Article
Cell Biology
Noreen Mumtaz, Amel Dudakovic, Asha Nair, Marijke Koedam, Johannes P. T. M. van Leeuwen, Marion P. G. Koopmans, Barry Rockx, Andre J. J. van Wijnen, Bram C. J. van der Eerden
Summary: Arboviruses like Zika virus affect bone development by targeting osteoblasts and altering bone remodeling. In this study, the researchers investigated the effects of Zika virus infection on mesenchymal stromal cells (MSCs) at different stages of osteogenesis. They found that late-stage infected MSCs showed elevated osteogenic markers, calcium content, and altered gene expression compared to early-stage infected MSCs.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Cell & Tissue Engineering
Jeroen van de Peppel, Gerben J. Schaaf, Adriana Arruda Matos, Yuan Guo, Tanja Strini, Wenda Verschoor, Amel Dudakovic, Andre J. van Wijnen, Johannes P. T. M. van Leeuwen
Summary: This research suggests that the cell surface glycoprotein CD24 could serve as a selective biomarker for identifying subpopulations of BMSCs with varying osteogenic potential. CD24(pos) cells have increased expression of genes related to cell adhesion, motility, and extracellular matrix, while also showing reduced proliferation compared to CD24(neg) cells.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Engineering, Biomedical
Kevin Collon, Jennifer A. Bell, Stephanie W. Chang, Matthew C. Gallo, Osamu Sugiyama, Carolyn Marks, Jay R. Lieberman
Summary: This study compared different cell seeding techniques in transduced cell-based gene therapy. Static and negative pressure seeding techniques showed the highest seeding efficiency, while orbital shaking resulted in the greatest increase in BMP-2 production per cell. Low density cell suspensions were associated with the highest seeding efficiency, cell distribution uniformity, and the greatest increase in BMP-2 production after seeding.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Review
Cell Biology
Kevin Woods, Borhane Guezguez
Summary: MSCs are a heterogeneous cell population found in various tissues in the body, with important regulatory functions, especially in the bone marrow where they play a critical role in regulating HSCs. They contribute to maintaining bone integrity and hormonal nutrition supply, as well as regulating the homeostasis and differentiation of the HSC niche through differentiation into different cell types. Research on MSCs helps understand their role in regulating cell interactions and homeostasis in the body.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Kevin Woods, Borhane Guezguez
Summary: Mesenchymal stromal cells (MSCs) are a diverse cell population known for their nutrient-producing and immunomodulatory functions, crucial in regulating hematopoietic stem cells (HSC) in the bone marrow for blood production and immunity. MSCs contribute to bone integrity and hormone supply by differentiating into osteoblasts and adipocytes, playing a key role in the complex interactions of the HSC niche.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell & Tissue Engineering
Mesude Bicer, Graeme S. Cottrell, Darius Widera
Summary: As populations age, osteoporosis and osteoporosis-related fractures are becoming increasingly prevalent degenerative bone diseases. Three-dimensional cell culture technology may serve as an alternative therapeutic approach to tackle osteoporosis, allowing for more physiological expansion and differentiation of stem cells.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Xiaoru Xu, Shuang Song, Xiangdong Liu, Yuchao Zhou, Shaojie Shi, Guoqiang Zhao, Xingxing Wang, Xutao Chen, Wenshuang Zhao, Sijia Zhang, Wei Ma, Yingliang Song
Summary: Cell sheet technology utilizing adipose-derived stromal cells (ASCs) shows promise in promoting angiogenesis and may have valuable clinical applications.
STEM CELLS INTERNATIONAL
(2022)
Article
Cell Biology
Sarah Hochmann, Kristy Ou, Rodolphe Poupardin, Michaela Mittermeir, Martin Textor, Salaheddine Ali, Martin Wolf, Agnes Ellinghaus, Dorit Jacobi, Juri A. J. Elmiger, Samantha Donsante, Mara Riminucci, Richard Schaefer, Uwe Kornak, Oliver Klein, Katharina Schallmoser, Katharina Schmidt-Bleek, Georg N. Duda, Julia K. Polansky, Sven Geissler, Dirk Strunk
Summary: Multipotent stromal cells are attractive sources for cell therapy and tissue engineering, but their application is limited due to functional diversity. In this study, we compared the regenerative potential of different stromal cell types in vitro and in vivo and found that only bone marrow stromal cells (BMSCs) could fully regenerate critical-size femoral defects. This ability was attributed to cell type-specific epigenetic landscapes controlling transcriptional stromal differentiation networks. BMSCs initiated defect healing and eventually contributed to tissue remodeling.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Dan Xing, Kai Wang, Jun Wu, Yu Zhao, Wei Liu, Jiao Jiao Li, Tingting Gao, Deng Yan, Liu Wang, Jie Hao, Jianhao Lin
Summary: The study demonstrates that multiple injections of ES-MSCs can improve osteoarthritis in a rat model. The repeated doses group showed more proteoglycan and less cartilage loss, along with lower Mankin scores, indicating the potential of ES-MSCs as a regenerative therapy for OA.
Review
Biochemistry & Molecular Biology
Yujie Song, Christian Jorgensen
Summary: This review highlights the potential of mesenchymal stromal cells (MSCs) in treating osteoarthritis (OA), focusing on their chondrogenic and immunomodulatory potential. The review provides an overview of the effects of MSCs in preclinical and clinical studies of OA treatment, comparing different MSC sources. Furthermore, the review summarizes the current evidence for the assessment of cartilage in OA using magnetic resonance imaging (MRI) and second-look arthroscopy after MSC therapy.
Review
Biotechnology & Applied Microbiology
Yun-Feng Zhou, Di Zhang, Wan-Ting Yan, Kai Lian, Zheng-Zheng Zhang
Summary: This article reviews the therapeutic potential of multipotent stromal cells (MSCs) in repairing meniscal injuries and discusses the avenues for utilizing these cells. The current progress on biomaterial implants is also reviewed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Raphael Gorodetsky, Wilhelm K. Aicher
Summary: MSCs from different sources can release pro-regenerative and anti-inflammatory factors, with hPSCs from the fresh human placenta being a preferred source for abundant cell harvesting. The f-hPSCs from neonate tissues of the placenta can undergo extended expansion with low risk of senescence, making them competent cells for therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Medicine, General & Internal
Hideaki Kagami, Minoru Inoue, Hideki Agata, Izumi Asahina, Tokiko Nagamura-Inoue, Masataka Taguri, Arinobu Tojo
Summary: By optimizing the cell-processing protocol, stable bone regeneration can be achieved with comparable quality and less fluctuation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Siddharth Shanbhag, Neha Rana, Salwa Suliman, Shaza Bushra Idris, Kamal Mustafa, Andreas Stavropoulos
Summary: This study investigated the influence of bi-calcium phosphates (BCPs) on mesenchymal stromal cells (MSCs) and MSC-macrophage interactions in an inflammatory microenvironment. The results showed that BCPs significantly regulated MSC gene expressions toward a pro-healing phenotype under both inflammatory and non-inflammatory conditions, with little effect on MSC secretory profiles. In the presence of macrophages, BCPs positively regulated both gene expression and cytokine secretion of MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
