Article
Reproductive Biology
Yijing He, Lei Peng, Jia Li, Qiao Li, Yajie Chu, Qixin Lin, Rong Rui, Shiqiang Ju
Summary: TPX2 plays an important role in meiotic spindle formation in porcine oocytes and interacts with microtubule-associated proteins AURKA and TACC3.
Article
Cell Biology
Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle
Summary: The research identified TACC3 and clathrin as core members, and chTOG and GTSE1 as ancillary members of the complex. Additionally, PIK3C2A was shown not to be a part of the complex. Targeting the TACC3-clathrin interface or their microtubule-binding sites are the two most likely strategies to disrupt spindle stability mediated by this multiprotein complex.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Wonkyung Oh, Ting Ting Wu, Seo-Yeon Jeong, Ho Jin You, Jung-Hee Lee
Summary: CtIP plays a critical role in regulating mitosis by interacting with TPX2 to control spindle dynamics and regulate the concentration of Aurora A and microtubule intensity at the spindle poles. Depletion of CtIP may lead to improper execution of mitosis and result in chromosomal instability.
Article
Biochemistry & Molecular Biology
Jian Liu, Hongsheng Xue, Chunxiao Li, Xiangyu Chen, Jiannan Yao, Dongkui Xu, Haili Qian
Summary: We identified the subcellular distribution of MTA1 in mitotic cancer cells and its role in inhibiting SAC activation. MTA1 is localized in the spindle apparatus throughout mitosis and interacts with TPR. The MTA1-TPR complex represents a distinct SAC-associated complex separate from the TPR-MAD1/MAD2 complex.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jodi D. Weiss, Shelby L. McVey, Sarah E. Stinebaugh, Caroline F. Sullivan, R. Kelly Dawe, Natalie J. Nannas
Summary: The assembly and disassembly of spindles in maize meiosis exhibit variability but follow a predictable series of events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Si-Cheng Zhao, Feng-Xin Qiao, Ming-Xin Sun, Yue-Cen Liu, Hai-Long Wang, Zhi-Ran Xu, Yu Liu
Summary: This study found that cobalt chloride treatment affects ovary function, leading to decreased oocyte quality, impaired follicular growth, disrupted spindle assembly, and mitochondrial dysfunction.
Article
Plant Sciences
Chong Wang, Shuying Qu, Jie Zhang, Ming Fu, Xiaofei Chen, Wanqi Liang
Summary: Meiotic recombination begins with the formation of double-strand breaks in DNA, which is catalyzed by SPO11 and accessory proteins like MEI4/PRD2. In rice, the MEI4/PRD2 homolog OsPRD2 plays a crucial role in meiosis, primarily involved in the generation of chromosomes and assembly of the spindle. Although OsPRD2 does not participate in spindle assembly in rice, it plays a conserved role in the formation of double-strand breaks in DNA.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cell Biology
Lin-Lin Hu, Meng-Hao Pan, Feng-Lian Yang, Zi-Ao Zong, Feng Tang, Zhen-Nan Pan, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Shao-Chen Sun
Summary: Research demonstrates that FASCIN is crucial for mouse oocyte maturation, affecting spindle migration and polar body extrusion. It may be regulated by PKC and plays a significant role in meiosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lei Peng, Yijing He, Weihan Wang, Yajie Chu, Qixin Lin, Rong Rui, Qiao Li, Shiqiang Ju
Summary: In this study, the importance of PAK1 in regulating spindle assembly and cell cycle progression during the first meiotic division of porcine oocytes was highlighted. The activated PAK1 showed dynamic expression and its subcellular localization was closely associated with spindle dynamics. Inhibition of PAK1 activity by IPA-3 led to impaired extrusion of PB1 and failure of bipolar spindle formation, which could be restored by reducing disulfide bonds between PAK1 and IPA-3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Da-Jie Deng, Xi Wang, Kai-Ye Yue, Yamei Wang, Quan-Wen Jin
Summary: This study investigated the role of fission yeast PP2A in SAC silencing. The results showed that the absence of two B56 regulatory subunits of PP2A only slightly delayed SAC inactivation. Overexpression of individual PP2A subunits could only slightly suppress the SAC silencing defects in PP1 deletion cells. Therefore, fission yeast PP2A is not a key regulator actively involved in SAC inactivation.
