Article
Multidisciplinary Sciences
Tomomitsu Doi, Hironori Hojo, Shinsuke Ohba, Kunie Obayashi, Motoyoshi Endo, Toshimasa Ishizaki, Akira Katoh, Hiroyuki Kouji
Summary: This study investigates the regulatory mechanisms underlying β-catenin coactivator usage in human pancreatic carcinoma cells. The results suggest that AP-1 may coordinate the usage of β-catenin coactivators in these cells.
Article
Oncology
Michael Bordonaro
Summary: Dietary fiber has a preventive effect against colorectal cancer due to the fermentation product butyrate inducing CRC cell growth arrest and apoptosis. The interaction of CBP and p300 with beta-catenin influences CRC cell physiology, with CBP-Wnt vs. p300-Wnt activity ratio impacting the outcomes. Sam68 and Pygo2 are hypothesized to be responsible for the cell type-specific response of CRC cell lines to ICG-001 and butyrate cotreatment.
Article
Multidisciplinary Sciences
Angelique N. Masibag, Christopher J. Bergin, Joshua R. Haebe, Aicha Zouggar, Muhammad S. Shah, Tamara Sandouka, Amanda Mendes da Silva, Francois M. Desrochers, Aube Fournier-Morin, Yannick D. Benoit
Summary: Cancer stem cells play a key role in tumorigenesis and therapy resistance, and disrupting CBP/beta-Catenin interactions with reverse-turn peptidomimetics shows promise in curbing hyperactive Wnt/beta-Catenin signaling in CSCs. Recent studies have identified Sam68 as a critical mediator of the response to these peptidomimetics in CSC populations. Through in silico drug discovery, YB-0158 was identified as a small molecule with enhanced translational potential in altering key hallmarks of human colorectal CSCs.
Article
Biochemical Research Methods
Jiong Yang, Shigang Ding
Summary: The challenge in colorectal cancer treatment is the lack of specific-targeting therapy to precisely kill malignant cells. A study designed an RNA-only delivery kill switch to eliminate CMS2 type CRC cells, showing a proof-of-principle approach for targeted therapy.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jantine van Voorden, Remco Keijser, Geertruda J. M. Veenboer, Solange A. Lopes Cardozo, Dina Diek, Jennifer A. Vlaardingerbroek, Marie van Dijk, Carrie Ris- Stalpers, Ans M. M. van Pelt, Gijs B. Afink
Summary: Early placenta development involves the differentiation of cytotrophoblast into extravillous and syncytiotrophoblast cells. Defective trophoblast development due to EP300 mutations can lead to severe pregnancy complications, such as fetal growth restriction and pre-eclampsia. In this study, the researchers investigated the role of EP300 and CREBBP in trophoblast differentiation using human trophoblast stem cells (TSCs) and organoids. They found that inhibiting EP300 or CREBBP blocked trophoblast differentiation, but specifically inhibiting EP300 resulted in complications similar to Rubinstein-Taybi syndrome pregnancies. Furthermore, they discovered that EP300 facilitates trophoblast differentiation by interfering with EGFR signaling. The findings suggest the crucial involvement of EP300 in early placentation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Lishan Fang, Dane Ford-Roshon, Max Russo, Casey O'Brien, Xiaozhe Xiong, Carino Gurjao, Maximilien Grandclaudon, Srivatsan Raghavan, Steven M. Corsello, Steven A. Carr, Namrata D. Udeshi, James Berstler, Ewa Sicinska, Kimmie Ng, Marios Giannakis
Summary: The RNF43 G659fs mutation is an oncogenic mutation in colorectal cancer and sensitizes tumor cells to PI3K/mTOR inhibition. PI3K/mTOR inhibitors demonstrate promising antitumor activity in cells and models carrying RNF43_p.G659fs mutations, possibly by regulating PI3K signaling and interferon response gene expression.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Jung Kyu Choi, Ihn-Sil Kwak, Sae-Bom Yoon, Heeyeong Cho, Byoung-San Moon
Summary: CPD0857 has been found to inhibit tumor stem cells in colorectal cancer, reducing proliferation and promoting differentiation, which may serve as a potential drug targeting CSCs.
