Article
Immunology
Sophie Carina Kunte, Julian A. Marschner, Martin Klaus, Tamisa Honda, Chenyu Li, Manga Motrapu, Christoph Walz, Maria Lucia Angelotti, Giulia Antonelli, Maria Elena Melica, Letizia De Chiara, Roberto Semeraro, Peter J. Nelson, Hans-Joachim Anders
Summary: This study demonstrated through single-cell transcriptome analysis and experiments that NLRP3 inflammasome expression in renal parenchymal cells is low or even nonexistent, and podocytes do not produce IL-1β and IL-18. Therefore, NLRP3-mediated glomerular inflammation is limited to immune cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Yanbing Qiu, Yumei Huang, Meilin Chen, Yuqin Yang, Xiaoxu Li, Wenling Zhang
Summary: NLRP3 inflammasome is an intracellular polyprotein complex that is activated by perceiving different molecular patterns. Intracellular mtDNA and extracellular mtDNA have different functions in activating NLRP3 inflammasome.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Urology & Nephrology
Khurrum Shahzad, Sameen Fatima, Hamzah Khawaja, Ahmed Elwakiel, Ihsan Gadi, Saira Ambreen, Silke Zimmermann, Peter R. Mertens, Ronald Biemann, Berend Isermann
Summary: This study found that activation of the NLRP3 inflammasome in podocytes is both necessary and sufficient for diabetic kidney disease (DKD), indicating that podocytes have immune cell-like functions. Targeting NLRP3 may be a promising therapeutic approach for DKD.
KIDNEY INTERNATIONAL
(2022)
Article
Medicine, Research & Experimental
Junnan Wu, Archana Raman, Nathan J. Coffey, Xin Sheng, Joseph Wahba, Matthew J. Seasock, Ziyuan Ma, Pazit Beckerman, Dorottya Laczko, Matthew B. Palmer, Jeffrey B. Kopp, Jay J. Kuo, Steven S. Pullen, Carine M. Boustany-Kari, Andreas Linkermann, Katalin Susztak
Summary: Coding variants in APOL1, especially G1 and G2, are key factors contributing to excess kidney disease risk in African Americans. The study reveals that expression of G2 APOL1 induces activation of STING and NLRP3 inflammasome in podocytes, leading to kidney dysfunction. Inhibition of NLRP3, GSDMD, and STING shows promising therapeutic potential for APOL1-associated kidney disease.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Antonella La Russa, Danilo Lofaro, Alberto Montesanto, Daniele La Russa, Gianluigi Zaza, Simona Granata, Michele Di Dio, Raffaele Serra, Michele Andreucci, Renzo Bonofiglio, Anna Perri
Summary: In this study, the association of functional variants of NLRP3 pathway-related genes (NLRP3-rs10754558, CARD8-rs2043211) with CKD susceptibility was investigated for the first time. Logistic regression analyses revealed significantly higher frequencies of the G allele of NLRP3 variant (67.3%) and the T allele of CARD8 variant (70.8%) among cases compared to controls (35.9% and 31.2%, respectively). The results suggest that NLRP3 rs10754558 and CARD8 rs2043211 variants may be associated with susceptibility to CKD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Mick Frissen, Lijun Liao, Kai Markus Schneider, Sonja Djudjaj, Johannes Haybaeck, Alexander Wree, Ulrike Rolle-Kampczyk, Martin von Bergen, Eicke Latz, Peter Boor, Christian Trautwein
Summary: NLRP3 plays a different role in acute and chronic cholestatic liver injury compared to kidney injury. Disease progression during chronic cholestasis can be targeted through small molecules, suggesting potential clinical benefits for humans in attenuating liver and kidney injury.
Article
Biochemistry & Molecular Biology
Yung-Ho Hsu, Cai-Mei Zheng, Chu-Lin Chou, Yi-Jie Chen, Yu-Hsuan Lee, Yuh-Feng Lin, Hui-Wen Chiu
Summary: Chronic inflammation and oxidative stress play significant roles in the development and progression of chronic kidney disease (CKD). The activation of the NLRP3 inflammasome and the renal endothelin (ET) system contribute to inflammation and cellular injury in CKD. Inhibiting endothelin-converting enzymes (ECEs) through autophagy activation, and restraining ER stress and the NLRP3 inflammasome could be a novel strategy to delay CKD progression.
