Article
Biochemistry & Molecular Biology
Zhilong Ren, Duilio Michele Potenza, Yiqiong Ma, Guillaume Ajalbert, David Hoogewijs, Xiu-Fen Ming, Zhihong Yang
Summary: Hypoxia causes injury to podocytes, and the presence of Arg-II in podocytes contributes to this injury. However, the elimination of Arg-II does not significantly affect hypoxia-induced albuminuria, but it does reduce albuminuria in aging.
Article
Oncology
Francisca Valenzuela-Molina, Florina I. Bura, Mari C. Vazquez-Borrego, Melissa Granados-Rodriguez, Blanca Rufian-Andujar, Sebastian Rufian-Pena, Angela Casado-Adam, Juan Manuel Sanchez-Hidalgo, Lidia Rodriguez-Ortiz, Rosa Ortega-Salas, Ana Martinez-Lopez, Carmen Michan, Jose Alhama, Alvaro Arjona-Sanchez, Antonio Romero-Ruiz
Summary: This study demonstrated for the first time the presence of low intratumoral oxygen levels and increased markers of hypoxia and oxidative stress in PMP patients. However, more research is needed to understand the potential role of oxidative stress in PMP and develop new therapeutic strategies.
FRONTIERS IN ONCOLOGY
(2023)
Review
Pharmacology & Pharmacy
Yijie Wang, Xiong Liu, Weixiao Huang, Junjie Liang, Yan Chen
Summary: Alterations in protein ubiquitination and HIF signaling contribute to tumorigenesis and tumor progression. ROS upregulation in tumors has multiple effects on HIF signaling and the ubiquitin system. Multiple E3 ligases and UBDs work together to regulate the expression and activity of HIF, helping cancer cells cope with hypoxia. Understanding the complex networks between E3 ligase, UBDs, ROS, and HIF provides insights into the cellular response to hypoxia and identifies novel molecular targets for cancer treatment.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Endocrinology & Metabolism
Theresa Quinting, Anna Katharina Heymann, Anne Bicker, Theresa Nauth, Andre Bernardini, Thomas Hankeln, Joachim Fandrey, Timm Schreiber
Summary: Myoglobin has been found to play a significant role in breast cancer cells, potentially impacting cell survival and serving as a valuable target for cancer therapy.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Immunology
Robson Xavier Faria
Summary: The inflammatory focus and tumor microenvironment share similarities in terms of immune cells and macrophages. Under simulated hypoxic conditions, the function of P2X7 receptors is enhanced, along with increased HIF-1 alpha levels and suppressed HIF-1 alpha antagonists. Additionally, the intracellular P2X7 receptor regulator PIP2 is activated in simulated hypoxic conditions.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Keppner, Darko Maric, Ilaria Maria Christina Orlando, Laurent Falquet, Edith Hummler, David Hoogewijs
Summary: The kidney is highly dependent on a continuous oxygen supply and is sensitive to hypoxia. In this study, the authors investigated the response of cortical collecting duct cells to hypoxia using a mouse cell line. They found that hypoxia led to significant changes in gene expression, with decreased oxygen-linked processes and increased pathways associated with hypoxic responses. Knockdown experiments revealed the involvement of certain genes in the hypoxic induction of other genes. Overall, the study suggests that the mCCD(cl1) cell line is a suitable model for studying the cellular response to hypoxia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Cheng Gao, Cheryl H. T. Kwong, Qingfu Wang, Hiotong Kam, Jianwen Wei, Qian Chen, Jian Zhang, Simon M. Y. Lee, Dayong Gu, Ruibing Wang
Summary: A study found that many chemotherapeutic and immunotherapeutic agents are less effective in a hypoxic solid tumor. Current methods of oxygen delivery are limited, but using macrophage membrane coated chlorella can provide sustained oxygen and improve tumor hypoxia, enhancing the effectiveness of chemotherapy and immunotherapy.
Article
Biochemistry & Molecular Biology
Ricardo Usategui-Martin, Ricardo Rigual, Marta Ruiz-Mambrilla, Jose-Maria Fernandez-Gomez, Antonio Duenas, Jose Luis Perez-Castrillon
Summary: Bone is crucial for support, protection, and mineral storage. It is continuously renewed to maintain healthy functioning. Hypoxia can negatively affect bone health by inhibiting bone formation and increasing bone resorption.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biology
Michael Ohh, Cassandra C. Taber, Fraser G. Ferens, Daniel Tarade
Summary: von Hippel-Lindau (VHL) disease is a rare hereditary cancer syndrome caused by mutations in pVHL and disruption of HIF alpha degradation, and the understanding of its genotype-phenotype relationship is crucial for future management of VHL patients.
