Article
Medicine, Research & Experimental
Pablo Tebas, Julie K. Jadlowsky, Pamela A. Shaw, Lifeng Tian, Erin Esparza, Andrea L. Brennan, Sukyung Kim, Soe Yu Naing, Max W. Richardson, Ashley N. Vogel, Colby R. Maldini, Hong Kong, Xiaojun Liu, Simon F. Lacey, Anya M. Bauer, Felicity Mampe, Lee P. Richman, Gary Lee, Dale Ando, Bruce L. Levine, David L. Porter, Yangbing Zhao, Don L. Siegel, Katharine J. Bar, Carl H. June, James L. Riley
Summary: By infusing CCR5 gene-edited CD4(+) T cells, delay of HIV viral rebound and enhancement of HIV-specific immune responses can be achieved, aiding HIV cure strategies. However, only a minority of patients showed signs of viral replication control, accompanied by significant restoration of HIV-specific CD8(+) T cell responses.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Immunology
An-Liang Guo, Jin-Fang Zhao, Lin Gao, Hui-Huang Huang, Ji-Yuan Zhang, Chao Zhang, Jin-Wen Song, Ruo-Nan Xu, Xing Fan, Ming Shi, Yan-Mei Jiao, Fu-Sheng Wang
Summary: The study found that CD11c(+) CD8(+) T cells can effectively control viral replication during HIV-1 infection and have the potential to be used as an immunotherapeutic approach.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Catherine Riou, Elsa du Bruyn, Cari Stek, Remy Daroowala, Rene T. Goliath, Fatima Abrahams, Qonita Said-Hartley, Brian W. Allwood, Nei-Yuan Hsiao, Katalin A. Wilkinson, Cecilia S. Lindestam Arlehamn, Alessandro Sette, Sean Wasserman, Robert J. Wilkinson
Summary: This study found that the attributes of SARS-CoV-2-specific CD4(+) T cells were associated with the severity of COVID-19, with severe cases showing poor polyfunctional potential, reduced proliferation capacity, and enhanced HLA-DR expression. HIV-1 and TB coinfection can alter the SARS-CoV-2 T cell response, with HIV-1 causing CD4(+) T cell depletion and active TB leading to decreased polyfunctional capacity of SARS-CoV-2-specific CD4(+) T cells in COVID-19 patients. Moreover, COVID-19 patients displayed a reduced frequency of Mycobacterium tuberculosis-specific CD4(+) T cells, which may affect TB disease progression.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Multidisciplinary Sciences
Rita Al-kolla, Alba Grifoni, Shane Crotty, Alessandro Sette, Sara Gianella, Jennifer Dan
Summary: Reagents to monitor T cell responses to the entire HIV genome have been developed in this study, which are crucial for assessing HIV-specific T cell responses in natural infection and therapeutic and vaccine interventions. The results showed that the developed reagents can detect HIV-specific CD4(+) and CD8(+) T cell responses in individuals with HIV, but not in individuals without HIV.
Article
Medicine, Research & Experimental
Anju Bansal, Mika N. Gehre, Kai Qin, Sarah Sterrett, Ayub Ali, Ying Dang, Sojan Abraham, Margaret C. Costanzo, Leon A. Venegas, Jianming Tang, N. Manjunath, Mark A. Brockman, Otto O. Yang, June Kan-Mitchell, Paul A. Goepfert
Summary: The study reveals that HLA-E-restricted CD8(+) T cell responses play a crucial role in determining the immunodominance of CD8(+) T cell responses in HIV infection, shedding light on the immune mechanisms involved in HIV pathogenesis.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Immunology
David R. Collins, Jonathan M. Urbach, Zachary J. Racenet, Umar Arshad, Karen A. Power, Ruchi M. Newman, Geetha H. Mylvaganam, Ngoc L. Ly, Xiaodong Lian, Anna Rull, Yelizaveta Rassadkina, Adrienne G. Yanez, Michael J. Peluso, Steven G. Deeks, Francesc Vidal, Mathias Lichterfeld, Xu G. Yu, Gaurav D. Gaiha, Todd M. Allen, Bruce D. Walker
Summary: Selective functional impairment of HIV-specific CD8(+) T cells may be prognostic of aborted HIV control.
Article
Immunology
Mauro Di Pilato, Miguel Palomino-Segura, Ernesto Mejias-Perez, Carmen E. Gomez, Andrea Rubio-Ponce, Rocco D'Antuono, Diego Ulisse Pizzagalli, Patricia Perez, Raphael Kfuri-Rubens, Alberto Benguria, Ana Dopazo, Ivan Ballesteros, Carlos Oscar S. Sorzano, Andres Hidalgo, Mariano Esteban, Santiago F. Gonzalez
Summary: This study reveals that N beta and N alpha neutrophils exhibit different migratory patterns and interactions with CD8 T cells in vivo following VACV infection. N beta neutrophils overexpress the alpha 4 beta 1 integrin compared to N alpha. Inhibiting alpha 4 beta 1 integrin can increase the N beta/N alpha ratio and enhance CD8 T-cell responses to HIV VACV-delivered antigens.