Article
Cell Biology
Qi Pang, Peiwen Wang, Yajing Pan, Xingtong Dong, Ting Zhou, Xinyu Song, Aihua Zhang
Summary: This study demonstrates for the first time that Irisin protects the cardiovascular system against vascular calcification in CKD by inducing autophagy and inhibiting VSMC pyroptosis.
CELL DEATH & DISEASE
(2022)
Article
Immunology
Yuhua Shi, Qian Lv, Mengjie Zheng, Hongxiang Sun, Fushan Shi
Summary: INF39 is a specific inhibitor for NLRP3 inflammasome activation, without affecting other inflammasomes. It exerts its anti-inflammatory effect by inhibiting the interaction of NEK7-NLRP3 and other downstream events.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Meng Song, Zijun Chen, Ruian Qiu, Tingwei Zhi, Wenmin Xie, Yingya Zhou, Nachuan Luo, Fuqian Chen, Fang Liu, Chuangpeng Shen, Sheng Lin, Fengxue Zhang, Yong Gao, Changhui Liu
Summary: Excessive accumulation of bile acids (BA) in hepatocytes triggers inflammatory response and recruitment of macrophages, accelerating cholestatic liver injury. This study reveals the importance of crosstalk between hepatocytes and macrophages in the pathogenesis of cholestasis and suggests that blocking this crosstalk by inhibiting NLRP3 inflammasome activation may be a novel therapeutic strategy for cholestasis.
Review
Biochemistry & Molecular Biology
Burak Ibrahim Arioz, Emre Tarakcioglu, Melis Olcum, Sermin Genc
Summary: The NLRP3 inflammasome, as a part of the innate immune system, is associated with various diseases when overactivated; while melatonin, a natural product, can alleviate NLRP3 inflammasome activity by regulating intracellular signaling pathways.
Article
Multidisciplinary Sciences
Inga Hochheiser, Michael Pilsl, Gregor Hagelueken, Jonas Moecking, Michael Marleaux, Rebecca Brinkschulte, Eicke Latz, Christoph Engel, Matthias Geyer
Summary: This study determined the conformational states of human NLRP3 and its interaction with the inhibitor CRID3 through cryo-electron microscopy. The findings provide insights into the regulatory mechanism of NLRP3 and offer potential for the treatment of related diseases.
Review
Immunology
Wei Xiong, Xian-Fang Meng, Chun Zhang
Summary: Metabolic syndrome can lead to metabolic disorders and impair kidney structure and function, while persistent inflammation exacerbates renal damage. Inflammasomes are key in initiating inflammation, and understanding their role in kidney damage caused by metabolic disorders can provide new therapeutic perspectives for kidney diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Han Cheng, Lingling Chen, Minchun Huang, Jin Hou, Zhifeng Chen, Xiaojun Yang
Summary: NLRP3 inflammasome plays a key role in radiation-induced tissue injury, but its potential value is not adequately recognized. Early intervention/prevention strategies targeting NLRP3 inflammasome may help resolve clinical problems caused by radiation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Xiao-fei He, Li-li Li, Wen-biao Xian, Ming-yue Li, Li-ying Zhang, Jing-hui Xu, Zhong Pei, Hai-qing Zheng, Xi-quan Hu
Summary: Neuroinflammation plays a significant role in age-related brain degeneration, especially in conditions such as Alzheimer's disease. Inflammatory bowel diseases (IBDs) can induce neuroinflammation and worsen cognitive impairment in elderly individuals. The NLRP3 inflammasome may contribute to glymphatic dysfunction and cognitive impairment in aging mice with IBD, suggesting that targeting this pathway could have protective effects against colitis-induced neurodegeneration.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Biochemistry & Molecular Biology
Giovanna Butera, Marcello Manfredi, Alessandra Fiore, Jessica Brandi, Raffaella Pacchiana, Veronica De Giorgis, Elettra Barberis, Virginia Vanella, Marilisa Galasso, Maria Teresa Scupoli, Emilio Marengo, Daniela Cecconi, Massimo Donadelli
Summary: The study of the cancer secretome is essential for understanding the role of wild-type p53 in the cancer microenvironment. This research demonstrates the tumor suppressive effects of wild-type p53 on cancer cell secretome in pancreatic ductal adenocarcinoma (PDAC). By using high-resolution SWATH-MS technology, the researchers identified several secreted proteins that are involved in cancer-related processes, with their secretion levels altered in response to p53 silencing.
