Article
Cell Biology
Melissa Klug, Kilian Kirmes, Jiaying Han, Olga Lazareva, Marc Rosenbaum, Giacomo Viggiani, Moritz von Scheidt, Juergen Ruland, Jan Baumbach, Gianluigi Condorelli, Karl-Ludwig Laugwitz, Markus List, Isabell Bernlochner, Dario Bongiovanni
Summary: Mass cytometry (CyTOF) is a new technology that enables high-resolution investigation of protein expression at the single cell level. This study provides a structured and reproducible method for staining platelets from platelet-rich plasma to study thrombocyte protein expression using CyTOF, allowing for sample storage and delayed measurement. The protocol also includes a bioinformatic pipeline for analyzing platelet expression, unlocking the potential of CyTOF analysis for studying platelet biology in health and disease.
Article
Hematology
Alessandro Aliotta, Debora Bertaggia Calderara, Maxime G. Zermatten, Lorenzo Alberio
Summary: The study demonstrated contrasting patterns in the intracellular ion concentrations of COAT-activated platelets compared to non-COAT platelets, with the former showing high and prolonged calcium concentration, transient sodium increase, and fast potassium efflux. Inhibition of the sodium-calcium exchanger (NCX) reduced calcium mobilization and prevented the formation of procoagulant platelets, highlighting the critical and dichotomous role of NCX during platelet activation induced by convulxin-plus-thrombin.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Matthew S. Hindle, Benjamin E. J. Spurgeon, Lih T. Cheah, Beth A. Webb, Khalid M. Naseem
Summary: The study identified novel platelet subpopulations that suggest differential regulation of platelet functions by PGI(2) using multicolor flow cytometry assays and computational analysis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Maria N. Barrachina, Lidia Hermida-Nogueira, Luis A. Moran, Vanessa Casas, Sarah M. Hicks, Aurelio M. Sueiro, Ying Di, Robert K. Andrews, Steve P. Watson, Elizabeth E. Gardiner, Joaquin Abian, Montserrat Carrascal, Maria Pardo, Angel Garcia
Summary: In this study, a comparative phosphoproteomic analysis of platelets from obese patients revealed up-regulated levels of phosphorylation events and altered signaling pathways compared to lean controls. Flow cytometry assays and mass spectrometry validation confirmed platelet dysfunction in obese individuals, including higher surface levels of GPVI and CLEC2. Soluble GPVI levels were also found to be elevated in plasma from obese patients, suggesting increased GPVI signaling activation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Cell Biology
Linda G. Rikkert, Frank A. W. Coumans, Chi M. Hau, Leon W. M. M. Terstappen, Rienk Nieuwland
Summary: A study developed a fast and simple single-step centrifugation protocol for producing high-quality platelet-free plasma for EV research. Compared to the traditional two-step protocol, the single-step protocol is faster, removes platelets effectively, and has a slightly higher plasma yield.
Article
Oncology
Anandi Krishnan, Sally Thomas
Summary: Advancements in next-generation techniques like RNA-sequencing have allowed for research into the transcriptome of anucleate blood platelets, revealing a rich platelet transcriptomic signature altered in response to disease. These platelet signatures capture information from parent cells and reflect influences of tumors, stroma, and vasculature. Despite potential for cancer diagnosis and prognosis, clinical translation of platelet transcriptomics remains limited.
