Article
Multidisciplinary Sciences
Jay R. Dave, Sayali S. Chandekar, Shubhanath Behera, Kaushik U. Desai, Pradnya M. Salve, Neha B. Sapkal, Suhas T. Mhaske, Ankush M. Dewle, Parag S. Pokare, Megha Page, Ajay Jog, Pankaj A. Chivte, Rupesh K. Srivastava, Geetanjali B. Tomar
Summary: Aging negatively affects the quantity and quality of mesenchymal stem cells (MSCs) derived from gingival tissues, limiting their therapeutic potential. This study demonstrates that these MSCs exhibit decreased adipogenesis and osteogenesis with age, but their neurogenic potential remains unaffected. Importantly, regardless of donor age, these cells display immunoregulatory and regenerative abilities, making them promising candidates for cell-based therapies in acute inflammatory conditions.
Review
Biochemistry & Molecular Biology
Emily Soice, Jeremiah Johnston
Summary: The need to produce immortal cell lines relevant to food production is a pressing challenge in the field of cellular agriculture, especially for cultured meat production. Previous research on immortal cell lines has focused on toxicity assessment and pharmaceutical applications, but unique attributes are required for cell lines used in cultured meat production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Zhengpeng Wan, Shun Zhang, Amy X. Zhong, Sarah E. Shelton, Marco Campisi, Shriram K. Sundararaman, Giovanni S. Offeddu, Eunkyung Ko, Lina Ibrahim, Mark F. Coughlin, Tiankun Liu, Jing Bai, David A. Barbie, Roger D. Kamm
Summary: By exogenously expressing human telomerase reverse transcriptase (hTERT), human umbilical vein endothelial cells (HUVECs) and lung fibroblasts (FBs) were successfully immortalized, providing stable cell sources for functional microvascular network formation in vitro. It was observed that immortalized FBs expressing Thy1 could interact with immortalized HUVECs to form functional microvascular networks, while Thy1 negative FBs were found to physically block microvessel openings.
Article
Biochemistry & Molecular Biology
Aurelie Dos Santos, Ning Lyu, Alis Balayan, Rob Knight, Katherine Sun Zhuo, Yuzhao Sun, Jianjiang Xu, Martha L. Funderburgh, James L. Funderburgh, Sophie X. Deng
Summary: This study successfully established immortalized CSSC lines by overexpressing SV40T, which can sustain proliferation while retaining similar characteristics and anti-inflammatory properties. These immortalized CSSCs provide a potential large-scale source for regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Floris J. M. Roos, Haoyu Wu, Jorke Willemse, Ruby Lieshout, Laura A. Munoz Albarinos, Yik-Yang Kan, Jan-Werner Poley, Marco J. Bruno, Jeroen de Jonge, Richard Bartfai, Hendrik Marks, Jan N. M. IJzermans, Monique M. A. Verstegen, Luc J. W. van der Laan
Summary: This study demonstrates the successful initiation of cholangiocyte organoids from bile obtained through various methods, showing similar characteristics to in vivo cholangiocytes in morphology and gene expression. The functionality of organoid-initiating cells in bile has been validated, providing a convenient source for regenerative medicine.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Eun-Jung Sohn, Yun-Kyeong Nam, Hwan-Tae Park
Summary: The study revealed differential expression of miRNAs in cAMP-treated Schwann cells, with upregulation of miRNA363-5p targeting P2X purinoceptor 4 (P2RX4). The study suggests a double-negative feedback loop between miRNA363-5p and P2RX4 contributes to Schwann cell dedifferentiation and migration after nerve injury. Additionally, a P2RX4 antagonist was found to counteract myelin degradation after nerve injury, further increasing the levels of miRNA363-5p.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Ezgi Akidil, Manuel Albanese, Alexander Buschle, Adrian Ruhle, Dagmar Pich, Oliver T. Keppler, Wolfgang Hammerschmidt
Summary: Efficient genetic manipulation in resting human B cells has been achieved using Cas9 ribonucleoprotein complexes, followed by EBV infection or culture on CD40 ligand feeder cells. This approach enables specific gene targeting in quiescent human B cells, supporting basic research and immunotherapeutic strategies.
