Article
Dentistry, Oral Surgery & Medicine
B. Sengueven Tokozlu, D. Sapkota, E. M. Vallenari, O. Schreurs, T. M. Soland
Summary: Oral squamous cell carcinoma of the tongue (OTSCC) is a common and unfavorable malignancy. Overexpression of cortactin is associated with invasion and metastasis of tumor cells. This study investigates the expression pattern and prognostic significance of cortactin in HPV-negative OTSCC, finding that high overall cortactin expression is associated with larger tumor size, distant metastasis, and reduced 5-year survival. Silencing cortactin suppresses the proliferative and invasive abilities of OTSCC cells.
EUROPEAN JOURNAL OF ORAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Serena Calamaio, Marialaura Serzanti, Jennifer Boniotti, Annamaria Fra, Emirena Garrafa, Manuela Cominelli, Rosanna Verardi, Pietro Luigi Poliani, Silvia Dotti, Riccardo Villa, Giovanna Mazzoleni, Patrizia Dell'Era, Nathalie Steimberg
Summary: The process of developing a new drug is time-consuming and expensive, and hepatotoxicity is a major concern. Animal models have limitations due to interspecies differences, but human induced pluripotent stem cells (hiPSCs) offer new possibilities for drug testing. We miniaturized the process of generating hepatic organoids using hiPSC-derived mesenchymal stem cells, hepatocytes, and endothelial cells. These organoids were cultivated in vitro using static and dynamic cultures, and could be maintained for up to 1 week. This hiPSC-derived liver organoid model could potentially replace animal testing in pharmacological and toxicological studies.
Article
Cell Biology
Liang Luo, Wei Zhang, Jing Wang, Ming Zhao, Kuo Shen, Yanhui Jia, Yan Li, Jian Zhang, Weixia Cai, Dan Xiao, Xiaozhi Bai, Kaituo Liu, Kejia Wang, Yue Zhang, Huayu Zhu, Qin Zhou, Dahai Hu
Summary: A new 3D culture method for adipose-derived mesenchymal stem cells based on a self-feeder layer has been developed, showing better proliferation ability and cell viability compared to traditional ultra-low attachment (ULA) methods. The unique properties of the SLF-3D spheroid are likely due to changes in the expression of ECM-related genes, such as COL3A1, MMP3, HAS1, and FN1, making it a promising approach for future clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Maryam Parsian, Pelin Mutlu, Ender Yildirim, Can Ildiz, Can Ozen, Ufuk Gunduz
Summary: The study introduces a new tissue culture approach that combines a microfluidic chip with microdissected breast cancer tumors, maintaining the viability of tissue samples and enabling stable culturing of tumor tissues compared to conventional methods. This new culturing technique may have the potential to develop new treatment strategies more effectively.
Article
Endocrinology & Metabolism
Xiumei Feng, Qingchun Zhang, Qianjin Xu, Meiqin Shao
Summary: This study investigated the effects of miR-202 and GLI2 on cell metastasis and epithelial-mesenchymal transition (EMT) in cervical cancer (CC). The results showed that overexpression of miR-202 inhibited CC cell metastasis and EMT by regulating GLI2 mRNA expression. This finding provides new insights for the clinical treatment of CC.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Biochemistry & Molecular Biology
Garrett Winkelmaier, Kosar Jabbari, Lung-Chang Chien, Peter Grabham, Bahram Parvin, Janice Pluth
Summary: During space travel, astronauts face the challenge of adapting to microgravity and stressful living conditions. The impact of microgravity on organ development is not well understood, and this study used mouse mammary epithelial cells to investigate its effects. The results suggest that microgravity can cause abnormal changes in mammary epithelial cells and increase the risk of cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Cuimin Chen, Junliang Lu, Huanwen Wu
Summary: In this study, a novel PTCH1::GLI2 gene fusion was identified in a patient with gastroblastoma, expanding the molecular spectrum of this tumor. This finding is important for understanding the pathogenesis of gastroblastoma.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Isabella B. Metelmann, Sebastian Kraemer, Matthias Steinert, Stefan Langer, Peggy Stock, Olga Kurow
Summary: This study developed a 3D organotypic model of the pleural mesothelium and demonstrated its similarity to human pleura tissue in terms of morphology and microenvironment. The permeability test of the model indicated its ability to mimic physiological conditions in vivo.
