Article
Multidisciplinary Sciences
Yujing Hu, Jiayi Ji, Hao Cheng, Rongli Luo, Jie Zhang, Wenjing Li, Xingsui Wang, Jie Zhang, Yuncong Yao
Summary: This study aims to elucidate how miR408a and its target BBP regulate Cu homeostasis, ROS homeostasis, and anthocyanin biosynthesis in Malus plants.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Plant Sciences
Abdul Rehman, Ismail Khan, Muhammad Farooq
Summary: Supra-optimal temperature has negative effects on plant growth and development, especially during the reproductive stage. The heat tolerance of plants depends on various factors including heat stress responses, signaling pathways, and plant hormone biosynthesis. The production of secondary metabolites plays a crucial role in enhancing a plant's ability to cope with high temperature stress.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Huan Chen, Qiujun Lin, Zhuo Li, Jin Chu, Hai Dong, Qiong Mei, Yuanhu Xuan
Summary: The molecular mechanism of rice defence against sheath blight (ShB) involves the involvement of CIPK31, which is found to increase resistance to ShB. CIPK31 interacts with RBOHA and CatC through different pathways, affecting the accumulation of H2O2.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Xiu-Qing Jing, Meng-Ru Zhou, Xiu-Min Nie, Lu Zhang, Peng-Tao Shi, Abdullah Shalmani, Hai Miao, Wen-Qiang Li, Wen-Ting Liu, Kun-Ming Chen
Summary: OsGSTU6, a novel tau class GST gene in rice, plays a crucial role in regulating Cd stress tolerance by maintaining intracellular ROS homeostasis as well as reducing Cd accumulation in leaves through overexpression, and increasing Cd accumulation and decreasing stress tolerance through knockdown.
JOURNAL OF PLANT GROWTH REGULATION
(2021)
Article
Multidisciplinary Sciences
Eryun Zhang, Lihua Jin, Yangmeng Wang, Jui Tu, Ruirong Zheng, Lili Ding, Zhipeng Fang, Mingjie Fan, Ismail Al-Abdullah, Rama Natarajan, Ke Ma, Zhengtao Wang, Arthur D. Riggs, Sarah C. Shuck, Li Yang, Wendong Huang
Summary: This study reveals that AMPK in the intestine modulates brown adipose tissue thermogenesis and affects energy balance and metabolic disorders. These findings provide important insights into the mechanism of metformin, a drug used to treat diabetes.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Khalidah Ibraheem, Albashir M. A. Yhmed, Mohamed M. Nasef, Nikolaos T. Georgopoulos
Summary: CD40 accelerates the death of colorectal carcinoma cells by simultaneously activating both intrinsic and extrinsic pathways, highlighting its multifaceted properties.
Article
Plant Sciences
Ping Luo, Linmei Chen, Yeni Chen, Yuxiao Shen, Yongyi Cui
Summary: The C2H2 zinc finger transcription factor RmZAT10 plays an important role in cold tolerance by regulating proline biosynthesis and ROS homeostasis. Overexpression of RmZAT10 enhances cold tolerance in tobacco, while silencing of RmZAT10 increases cold sensitivity in Rosa multiflora. Overall, RmZAT10 is a key regulator in cold stress response.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Surupa Chakraborty, Esha Sircar, Camelia Bhattacharyya, Ankita Choudhuri, Akansha Mishra, Sreejita Dutta, Sneha Bhatta, Kumar Sachin, Rajib Sengupta
Summary: S-nitrosylation of proteins, resulting from the reaction between cysteine thiols and nitric oxide (NO), is associated with diseases and protein malfunction. It has rapidly emerged as a major cause of various metabolic, respiratory, and cardiac disorders, particularly cancer and neurodegeneration. The field of redox biochemistry has explored other covalently modified versions of proteins, but the S-nitrosoproteome, which refers to the diverse and dynamic repertoire of S-nitrosylated proteins, remains relatively unexplored. Research in this area is uncovering the clinical importance of S-nitrosylation in disease etiology and providing new avenues for prompt diagnosis.
Article
Biochemistry & Molecular Biology
Jannatul Fardus, Md. Shahadat Hossain, Masayuki Fujita
Summary: Salt stress negatively affects the growth and physiological parameters of lentil seedlings, but exogenous l-glutamic acid pretreatment can alleviate the damage caused by salt stress by maintaining ion homeostasis and enhancing antioxidant enzyme activity.