Article
Biochemistry & Molecular Biology
Elena Monti, Alessandro Mancini, Emanuela Marras, Marzia Bruna Gariboldi
Summary: Experimental evidence suggests that mitochondrial ROS plays a pivotal role in hypoxia-induced Epithelial-Mesenchymal Transition (EMT), and inhibiting mitochondrial ROS production could reverse EMT. UQCRB is a key factor in hypoxic signaling-induced EMT and may serve as a new therapeutic target for drug development.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Review
Cell Biology
Scott S. Hannah, Sonyia McFadden, Andrea McNeilly, Conor McClean
Summary: Hypoxia can have significant impacts on bone health, with bone cells exhibiting unique responses to hypoxic stimuli. Changes in bone metabolism induced by hypoxia may be mediated through multiple mechanisms.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Immunology
Mengqing Xiong, Yang Zhao, Huaheng Mo, Haizhen Yang, Fang Yue, Ke Hu
Summary: The study revealed that intermittent hypoxia exacerbated BLM-induced pulmonary fibrosis, with higher expression of ROS and HIF-1 alpha related to oxidative stress and inflammation. There was a positive linear correlation between HIF-1 alpha expression and hydroxyproline content, as well as a positive correlation between inflammation and ROS expression.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Oncology
Junling Chen, Minglin Zhang, Zhiyuan Ma, Dumin Yuan, Jiaxing Zhu, Biguang Tuo, Taolang Li, Xuemei Liu
Summary: Hypoxia plays a significant role in the development of gastric cancer, while ion channels/transporters (ICTs) also have an important impact on regulating tumor cell activities. Targeting the hypoxic microenvironment and ICTs may be a promising strategy for the treatment of gastric cancer.
Review
Biochemistry & Molecular Biology
Diego Rodriguez, Deepika Watts, Diana Gaete, Sundary Sormendi, Ben Wielockx
Summary: The vascular network is crucial for sensing oxygen levels and hypoxia triggers a cascade of cellular signals, including angiogenesis, regulated by transcription factors such as HIFs and oxygen sensors like PHDs. Understanding how vascular cells respond to hypoxia under varying conditions is important for potential therapeutic targets in vascular dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Akram Hamidi, Alexandra Wolf, Rositsa Dueva, Melanie Kaufmann, Kirsten Goepelt, George Iliakis, Eric Metzen
Summary: This study established an in vitro model of murine hepatocyte derived cells and analyzed the role of HIF-1 alpha in apoptosis induction, DNA damage repair, and sensitivity to ionizing radiation. The results showed that HIF-1 alpha deficiency increased radiation sensitivity and affected DNA repair.
Article
Biotechnology & Applied Microbiology
Ehab O. A. Hafiz, Beyza Bulutoglu, Soheir S. Mansy, Yibin Chen, Hoda Abu-Taleb, Somia A. M. Soliman, Ali A. F. El-Hindawi, Martin L. Yarmush, Basak E. Uygun
Summary: This study demonstrated the successful formation of transplantable hepatic microtissues with functional bile drainage, achieved through the coculturing of rat primary hepatocytes, cholangiocytes, and stromal cells. The hepatocytes maintained viability and functionality within the spheroids for up to 7 days, showing potential for repopulating liver scaffolds for transplantable liver grafts.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Dermatology
Mengjie Wu, Andrey Ethan Rubin, Tianhong Dai, Rene Schloss, Osman Berk Usta, Alexander Golberg, Martin Yarmush
Summary: The study optimized PEF parameters to achieve the best disinfection efficacy for Pseudomonas aeruginosa infection in a stable mouse burn wound model. Results showed that PEF treatment reduced bacterial load, decreased inflammatory signs, and modulated inflammatory responses in infected burn wounds, suggesting PEF treatment as a potent candidate for antimicrobial therapy for P. aeruginosa burn infections.
ADVANCES IN WOUND CARE
(2021)
Article
Chemistry, Multidisciplinary
Hwan June Kang, Suneel Kumar, Arielle D'Elia, Biraja Dash, Vikas Nanda, Henry C. Hsia, Martin L. Yarmush, Francois Berthiaume
Summary: The combination of AGEs and RAGE triggers pro-inflammatory signals that inhibit the proliferative phase of skin wound healing. A new coacervate system has been developed to reverse AGE-mediated changes, accelerate diabetic wound healing, and provide a potential therapeutic approach.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Biotechnology & Applied Microbiology
Anil B. Shrirao, Rene S. Schloss, Zachary Fritz, Mayur V. Shrirao, Robert Rosen, Martin L. Yarmush
Summary: Blood autofluorescence is explored as a label-free approach for detecting cell types and diseases. Despite its high sensitivity, it lacks disease specificity and is affected by various factors such as sample type and preparation.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Engineering, Biomedical
Haishui Huang, Xiaoming He, Martin L. Yarmush
Summary: This review summarizes the principles and procedures of the three classical core technologies for preserving live mammalian biospecimens and how their limitations are being addressed through a combination of microfabrication, nanofabrication, materials science, and thermal-fluid engineering. It also examines the classical methods for preservation of mammalian biospecimens and discusses the mechanisms of cryoinjury, as well as how the limitations of these methods are being addressed.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Medicine, Research & Experimental
Camilo Rey-Bedon, Peony Banik, Aslihan Gokaltun, O. Hofheinz, Martin. L. Yarmush, M. Korkut Uygun, O. Berk Usta
