Article
Agricultural Engineering
Endang Rahmat, Denis Okello, Hyoseon Kim, Jun Lee, Yuseong Chung, Richard Komakech, Youngmin Kang
Summary: The study optimized cultivation conditions and scaled up production using a bioreactor for Rehmannia glutinosa roots, resulting in improved quality, biomass, acteoside content, and antioxidant activity. This approach can meet the demands of pharmaceutical and oriental medicine industries without impacting the plant's natural availability.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Nisar Ahmad, Palwasha Khan, Abdullah Khan, Maliha Usman, Mohammad Ali, Hina Fazal, Durrishahwar, Muhammad Nazir Uddin, Christophe Hano, Bilal Haider Abbasi
Summary: This study investigated the effect of Cuscuta reflexa extract as a biotic elicitor on the biomass accumulation and production of antioxidant secondary metabolites in submerged adventitious root cultures of Stevia rebaudiana. The results showed that Cuscuta reflexa extract moderately improved biomass accumulation and enhanced the synthesis of phenolics, flavonoids, and antioxidant activities.
Article
Biochemistry & Molecular Biology
Redouan Elboutachfaiti, Roland Molinie, David Mathiron, Yannis Maillot, Jean-Xavier Fontaine, Serge Pilard, Anthony Quero, Clement Brasselet, Marguerite Dols-Lafargue, Cedric Delattre, Emmanuel Petit
Summary: In this study, it was found that high dose treatment of COS oligosaccharides can indirectly trigger metabolic response modifications in flax plants, especially inducing the accumulation of (neo)lignans in the roots and flavones luteolin C-glycosides, and chlorogenic acid in the shoots.
Article
Biotechnology & Applied Microbiology
Diana Kreitmayer, Srikanth R. Gopireddy, Yuichi Aki, Koichi Nonaka, Nora A. Urbanetz, Eva Gutheil
Summary: Cell culture scale-up is a challenging task due to the simultaneous change of multiple hydrodynamic process characteristics. Different scale-up criteria were evaluated, and the most appropriate criterion was the average strain rate in the impeller zone. Successful scale-up was confirmed through cell culture experiments using Chinese Hamster Ovary cell cultivation.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Plant Sciences
Ji-Hye Kim, Jong-Eun Han, Hosakatte Niranjana Murthy, Ja-Young Kim, Mi-Jin Kim, Taek-Kyu Jeong, So-Young Park
Summary: Sageretia thea was cultivated with the aim of increasing the production of phenolic compounds in plant cell suspension cultures. Optimum callus was induced and methyl jasmonate was found to be a suitable elicitor for phenolic accumulation in cell suspensions. The use of balloon-type bioreactors resulted in the highest biomass yields after four weeks of cultivation.
Review
Plant Sciences
Soo-In Sohn, Subramani Pandian, Kasinathan Rakkammal, Muthiah Joe Virgin Largia, Senthil Kumar Thamilarasan, Sekaran Balaji, Yedomon Ange Bovys Zoclanclounon, Jayabalan Shilpha, Manikandan Ramesh
Summary: Secondary metabolites are important specialized molecules with proven health-promoting effects on humans. Exogenous jasmonates (JAs) have been recognized for their ability to trigger plant growth and development and can also effectively elicit the synthesis of secondary metabolites. However, large-scale production of these metabolites is limited, but advancements in plant cell culture technology and synthetic biology offer potential avenues for overcoming this limitation.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Mubashra Inam, Iqra Attique, Maryam Zahra, Amna Komal Khan, Mariam Hahim, Christophe Hano, Sumaira Anjum
Summary: Secondary metabolites (SMs) with medicinal properties have high commercial value, but their production remains low. Nano-elicitation using metal oxide NPs (MONPs) has emerged as an effective strategy to enhance SMs production in plants. This review explores the different routes of exposure of MONPs in plants and their role as novel elicitors of important classes of SMs, as well as the underlying mechanism and uptake and translocation in plants. The review also addresses knowledge gaps and future research directions.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Agricultural Engineering
Maria Stasinska-Jakubas, Barbara Hawrylak-Nowak, Slawomir Dresler, Magdalena Wojciak, Katarzyna Rubinowska
