Article
Environmental Sciences
Biao Jing, Wang Wan, Bo Hu, Wenhan Jin, Zhenduo Zhang, Congcong Peng, Mengdie Wang, Jintai Deng, Xuepeng Dong, Yu Liu, Zhenming Gao
Summary: Plastic nanoparticles can deteriorate proteome stability, compromise cellular protein homeostasis, and cause global proteome misfolding and aggregation, leading to negative impacts on cellular fitness.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Plant Sciences
Hyo-Gil Choi, Dong-Young Park, Nam-Jun Kang
Summary: This study investigates the changes in proteome expression of different tomato genotypes at different ripening stages. The results show that the number of protein spots differs among tomato genotypes and ripening stages. Protein spots related to metabolism, energy, protein synthesis, and interaction with the cellular environment show significant changes according to fruit color at different ripening stages.
Article
Biology
Peter H. Lee, Michael Anaya, Mark S. Ladinsky, Justin M. Reitsma, Kai Zinn, K. VijayRaghavan
Summary: Communication between distant cells can be achieved through the delivery of proteins and RNAs by extracellular vesicles (EVs). This study reveals that the cell-surface protein Sas serves as a targeting ligand for EVs. Sas binds to the receptor tyrosine phosphatase Ptp10D, and Sas-bearing EVs preferentially target cells expressing Ptp10D.
Review
Cell Biology
Jiyao Song, Johannes M. Herrmann, Thomas Becker
Summary: Mitochondria contain a diverse array of proteins from two genomes and require synchronized gene expression for proper function. The mitochondrial proteome is highly adaptable and regulated by quality control systems to ensure cellular adaptation and functionality. Defects in mitochondrial protein quality control mechanisms can lead to proteotoxic insults and cell death, highlighting the critical importance of maintaining protein homeostasis in mitochondria.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Korbinian Kienle, Katharina M. Glaser, Sarah Eickhoff, Michael Mihlan, Konrad Knoepper, Eduardo Reategui, Maximilian W. Epple, Matthias Gunzer, Ralf Baumeister, Teresa K. Tarrant, Ronald N. Germain, Daniel Irimia, Wolfgang Kastenmueller, Tim Laemmermann
Summary: The study found that neutrophils have evolved an intrinsic mechanism to limit self-aggregation during inflammation, with GPCR desensitization acting as a negative feedback control to stop migration when high concentrations of attractants are detected. However, interfering with this process may hinder bacterial clearance, as bacteria proliferation within neutrophil clusters becomes impeded. This highlights the delicate balance of neutrophil chemotaxis and arrest in combating bacterial escape.
Article
Biochemistry & Molecular Biology
Kuan-Yu Lai, Sebastien R. G. Galan, Yibo Zeng, Tianhui Hina Zhou, Chang He, Ritu Raj, Jitka Riedl, Shi Liu, K. Phin Chooi, Neha Garg, Min Zeng, Lyn H. Jones, Graham J. Hutchings, Shabaz Mohammed, Satish K. Nair, Jie Chen, Benjamin G. Davis, Wilfred A. van der Donk
Summary: LanCL catalyzes the addition of glutathione to Dha/Dhb in proteins, driving irreversible C-glutathionylation to repair damages in proteins, indicating its physiological importance in removing reactive electrophiles and dysregulatory effects from the proteome.
Article
Multidisciplinary Sciences
Joel G. Rurik, Istvan Tombacz, Amir Yadegari, Pedro O. Mendez Fernandez, Swapnil Shewale, Li Li, Toru Kimura, Ousamah Younoss Soliman, Tyler E. Papp, Ying K. Tam, Barbara L. Mui, Steven M. Albelda, Ellen Pure, Carl H. June, Haig Aghajanian, Drew Weissman, Hamideh Parhiz, Jonathan A. Epstein
Summary: This study developed a therapeutic approach to generate antifibrotic CAR T cells in vivo by delivering modified mRNA in T cell-targeted nanoparticles. The efficacy of these in vivo-reprogrammed CAR T cells was evaluated in a mouse model of heart failure.