Article
Surgery
Rossana Franzin, Alessandra Stasi, Fabio Sallustio, Stefania Bruno, Guido Merlotti, Marco Quaglia, Giuseppe Grandaliano, Paola Pontrelli, Joshua M. Thurman, Giovanni Camussi, Giovanni Stallone, Vincenzo Cantaluppi, Loreto Gesualdo, Giuseppe Castellano
Summary: Extracellular vesicles (EV) play an important role in the pathophysiology of antibody-mediated allograft rejection (AMR), inducing tubular senescence and endothelial to mesenchymal transition (EndMT) and regulating associated miRNA expression. These findings contribute to a better understanding of the mechanisms underlying AMR and provide potential targets for therapeutic intervention.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Review
Urology & Nephrology
Antonio Granata, Elnaz Rahbari, Francesco Pesce, Loreto Gesualdo, Matthias Zeiler
Summary: Contrast enhanced ultrasound (CEUS) is a novel examination method in the field of peritoneal dialysis, which has been found to be helpful in evaluating catheter malfunction, peritoneal-pleural communication, leakage, and herniation. It provides dynamic functional imaging of the catheter and its complications. CEUS is simple, safe, radiation-free, and appears to be less time-consuming and more cost-effective than other radiological imaging techniques.
JOURNAL OF NEPHROLOGY
(2022)
Review
Food Science & Technology
Gianvito Caggiano, Alessandra Stasi, Rossana Franzin, Marco Fiorentino, Maria Teresa Cimmarusti, Annamaria Deleonardis, Rita Palieri, Paola Pontrelli, Loreto Gesualdo
Summary: The gut microbiome plays a crucial role in kidney disease, with dysbiosis-related uremic toxins and pro-inflammatory mediators contributing to kidney function deterioration. The toxicity of uremic compounds has been well-documented in various pathophysiological mechanisms, such as cardiovascular injury, metabolic dysfunction, and inflammation. Accumulating evidence supports the use of fecal microbiota transplantation (FMT) as a promising treatment to reduce uremic toxin accumulation. This review presents the current state of FMT application in kidney disease to restore a healthy microbiome and reverse uremic toxin retention.
Article
Biochemistry & Molecular Biology
Gianvito Caggiano, Loredana Amodio, Alessandra Stasi, Nicola Antonio Colabufo, Santina Colangiulo, Francesco Pesce, Loreto Gesualdo
Summary: In recent years, indoxyl sulfate has been found to play a significant role in the progression of kidney disease and the development of cardiovascular problems. However, current methods for measuring indoxyl sulfate levels, such as LC-MS/MS, are time-consuming and require specialized equipment. In this pilot study, we developed a fast and simple alternative method using colorimetric assay to accurately measure indoxyl sulfate levels in serum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Fabio Sallustio, Angela Picerno, Francesca Montenegro, Maria Teresa Cimmarusti, Vincenzo Di Leo, Loreto Gesualdo
Summary: The human virome, consisting of prokaryotes, viruses, fungi, and parasites, is intricately connected with the human host. Different viral community states have been identified as indicators of health or undesirable outcomes for the host. The virome can collaborate with the human host in maintaining mutualistic functions and preserving human health. This Review explores the role of viruses in health and disease, the relationship between the virobiota and immune system control, and the potential molecular mechanisms linking viruses to glomerulonephritis and IgA nephropathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Davide Fiore Bavaro, Lucia Diella, Alessandra Belati, Giuliana Metrangolo, Laura De Santis, Vito Spada, Michele Camporeale, Angelo Dargenio, Gaetano Brindicci, Flavia Balena, Deborah Fiordelisi, Fabio Signorile, Giacomo Loseto, Crescenza Pasciolla, Carla Minoia, Immacolata Attolico, Tommasina Perrone, Simona Simone, Maria Rendina, Nicoletta Giovine, Francesco Di Gennaro, Pellegrino Musto, Attilio Guarini, Alfredo Di Leo, Loreto Gesualdo, Maria Dell'Aera, Annalisa Saracino
Summary: Remdesivir (REM) and monoclonal antibodies (mAbs) may alleviate severe COVID-19 in at-risk hospitalized patients, particularly in elderly or immunocompromised hosts. The use of REM and mAbs was associated with a reduced risk of severe COVID-19, and the combination therapy was particularly beneficial for immunocompromised patients.