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Zhen-Nan Pan, Kun-Huan Zhang, Yi-Ming Ji, Jing-Cai Liu, Shao-Chen Sun
Summary: This study demonstrates that Mcrs1 plays important roles in regulating the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes during meiosis I. Depletion of Mcrs1 affects histone modifications, CDK1 activity, and cyclin B1 accumulation, leading to delayed G2/M transition and abnormal spindle assembly.
Article
Multidisciplinary Sciences
Ana C. Henriques, Patricia M. A. Silva, Bruno Sarmento, Hassan Bousbaa
Summary: Antimitotic drugs can induce cell death by activating the spindle assembly checkpoint, but cancer cells treated with these drugs can escape death through mitotic slippage. By inhibiting SAC silencing and delaying mitotic slippage, the effectiveness of antimitotic drugs can be improved, leading to enhanced cell death. Inhibition of SAC silencing also sensitizes cancer cells to apoptosis-inducing small molecules, suggesting a potential therapeutic strategy for antimitotic therapies.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Stanislau Yatskevich, Jessie S. Kroonen, Claudio Alfieri, Thomas Tischer, Anna C. Howes, Linda Clijsters, Jing Yang, Ziguo Zhang, Kaige Yan, Alfred C. O. Vertegaal, David Barford
Summary: SUMOylation of the APC/C rearranges the structure of the WHB domain of the cullin subunit, leading to reduced affinity of APC/C-Cdc20 for the mitotic checkpoint complex and allowing for more efficient ubiquitination of substrates in the presence of the MCC, contributing to timely anaphase onset.
Article
Reproductive Biology
Cecilia S. Blengini, Karen Schindler
Summary: This article discusses the basic mechanisms of spindle formation during meiotic maturation, focusing on studies in mice and humans. It also explores various factors that may influence spindle formation and stability, as well as the potential impact of assisted reproductive technologies on spindles and subsequent embryo development.
BIOLOGY OF REPRODUCTION
(2022)
Article
Chemistry, Medicinal
Wenxuan Zhao, Xuyang Sun, Lei Shi, Shi-zhong Cai, Zhou-rui Ma
Summary: Fifteen compounds were designed and synthesized in this study, with the most potent compound 7g showing about 10-folds more potent antiproliferative activities than KHS101 in various cancer cell lines and being confirmed as a biologically relevant target of TACC3. Compound 7g also exhibited significant effects on cell cycle arrest, cell apoptosis, MMP depolarization, and ROS generation.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
J. Aretz, Y. Kondoh, K. Honda, U. R. Anumala, M. Nazare, N. Watanabe, H. Osada, C. Rademacher
CHEMICAL COMMUNICATIONS
(2016)
Article
Chemistry, Multidisciplinary
Taro Noguchi, Shinya Oishi, Kaori Honda, Yasumitsu Kondoh, Tamio Saito, Hiroaki Ohno, Hiroyuki Osada, Nobutaka Fujii
CHEMICAL COMMUNICATIONS
(2016)
Meeting Abstract
Oncology
T. Kawamura, M. Kawatani, M. Muroi, Y. Kondoh, Y. Futamura, H. Aono, M. Tanaka, K. Honda, H. Osada
EUROPEAN JOURNAL OF CANCER
(2016)
Article
Biotechnology & Applied Microbiology
Shota Uesugi, Makoto Muroi, Yasumitsu Kondoh, Yoshihito Shiono, Hiroyuki Osada, Ken-ichi Kimura
JOURNAL OF ANTIBIOTICS
(2017)
Article
Cell Biology
Masataka Kunii, Mica Ohara-Imaizumi, Noriko Takahashi, Masaki Kobayashi, Ryosuke Kawakami, Yasumitsu Kondoh, Takeshi Shimizu, Siro Simizu, Bangzhong Lin, Kazuto Nunomura, Kyota Aoyagi, Mitsuyo Ohno, Masaki Ohmuraya, Takashi Sato, Shin-ichiro Yoshimura, Ken Sato, Reiko Harada, Yoon-Jeong Kim, Hiroyuki Osada, Tomomi Nemoto, Haruo Kasai, Tadahiro Kitamura, Shinya Nagamatsu, Akihiro Harada
JOURNAL OF CELL BIOLOGY
(2016)
Article
Immunology
Michihiro Hashimoto, Farzana Bhuyan, Masateru Hiyoshi, Osamu Noyori, Hesham Nasser, Mitsue Miyazaki, Tamio Saito, Yasumitsu Kondoh, Hiroyuki Osada, Shunsuke Kimura, Koji Hase, Hiroshi Ohno, Shinya Suzu