Article
Environmental Sciences
Yuchun Zhang, Rong Liang, Yingxiao Chen, Yaling Wang, Xue Li, Shang Wang, Honglin Jin, Lusha Liu, Zijian Tang
Summary: This study suggests that HSF1 plays a protective role in cells exposed to cadmium toxicity by maintaining proteome and genome integrity. Overexpression of HSF1 reduces protein aggregates and DNA damages caused by cadmium.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biology
Megan K. Elder, Hediye Erdjument-Bromage, Mauricio M. Oliveira, Maggie Mamcarz, Thomas A. Neubert, Eric Klann
Summary: Alzheimer's disease is associated with protein synthesis dysregulation in the hippocampus, leading to metabolic alterations that persist throughout the progression of the disease. Early changes in protein synthesis precede symptom onset and may contribute to cellular dysfunction in Alzheimer's disease.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Matthew Domnauer, Fan Zheng, Liying Li, Yanxiao Zhang, Catherine E. Chang, Jay R. Unruh, Juliana Conkright-Fincham, Scott McCroskey, Laurence Florens, Ying Zhang, Christopher Seidel, Benjamin Fong, Birgit Schilling, Rishi Sharma, Arvind Ramanathan, Kausik Si, Chuankai Zhou
Summary: The study shows that budding yeast responds to long-term high temperature challenge by switching from chaperone induction to reduction of temperature-sensitive proteins and re-localizing a portion of its proteome. Additionally, many proteins adopt an alternative conformation. These findings reveal mechanisms of adaptation to prolonged temperature change in organisms.
Article
Biochemical Research Methods
Yan Wang, Haitao Ge, Zhen Xiao, Chengcheng Huang, Gaojie Wang, Xiaoxiao Duan, Limin Zheng, Jinghui Dong, Xiahe Huang, Yuanya Zhang, Hongyu An, Wu Xu, Yingchun Wang
Summary: Spatial proteome reorganization is an important adaptation mechanism for photosynthetic organisms in addition to the differential expression of stress responsive genes. In this study, we treated a unicellular photosynthetic model cyanobacterium with different types of abiotic stresses and identified proteins showing stress induced differential expression and/or redistribution between cellular compartments. Our findings provide insights into the regulatory mechanism underlying stress responses and serve as a valuable resource for future studies on photosynthetic organisms' adaptation to adverse environments.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Yan Wang, Haitao Ge, Zhen Xiao, Chengcheng Huang, Gaojie Wang, Xiaoxiao Duan, Limin Zheng, Jinghui Dong, Xiahe Huang, Yuanya Zhang, Hongyu An, Wu Xu, Yingchun Wang
Summary: This study identified proteins in a photosynthetic cyanobacterium that undergo differential expression and/or redistribution in response to different abiotic stresses. These findings provide insights into the regulatory mechanisms underlying stress responses and serve as an important resource for future studies on how photosynthetic organisms cope with adverse environments.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Immunology
Anna Fernanda Vasconcellos, Reynaldo Magalhaes Melo, Samuel Coelho Mandacaru, Lucas Silva de Oliveira, Athos Silva de Oliveira, Emily Caroline dos Santos Moraes, Monique Ramos de Oliveira Trugilho, Carlos Andre Ornelas Ricart, Sonia Nair Bao, Renato Oliveira Resende, Sebastien Charneau
Summary: Chikungunya virus (CHIKV) is a single-stranded positive RNA virus transmitted to humans by infected mosquitoes. Infection with CHIKV causes painful symptoms in humans. In this study, CHIKV infection in Ae. aegypti Aag-2 cells was observed using proteomic analysis and transmission electron microscopy. The results showed high viral cargo and elongated mitochondria in the infected cells. Proteome analysis identified regulated protein groups related to protein synthesis, energy metabolism, signaling pathways, and apoptosis. These regulated proteins might play roles in antiviral and/or proviral mechanisms