Article
Cell Biology
Zhe Han, Xin Hao, Cheng-Jie Zhou, Jun Wang, Xin Wen, Xing-Yue Wang, De-Jian Zhang, Cheng-Guang Liang
Summary: The study investigated the expression profiles and functional roles of CLTC in sheep oocytes and found that CLTC plays crucial regulatory roles in sheep oocyte maturation and early embryo development, especially in spindle dynamics and early embryo development. The results indicate that CLTC is essential for spindle formation and chromosomal alignment, as well as for proper early embryo development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Danni Wang, Hongzheng Sun, Jiaqi Zhang, Zhenyue Huang, Congyang Li, Longsen Han, Yongan Xin, Shoubin Tang, Juan Ge, Qiang Wang
Summary: FKBP25 plays a crucial role in oocyte maturation by affecting spindle assembly, chromosome alignment, and kinetochore-microtubule attachment. Depletion of FKBP25 results in elevated aneuploidy incidence in oocytes. Reduced levels of FKBP25 protein in old oocytes can be partly rescued by ectopic expression of FKBP25, which is also found to have phosphorylation at serine 163 affecting its action on meiotic maturation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Guangyi Cao
Summary: The correct assembly and regulation of the spindle apparatus are crucial for the precise separation of chromosomes in mouse oocytes. NUSAP1 is a spindle-associated protein that plays an important role in oocyte meiosis maturation. Its depletion leads to chromosome misalignment, aneuploidy, and abnormal spindle assembly.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Cell Biology
Yue Xue, Tie-Gang Meng, Ying-Chun Ouyang, Sai-Li Liu, Jia-Ni Guo, Zhen-Bo Wang, Heide Schatten, Chun-Ying Song, Xing-Ping Guo, Qing-Yuan Sun
Summary: This study found that Miro1 plays a crucial role in the maturation process of mouse oocytes by regulating mitochondrial homeostasis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Yumi Hoshino, Takafumi Uchida
Summary: Pin1, an enzyme that regulates the function of phosphorylated proteins, plays a crucial role in meiotic maturation. By altering the structure of proline peptide bond in proteins, Pin1 controls the progression of meiosis and affects the extrusion of polar bodies. The functional regulation of Pin1 is essential for oocyte production and holds potential applications in reproductive medicine and animal breeding.
Article
Cell Biology
Wen Gao, Chen Zhang, Bichun Li, Jeong Su Oh
Summary: Azoxystrobin (AZO), a widely used fungicide in agriculture, has been found to impair mouse oocyte maturation by disturbing spindle formation, chromosome alignment, mitochondrial distribution, and increasing oxidative stress. The toxicity of AZO to oocytes can be relieved by melatonin supplementation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Dongmei Deng, Juan Xie, Yin Tian, Ling Zhu, Xuemei Liu, Junxia Liu, Guoning Huang, Jingyu Li
Summary: Acquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation. Two approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage or post-IVM at the metaphase II (MII) stage. This study found that oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Bing-Wang Zhao, Si-Min Sun, Ke Xu, Yuan-Yuan Li, Wen-Long Lei, Li Li, Sai-Li Liu, Ying-Chun Ouyang, Qing-Yuan Sun, Zhen-Bo Wang
Summary: This study uncovered the important role of F-box protein FBXO34 in oocyte meiotic maturation, where its depletion resulted in failure of meiotic resumption and overexpression promoted germinal vesicle breakdown. Additionally, overexpression of FBXO34 also led to continuous activation of the spindle assembly checkpoint and subsequent MI arrest in oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wei Yue, Yue Wang, Tie-Gang Meng, Hong-Yong Zhang, Xin-Ran Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Summary: This study investigates the role of outer kinetochore protein KNL1 in mouse oocytes and reveals its critical function in ensuring timely and accurate chromosome segregation during oocyte meiotic maturation.
