is significantly higher when coinfected with P. aeruginosa, suggesting that interactions between these two bacteria promote coexistence in the CF lung environment.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Xiao-Mei Yan, Jing Wang, Xiao-Xia Tao, Hong-Bing Jia, Fan-Liang Meng, Hui Yang, Yuan-Hai You, Bo Zheng, Yuan Hu, Xiao-Xia Bu, Jian-Zhong Zhang
Summary: The lsa(E) gene was found in about half of E. faecium isolates from a hospital in Beijing, with 78.3% of carriers belonging to ST78. Under experimental conditions, the lsa(E) gene could be successfully transferred from E. faecium to S. aureus at a low frequency. The transconjugant showed significantly increased resistance, possibly due to carrying multiple antibiotic resistance genes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Sylvie Letoffe, Yongzheng Wu, Sophie E. Darch, Christophe Beloin, Marvin Whiteley, Lhousseine Touqui, Jean-Marc Ghigo
Summary: This study demonstrates that hydrogen cyanide (HCN) released by Pseudomonas aeruginosa inhibits the growth of Staphylococcus aureus, particularly in low-oxygen environments. These findings are significant for understanding infectious diseases like cystic fibrosis in polymicrobial communities.
Article
Microbiology
Ines Reigada, Paola San-Martin-Galindo, Shella Gilbert-Girard, Jacopo Chiaro, Vincenzo Cerullo, Kirsi Savijoki, Tuula A. Nyman, Adyary Fallarero, Ilkka Miettinen
Summary: When two clinically relevant bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, co-culture in a biofilm, P. aeruginosa shows higher motility and lower pigment production, while S. aureus exhibits increased persistence due to lower abundances of proteins related to cell wall biosynthesis and cell division. Both species also display higher antimicrobial tolerance when co-cultured. This study provides insights into the dynamics of both the surfaceome and exoproteome of the dual-species biofilms.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Ecology
Jun-Hong Ch'ng, Mugil Muthu, Kelvin K. L. Chong, Jun Jie Wong, Casandra A. Z. Tan, Zachary J. S. Koh, Daniel Lopez, Artur Matysik, Zeus J. Nair, Timothy Barkham, Yulan Wang, Kimberly A. Kline
Summary: This study reports the enhanced biofilm biomass resulting from the combination of Staphylococcus aureus and Enterococcus faecalis, which is dependent on the activation of E. faecalis aerobic respiration. S. aureus provides heme to activate E. faecalis respiration, and E. faecalis gelatinase activity facilitates heme extraction. This interspecies interaction and metabolic cross-feeding may explain the frequent co-occurrence of these microbes in biofilm-associated infections.
Article
Environmental Sciences
Sabina Fijan, Dunja Sulc, Andrej Steyer
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2018)
Article
Environmental Sciences
Sabina Fijan, Anita Frauwallner, Laszlo Varga, Tomaz Langerholc, Irena Rogelj, Mateja Lorber, Peter Lewis, Petra Povalej Brzan
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2019)
Review
Biotechnology & Applied Microbiology
Sabina Fijan, Anita Frauwallner, Tomaz Langerholc, Bojan Krebs, Jessica A. ter Haar (nee Younes), Adolf Heschl, Dusanka Micetic Turk, Irena Rogelj
BIOMED RESEARCH INTERNATIONAL
(2019)
Review
Nutrition & Dietetics
Jann P. Foster, Hannah G. Dahlen, Sabina Fijan, Nadia Badawi, Virginia Schmied, Charlene Thornton, Caroline Smith, Kim Psaila
Summary: Probiotics show promising effects in preventing and treating infant regurgitation by reducing episodes of regurgitation and increasing the number of stools per day. However, there was no significant difference in body weight between the two groups. Probiotics could be a safe and cost-effective health strategy for both women and their babies.
MATERNAL AND CHILD NUTRITION
(2022)
Article
Public, Environmental & Occupational Health
Maja Sikic-Pogacar, Dusanka Micetic Turk, Sabina Fijan
Summary: Fermented foods are well-known and consumed in North-eastern Slovenia, with a higher knowledge of fermentation among older individuals. While most participants recognized the role of lactic acid bacteria and yeasts in fermentation, awareness of molds was lower. Only a small percentage of participants had experience with homemade fermented foods, but a majority were aware of the health benefits, primarily in terms of gastrointestinal health and the immune system. The study highlights the need to educate younger generations about the nutritional value and preparation of fermented foods.