and the balance between viral propagation and host cell survival, possibly contributing to persistent infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Aditi Sahu, Kivanc Kose, Lukas Kraehenbuehl, Candice Byers, Aliya Holland, Teguru Tembo, Anthony Santella, Anabel Alfonso, Madison Li, Miguel Cordova, Melissa Gill, Christi Fox, Salvador Gonzalez, Piyush Kumar, Amber Weiching Wang, Nicholas Kurtansky, Pratik Chandrani, Shen Yin, Paras Mehta, Cristian Navarrete-Dechent, Gary Peterson, Kimeil King, Stephen Dusza, Ning Yang, Shuaitong Liu, William Phillips, Pascale Guitera, Anthony Rossi, Allan Halpern, Liang Deng, Melissa Pulitzer, Ashfaq Marghoob, Chih-Shan Jason Chen, Taha Merghoub, Milind Rajadhyaksha
Summary: The response to immunotherapies is unpredictable and variable. Traditional pathology-based phenotyping of tumors is unable to accurately predict treatment response to immunotherapy. Researchers have developed a non-invasive in vivo imaging technique using reflectance confocal microscopy to combine inflammation profiles with vascular features, allowing for dynamic cellular-level phenotyping of the tumor immune microenvironment. This approach provides a more reliable predictor of response to immune therapy in skin cancer patients.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Tomasz Deptuch, Karolina Penderecka, Mariusz Kaczmarek, Sara Molenda, Hanna Dams-Kozlowska
Summary: Bioengineered MS1 silk functionalized with the H2.1 peptide has been investigated as a drug delivery system targeted at Her2-overexpressing cancer cells. The immunogenicity of MS1-type silk drug carriers was analyzed, and the results showed that H2.1MS1 spheres exhibited higher immune activity and multiple injections did not hinder drug delivery or induce toxicity.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Horlando Carlota da Silva, Aurelio de Oliveira Monteiro, Francisco Wagner de Queiroz Almeida-Neto, Emanuelle Machado Marinho, Maria Kueirislene Amancio Ferreira, Francisco Rogenio da Silva Mendes, Emmanuel Silva Marinho, Marcia Machado Marinho, Lucas Lima Bezerra, Matheus Nunes da Rocha, Jane Eire Silva Alencar de Menezes, Alexandre Magno Rodrigues Teixeira, Antonio Wlisses da Silva, Emanuela de Lima Reboucas, Francisco das Chagas Lima Pinto, Helcio Silva dos Santos, Gilvandete Maria Pinheiro Santiago
Summary: Diabetes mellitus is a chronic metabolic disorder that has been increasing drastically worldwide. The study found that fisetinidol, a compound obtained from plants, has potential hypoglycemic and hepatoprotective effects, and shares the same inhibitory mechanisms as existing antidiabetic drugs.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Obstetrics & Gynecology
Victor Blasco, Francisco M. Pinto, Ainhoa Fernandez-Atucha, Nicolas Prados Dodd, Manuel Fernandez-Sanchez, Luz Candenas
Summary: Neurokinin B and its receptors have a significant role in the regulation of female reproduction. Abnormal expression of these molecules may be associated with decreased fertility in women with infertility, providing new insights for diagnosis and treatment.
REPRODUCTIVE SCIENCES
(2023)
Article
Plant Sciences
Joana Figueiredo, Rita B. B. Santos, Leonor Guerra-Guimaraes, Celine C. Leclercq, Jenny Renaut, Lisete Sousa, Andreia Figueiredo
Summary: This study evaluates the modulation of grapevine proteome in the apoplast during the first 6 hours after Plasmopara viticola inoculation, revealing the dynamics of cellular communication between grapevine and the pathogen. The results showed that the tolerant grapevine cultivar triggers defense mechanisms such as pathogen recognition, cell wall modifications, reactive oxygen species signaling, and secretion of proteins to disrupt pathogen structures. Meanwhile, the pathogen proteins related to development and virulence were found to be predominant. This pioneer study highlights the significance of early cellular communication in grapevine defense.