Article
Chemistry, Multidisciplinary
Yanmei Zhang, Zixuan Wang, Qifan Hu, Hao Luo, Bingchuan Lu, Yunhe Gao, Zhi Qiao, Yongsen Zhou, Yongcong Fang, Jin Gu, Ting Zhang, Zhuo Xiong
Summary: This study identifies a new biomaterial and method for inducing and enriching colorectal cancer stem cells (CSCs) using three-dimensional bioprinting. The use of GelMA-nanoclay hydrogels promotes the formation of spheroids expressing stemness markers and enhances the self-renewal, differentiation, and tumorigenic capacity of cancer cells in vitro and in vivo. The study also demonstrates that spheroids from GelMA-nanoclay hydrogels have increased stemness and sensitivity to anti-CSC compounds compared to traditional methods. This research is significant for facilitating effective CSC-targeting drug screening.
Article
Genetics & Heredity
Carli M. King, Olivia M. Marx, Wei Ding, Walter A. Koltun, Gregory S. Yochum
Summary: Mutations in Wnt/beta-catenin signaling pathway components drive colorectal cancer by deregulating gene expression controlled by TCF family transcription factors. TCF7L1 plays a significant role in this process by repressing LGR5 expression through binding to a novel WRE at the LGR5 gene locus. This modulation governs spheroid formation potential of CRC cells.
Article
Oncology
Saira R. Ali, Ayla Orang, Shashikanth Marri, Ross A. McKinnon, Robyn Meech, Michael Z. Michael
Summary: High-fiber diets with butyrate can prevent colorectal cancer by modulating gene and microRNA expression, providing insights into potential novel drug targets for cancer prevention and treatment.
Article
Medicine, Research & Experimental
So-Yeon Park, Ji-Young Kim, Gyu-Beom Jang, Jang-Hyun Choi, Jee-Heun Kim, Choong-Jae Lee, Sunjae Lee, Jeong-Heum Baek, Kwan-Kyu Park, Jin-Man Kim, Hee Jin Chang, Nam-Chul Cho, Jeong-Seok Nam
Summary: This study identified an increased expression of CD45 in CRC (EpCAM+) epithelial cells after CRT treatment. High CD45 expression promoted CRC cell survival upon 5-fluorouracil or radiation treatment, while CD45 depletion sensitized CRC cells to CRT. CD45 was found to be preferentially expressed in cancer stem-like cells (CSCs) and played a crucial role in CSC functions such as cancer initiation, repopulation, and metastasis.
Review
Biochemistry & Molecular Biology
Silvia Anna Ciafre, Monia Russo, Alessandro Michienzi, Silvia Galardi
Summary: Long noncoding RNAs (lncRNAs) play a role in the initiating cells of various tumors and have different mechanisms that affect tumor onset and progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Kesara Nittayaboon, Kittinun Leetanaporn, Surasak Sangkhathat, Sittirak Roytrakul, Raphatphorn Navakanitworakul
Summary: In this study, the cytotoxicity of metformin (MET) on butyrate-resistant (BR) colorectal cancer (CRC) cells was investigated using a 3D spheroid culture model. The results showed that MET reduced spheroid size, migration, and viability, while increasing spheroid death. The molecular mechanism involved the upregulation of AMPK and Akt, and the downregulation of ACC and mTOR, leading to caspase activation and apoptosis.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Developmental Biology
Clara R. Stelman, Britessia M. Smith, Bidushi Chandra, Rachel H. Roberts-Galbraith
Summary: The freshwater flatworm Schmidtea mediterranea is a valuable model for studying adult pluripotent stem cells, particularly in the context of robust whole-body regeneration. Screening planarian homologs of genes regulating chromatin biology identified six genes with novel functions in planarian homeostasis, regeneration, and behavior.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Xing Kang, Changan Liu, Yanqiang Ding, Yunbi Ni, Fenfen Ji, Harry Cheuk Hay Lau, Lanping Jiang, Joseph J. Y. Sung, Sunny H. Wong, Jun Yu
Summary: This study found that the probiotic species R. intestinalis has a protective effect against colorectal tumourigenesis by producing butyrate, and it can also enhance the efficacy of anti-PD-1 treatment. These findings provide evidence for the potential use of R. intestinalis as an adjuvant therapy for colorectal cancer.