BRITISH JOURNAL OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Sobha Karuthedom George, Lucia Laukova, Rene Weiss, Vladislav Semak, Birgit Fendl, Victor U. Weiss, Stephanie Steinberger, Guenter Allmaier, Carla Tripisciano, Viktoria Weber
Summary: This study demonstrates the differences in counting and characterizing platelet-derived EVs using different instruments and techniques. Flow cytometry and nanoparticle tracking analysis can accurately quantify the number of EVs, and reveal potential discrepancies in counts between different instruments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Dawid Polak, Marcin Talar, Nina Wolska, Dagmara W. Wojkowska, Kamil Karolczak, Karol Kramkowski, Tomasz A. Bonda, Cezary Watala, Tomasz Przygodzki
Summary: The A(2A) agonist HE-NECA in low doses in combination with inhibitors of P2Y(12) improved anti-thrombotic properties and was effective with chronic administration. Additionally, HE-NECA has hypotensive effects but does not significantly affect blood-brain barrier permeability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Physiology
Pamela Czajka, Alex Fitas, Daniel Jakubik, Ceren Eyileten, Aleksandra Gasecka, Zofia Wicik, Jolanta M. Siller-Matula, Krzysztof J. Filipiak, Marek Postula
Summary: MiRNAs play a crucial role in regulating platelet reactivity, serving as diagnostic and prognostic markers for platelet activity, as well as novel targets for cardiovascular disease therapy.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Karolina Siewiera, Magdalena Labieniec-Watala, Hassan Kassassir, Nina Wolska, Dawid Polak, Cezary Watala
Summary: The study found that prolonged exposure of blood platelets to high concentrations of glucose in diabetes can lead to increased mitochondrial respiration, but this is not the main reason for abnormal platelet functioning. Additionally, metformin was not able to inhibit platelet activation under in vitro conditions, indicating that its beneficial effect on the coagulation system in vivo may be related to other mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Pietro Minuz, Luca De Toni, Stefano Dall'Acqua, Andrea Di Nisio, Iva Sabovic, Marco Castelli, Alessandra Meneguzzi, Carlo Foresta
Summary: The experimental results demonstrate that exposure to C6O4 increases platelet response to agonists, potentially leading to an elevated risk of cardiovascular events. Acetylsalicylic acid (ASA) can partially inhibit this effect, indicating a possible prophylactic use.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Cell Biology
Anna Konstorum, Subhasis Mohanty, Yujiao Zhao, Anthony Melillo, Brent Vander Wyk, Allison Nelson, Sui Tsang, Tamara P. P. Blevins, Robert B. Belshe, Daniel G. G. Chawla, Matthew T. T. Rondina, Thomas M. M. Gill, Ruth R. R. Montgomery, Heather G. G. Allore, Steven H. H. Kleinstein, Albert C. C. Shaw
Summary: Platelets play important roles in both hemostasis and innate immunity. This study investigated how age and geriatric conditions affect platelet function during an immune response. The results showed age-associated changes in the platelet transcriptome and activation responses, suggesting a potential link between these alterations and age-related chronic inflammation and increased incidence of thrombotic and pro-inflammatory diseases in older adults.
Article
Medicine, General & Internal
Lydie Crescence, Markus Kramberg, Martine Baumann, Markus Rey, Sebastien Roux, Laurence Panicot-Dubois, Christophe Dubois, Markus A. Riederer
Summary: Selatogrel, a potent and reversible antagonist of the P2Y12 receptor, exhibited strong anti-thrombotic effects in animal models by inhibiting platelet function, preventing blood flow decrease, and dissolving pre-formed platelet thrombi without disrupting hemostatic seals. The findings suggest a potential dual benefit of selatogrel in patients with acute thrombosis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, General & Internal
August Wrotek, Oliwia Wrotek, Teresa Jackowska
Summary: This study aimed to analyze the prognostic value of platelet parameters in children hospitalized with laboratory-confirmed influenza. The study found that abnormalities in platelet count and mean platelet volume/platelet count ratio were associated with increased odds of complications and a more severe disease course.