Article
Cell Biology
Yingai Shi, Guihua Liu, Rongpei Wu, David L. Mack, Xiuzhi Susan Sun, Joshua Maxwell, Xuan Guan, Anthony Atala, Yuanyuan Zhang
Summary: This study highlights the regenerative significance of telomerase activity in human primary USCs, showing that telomerase-positive USCs have a robust regenerative potential in cell proliferation and multipotent differentiation capacity. The presence of telomerase activity is associated with maintaining cell surface marker expression, chromosomal stability, and in vivo tumorigenic transformation in USCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, General & Internal
Aishwarya Balasankar, Shu-Yi Claire Chan, Venkata Pakala Sudheer Babu, Gary Yam, Goh Bee Tin, Shweta Singhal
Summary: Dental pulp stem cells (DPSCs) derived from the neural crest are easily accessible MSCs that have potential for retinal therapy. This study shows that DPSCs maintain their mesenchymal phenotype and upregulate retinal markers under differentiation conditions. However, current markers are inadequate for isolating neural crest cells in DPSCs, highlighting the need for more specific markers.
FRONTIERS IN MEDICINE
(2022)
Article
Oncology
Giacomo Domenici, Rodrigo Eduardo, Helena Castillo-Ecija, Gorka Orive, Angel Montero Carcaboso, Catarina Brito
Summary: Ewing's Sarcoma (ES) is the second most common malignant bone tumor in children and young adults, with limited treatment options. To study the disease and develop new therapies, experimental models are crucial. The authors developed a 3D cell culture method using PDX-derived ES cells, which maintained cell characteristics and could be used for drug sensitivity assays.
Article
Immunology
Chiara Rancan, Marcel Arias-Badia, Pranay Dogra, Brandon Chen, Dvir Aran, Hai Yang, Diamond Luong, Arielle Ilano, Jacky Li, Hewitt Chang, Serena S. Kwek, Li Zhang, Lewis L. Lanier, Maxwell V. V. Meng, Donna L. Farber, Lawrence Fong
Summary: V delta 2(-) gamma delta T cells infiltrate kidney cancers and phenotypically resemble exhausted T cells, but still maintain cytolytic function and can be used to predict response to immune checkpoint inhibition.
Article
Neurosciences
Shuangchan Wu, Wensheng Lin
Summary: Endoplasmic reticulum associated degradation (ERAD) is responsible for recognizing and degrading unfolded or misfolded proteins in the ER. Sel1L is essential for the ERAD activity and its knockout in mature myelinating cells caused myelin abnormalities in the adult CNS and PNS. The knockout impaired ERAD, leading to ER stress and UPR activation, and suppressed myelin protein translation, resulting in myelin thinning and demyelination in the adult CNS and PNS.
Article
Immunology
Felix Pfister, Jan Doerrie, Niels Schaft, Vera Buchele, Harald Unterweger, Lucas R. Carnell, Patrick Schreier, Rene Stein, Marketa Kubankova, Jochen Guck, Holger Hackstein, Christoph Alexiou, Christina Janko
Summary: The influence of superparamagnetic iron oxide nanoparticles (SPIONs) on primary human T cells and their potential for magnetically targeted adoptive T cell therapy were investigated in this study. Results showed that SPION-loading did not affect the mechanics and functionality of T cells, indicating its potential for magnetically enriching T cells in solid tumors.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
J. Pedro Loureiro, Mariana S. Cruz, Ana P. Cardoso, Maria J. Oliveira, M. Fatima Macedo
Summary: This study investigates the impact of iNKT cells on the survival and polarization of macrophages, showing that iNKT cells activate macrophages through CD40-CD40L co-stimulation and exhibit cytotoxicity towards M1-like macrophages.
Article
Biochemistry & Molecular Biology
Zhong Huang, Rebecca Powell, Svenja Kankowski, James B. Phillips, Kirsten Haastert-Talini
Summary: Researchers have successfully generated Schwann cells from human induced pluripotent stem cells by modifying existing protocols, resulting in sufficient cell numbers. They also found that specific coating and glucose levels are crucial for improving the differentiation process and increasing the yield of viable cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Martin K. R. Svacina, Anika Meissner, Finja Schweitzer, Anne Ladwig, Alina Sprenger-Svacina, Ines Klein, Hauke Wuestenberg, Felix Kohle, Christian Schneider, Nicolai B. Grether, Gilbert Wunderlich, Gereon R. Fink, Florian Klein, Veronica Di Cristanziano, Helmar C. Lehmann
Summary: The study found that IVIg does not impair the antibody response to COVID-19 mRNA vaccine and does not significantly alter serum anti-SARS-CoV-2 IgG titers.