JOURNAL OF IMMUNOLOGY
(2016)
Article
Plant Sciences
Wen-Dee Ong, Emiko Okubo-Kurihara, Yukio Kurihara, Setsuko Shimada, Yuko Makita, Mika Kawashima, Kaori Honda, Yasumitsu Kondoh, Nobumoto Watanabe, Hiroyuki Osada, Sean R. Cutler, Kumar Sudesh, Minami Matsui
PLANT AND CELL PHYSIOLOGY
(2017)
Article
Multidisciplinary Sciences
Tatsuro Kawamura, Makoto Kawatani, Makoto Muroi, Yasumitsu Kondoh, Yushi Futamura, Harumi Aono, Miho Tanaka, Kaori Honda, Hiroyuki Osada
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Yasumitsu Kondoh, Kaori Honda, Sayoko Hiranuma, Teruo Hayashi, Takeshi Shimizu, Nobumoto Watanabe, Hiroyuki Osada
SCIENTIFIC REPORTS
(2016)
Article
Oncology
Ken-ichi Takayama, Teruki Honma, Takashi Suzuki, Yasumitsu Kondoh, Hiroyuki Osada, Yutaka Suzuki, Minoru Yoshida, Satoshi Inoue
Summary: This study identifies small molecules that target PSF-RNA interactions and suppress hormone therapy-refractory cancer growth, offering potential in reversing epigenetically repressed gene regulation and inhibiting tumor proliferation.
Article
Biochemistry & Molecular Biology
Hiromasa Yoshioka, Tatsuro Kawamura, Makoto Muroi, Yasumitsu Kondoh, Kaori Honda, Makoto Kawatani, Harumi Aono, Herbert Waldmann, Nobumoto Watanabe, Hiroyuki Osada
Summary: The compound NPD4928 was identified to enhance the sensitivity of various cancer cells to GPX4 inhibitors, potentially having therapeutic potential via the induction of ferroptosis.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biology
Takeharu Sakamoto, Yuya Fukui, Yasumitsu Kondoh, Kaori Honda, Takeshi Shimizu, Toshiro Hara, Tetsuro Hayashi, Yurika Saitoh, Yoshinori Murakami, Jun-ichiro Inoue, Shuichi Kaneko, Hiroyuki Osada, Motoharu Seiki
Summary: The study identifies naphthofluorescein as a Mint3 inhibitor that disrupts the Mint3-FIH-1 interaction and attenuates HIF-1 activity. In vivo experiments in mice show a reduction in tumor growth with attenuated inflammatory cytokine production and endotoxic shock, providing a potential option for targeted therapies for cancer and inflammatory diseases that avoid severe adverse effects.
COMMUNICATIONS BIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Takayuki Motoyama, Yasumitsu Kondoh, Takeshi Shimizu, Teruo Hayashi, Kaori Honda, Motoko Uchida, Hiroyuki Osada
Summary: This study aimed to identify inhibitors for SDH1-V75M by screening the RIKEN Natural Products Depository using chemical array technology. The compound NPD13731 strongly inhibited the activity of both wild-type and mutant SDH1. A more potent inhibitor, compound 16 (melabiostin), was created based on structure-activity relationship data and showed control over rice blast disease caused by MBI-D-resistant P. oryzae infections.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Takeo Nakaya, Kenichi Aizawa, Yuki Taguchi, Kentaro Tsuji, Sachi Sekine, Kazuhiro Murakami, Masaji Kasai, Hirofumi Nakano, Yasumitsu Kondoh, Shingo Dan, Atsushi Yoshimori, Hiroyuki Kouji, Dai Takehara, Toru Suzuki, Hiroyuki Osada, Masayuki Murata, Akira Tanaka, Ryozo Nagai
Summary: This study focused on the hydrophobic alpha-helix structure of the disordered protein KLF5, identifying a potential interface for protein-protein interaction. Low-molecular-weight compounds were synthesized to selectively suppress the proliferation and survival of cancer cells by targeting KLF5 and the Wnt signaling pathway. These compounds demonstrated promising results in vivo without side effects.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemical Research Methods
Taro Noguchi, Hiroyuki Ishiba, Kaori Honda, Yasumitsu Kondoh, Hiroyuki Osada, Hiroaki Ohno, Nobutaka Fujii, Shinya Oishi
BIOCONJUGATE CHEMISTRY
(2017)