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jun Han, Shuo Wang, Huarong Wang, Tuo Zhang, Ye Yang, Ting Zhao, Ziqi Chen, Guoliang Xia, Chao Wang
Summary: Impaired fertility is a major side effect of chemotherapy for female cancer patients due to damage to oocyte quality. This study demonstrates that reduced expression of the antioxidant enzyme SIRT1 exacerbates the effects of the chemotherapy drug doxorubicin on oocyte maturation. Increasing SIRT1 expression can partially alleviate these effects and improve fertilization ability and developmental competence of oocytes and embryos.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2023)
Article
Obstetrics & Gynecology
Usama Al-Zubaidi, Deepak Adhikari, Ozgur Cinar, Qing-Hua Zhang, Wai Shan Yuen, Michael P. Murphy, Luk Rombauts, Rebecca L. Robker, John Carroll
Summary: The study showed that MitoQ and BGP-15 during in vitro maturation can protect against spindle and chromosomal defects in oocytes exposed to oxidative stress and from aged mice. In human oocytes, MitoQ promotes nuclear maturation and protects against chromosomal misalignments. These findings suggest that mitochondrially targeted molecules could be a novel therapeutic approach against maternal aging-related spindle and chromosomal abnormalities.
HUMAN REPRODUCTION
(2021)
Article
Cell Biology
Song-Hee Lee, Ming-Hong Sun, Dongjie Zhou, Wen-Jie Jiang, Xiao-Han Li, Geun Heo, Xiang-Shun Cui
Summary: Exposure to high temperature has been found to negatively affect the development of porcine oocytes. It induces mitochondrial dysfunction, ER stress, abnormal Golgi apparatus distribution, and lysosomal damage, leading to the failure of meiotic maturation in the oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Reproductive Biology
Yue Wang, Chun-Hua Xing, Shun Chen, Shao-Chen Sun
Summary: The study showed that ZEN exposure disrupted organelle function in porcine oocyte meiotic maturation, affecting mitochondria, endoplasmic reticulum, Golgi apparatus, and lysosome.
Article
Environmental Sciences
Sheng-Ji Yang, Yong-Sheng Wang, Li-Dan Zhang, Zhi-Ming Ding, Xu Zhou, Ze-Qun Duan, Ming Liu, Ai-Xin Liang, Li-Jun Huo
Summary: This study demonstrated that exposure to propyl gallate (PG) compromised oocyte meiotic maturation in mice by inducing mitochondrial stress, activating apoptosis, inducing DNA damage, and increasing oxidative stress, as well as altering histone methylation level.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Reproductive Biology
Dong-Hui Wang, Hong-Xia Zhou, Shu-Jun Liu, Cheng-Jie Zhou, Xiang-Wei Kong, Zhe Han, Cheng-Guang Liang
Article
Cell Biology
Cheng-Jie Zhou, Xing-Yue Wang, Zhe Han, Dong-Hui Wang, Yu-Zhen Ma, Cheng-Guang Liang
Article
Pharmacology & Pharmacy
Lei Ge, Zhe Han, Yu-Qing Gao, Cheng-Jie Zhou, Dong-Hui Wang, Yu-Zhen Ma, Cheng-Guang Liang
Article
Biochemistry & Molecular Biology
Xue-Chen Wu, Zhe Han, Xin Hao, Yi-Tong Zhao, Cheng-Jie Zhou, Xin Wen, Cheng-Guang Liang
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Article
Multidisciplinary Sciences
Shu-Jun Liu, Jia-Bo Sun, Xin Hao, Zhe Han, Xin Wen, Xing-Yue Wang, Cheng-Jie Zhou, Cheng-Guang Liang
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Cheng-Jie Zhou, Dong-Hui Wang, Xiang-Wei Kong, Zhe Han, Xin Hao, Xing-Yue Wang, Xin Wen, Cheng-Guang Liang
Article
Environmental Sciences
Yu-Qing Gao, Lei Ge, Zhe Han, Xin Hao, Mei-Ling Zhang, Xiao-Jie Zhang, Cheng-Jie Zhou, De-Jian Zhang, Cheng-Guang Liang
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2020)
Article
Cell Biology
Zhe Han, Xin Hao, Cheng-Jie Zhou, Jun Wang, Xin Wen, Xing-Yue Wang, De-Jian Zhang, Cheng-Guang Liang