Summary: This study investigates the impact of fatty liver on drug-drug interactions. Dysregulation of enzyme systems observed in fatty liver may increase the risk of unexpected drug interactions. An in vitro model was developed to study enzyme regulation in fatty liver, and it was found that fatty liver might exacerbate drug interactions related to CYP2B6 and CYP2C9 enzymes.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Chemistry, Medicinal
Zachary R. Fritz, Rene S. Schloss, Martin L. Yarmush, Lawrence J. Williams
Summary: A novel engineering strategy was presented to improve autoantibody detection by using peptide fragments derived from the parent antigen. The study focused on enhancing binding affinity between peptides and antibodies by designing different flanking regions, leading to significant improvements in binding affinity and limits of detection for certain peptides. The reevaluation of the epitope suggested improved binding energy calculations and experimental data agreement, showcasing the potential of the Hidden Symmetry Model for peptide design and analysis.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Review
Pharmacology & Pharmacy
Patrick G. Holder, Shion A. Lim, Christine S. Huang, Preeti Sharma, Yavuz S. Dagdas, Beyza Bulutoglu, Jonathan T. Sockolosky
Summary: Cytokines are potent immunoregulatory proteins that play important roles in cancer and have the potential for cancer immunotherapy. However, the use of cytokines as therapeutics has been limited by their complex biology and toxicities. Recent advances in immune checkpoint inhibitors and combination immunotherapies have reinvigorated interest in cytokines as therapeutics.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Engineering, Biomedical
Beyza Bulutoglu, Aylin Acun, Sarah L. Deng, Safak Mert, Elise Lupon, Alexandre G. Lellouch, Curtis L. Cetrulo, Basak E. Uygun, Martin L. Yarmush
Summary: Elastin-like peptides (ELPs) are a versatile platform for tissue engineering and drug delivery. In this study, therapeutic molecules are genetically fused to ELPs and applied to wound healing. The results show that the fused molecules retain their bioactivity and the application of these ELP nanoparticles significantly promotes wound closure in diabetic mice.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Engineering, Biomedical
A. Aslihan Gokaltun, Letao Fan, Luca Mazzaferro, Delaney Byrne, Martin L. Yarmush, Tianhong Dai, Ayse Asatekin, O. Berk Usta
Summary: This study developed a novel biocompatible, self-healable, and on-demand dissoluble supramolecular hybrid hydrogel burn dressing. By leveraging the interactions of a custom-designed cationic copolymer with cucurbit[7]uril and electrostatic interactions with clay nanosheets coated with an anionic polymer, the dressing achieved enhanced mechanical properties and fast on-demand dissolution. The dressing showed high mechanical strength (>50 kPa), rapid self-healing (within 1 minute), and quick dissolution (4-6 minutes) using an amantadine hydrochloride solution. It did not have any adverse effects on human dermal fibroblasts or epidermal keratinocytes in vitro and did not elicit any immune or inflammatory response in vivo murine experiments.
BIOACTIVE MATERIALS
(2023)
Review
Cell & Tissue Engineering
Annika Johnson, Samuel Reimer, Ryan Childres, Grace Cupp, Tia C. L. Kohs, Owen J. T. McCarty, Youngbok (Abraham) Kang
Summary: The tumor microenvironment (TME) is crucial in tumor development and drug resistance, but its mechanisms are elusive. In vitro microfluidic models called tumor chips have been developed to study the complex TME. Tumor chips allow real-time observation, precise control of microenvironment factors, and application of relevant stresses and perturbations. Their applications include drug screening, drug delivery assessment, and studies of immune cell and circulating tumor cell interactions. However, challenges remain in recapitulating tumor physiology. Overcoming these challenges could provide powerful testing platforms in cancer research.
CELLULAR AND MOLECULAR BIOENGINEERING
(2023)
Article
Chemistry, Analytical
Ekansh Mittal, Grace Cupp, Youngbok (Abraham) Kang
Summary: The imbalance of gut microbiome plays a crucial role in the progression of diseases, including cancer, due to increased inflammation in the body. To investigate the effect of gut microbiome-induced inflammation on cancer cells, a microfluidic chip was developed to simulate their interaction in vitro.
Article
Materials Science, Biomaterials
A. Aslihan Gokaltun, Luca Mazzaferro, Martin L. Yarmush, O. Berk Usta, Ayse Asatekin
Summary: This study introduces a novel, simple, fast, and scalable method for enhancing PDMS surface hydrophilicity and preventing nonspecific adsorption by incorporating a surface-segregating zwitterionic copolymer during fabrication.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Engineering, Biomedical
Annika Johnson, Grace Cupp, Nicholas Armour, Kyle Warren, Christopher Stone, Davin Lee, Nicholas Gilbert, Chris Hammond, John Moore, Youngbok (Abraham) Kang
Summary: To minimize the risks associated with cardiac catheterization, a anatomically and physiologically accurate cardiovascular flow simulator was developed for training medical professionals and testing medical devices. The simulator includes anatomical structures, different access options, simulates physiological pulsatile blood flow and temperature, serving as both an educational tool and a testing tool for design iteration.
FRONTIERS IN MEDICAL TECHNOLOGY
(2021)