Article
Chemistry, Multidisciplinary
Di Xin, Xiaochen Gai, Yidi Ma, Zexing Li, Qilin Li, Xiaochun Yu
Summary: In response to genotoxic stress-induced DNA damage, TopBP1 plays a key role in ATR activation for signaling transduction and DNA damage repair. The study reveals that TopBP1 interacts with pre-ribosomal RNA (pre-rRNA) and colocalizes with it at DNA double-strand breaks (DSBs). The BRCT4-5 domain of TopBP1 recognizes pre-rRNA and undergoes liquid-liquid phase separation (LLPS) with it, which may be responsible for the formation of TopBP1 foci at DSBs. The inhibition of pre-rRNA synthesis impairs TopBP1-mediated cell cycle checkpoint activation and homologous recombination repair.
Article
Immunology
Shengyuan Zhao, Julia A. Goewey A. Ruiz, Manu Sebastian, Dawit Kidane
Summary: This study reveals that a dRP lyase deficient variant of POLB increases mitotic dysfunction associated genomic instability, leading to a cytosolic DNA mediated inflammatory response. Additionally, inhibiting poly(ADP-ribose) polymerase 1 exacerbates chromosomal instability and enhances the cytosolic DNA mediated inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Shuiliang Ruan, Liping Zhai, Shasha Wu, Caiqun Zhang, Qiaobing Guan
Summary: This study found that SCFAs promote the formation of DNTs through OX40, regulating the activation of NLRP3 inflammasome and increasing the level of inflammatory factors in the central nervous system, providing a new mechanism for neuroinflammation in AD.
Article
Immunology
Yanhao He, Guangde Yang, Lijing Sun, Hongqian Gao, Feng Yao, Zhen Jin, Zihan Zheng, Lifang Chen, Weirong Wang, Nanbo Zheng, Rong Lin
Summary: SIRT6 protects against vascular inflammation through its deacetylase activity and the NRF2-dependent signaling pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Immunology
Qingyu Meng, Luya Pu, Qing Lu, Baisen Wang, Shuai Li, Bin Liu, Fan Li
Summary: Morin hydrate (MO) has anti-inflammatory and antioxidant effects, showing potential in treating atherosclerosis and inflammatory responses. MO inhibits atherosclerosis and promotes vascular endothelial autophagy by inhibiting autophagy pathways mediated by the NF kappa B signaling pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Cell Biology
Madhumanti Barman, Debabrata Dandasena, Akash Suresh, Vasundhra Bhandari, Sonam Kamble, Sakshi Singh, Madhusmita Subudhi, Paresh Sharma
Summary: This study found that artemisinin and its derivatives have potent activity against Theileria-infected cells and induce cell death through apoptosis. These compounds have specific action and minimal cytotoxicity against uninfected host cells. Additionally, combination treatment of artemisinin derivatives with the existing drug BPQ shows a synergistic effect.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Chemistry, Analytical
Xin Peng, Jiao Yang, Wenbin Liang, Yudong Sun, Xuecui Mei, Guanghui Zhang, Ruo Yuan, Yingchun Li
Summary: A DNA molecular tool based on the DL hairpins mediated D-HCR system is proposed for producing intact G-quadruplex aptamers. The system produces double-stranded and single-stranded DNA nanowires through hairpin strand displacement events, resulting in the release of InG4 aptamers. This system provides a flexible method for preparing nanowires and demonstrates high sensitivity in fluorescence and colorimetric biosensors.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yun Lei, Kun Wang, Jia-Yong Yang, Xin-Hua Lin, Ai-Lin Liu
Summary: A new electrochemical DNA biosensor based on double-probe mode and enzyme-mediated multiple signal electrocatalysis is developed for highly sensitive detection of double-stranded PML/RAR alpha fusion gene. The combination of dual-probe mode, multiple signal amplification strategy, and electrocatalytic activity of enzyme brings significant improvement in the electrochemical sensing performance.
ANALYTICA CHIMICA ACTA
(2022)
Article
Obstetrics & Gynecology
Wei Le, Fang Zhou, Jun Xiang, Yiming Weng, Denglong Wu, Jun Xu, Jinfu Zhang
Summary: This study reveals the specific mechanism of 53BP1 in the DNA damage repair process of germline stem cells, providing new theoretical basis and insights for the diagnosis and treatment of male infertility.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Meng Li, Betty C. A. M. van Esch, Paul A. J. Henricks, Johan Garssen, Gert Folkerts
Summary: The inhibitory effects of butyrate and propionate on TNF alpha-induced IL-8 production were mediated by the HDACs/IL-33/NF-kappa B pathway, while their effects on VCAM-1 expression might be associated with the HDACs/MAPK signaling pathway, independently of IL-33.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
James J. W. Hucklesby, Akshata Anchan, Simon J. O'Carroll, Charles P. Unsworth, E. Scott Graham, Catherine E. Angel
Summary: Commercially available instruments ECIS, xCELLigence, and cellZscope can measure the impedance of cellular monolayers, with ECIS showing the highest sensitivity and resolution when compared simultaneously.