Review
Microbiology
Andrej Steyer, Dusanka Micetic-Turk, Sabina Fijan
Summary: This review summarizes the latest scientific evidence on the antiviral properties of probiotics against rotavirus gastroenteric infections in children. The study found that probiotics can shorten the duration of diarrhea by enhancing immune function and modulating intestinal microbiota. However, the effectiveness of probiotics may be influenced by factors such as dosage, duration of treatment, quality, and other factors.
Article
Microbiology
Sabina Fijan, Primoz Kocbek, Andrej Steyer, Polona Maver Vodicar, Maja Strauss
Summary: The skin microbiota plays an important role in protecting the skin, and disruption of this microbiota can lead to skin infections. Probiotics and their metabolites have been found to have antimicrobial effects against skin pathogens. This study evaluated the antimicrobial effects of seven multi-strain dietary supplements and eleven single-strain microbes containing probiotics against 15 clinical wound pathogens. The results showed that the multi-strain dietary supplements were more effective against the wound pathogens compared to single-strain microbes, with lactobacilli-containing supplements and single-strain microbes being the most efficient.
Review
Pharmacology & Pharmacy
Sabina Fijan, Nina Kolc, Metka Hrasovec, Gro Jamtvedt, Maja Sikic Pogacar, Dusanka Micetic Turk, Uros Maver
Summary: This study evaluated the effect of single-strain probiotic lactobacilli on treating children's atopic dermatitis. The results showed that certain strains were more effective in reducing the severity of atopic dermatitis, and treatment time and patient age also played a role.
Editorial Material
Microbiology
Sabina Fijan
Article
Microbiology
Maja Strauss, Dusanka Micetic Turk, Mateja Lorber, Maja Sikic Pogacar, Anton Kozelj, Ksenija Tusek Bunc, Sabina Fijan
Summary: Immunosenescence increases the risk of infections and morbidity in older people. This study found that supplementing with a multi-strain probiotic can shorten the duration of acute upper respiratory tract infections in older individuals and regulate blood cell counts.
Article
Health Care Sciences & Services
Anton Kozelj, Maja Sikic Pogacar, Sabina Fijan, Maja Strauss, Vita Postuvan, Matej Strnad
Summary: The purpose of this study was to determine the stressors and the level of stress experienced by nurses during resuscitation. The most disturbing situations for nurses were resuscitation of young person, failure to establish an intravenous pathway, chaotic situation during resuscitation, and making decision about termination of resuscitation.
Review
Health Care Sciences & Services
Klavdija Cucek Trifkovic, Dusanka Micetic-Turk, Sergej Kmetec, Maja Strauss, Hannah G. Dahlen, Jann P. Foster, Sabina Fijan
Summary: The mother-infant bond is influenced by maternal mental health and infant behaviors, and one of the potential ways to improve maternal mental health is through probiotic supplementation. Probiotics can manipulate the gut-brain axis and indirectly improve maternal mood by modulating the infant microbiome. However, strain-specific effectiveness should be considered, and further well-designed clinical studies are needed.
Review
Health Care Sciences & Services
Maja Strauss, Dusanka Micetic-Turk, Maja Sikic Pogacar, Sabina Fijan
Summary: This systematic review aimed to demonstrate the indirect influence of probiotics on acute upper respiratory tract infections in older people by regulating the immune system. Some probiotic strains were found to be more effective than placebo in reducing the incidence of such infections, but not all strains were efficient, highlighting the need for more high-quality large-scale studies.
Meeting Abstract
Gastroenterology & Hepatology
Sabina Fijan, Anita Frauwallner, Tomaz Langerholz, Bojan Krebs, Jessica Ter Haar, Adolf Heschl, Dusanka Micetic Turk, Irena Rogelj
JOURNAL OF CLINICAL GASTROENTEROLOGY
(2020)