Article
Plant Sciences
Galina N. Veremeichik, Tatiana Y. Gorpenchenko, Tatiana V. Rusapetova, Evgenia V. Brodovskaya, Galina K. Tchernoded, Dmitry V. Bulgakov, Yurii N. Shkryl, Victor P. Bulgakov
Summary: This study investigated the stable pRiA4-transformed callus culture of Rubia cordifolia L. and found that the transformed tissue can achieve long-term cultivation without the need for plant growth regulators (PGRs). Moreover, the transformed tissue exhibited higher productivity of anthraquinone (AQ) compared to untransformed tissue. These findings have important implications in selecting optimal PGR combinations for the cultivation of rare, medicinal, and woody plants, and highlight the potential application of genetic modification in plant biotechnology.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Maria Babak, Dohyun Ahn
Summary: Copper plays a crucial role in cellular growth and development and its imbalance can lead to toxicity. This review explores the mechanisms of intracellular copper balance interference in tumor development and progression, and discusses the potential of using copper-depleting and copper-overloading conditions for improved patient outcomes. Additionally, the advantages of pre-formed copper complexes in comparison to in situ formed complexes are highlighted.
Article
Plant Sciences
Jingbin Li, Shang Zhao, Xue Yu, Wei Du, He Li, Ying Sun, Hao Sun, Chengjiang Ruan
Summary: Yellowhorn is an important edible woody oil tree species endemic to China. In response to combined drought and heat stress, MYB transcription factor XsMYB44 acts as a positive regulator in yellowhorn by triggering stomatal closure to maintain water levels and modulating ROS homeostasis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Review
Plant Sciences
Jaspreet Kour, Kanika Khanna, Arun Dev Singh, Shalini Dhiman, Tamanna Bhardwaj, Kamini Devi, Neerja Sharma, Puja Ohri, Renu Bhardwaj
Summary: Calcium (Ca2+) is an essential macronutrient for plants, playing crucial roles in various functions, especially under stressful conditions. It exhibits significant effects on cell wall weakening and other symptoms at low concentrations or insufficiency. Calcium is taken up by roots through channels or proteins and functions as a signaling molecule in the cytosol, eventually being transported and transmitted by various proteins and channels. Ca2+ also acts as a secondary messenger in physiological, stress-related, and developmental events.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Immunology
Mariangeles Clauzure, Angel G. Valdivieso, Andrea Dugour, Consuelo Mori, Maria M. Massip-Copiz, Maria A. Aguilar, Veronica Sotomayor, Cristian J. A. Asensio, Juan M. Figueroa, Tomas A. Santa-Coloma
Summary: The modulation of intracellular chloride (Cl-) concentration indirectly affects the activity of NLRP3 and CASP1, potentially through SGK1 mediation. The autocrine positive feedback loop involves multiple signaling molecules and pathways that regulate the secretion of IL-1 beta.
Article
Biotechnology & Applied Microbiology
Yang Zhang, Ruhong Ming, Madiha Khan, Yue Wang, Bachar Dahro, Wei Xiao, Chunlong Li, Ji-Hong Liu
Summary: Plant ethylene-responsive factor PtrERF9 is up-regulated under cold stress, positively affecting freezing tolerance by modulating ROS homeostasis and activating PtrGSTU17 and PtrACS1 genes. PtrERF9 acts downstream of ethylene signaling, forming a feedback regulation loop to reinforce the transcriptional regulation of its target genes.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Gastroenterology & Hepatology
Svetlana F. Lima, Lasha Gogokhia, Monica Viladomiu, Lance Chou, Gregory Putzel, Wen-Bing Jin, Silvia Pires, Chun-Jun Guo, Ylaine Gerardin, Carl Crawford, Vinita Jacob, Ellen Scherl, Su-Ellen Brown, John Hambor, Randy S. Longman
Summary: This study identified transferable, donor-derived bacterial strains that correlate with clinical response to FMT in UC, with Odoribacter splanchnicus revealed as a key component promoting metabolic and immune cell protection from colitis. These mechanistic features will help enhance the efficacy of microbial therapy for UC.