Summary: Plant elicitors, such as chitosan lactate and salicylic acid, have been extensively used to increase the production of secondary metabolites in vitro. In vivo studies are still needed to fully understand the biological basis of elicitation. Among the compounds studied, chitosan lactate and salicylic acid showed the strongest elicitation effects on phenolic metabolites in lemon balm, while a mixture of elicitors did not significantly change metabolite levels. Additionally, the highest concentrations of metabolites were observed on the 8th day of elicitation, and the elicitors also increased antioxidant activity in plant extracts.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Biotechnology & Applied Microbiology
A. B. Cardillo, M. Perassolo, A. M. Giulietti, Julian Rodriguez Talou
Summary: Cyclodextrins trigger secondary metabolite production and release in plant cell cultures, forming complexes with bioactive compounds to reduce feedback inhibition and degradation. Plant cell culture is an alternative source for continuous and homogeneous production of secondary metabolites, overcoming intermittent supply from natural or agricultural sources. Elicitation with cyclodextrins can boost plant cell defense responses and accumulation of secondary metabolites, contributing to product removal and preventing degradation and toxicity.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biotechnology & Applied Microbiology
Magnus Ask, Stuart M. Stocks
Summary: This article presents an engineering approach to predicting evaporation rates in bioreactors based on the air concentration entering and leaving the bioreactor, with a focus on aeration rate and condenser temperature. Through experiments, it was found that aeration rate and condenser temperature were the main factors affecting evaporation, while other factors had minimal impact.
BIOTECHNOLOGY LETTERS
(2022)
Review
Biotechnology & Applied Microbiology
Rakesh Bhaskar, Louretta Spandhana Eruva Xavier, Giriprasad Udayakumaran, Dharani Shree Kumar, Rashmitha Venkatesh, Praveen Nagella
Summary: Plant secondary metabolites are naturally produced in response to environmental stress, and their production can be increased using plant tissue culture and elicitors. Bacterial, fungal, and algal elicitors have been shown to significantly enhance the production of secondary metabolites in plants.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Diksha Bagal, Aksar Ali Chowdhary, Shakti Mehrotra, Sonal Mishra, Sonica Rathore, Vikas Srivastava
Summary: Plants are important sources of secondary metabolites, but natural plant populations cannot meet the commercial demand due to low accumulation volume. Hairy root cultures induced by plant interaction with Rhizobium rhizogenes are a practical solution for producing valuable secondary metabolites at low cost and without seasonal variations. Metabolic engineering in hairy roots, including overexpression and silencing of genes and transcription factor overexpression, can enhance metabolite production. This review focuses on alkaloids and discusses future prospects for meeting production demands through metabolic engineering in hairy roots.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Victor Rezende Moreira, Thais Girardi Carpanez, Fabio Soares dos Santos, Leidiane Santana Santos, Dayane dos Santos Fernandes, Luzia Sergina Franca-Neta, Lisete Celina Lange, Miriam Cristina Santos Amaral
Summary: This study compared the performance of aerobic and anaerobic membrane bioreactors in vinasse recycling, with anaerobic system showing better removal efficiency for organic matter and aerobic system demonstrating better efficiency in total nitrogen removal.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Chemistry, Multidisciplinary
R. Jenifer Munoz-Gomez, Isabel Rivero-Cruz, Berenice Ovalle-Magallanes, Edelmira Linares, Robert Bye, Armando R. Tovar, Lilia G. Noriega, Claudia Tovar-Palacio, Rachel Mata
Summary: This study investigates the medicinal plant Peniocereus greggii roots' hypoglycemic and antihyperglycemic effects on treating diabetes. The active compounds found were a series of cholestane steroids and a new chemical entity. One of the compounds showed insulin sensitizer action. A quantification method for certain compounds in the crude drug was also developed and validated.