Review
Clinical Neurology
Susana Lopez-Ortiz, Simone Lista, Pedro L. Valenzuela, Jose Pinto-Fraga, Ricardo Carmona, Filippo Caraci, Giuseppe Caruso, Nicola Toschi, Enzo Emanuele, Audrey Gabelle, Robert Nistico, Francesco Garaci, Alejandro Lucia, Alejandro Santos-Lozano
Summary: The study summarizes the relationship between physical activity and the development of Alzheimer's disease, as well as the impact of exercise on patients with Alzheimer's disease. The findings suggest that physical activity can lower the risk of Alzheimer's disease and exercise has positive effects on cognitive function, physical performance, and functional independence.
JOURNAL OF NEUROLOGY
(2023)
Article
Environmental Sciences
Roberto Berni, Celine C. Leclercq, Philippe Roux, Jean-Francois Hausman, Jenny Renaut, Gea Guerriero
Summary: In the last decade, the exploration of deep space has been a significant objective for many national space programs. The aim is to expand human presence in the solar system and accelerate innovation through exploration and knowledge. This study focuses on the molecular analysis of a representative plant, Lolium multiflorum Lam., grown on potting soil and a Mars regolith simulant. The results show changes in gene expression and proteomics, as well as physical and chemical modifications in the regolith simulant caused by the plant's growth. These findings provide valuable insights into the major factors influencing plant growth on Mars simulant.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Oncology
Celia Garcia-Chico, Susana Lopez-Ortiz, Saul Penin-Grandes, Jose Pinto-Fraga, Pedro L. Valenzuela, Enzo Emanuele, Claudia Ceci, Grazia Graziani, Carmen Fiuza-Luces, Simone Lista, Alejandro Lucia, Alejandro Santos-Lozano
Summary: The prevalence of breast cancer is increasing and there is a need to investigate the molecular pathways that influence its progression. This review aims to describe the effects of physical exercise on breast cancer hallmarks, which are associated with the development of the disease. Regular physical exercise has positive effects on all major hallmarks of breast cancer and may help counteract its progression.
Article
Chemistry, Physical
Francisco J. Pinto, Jose Toledo, Matias Birrell, Ramiro Bazaez, Francisco Hernandez, Rodrigo Astroza
Summary: Bridges are crucial structures in a country's logistic chain and designing them to be resilient is important. Performance-based seismic design (PBSD) using nonlinear Finite Element (FE) models can predict the response and potential damage of different structural components under earthquake excitations. This study proposes a Bayesian probabilistic framework to update the parameters of seismic bars and elastomeric bearings based on actual data from comprehensive experimental campaigns.
Article
Agronomy
Petko Mladenov, Sibel Aziz, Elena Topalova, Jenny Renaut, Sebastien Planchon, Aamir Raina, Nasya Tomlekova
Summary: Drought stress has a negative impact on edible vegetable production globally, including economically important crops such as common bean (Phaseolus vulgaris L.) that heavily rely on rainfall or irrigation. This study summarized phenotypic data of 26 Bulgarian common bean mutant lines and cultivars exposed to drought stress, and evaluated the drought stress response using chlorophyll fluorescence and proteomics approaches. Different responses were observed among the mutant lines as several basic photosynthetic parameters were examined. Additionally, a relationship was found between productivity and photosynthetic performance, as well as the expression of key proteins related to stress response. These findings provide support for the selection of drought tolerant common bean lines for sustainable agriculture.