Article
Immunology
Lavoisier Akoolo, Silvia Pires, Jisun Kim, Dane Parker
Summary: The study reveals that type I interferon (IFN) response can be induced by A. baumannii infection, which is dependent on TLR4-TRIF-IRF3 signaling pathway and bacterial phagocytosis. Enhanced IFN induction was observed in phase variants of A. baumannii with reduced capsule and in capsule-deficient strains, accompanied by increased phagocytosis and host cell-mediated killing. The absence of the capsule also led to increased levels of several cytokines, including proinflammatory IL-6. However, the role of this pathway was not observed in vivo. This study highlights the importance of phase variation in modulating host immune responses and the protective role of A. baumannii capsule against host cell killing and activation of innate immunity.
JOURNAL OF INNATE IMMUNITY
(2022)
Article
Oncology
Ana Teresa Pinto, Ana Beatriz Machado, Hugo Osorio, Marta Laranjeiro Pinto, Rui Vitorino, Goncalo Justino, Catia Santa, Flavia Castro, Tania Cruz, Carla Rodrigues, Jorge Lima, Jose Luis R. Sousa, Ana Patricia Cardoso, Rita Figueira, Armanda Monteiro, Margarida Marques, Bruno Manadas, Jarne Pauwels, Kris Gevaert, Marc Mareel, Sonia Rocha, Tiago Duarte, Maria Jose Oliveira
Summary: Resistance to radiotherapy is a major problem in cancer treatment. Investigating the response of macrophages to ionizing radiation could help overcome tumor radio resistance.
Article
Fisheries
Joao Estevao, Hugo Osorio, Benjamin Costas, Andreia Cruz, Sergio Fernandez-Boo
Summary: This study characterized the haemolymph proteomic profiles of the grooved carpet shell in response to P. olseni infection. It identified biomarkers associated with tolerance and susceptibility, and found that tolerant clams have an efficient pathogen elimination mechanism and better metabolic regulation.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sofia Esteves, Clara Lima, Ines Costa, Hugo Osorio, Carmen Fernandez-Becerra, Nuno Santarem, Anabela Cordeiro-da-Silva
Summary: Dogs are susceptible to many life-threatening conditions like Canine Leishmaniosis (CanL), making it important to identify proteins associated with plasma extracellular vesicles (EVs) for biomarker development. In this study, EVs were recovered from healthy and CanL dogs' plasma and analyzed for their proteomic composition. CanL-associated proteins were identified, providing insights for biomarker discovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Juliana Pocas, Catarina Marques, Catarina Gomes, Andreia Hanada Otake, Filipe Pinto, Mariana Ferreira, Tiago Silva, Isabel Faria-Ramos, Rita Matos, Ana Raquel Ribeiro, Emanuel Senra, Bruno Cavadas, Silvia Batista, Joana Maia, Joana A. Macedo, Luis Lima, Luis Pedro Afonso, Jose Alexandre Ferreira, Lucio Lara Santos, Antonio Polonia, Hugo Osorio, Mattias Belting, Celso A. Reis, Bruno Costa-Silva, Ana Magalhaes
Summary: Gastric cancer is influenced by the expression of syndecan-4 (SDC4) which is associated with poor patient survival. SDC4 plays a crucial role in regulating the motility and invasion of gastric cancer cells. Additionally, SDC4 decorated with heparan sulfate is involved in the distribution, uptake, and functional effects of extracellular vesicles (EVs) derived from gastric cancer cells. SDC4 knockout disrupts the tropism of EVs for common gastric cancer metastatic sites. These findings provide insight into the molecular implications of SDC4 expression in gastric cancer cells and present potential therapeutic strategies targeting the glycan-EV axis to limit tumor progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Ritu Sharma, Travis E. Hartman, Tiago Beites, Jee-Hyun Kim, Hyungjin Eoh, Curtis A. Engelhart, Linnan Zhu, Daniel J. Wilson, Courtney C. Aldrich, Sabine Ehrt, Kyu Young Rhee, Dirk Schnappinger
Summary: The current treatment for tuberculosis (TB) infections is lengthy and requires multiple antibiotics. The development of shorter, simpler treatment regimens is critical to eradicating TB. NadE, an enzyme essential for the synthesis of the cofactor NAD, is considered a promising drug target. Through gene silencing and metabolomics, it has been shown that NADP deficiency is bacteriostatic, while NAD deficiency is bactericidal due to its role in Mtb's respiratory capacity. These findings suggest prioritizing NAD biosynthesis inhibitors in anti-TB drug development.