Article
Plant Sciences
Liulong Li, Xiaona Dong, Mengting He, Mei Huang, Jian Cai, Qin Zhou, Yingxin Zhong, Dong Jiang, Xiao Wang
Summary: Waterlogging priming is a promising technique for enhancing plant waterlogging tolerance. This study investigated the mechanisms behind root resistance in wheat under waterlogging stress and found that priming improved gas exchange, aerenchyma formation, and gene expression in adventitious roots, ultimately reducing the severity of damage caused by waterlogging.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Hosakatte Niranjana Murthy, Guggalada Govardhana Yadav, Sathish Shekhappa Kadapatti, M. Sandhya
Summary: The objective of this study was to evaluate the phytochemicals and antioxidant potential of Garcinia indica resin. Acetone and methanol extracts contain high amounts of phenolics and flavonoids, while methanol extract has the highest alkaloid content. GC-MS analysis identified terpenoids as the major compounds in acetone extract. Both acetone and methanol extracts showed excellent antioxidant activity. This study suggests that G. indica resin can be used for medicinal applications.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: This article introduces the major groups of medicinal plants in the Rubia genus, such as Rubia cordifolia, Rubia tinctorum, and Rubia akane. These plants contain anthraquinones (AQs), particularly alizarin and purpurin, which have various pharmacological effects. AQs have been used as natural dyes and color additives in industries. Different techniques and approaches, including cell and organ cultures, have been used to produce biomass and secondary metabolites from Rubia species. Bioreactor cultures have also been emphasized as a method to grow cells for AQ production.
Review
Biotechnology & Applied Microbiology
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: Since ancient times, Morinda species, especially Morinda citrifolia, have been used for therapeutic purposes. Various natural substances with bioactivity, such as iridoids, anthraquinones, coumarins, flavonoids, lignans, phytosterols, and carotenoids, have been identified. Among these, anthraquinone derivatives are the most important and are utilized as natural coloring agents with medicinal functions. This article provides a summary of the biotechnological methods used for the bioproduction of anthraquinone derivatives using cell and organ cultures of Morinda species. The production of these chemicals in bioreactor cultures is also discussed.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Serisha Gangaram, Yougasphree Naidoo, Yaser Hassan Dewir, Moganavelli Singh, Johnson Lin, Hosakatte Niranjana Murthy
Summary: This study characterized the phytochemical compounds and antibacterial efficacies of Barleria albostellata, a relatively understudied shrub in South Africa. The crude leaf and stem extracts exhibited antibacterial activity against various pathogenic microorganisms, and analysis revealed the presence of bioactive compounds with potential medicinal value. These findings highlight the potential of B. albostellata as a source for novel antibacterial agents.
Article
Plant Sciences
Ji-Hye Kim, Jong-Eun Han, Hosakatte Niranjana Murthy, Ja-Young Kim, Mi-Jin Kim, Taek-Kyu Jeong, So-Young Park
Summary: Sageretia thea was cultivated with the aim of increasing the production of phenolic compounds in plant cell suspension cultures. Optimum callus was induced and methyl jasmonate was found to be a suitable elicitor for phenolic accumulation in cell suspensions. The use of balloon-type bioreactors resulted in the highest biomass yields after four weeks of cultivation.
Article
Plant Sciences
Oluwatosin Temilade Adu, Yougasphree Naidoo, Johnson Lin, Depika Dwarka, John Mellem, Hosakatte Niranjana Murthy, Yaser Hassan Dewir
Summary: This study examined the nutritional properties and cytotoxic effects of D. villosa. The extracts and nanoparticles of D. villosa showed cytotoxic activity against cancerous cell lines, particularly MCF-7 and A549.
Article
Biology
Dayanand Dalawai, Hosakatte Niranjana Murthy, Yaser Hassan Dewir, Joseph Kadanthottu Sebastian, Anish Nag
Summary: This study determined the phytochemical composition and bioactive chemical components of Andrographis macrobotrys using GC-MS, and evaluated the antioxidant potential of its extracts. The results showed that the stem extract of the plant has a higher concentration of phenolics and exhibits strong antioxidant activity. These findings highlight the importance of A. macrobotrys as a medicinal plant and a potential source of antioxidants.