EUROPEAN JOURNAL OF NEUROLOGY
(2022)
Article
Clinical Neurology
Samuel Arends, Judith Drenthen, Peter Y. K. van den Bergh, Robert D. M. Hadden, Nortina Shahrizaila, Mazen M. Dimachkie, Gerardo Gutierrez Gutierrez, Hans Katzberg, Lynette Kiers, Helmar C. Lehmann, Yann Pereon, Ricardo C. Reisin, Antonino Uncini, Camiel Verhamme, Wagar Waheed, David R. Cornblath, Bart C. Jacobs
Summary: This study investigated how neuromuscular EDx experts approach key aspects of GBS subtyping in practice and compared previously published EDx criteria for GBS. The study found extensive variability in the practical use of EDx criteria, potentially reducing the reproducibility of GBS subtyping.
JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM
(2022)
Article
Clinical Neurology
Helmar C. Lehmann
Summary: Long-COVID syndrome refers to symptoms that occur after SARS-CoV-2 infections and persist for several weeks. This review article summarizes the data on neuromuscular manifestations of long-COVID and points out the limited evidence available so far.
Article
Clinical Neurology
Nicolai B. Grether, Felix Napravnik, Thomas Imhof, Reinhold P. Linke, Jan H. Brasen, Jessica Schmitz, Maike Dohrn, Christian Schneider, Martin K. R. Svacina, Jorg Stetefeld, Manuel Koch, Helmar C. Lehmann
Summary: The objective of this study was to assess the amyloidogenic potential of variants of uncertain significance in vitro. Results showed that transthyretin-Ala65Val had significantly higher amyloidogenic potential compared to wild-type transthyretin, while the other eight variants did not show increased amyloidogenic potential. The combination of three assays and histopathology confirmed the amyloidogenicity of transthyretin-Ala65Val.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2022)
Article
Clinical Neurology
Alina Sprenger-Svacina, Johannes Haensch, Kilian Weiss, Nils Grosse Hokamp, David Maintz, Marc Schlamann, Gereon R. Fink, Natalie Schloss, Kai Laukamp, Gilbert Wunderlich, Helmar C. Lehmann, Thorsten Lichtenstein
Summary: This study evaluated the utility of a multi-parametric MRI protocol to detect loss of spinal motor neurons and subsequent muscle damage in adult SMA patients. The results showed that water T-2 mapping and Dixon-based PDFF estimation can distinguish between SMA patients and controls.
JOURNAL OF NEUROLOGY
(2023)
Article
Clinical Neurology
Anna Lena Fisse, Jeremias Motte, Thomas Grueter, Felix Kohle, Cornelius Kronlage, Jan-Hendrik Stahl, Natalie Winter, Tabea Seeliger, Stefan Gingele, Frauke Stascheit, Benjamin Hotter, Juliane Klehmet, Karsten Kummer, Elena K. Enax-Krumova, Dietrich Sturm, Thomas Skripuletz, Jens Schmidt, Min-Suk Yoon, Kalliopi Pitarokoili, Helmar C. Lehmann, Alexander Grimm
Summary: This study investigates the current care of patients with immune-mediated polyneuropathies in specialized centers in Germany and provides important epidemiological insights. The findings suggest that the development of specific treatment and follow-up recommendations is necessary to ensure a uniform standard of patient care.