Summary: The study investigated the expression profiles and functional roles of CLTC in sheep oocytes and found that CLTC plays crucial regulatory roles in sheep oocyte maturation and early embryo development, especially in spindle dynamics and early embryo development. The results indicate that CLTC is essential for spindle formation and chromosomal alignment, as well as for proper early embryo development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Xin Hao, Yi-Tong Zhao, Kang Ding, Fang-Rui Xue, Xin-Yu Wang, Qi Yang, Zhe Han, Cheng-Guang Liang
Summary: This study investigated the impact of different assisted hatching methods on implantation and development of in vitro cultured embryos, and found that drilling a small hole (SH) on zona pellucida during morula stage can increase hatch rate, facilitate implantation, and increase litter size without affecting birth weight, growth, or gender ratio in offspring. This approach could be an effective and reliable assisted hatching method worth further investigation in human-assisted reproductive technology.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Environmental Sciences
Lei Ge, Yu-Qing Gao, Zhe Han, Shu-Jun Liu, Xing-Yue Wang, Xiao-Jie Zhang, Rui-Hao Tang, Rui-Feng Zhang, Dui Sun, Bo Feng, De-Jian Zhang, Cheng-Guang Liang
Summary: This study demonstrates that Olaquindox (OLA) intake has detrimental effects on sperm quality and offspring survival in male mice. High-dose OLA administration leads to changes in body weight, organ weights, and the morphology of the testis seminiferous tubules. It also reduces sperm count, motility, and viability. Both low and high doses of OLA induce oxidative damage, early apoptosis, and abnormal mitochondrial membrane potential in sperm. While high-dose OLA impairs the development of in vitro fertilized embryos, it does not affect the natural fertility of males, although paternal OLA intake significantly decreases offspring survival rate from the age of 4 weeks.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Reproductive Biology
Chun-Ru Song, Ru-Jing Zhang, Fang-Rui Xue, Xiao-Jie Zhang, Xing-Yue Wang, Dui Sun, Kang Ding, Qi Yang, Xin-Yu Wang, Cheng-Guang Liang
Summary: In this study, sheep were used as an animal model to investigate the regulatory mechanisms of cis-elements in the cytoplasmic polyadenylation and translation of maternal mRNAs in domestic animal oocytes. The positioning and number of polyadenylation signal (PAS) and cytoplasmic polyadenylation element (CPE) in the 3'-UTR were found to affect the efficiency of translational control.
Article
Multidisciplinary Sciences
Cheng-Jie Zhou, Xing-Yue Wang, Yan-Hua Dong, Dong-Hui Wang, Zhe Han, Xiao-Jie Zhang, Qing-Yuan Sun, John Carroll, Cheng-Guang Liang
Summary: DRP1 is recruited to the kinetochore by CENP-F after nuclear envelope breakdown in mouse oocytes. Loss of DRP1 during different stages of the cell cycle results in premature chromosome segregation and abnormal degradation of cyclinB1 and securin. Additionally, DRP1 binding can restrain the E3 ligase activity of APC/C.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Xin Wen, Qi Yang, Dui Sun, Zhao-Yu Jiang, Teng Wang, Hao-Ran Liu, Zhe Han, Lu Wang, Cheng-Guang Liang
Summary: By using transcriptome sequencing and experimental verification of mouse cumulus cells and oocytes, this study revealed the unique characteristics of cumulus cells and oocytes through ligand-receptor interactions. The results showed that cumulus cells activated NF-kappa B signaling in oocytes through the IL1-IL1R1 interaction, leading to mitochondrial dysfunction, excessive ROS accumulation, and increased early apoptosis. This study provides a foundation for further understanding the molecular mechanism of POA and offers clues for exploring the relationship between cumulus cells and oocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Xin Wen, Zhe Han, Shu-Jun Liu, Xin Hao, Xiao-Jie Zhang, Xing-Yue Wang, Cheng-Jie Zhou, Yu-Zhen Ma, Cheng-Guang Liang
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)