Article
Immunology
Mehwish Younas, Christina Psomas, Christelle Reynes, Renaud Cezar, Lucy Kundura, Pierre Portales, Corinne Merle, Nadine Atoui, Celine Fernandez, Vincent Le Moing, Claudine Barbuat, Albert Sotto, Robert Sabatier, Audrey Winter, Pascale Fabbro, Thierry Vincent, Jacques Reynes, Pierre Corbeau
Summary: This study found that some HIV-1-infected patients under efficient treatment display low-level viremia and unique features of immune activation distinct from microbial translocation. Patients with Profile E exhibited high levels of CD4+ T cell, monocyte, and endothelium activation, while other patients had different profiles of immune activation. These findings suggest a potential causal link between low-level viremia and atherosclerosis in virologic responders.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Tulio J. Lopera, Jorge A. Lujan, Eduardo Zurek, Wildeman Zapata, Juan C. Hernandez, Miguel A. Toro, Juan F. Alzate, Natalia A. Taborda, Maria T. Rugeles, Wbeimar Aguilar-Jimenez
Summary: Intestinal microbiota plays a crucial role in individuals with HIV infection and HIV-exposed individuals, influencing immune regulation. The microbiota profile of HIV-exposed individuals is similar to that of HIV-infected individuals, possibly due to cohabitation with their HIV+ partners.
Article
Immunology
Kristen W. Cohen, Yuan Tian, Casey Thayer, Aaron Seese, Robert Amezquita, M. Juliana McElrath, Stephen C. De Rosa, Raphael Gottardo
Summary: Ag-specific T cells, particularly polyfunctional CD4(+) T cells, are important for responding to viral infections. In this study, a novel live-cell multiplexed cytokine capture assay was developed to identify and profile vaccine-specific polyfunctional CD4(+) T cells. The researchers found that upregulated surface receptors (CD44 and CD82) and a Th2-biased transcriptional signature (IL-4, IL-5, and IL-13) were associated with reduced risk of HIV infection. By linking single-cell transcriptional and functional profiles, this study has provided insights into the potential contributions of polyfunctional T cells to vaccine-elicited immunity.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Min Guan, Laura Lim, Leo Holguin, Tianxu Han, Vibhuti Vyas, Ryan Urak, Aaron Miller, Diana L. Browning, Liliana Echavarria, Shasha Li, Shirley Li, Wen-Chung Chang, Tristan Scott, Paul Yazaki, Kevin Morris, Angelo A. Cardoso, M. Suzette Blanchard, Virginia Le Verche, Stephen J. Forman, John A. Zaia, John C. Burnett, Xiuli Wang
Summary: Stimulating CMV-specific T cells expressing HIV-specific CARs using a CMV vaccine can enhance T cell activation and persistence in HIV patients. Results from experiments show that CMV vaccination during ART can accelerate the expansion of CMV-HIV CAR T cells and improve control over HIV.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Medicine, Research & Experimental
Mengyan Li, Scott J. Garforth, Kaitlyn E. O'Connor, Hang Su, Danica M. Lee, Alev Celikgil, Rodolfo J. Chaparro, Ronald D. Seidel, R. Brad Jones, Ravit Arav-Boger, Steven C. Almo, Harris Goldstein
Summary: In this study, synTacs were used to expand highly functional HIV- and CMV-specific CD8(+) T cells, which showed potent antiviral activities in vivo. The results suggest that synTac-based approaches could be a promising therapeutic strategy for HIV cure and treatment of CMV and other viral infections.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Immunology
Vinicius A. Vieira, Jane Millar, Emily Adland, Maximilian Muenchhoff, Julia Roider, Claudia Fortuny Guash, Denise Peluso, Beatriz Thome, Maria C. Garcia-Guerrero, Mari C. Puertas, Alasdair Bamford, Christian Brander, Mary Carrington, Javier Martinez-Picado, John Frater, Gareth Tudor-Williams, Philip Goulder
Summary: This study analyzed the T-cell immunophenotype and HIV-specific response in pediatric elite controllers (PECs) and compared them to other adolescents. The results showed that PECs had lower immune activation levels and higher T-cell responses, which may contribute to viral control.
Article
Immunology
Chris Y. Chiu, Judy J. Chang, Ashanti Dantanarayana, Ajantha Solomon, Vanessa A. Evans, Rachel Pascoe, Celine Gubser, Lydie Trautman, Remi Fromentin, Nicolas Chomont, James H. McMahon, Paul U. Cameron, Thomas A. Rasmussen, Sharon R. Lewin
Summary: Blocking immune checkpoint (IC) proteins, particularly in combination with antibodies to LAG-3, CTLA-4, or TIGIT, shows potential in enhancing the elimination of HIV-infected cells and improving the activation and survival of immune cells in people with HIV on antiretroviral therapy (ART).