Review
Cell Biology
Francesca Giannuzzi, Silvia Maiullari, Loreto Gesualdo, Fabio Sallustio
Summary: Long non-coding RNAs (lncRNAs) are a diverse group of transcripts that regulate gene expression at different levels in various cellular contexts and biological processes. Understanding their mechanisms of action and their involvement in disease can lead to potential therapeutic approaches. In the context of renal pathogenesis, the role of lncRNAs is still not well understood, particularly in healthy kidneys and adult renal stem/progenitor cells (ARPC). This review provides an overview of lncRNA biogenesis, degradation, and functions, focusing on their functional roles in kidney diseases and their regulation of stem cell biology, with a specific emphasis on the lncRNA HOTAIR's ability to prevent senescence in ARPC.
Article
Cell Biology
Rossana Franzin, Alessandra Stasi, Giuseppe De Palma, Angela Picerno, Claudia Curci, Serena Sebastiano, Monica Campioni, Antonella Cicirelli, Alessandro Rizzo, Vito Francesco Di Lorenzo, Paola Pontrelli, Giovanni Battista Pertosa, Giuseppe Castellano, Loreto Gesualdo, Fabio Sallustio
Summary: In this study, it was found that tubular adult renal progenitor cells (tARPC) can influence the gene expression of renal proximal tubular cell (RPTEC) during cisplatin-induced nephrotoxicity via the release of extracellular vesicles (EV) enriched with CYP1B1 and miR-27b-3p. CYP1B1 expression was correlated with renal function.
Article
Infectious Diseases
Davide Fiore Bavaro, Nicolo De Gennaro, Alessandra Belati, Lucia Diella, Roberta Papagni, Luisa Frallonardo, Michele Camporeale, Giacomo Guido, Carmen Pellegrino, Maricla Marrone, Alessandro Dell'Erba, Loreto Gesualdo, Nicola Brienza, Salvatore Grasso, Giuseppe Columbo, Antonio Moschetta, Giovanna Elisiana Carpagnano, Antonio Daleno, Anna Maria Minicucci, Giovanni Migliore, Annalisa Saracino
Summary: The introduction of a pro-active Infectious Disease (ID) consultation in a COVID-19 hospital can reduce the mortality risk of patients with MDROs infections by optimizing treatment and diagnostic strategies to improve patient outcomes.
Review
Biochemistry & Molecular Biology
Marco Fiorentino, Francesca Bagagli, Annamaria Deleonardis, Alessandra Stasi, Rossana Franzin, Francesca Conserva, Barbara Infante, Giovanni Stallone, Paola Pontrelli, Loreto Gesualdo
Summary: Kidney transplantation is the preferred treatment for end-stage renal disease (ESRD). Kidney transplant recipients (KTRs) are at a higher risk of ICU admission, mainly in the late post-transplant period. Urosepsis and bloodstream infections are the main reasons for ICU admissions, and side effects of immunosuppressive treatment should also be accounted for. AKI incidence is common in KTRs during ICU stay, and in-hospital mortality can be high. The present review aims to explore the impact of AKI on outcomes in KTRs and provide insights into its diagnosis, management, and immunosuppression modulation.
Meeting Abstract
Transplantation
Fabio Sallustio, Claudia Curci, Maria Teresa Cimmarusti, Angela Picerno, Francesca Giannuzzi, Giuseppe De Palma, Carmen Sivo, Francesca Annese, Giulia Fonto, Silvio Tafuri, Iris Cara, Vincenzo Barone, Alessandra Stasi, Francesco Pesce, Vincenzo Di Leo, Loreto Gesualdo
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Article
Urology & Nephrology
Francesco Pesce, Domenico Pasculli, Giuseppe Pasculli, Luca De Nicola, Mario Cozzolino, Antonio Granata, Loreto Gesualdo
Summary: Training and network support from nephrologists to GPs can improve CKD awareness and increase its identification in the general population and especially in categories at risk.
JOURNAL OF NEPHROLOGY
(2022)