Article
Biochemistry & Molecular Biology
Ji Tae Kim, Dana L. Napier, Jinhwan Kim, Chang Li, Eun Y. Lee, Heidi L. Weiss, Qingding Wang, B. Mark Evers
Summary: The disturbance of self-regeneration in mammalian intestinal epithelium is associated with intestinal disorders, particularly inflammatory bowel diseases (IBDs). TNF alpha inhibits ketone body production, but the exact role of ketogenesis in TNF alpha-mediated pathology is not fully understood. The study showed that HMGCS2, a key enzyme in ketogenesis, is downregulated by TNF alpha and plays a role in attenuating apoptosis and inflammation in intestinal epithelial cells.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Chemistry, Analytical
Neng Ling, Huiming Liu, Junxiao Guo, Zhouliang Liang, Yibin Zhang, Hui Li, Hui Wu, Tiantian Xie, Yijun Yuan, Xiahui Li, Menglan Peng, Xianhua Wei, Long Liang, Jing Liu, Wencan Wu, Mao Ye
Summary: In this study, a DNA aptamer expression system was developed to generate DNA aptamers with functional activity in mammalian cells by mimicking retroviruses. DNA aptamers targeting intracellular Ras and membrane-bound CD71 were successfully generated in cells. The expressed Ras aptamer not only specifically bound to the intracellular Ras protein but also inhibited the phosphorylation of downstream ERK1/2 and AKT. Furthermore, by inserting the DNA aptamer expression system into a lentivirus vector, stable production of the aptamer in cells resulted in the inhibition of lung cancer cell proliferation.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Binhui Zhou, Wenyi Yang, Wushan Li, Le He, Liaoxun Lu, Lichen Zhang, Zhuangzhuang Liu, Ying Wang, Tianzhu Chao, Rong Huang, Yanrong Gu, Tingting Jia, Qiaoli Liu, Shuanghua Tian, Philippe Pierre, Takahiro Maeda, Yinming Liang, Eryan Kong
Summary: Zdhhc2 plays a crucial role in controlling inflammatory response in psoriasis by regulating interferon-alpha production, and its deficiency leads to reduced inflammation and pDC activation in murine skin. This study sheds light on the potential of targeting Zdhhc2 as a therapeutic approach for inflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Engineering, Environmental
Yuanfeng Pang, Qing Li, Chongwen Wang, Shuai Zhen, Zhiwei Sun, Rui Xiao
Summary: By combining CRISPR-Cas12a and SERS technology, the sensitivity and specificity of LFA nucleic acid detection have been improved, allowing for direct quantification of HIV-1 dsDNA in less than 1 hour and recognition of HIV-1 single-base drug resistance mutations. This paper-based CRISPR-SERS strip has great potential for point-of-care testing in resource-poor or non-laboratory environments.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Young Bok (Abraham) Kang, Jinsu Eo, Beyza Bulutoglu, Martin L. Yarmush, O. Berk Usta
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biology
Haishui Huang, Camilo Rey-Bedon, Martin L. Yarmush, O. Berk Usta
Article
Biotechnology & Applied Microbiology
Beyza Bulutoglu, Julie Devalliere, Sarah L. Deng, Aylin Acun, Sarah S. Kelangi, Basak E. Uygun, Martin L. Yarmush
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Multidisciplinary Sciences
Jay Luther, Sanjoy Khan, Manish K. Gala, Dmitry Kedrin, Gautham Sridharan, Russell P. Goodman, John J. Garber, Ricard Masia, Erik Diagacomo, Daniel Adams, Kevin R. King, Samuel Piaker, Hans-Christian Reinecker, Martin L. Yarmush, Josepmaria Argemi, Ramon Bataller, Jules L. Dienstag, Raymond T. Chung, Suraj J. Patel
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Cell Biology
Paulina Krzyszczyk, Hwan June Kang, Suneel Kumar, Yixin Meng, Maurice D. O'Reggio, Kishan Patel, Ivan S. Pires, Martin L. Yarmush, Rene S. Schloss, Andre F. Palmer, Francois Berthiaume
WOUND REPAIR AND REGENERATION
(2020)
Article
Medicine, General & Internal
Anna Zhang, Cailah Carroll, Siavash Raigani, Negin Karimian, Viola Huang, Sonal Nagpal, Irene Beijert, Robert J. Porte, Martin Yarmush, Korkut Uygun, Heidi Yeh
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Biotechnology & Applied Microbiology
Ehab O. A. Hafiz, Beyza Bulutoglu, Soheir S. Mansy, Yibin Chen, Hoda Abu-Taleb, Somia A. M. Soliman, Ali A. F. El-Hindawi, Martin L. Yarmush, Basak E. Uygun
Summary: This study demonstrated the successful formation of transplantable hepatic microtissues with functional bile drainage, achieved through the coculturing of rat primary hepatocytes, cholangiocytes, and stromal cells. The hepatocytes maintained viability and functionality within the spheroids for up to 7 days, showing potential for repopulating liver scaffolds for transplantable liver grafts.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Critical Care Medicine
Safak Mert, Beyza Bulutoglu, Christopher Chu, Maggie Dylewski, Florence M. Lin, Yong-Ming Yu, Martin L. Yarmush, Robert L. Sheridan, Korkut Uygun
Summary: This study observed significant metabolic and lipid changes in liver and muscle tissues of severe burn injury rats, indicating potential liver-muscle crosstalk during hypermetabolism, where the tissues may exacerbate each other's metabolic pathologies through excessive utilization of certain metabolites.