Article
Biology
Andrew Wong, Tiago Beites, Kyle A. Planck, Rachael A. Fieweger, Kathryn A. Eckartt, Shuqi Li, Nicholas C. Poulton, Brian C. VanderVen, Kyu Y. Rhee, Dirk Schnappinger, Sabine Ehrt, Jeremy Rock
Summary: The study found that the rv3645 gene plays an important role in Mycobacterium tuberculosis, participating in cAMP signaling and fatty acid metabolism. The loss of rv3645 results in increased sensitivity of M. tuberculosis to multiple antibiotics, and its essential function in bacterial growth is only required in the presence of long-chain fatty acids. This research demonstrates the crucial physiological regulatory functions of rv3645 and cAMP in M. tuberculosis.
Correction
Cell & Tissue Engineering
Marta R. Casanova, Hugo Osorio, Rui L. Reis, Albino Martins, Nuno M. Neves
NPJ REGENERATIVE MEDICINE
(2023)
Article
Biology
Celina Sao Jose, Carla Pereira, Marta Ferreira, Ana Andre, Hugo Osorio, Irene Gullo, Fatima Carneiro, Carla Oliveira
Summary: The cell architecture and adhesion between similar cells are maintained by cadherins. E-cadherin, encoded by CDH1 gene, plays a vital role in normal epithelia, while P-cadherin, encoded by CDH3 gene, is mainly present in tumors. The switch from E-cadherin to P-cadherin is common in several epithelial tumors, including gastric cancers. In this study, we revealed a mechanism for the E-cadherin to P-cadherin switch and found it to be associated with increased cell migration and proliferation in gastric cancers.
Article
Engineering, Biomedical
Francisca Diniz, Sofia Lamas, Hugo Osorio, Paulo Aguiar, Daniela Freitas, Fatima Gartner, Bruno Sarmento, Celso A. Reis, Joana Gomes
Summary: This study demonstrates the potential of using nanoparticles targeting STn for delivering FRT as a therapeutic approach in gastric cancer. The nanoparticles effectively reduced tumor growth, cell proliferation, and tumor necrosis in vivo.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Joana Peixoto, Catarina Principe, Ana Pestana, Hugo Osorio, Marta Teixeira Pinto, Hugo Prazeres, Paula Soares, Raquel T. Lima
Summary: LRP1B remains one of the most altered genes in cancer, but its relevance in cancer biology is still unclear. This study used a dual sgRNA CRISPR/Cas9 gene editing approach to disrupt LRP1B in glioblastoma cells and found that the edited cells exhibited altered morphology, increased size, changes in ploidy, and reduced cell growth. Proteomic analysis of the edited cells' secretome identified differentially expressed proteins that had not been previously associated with LRP1B alterations. Overall, this study provides new insights into the impact of LRP1B deletions in glioblastoma biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Yuanyuan Xu, Sabine Ehrt, Dirk Schnappinger, Tiago Beites
Summary: This study elucidates the mechanism of synthetic lethality of NADH dehydrogenases in Mycobacterium tuberculosis. NADH dehydrogenases have the functions of maintaining the NADH/NAD+ ratio and providing electrons to the respiratory chain, and the oxidation of NADH is essential for the viability of Mtb.
Article
Medicine, Research & Experimental
Cristiana Gaiteiro, Janine Soares, Marta Relvas-Santos, Andreia Peixoto, Dylan Ferreira, Paula Paulo, Andreia Brandao, Elisabete Fernandes, Rita Azevedo, Carlos Palmeira, Rui Freitas, Andreia Miranda, Hugo Osorio, Jesus Prieto, Luis Lima, Andre M. N. Silva, Lucio Lara Santos, Jose Alexandre Ferreira
Summary: This study focuses on the role of CD44 in bladder cancer and aims to address the molecular limitations related to CD44 through comprehensive experimental methods, potentially providing new targets for precision medicine.
Article
Chemistry, Multidisciplinary
Ana L. Graca, Manuel Gomez-Florit, Hugo Osorio, Marcia T. Rodrigues, Rui M. A. Domingues, Rui L. Reis, Manuela E. Gomes
Summary: This study produced and characterized platelet-derived EVs and investigated their effects on different cell types. The EVs were found to regulate stem cell differentiation, promote angiogenesis, and modulate macrophage polarization, suggesting their potential as therapeutic tools in tissue engineering and regenerative medicine.