Biographical-Item
Clinical Neurology
Justin C. McArthur, Klaus Toyka, Ahmet Hoke
ANNALS OF NEUROLOGY
(2023)
Article
Clinical Neurology
Martin K. R. Svacina, Alina Sprenger-Svacina, Anastasia Tsakmaklis, Alina M. Rueb, Ines Klein, Hauke Wuestenberg, Gereon R. Fink, Helmar C. Lehmann, Maria J. G. T. Vehreschild, Fedja Farowski
Summary: This study found that the gut microbiome of CIDP patients has increased diversity and enrichment of Firmicutes, which produce short-chain fatty acids. However, intravenous immunoglobulin (IVIg) administration did not have a short-term impact on the gut microbiome in CIDP patients.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Editorial Material
Clinical Neurology
Ahmet Hoke
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2023)
Article
Clinical Neurology
Ye Eun Jeong, Labchan Rajbhandari, Byung Woo Kim, Arun Venkatesan, Ahmet Hoke
Summary: Objective: Neurodegeneration induced by inflammatory stress in multiple sclerosis (MS) leads to long-term neurological disabilities that are not amenable to current immunomodulatory therapies. Methods and Results: This study found that neuronal downregulation of Splicing factor 3b subunit 2 (SF3B2) preserves retinal ganglion cell (RGC) survival and axonal integrity in experimental autoimmune encephalomyelitis (EAE)-induced mice. The downregulation of SF3B2 prevents activation of Sterile Alpha and TIR Motif Containing 1 (Sarm1), a key enzyme that mediates programmed axon degeneration. Interpretation: The downregulation of SF3B2 is a novel potential therapeutic target to prevent secondary neurodegeneration in MS.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2023)
Article
Neurosciences
Martin K. R. Svacina, Anika Meissner, Finja Schweitzer, Alina Sprenger-Svacina, Ines Klein, Hauke Wuestenberg, Felix Kohle, Helene L. Walter, Michael Schroeter, Helmar C. Lehmann
Summary: This exploratory study analyzed the impact of COVID-19 mRNA vaccine on disease activity and IVIg-immunomodulation in CIDP patients. The results showed that except for a significantly lower expression of CD32b on naive B cells after vaccination, there were no significant alterations in immunomarkers for CIDP or IVIg-mediated immunomodulation. This study suggests that COVID-19 mRNA vaccine does not affect immune activity in CIDP and does not alter the immunomodulatory effects of IVIg.
JOURNAL OF NEUROIMMUNE PHARMACOLOGY
(2023)
Review
Clinical Neurology
Susanna B. Park, Aysel Cetinkaya-Fisgin, Andreas A. Argyriou, Ahmet Hoeke, Guido Cavaletti, Paola Alberti
Summary: Multiple mechanisms contribute to chemotherapy-induced peripheral neurotoxicity (CIPN), including axonal degeneration. This review integrates evidence from preclinical and clinical studies to examine the triggers and molecular pathways involved in axonal degeneration in CIPN, with a focus on the SARM1-mediated pathway. Potential clinical markers of axonal dysfunction are discussed, along with potential treatment strategies to intervene in axonal degeneration. Understanding the processes underlying axonal degeneration in CIPN will have broader implications for the development and progression of axonal dysfunction, and may lead to successful interventions for CIPN and other neurodegenerative disorders.
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2023)
Article
Cell Biology
Ines Klein, Jorg Isensee, Martin H. J. Wiesen, Thomas Imhof, Meike K. Wassermann, Carsten Mueller, Tim Hucho, Manuel Koch, Helmar C. Lehmann
Summary: Peripheral neuropathy is a common side effect of cancer treatment with paclitaxel, but the mechanisms of paclitaxel transport into neurons are not well understood. This study identified OATP1A1 and OATP1B2 as the primary neuronal transporters for paclitaxel and suggested that inhibiting these transporters may prevent paclitaxel-induced neuropathy.
Article
Medicine, Research & Experimental
Andres Fuentes-Flores, Cristian Geronimo-Olvera, Karina Girardi, David Necunir-Ibarra, Sandip Kumar Patel, Joanna Bons, Megan C. Wright, Daniel Geschwind, Ahmet Hoke, Jose A. Gomez-Sanchez, Birgit Schilling, Daniela L. Rebolledo, Judith Campisi, Felipe A. Court
Summary: Reparative Schwann cells transition into a senescent phenotype in aging and chronic denervation, leading to inhibition of axonal regeneration. Elimination of senescent cells improves nerve regeneration and functional recovery.
EMBO MOLECULAR MEDICINE
(2023)
Review
Clinical Neurology
Felix Kohle, Marinos C. C. Dalakas, Helmar C. C. Lehmann
Summary: Despite advances in the treatment of CIDP and other AN, many patients remain unsatisfied with available treatments. Repurposing DMTs from other autoimmune conditions, particularly MS and NMOSD, is a promising strategy for establishing new treatment options for AN. B cell-directed and complement-targeting strategies, such as anti-CD20/anti-CD19 agents, Bruton's tyrosine kinase inhibitors, and anti-C5 agents, show promise as therapeutic approaches for AN.
THERAPEUTIC ADVANCES IN NEUROLOGICAL DISORDERS
(2023)