Article
Biochemistry & Molecular Biology
Katrin Herken, Martin Glauner, Stefanie C. Robert, Matthias Maas, Sonja Zippel, Ulrike Nowak-Goettl, Barbara Zieger, Judith Lahav, Anke C. Fender, Kerstin Jurk, Beate E. Kehrel
Summary: The study found that neonatal platelets lack responsiveness to thrombospondin-1, resulting in a relatively impaired response to collagen. Platelet responsiveness varied with age, agonist, and activation marker among different pediatric age groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Justyna Rosinska, Joanna Maciejewska, Robert Narozny, Krystyna Osztynowicz, Beata Raczak, Slawomir Michalak, Cezary Watala, Wojciech Kozubski, Maria Lukasik
Article
Biochemistry & Molecular Biology
Nina Wolska, Magdalena Boncler, Dawid Polak, Joanna Wzorek, Tomasz Przygodzki, Magdalena Gapinska, Cezary Watala, Marcin Rozalski
Article
Multidisciplinary Sciences
Katarzyna Bialek, Piotr Czarny, Cezary Watala, Paulina Wigner, Monika Talarowska, Piotr Galecki, Janusz Szemraj, Tomasz Sliwinski
Article
Cell Biology
Hassan Kassassir, Kamil Karolczak, Karolina M. Siewiera, Dagmara W. Wojkowska, Marcin Braun, Cezary W. Watala
Article
Pharmacology & Pharmacy
Joanna Wzorek, Radoslaw Bednarek, Cezary Watala, Magdalena Boncler
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Tomasz Przygodzki, Boguslawa Luzak, Hassan Kassassir, Ewelina Mnich, Magdalena Boncler, Karolina Siewiera, Marcin Kosmalski, Jacek Szymanski, Cezary Watala
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Boguslawa Luzak, Magdalena Boncler, Marcin Kosmalski, Ewelina Mnich, Lidia Stanczyk, Tomasz Przygodzki, Cezary Watala
Article
Chemistry, Medicinal
Nina Wolska, Hassan Kassassir, Boguslawa Luzak, Cezary Watala, Marcin Rozalski
Article
Biochemistry & Molecular Biology
Adam Mieczkowski, Elzbieta Speina, Damian Trzybinski, Maria Winiewska-Szajewska, Patrycja Winska, Ewelina M. Borsuk, Malgorzata Podsiadla-Bialoskorska, Tomasz Przygodzki, Krzysztof Drabikowski, Lidia Stanczyk, Igor Zhukov, Cezary Watala, Krzysztof Wozniak
Summary: The study investigated the synthesis and cytotoxicity of novel analogues of the phosphodiesterase type 5 (PDE5) inhibitor-tadalafil, revealing that the parent compound exhibited stronger cytotoxic effects in most cases, except for specific cell lines. The newly obtained compounds showed no noticeable effect on PDE5 activity compared to the reference compound, suggesting potential for further research on selectivity and activity optimization for cytotoxic agents.
Article
Pharmacology & Pharmacy
Joanna Wzorek, Radoslaw Bednarek, Cezary Watala, Magdalena Boncler
Summary: The study found that PSB 0777 and cangrelor interact with human serum albumin with moderate affinity, but do not share the same binding site on the protein. This suggests that concurrent administration of these two antiplatelet compounds will not affect their antiplatelet activity due to competition-induced changes in drug binding to HSA.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dawid Polak, Marcin Talar, Nina Wolska, Dagmara W. Wojkowska, Kamil Karolczak, Karol Kramkowski, Tomasz A. Bonda, Cezary Watala, Tomasz Przygodzki
Summary: The A(2A) agonist HE-NECA in low doses in combination with inhibitors of P2Y(12) improved anti-thrombotic properties and was effective with chronic administration. Additionally, HE-NECA has hypotensive effects but does not significantly affect blood-brain barrier permeability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Ewelina Synowiec, Paulina Wigner, Natalia Cichon, Cezary Watala, Piotr Czarny, Joanna Saluk-Bijak, Elzbieta Miller, Tomasz Sliwinski, Ewa Zielinska-Nowak, Michal Bijak
Summary: The study identified specific genetic variants in SOD2, GPX4, NOS1, and NOS2 genes that are associated with an increased risk of stroke in the Polish population.
Article
Genetics & Heredity
Jussi J. Paterno, Ali Koskela, Juha M. T. Hyttinen, Elina Vattulainen, Ewelina Synowiec, Raimo Tuuminen, Cezary Watala, Janusz Blasiak, Kai Kaarniranta
Review
Biochemistry & Molecular Biology
Dawid Polak, Marcin Talar, Cezary Watala, Tomasz Przygodzki
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Anna Babinska, Cristina C. Clement, Yan Li, Joanna Wzorek, Tomasz Przygodzki, Marcin Talar, Marcin Braun, Maria Swiatkowska, Yigal H. Ehrlich, Elizabeth Kornecki, Cezary Watala, Moro O. Salifu