JOURNAL OF IMMUNOLOGY
(2022)
Article
Virology
Jernej Pusnik, Stephanie Fischinger, Ulf Dittmer, Stefan Esser, Marit J. van Gils, Rogier W. Sanders, Galit Alter, Hendrik Streeck
Summary: This study demonstrates that the generation of HIV-1 Env-specific antibodies facilitating antibody-dependent innate immune responses likely depends on Env-specific IL-21-secreting CD4(+)T and peripheral T follicular helper cells.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Madina Karimova, Niklas Beschorner, Werner Dammermann, Jan Chemnitz, Daniela Indenbirken, Jan-Hendrik Bockmann, Adam Grundhoff, Stefan Lueth, Frank Buchholz, Julian Schulze zur Wiesch, Joachim Hauber
SCIENTIFIC REPORTS
(2015)
Article
Biotechnology & Applied Microbiology
Janet Karpinski, Ilona Hauber, Jan Chemnitz, Carola Schaefer, Maciej Paszkowski-Rogacz, Deboyoti Chakraborty, Niklas Beschorner, Helga Hofmann-Sieber, Ulrike C. Lange, Adam Grundhoff, Karl Hackmann, Evelin Schrock, Josephine Abi-Ghanem, M. Teresa Pisabarro, Vineeth Surendranath, Axel Schambach, Christoph Lindner, Jan van Lunzen, Joachim Hauber, Frank Buchholz
NATURE BIOTECHNOLOGY
(2016)
Article
Multidisciplinary Sciences
Ana Lilia Martinez-Rocha, Mayada Woriedh, Jan Chemnitz, Peter Willingmann, Cathrin Kroeger, Birgit Hadeler, Joachim Hauber, Wilhelm Schaefer
SCIENTIFIC REPORTS
(2016)
Article
Biotechnology & Applied Microbiology
Ilona Hauber, Niklas Beschorner, Silke Schroedel, Jan Chemnitz, Nicolaus Kroeger, Joachim Hauber, Christian Thirion
HUMAN GENE THERAPY METHODS
(2018)
Editorial Material
Biochemical Research Methods
Annekatrin Richter, Jens Boch
Article
Biochemistry & Molecular Biology
Christina Ehlers, Susann Schirmer, Ralph H. Kehlenbach, Joachim Hauber, Jan Chemnitz
NUCLEIC ACIDS RESEARCH
(2013)
Article
Multidisciplinary Sciences
Dorothea Pieper, Susann Schirmer, Alexander T. Prechtel, Ralph H. Kehlenbach, Joachim Hauber, Jan Chemnitz
Article
Multidisciplinary Sciences
Annekatrin Richter, Jana Streubel, Christina Bluecher, Boris Szurek, Maik Reschke, Jan Grau, Jens Boch
NATURE COMMUNICATIONS
(2014)
Article
Microbiology
Ilona Hauber, Helga Hofmann-Sieber, Jan Chemnitz, Danilo Dubrau, Janet Chusainow, Rolf Stucka, Philip Hartjen, Axel Schambach, Patrick Ziegler, Karl Hackmann, Evelin Schroeck, Udo Schumacher, Christoph Lindner, Adam Grundhoff, Christopher Baum, Markus G. Manz, Frank Buchholz, Joachim Hauber
Article
Biochemistry & Molecular Biology
Jana Stundlova, Monika Hospodarska, Karolina Luksikova, Anna Volenikova, Tomas Pavlica, Marie Altmanova, Annekatrin Richter, Martin Reichard, Martina Dalikova, Sarka Pelikanova, Anatolie Marta, Sergey A. Simanovsky, Matyas Hirman, Marek Jankasek, Tomas Dvorak, Joerg Bohlen, Petr Rab, Christoph Englert, Petr Nguyen, Alexandr Sember
Summary: This study investigated the evolution of sex chromosomes in two sister species of African annual killifishes and found that both species shared homomorphic sex chromosomes that evolved prior to their divergence. The study also revealed differences in repetitive DNA sequences between the sex chromosomes, which may result from high turnover of repeat sequences and may not closely relate to the divergence inferred from earlier SNP analyses.
CHROMOSOME RESEARCH
(2022)
Article
Multidisciplinary Sciences
Jan Chemnitz, Dorothea Pieper, Lena Stich, Udo Schumacher, Stefan Balabanov, Michael Spohn, Adam Grundhoff, Alexander Steinkasserer, Joachim Hauber, Elisabeth Zinser
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Barbara Fries, Jochen Heukeshoven, Ilona Hauber, Cordula Gruettner, Carol Stocking, Ralph H. Kehlenbach, Joachim Hall, Jan Chemnitz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2007)
Article
Biochemistry & Molecular Biology
Sylvia Schuetz, Jan Chemnitz, Christiane Spillner, Marcus Frohme, Joachim Hauber, Ralph H. Kehlenbach
JOURNAL OF MOLECULAR BIOLOGY
(2006)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