Article
Horticulture
Hosakatte Niranjana Murthy, Guggalada Govardhana Yadav, Sathish Shekhappa Kadapatti, Akarsha H. Pote, Ramalinga Jagali, Vidya Yarashi, Yaser Hassan Dewir
Summary: The present study investigated the nutritional composition, phytochemical composition, and antioxidant activities of Rourea minor fruits. The ripened seeds and pulp contained various components, including fat, protein, carbohydrates, ash, and fiber. Both the seeds and pulp were also rich in microelements. The study also found that the extracts from the fruits exhibited strong antioxidant activity. The findings suggest that Rourea minor fruits could be a valuable source of nutrients, microelements, and antioxidants.
Article
Horticulture
Yaser Hassan Dewir, Muhammad M. Habib, AbdulAziz A. AlQarawi, Thobayet S. Alshahrani, Ahmed Ali Alaizari, Jahangir A. Malik, Mona S. Alwahibi, Hosakatte Niranjana Murthy
Summary: This study examined the effect of arbuscular mycorrhizal fungi (AMF) on the growth and development of tissue-cultured Philodendron bipinnatifidum plantlets during the acclimatization phase. The results showed that AMF-treated plants had better growth characteristics and root development compared to non-AMF plants. These findings highlight the importance of mycorrhization in promoting vegetative growth and acclimatization of micropropagated plants.
Review
Biotechnology & Applied Microbiology
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: In vitro plant cell and organ cultures are attractive alternatives for producing valuable specialized metabolites. Adventitious roots are often superior to cell suspension cultures for accumulating high levels of specialized metabolites. Different bioreactor systems have been investigated for cultivation of adventitious roots.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Plant Sciences
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: Plant cells and organ cultures are viable alternatives for producing specialized metabolites. Elicitation is a strategy used to enhance the accumulation of these compounds, and nanoparticles have recently been used as unique elicitors. This review summarizes the applications and usage of nanoparticles as elicitors in plant cell and organ cultures.
PLANT GROWTH REGULATION
(2023)
Review
Plant Sciences
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Jong-Eun Hahn, Han-Sol Lee, Kee Yoeup Paek, So Young Park
Summary: This review provides an update on the use of somatic embryogenesis in the production of valuable secondary metabolites, discussing the factors influencing their generation, elicitation methods, and prospective applications.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2023)
Article
Agronomy
Myeong-Jin Lim, Hosakatte Niranjana Murthy, Hyun-Young Song, Su-Young Lee, So-Young Park
Summary: This study investigated the effects of red, blue, white, and red plus blue and red, blue, and green LEDs on the in vitro regeneration of Gerbera jamesonii cv. 'Shy Pink'. The results showed that red plus blue LEDs had the greatest impact on shoot regeneration, growth, and root regeneration. Red and blue LEDs affected different parts of the plant, with blue LEDs promoting higher photosynthetic pigment content. White LEDs had a strong effect on chlorophyll fluorescence.
Article
Business
V. Mamtha, H. N. Narasimha Murthy, V. Pujith Raj, Prashantha Tejas, C. S. Puneet, Achyutha Venugopal, Sham AanMankunipoyil, C. Manjunatha
Summary: This study investigates the synergistic effect of AgNP and MgO as nanofillers in electrospun Polyurethane. The results show that the addition of MgO improves the physical properties of the nanofibers, while the addition of AgNP enhances the antibacterial activity.
CLOTHING AND TEXTILES RESEARCH JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Sathish Shekhappa Kadapatti, Hosakatte Niranjana Murthy
Summary: A reliable In vitro micropropagation method was developed in Andrographis producta through direct organogenesis, which is helpful for the multiplication, conservation, and utilization of this plant.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2022)