JOURNAL OF BURN CARE & RESEARCH
(2021)
Article
Dermatology
Mengjie Wu, Andrey Ethan Rubin, Tianhong Dai, Rene Schloss, Osman Berk Usta, Alexander Golberg, Martin Yarmush
Summary: The study optimized PEF parameters to achieve the best disinfection efficacy for Pseudomonas aeruginosa infection in a stable mouse burn wound model. Results showed that PEF treatment reduced bacterial load, decreased inflammatory signs, and modulated inflammatory responses in infected burn wounds, suggesting PEF treatment as a potent candidate for antimicrobial therapy for P. aeruginosa burn infections.
ADVANCES IN WOUND CARE
(2021)
Article
Engineering, Biomedical
Aylin Acun, Ruben Oganesyan, Korkut Uygun, Heidi Yeh, Martin L. Yarmush, Basak E. Uygun
Summary: The extracellular matrix of the liver changes with age, affecting cell function, and donor age should be considered an important factor for bioengineering liver substitutes.
Article
Chemistry, Multidisciplinary
Hwan June Kang, Suneel Kumar, Arielle D'Elia, Biraja Dash, Vikas Nanda, Henry C. Hsia, Martin L. Yarmush, Francois Berthiaume
Summary: The combination of AGEs and RAGE triggers pro-inflammatory signals that inhibit the proliferative phase of skin wound healing. A new coacervate system has been developed to reverse AGE-mediated changes, accelerate diabetic wound healing, and provide a potential therapeutic approach.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Biotechnology & Applied Microbiology
Anil B. Shrirao, Rene S. Schloss, Zachary Fritz, Mayur V. Shrirao, Robert Rosen, Martin L. Yarmush
Summary: Blood autofluorescence is explored as a label-free approach for detecting cell types and diseases. Despite its high sensitivity, it lacks disease specificity and is affected by various factors such as sample type and preparation.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Engineering, Biomedical
Haishui Huang, Xiaoming He, Martin L. Yarmush
Summary: This review summarizes the principles and procedures of the three classical core technologies for preserving live mammalian biospecimens and how their limitations are being addressed through a combination of microfabrication, nanofabrication, materials science, and thermal-fluid engineering. It also examines the classical methods for preservation of mammalian biospecimens and discusses the mechanisms of cryoinjury, as well as how the limitations of these methods are being addressed.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Chemistry, Medicinal
Zachary R. Fritz, Rene S. Schloss, Martin L. Yarmush, Lawrence J. Williams
Summary: A novel engineering strategy was presented to improve autoantibody detection by using peptide fragments derived from the parent antigen. The study focused on enhancing binding affinity between peptides and antibodies by designing different flanking regions, leading to significant improvements in binding affinity and limits of detection for certain peptides. The reevaluation of the epitope suggested improved binding energy calculations and experimental data agreement, showcasing the potential of the Hidden Symmetry Model for peptide design and analysis.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Engineering, Biomedical
Beyza Bulutoglu, Aylin Acun, Sarah L. Deng, Safak Mert, Elise Lupon, Alexandre G. Lellouch, Curtis L. Cetrulo, Basak E. Uygun, Martin L. Yarmush
Summary: Elastin-like peptides (ELPs) are a versatile platform for tissue engineering and drug delivery. In this study, therapeutic molecules are genetically fused to ELPs and applied to wound healing. The results show that the fused molecules retain their bioactivity and the application of these ELP nanoparticles significantly promotes wound closure in diabetic mice.
ADVANCED HEALTHCARE MATERIALS
(2022)