Article
Pediatrics
Carmen Matey-Rodriguez, Susana Lopez-Ortiz, Saul Penin-Grandes, Jose Pinto-Fraga, Pedro L. Valenzuela, Monica Pico, Carmen Fiuza-Luces, Simone Lista, Alejandro Lucia, Alejandro Santos-Lozano
Summary: This study aimed to analyze the accuracy of Evenson cut-points for estimating moderate-to-vigorous intensity physical activity (MVPA) and sedentary behavior (SB) in children and adolescents with disabilities, as well as to define new equations for estimating energy expenditure (EE) in this population, particularly in those with cerebral palsy (CP). Specific GT3X+ cut-points were also defined for estimating MVPA in individuals with CP. The results indicate that using specific ActiGraph GT3X+ cut-points is accurate for estimating MVPA levels in children and adolescents with disabilities, especially those with CP, at least in laboratory conditions.
Article
Nanoscience & Nanotechnology
Gea Guerriero, Flavia Maria Sutera, Jonas Hoffmann, Celine C. Leclercq, Sebastien Planchon, Roberto Berni, Jean-Francois Hausman, Jenny Renaut, Nissim Torabi-Pour, Holly Cherise Pennington, Mukhtar Ahmed, Michael Welsh, Ashkan Dehsorkhi, Suzanne Saffie-Siebert
Summary: This study investigated the use of nanoporous silicon-stabilized hybrid lipid nanoparticles as delivery systems for the flavonoid quercetin to protect tomato plants against salinity. The formulations, known as phyto-couriers, showed promising results in enhancing the crop's abiotic stress tolerance. They mitigated stress symptoms and upregulated proteins involved in cytoprotection and chromatin remodeling.
ACS APPLIED NANO MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Marina L. Garcia-Vaquero, Marjorie Heim, Barbara Flix, Marcelo Pereira, Lucile Palin, Tania M. Marques, Francisco R. Pinto, Javier de las Rivas, Aaron Voigt, Florence Besse, Margarida Gama-Carvalho
Summary: This study demonstrates that SMA and ALS disease-associated genes linked to RNA metabolism functionally converge on neuronal protein complexes, providing a new hypothesis to explain the common motor neuron phenotype. In addition, the study found that these functional modules are enriched in orthologues of human neuronal disease genes.
Article
Cell Biology
Filipe Magalhaes, Claudia Andrade, Beatriz Simoes, Fredi Brigham, Ruben Valente, Pedro Martinez, Jose Rino, Michela Sugni, Ana Varela Coelho
Summary: The regenerative potential varies among animals, with starfish showing outstanding abilities in this aspect. In contrast, humans have limited abilities to restore organ systems. Therefore, studying the regeneration process in starfish could provide insights for the development of new therapeutic approaches in humans. This study examined the anatomical and behavioral traits, coelomocyte populations, and neuronal tissue architecture during starfish central nervous system regeneration, revealing several anatomic anomalies and induced behavioral modifications. A new coelomocyte population was also discovered, suggesting its potential involvement in radial nerve cord regeneration.
CELL AND TISSUE RESEARCH
(2023)
Article
Plant Sciences
Malika Chabi, Estelle Goulas, Dmitry Galinousky, Anne-Sophie Blervacq, Anca Lucau-Danila, Godfrey Neutelings, Sebastien Grec, Arnaud Day, Brigitte Chabbert, Katharina Haag, Joerg Muessig, Sandrine Arribat, Sebastien Planchon, Jenny Renaut, Simon Hawkins
Summary: One of the challenges in utilizing plant fibers is understanding the molecular factors affecting fiber fineness and mechanical properties. By analyzing gene expression in flax fiber tissues from different varieties, researchers identified differentially expressed genes related to cell wall metabolism. Proteomic analysis revealed proteins associated with morphometric differences between varieties. The study also highlighted genes correlated with fiber mechanical properties. Overall, this research provides insight into the molecular factors influencing fiber quality in flax.
FRONTIERS IN PLANT SCIENCE
(2023)