Article
Dermatology
Melanie Schwab, Sabrina Lohr, Jakob Schneider, Michaela Kaiser, Damir Krunic, Doris Helbig, Cyrill Geraud, Peter Angel
Summary: The study demonstrates that loss of PDPN in murine cSCC cells leads to decreased migration and invasion in vitro, highlighting the crucial role of PDPN in cSCC progression. Experiments with Pdpn mutants further reveal the importance of both intra- and extracellular domains in cancer cell invasion. Orthotopic injection of labeled cells into nude mice confirms the impact of PDPN on tumor infiltration into the stroma.
EXPERIMENTAL DERMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Lin He, Chuxia Deng
Summary: Organotypic tissue slice culture has numerous advantages in anticancer drug development, including the preservation of cell repertoire, quick assessment of therapeutic efficacy, and the ability to select personalized treatment regimens. It is expected to become a potent platform for individualized drug screening.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Oncology
Jonathan Robert Weitz, Herve Tiriac, Tatiana Hurtado de Mendoza, Alexis Wascher, Andrew M. Lowy
Summary: Pancreatic ductal adenocarcinoma (PDAC) has the highest mortality rate among major cancers, and current therapies have had limited success. Understanding the complex tumor microenvironment (TME) of PDAC, including fibrotic matrix and immunosuppressive features, is crucial for developing effective treatments. Organotypic tumor slices from patient tumors provide a valuable model system to study TME interactions and potential therapeutic approaches for personalized cancer therapy.
Article
Biochemistry & Molecular Biology
Yue Li, Shan Zeng, Fengjie Zhou, Huiqun Jie, Dongzhen Yu, Shule Hou, Penghui Chen, Dekun Gao, Yupeng Liu, Jun Yang, Jingchun He
Summary: Cisplatin is an effective drug for solid tumors, but it often causes ototoxicity. This study found that overexpression of XIAP can protect cochlear hair cells from cisplatin-induced injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Clinical Neurology
Gareth Morris, Massimo Avoli, Christophe Bernard, Kate Connor, Marco de Curtis, Chris G. Dulla, John G. R. Jefferys, Caterina Psarropoulou, Kevin J. Staley, Mark O. Cunningham
Summary: In vitro preparations offer valuable tools for studying and modeling seizures and epilepsy, as well as screening anti-seizure compounds. However, these models are simplifications of reality and have limitations. Therefore, a combination of in vitro models, in vivo studies, chronic epilepsy animal models, and human cell/tissue-derived preparations is recommended to maximize their potential value.
Article
Biochemistry & Molecular Biology
Danyang Wang, Simone Hildorf, Elissavet Ntemou, Linn Salto Mamsen, Lihua Dong, Susanne Elisabeth Pors, Jens Fedder, Erik Clasen-Linde, Dina Cortes, Jorgen Thorup, Claus Yding Andersen
Summary: This study showed that organotypic culture of testicular tissue from infants with cryptorchidism could support the development of spermatogonia and somatic cells, with the addition of retinoic acid (RA) inducing spermatocytes and enhancing the maturation of Sertoli cells. The absence of RA in the culture medium led to an increase in peritubular myoid cells. This research provides insights for future studies on in vitro spermatogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dermatology
Rishika Agarwal, Tanja Dittmar, Hans-Dietmar Beer, Michael Kunz, Simon Mueller, Elisabeth A. A. Kappos, Emmanuel Contassot, Alexander A. Navarini
Summary: This study presents a method for generating human epidermis organotypic cultures (HEOCs) from primary keratinocytes and an immortalized keratinocyte cell line (KerTr). The HEOCs were characterized and shown to express markers associated with proliferation and differentiation. The ability to generate HEOCs reproducibly on a large scale makes them a valuable model for drug screening and studying epidermal pathologies.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Neurosciences
Kentaro Ono, Yi Ye, Chi T. Viet, Dongmin Dang, Brian L. Schmidt
JOURNAL OF NEUROPHYSIOLOGY
(2015)
Article
Health Care Sciences & Services
Fay Wright, Gail D'Eramo Melkus, Marilyn Hammer, Brian L. Schmidt, M. Tish Knobf, Steven M. Paul, Frances Cartwright, Judy Mastick, Bruce A. Cooper, Lee-May Chen, Michelle Melisko, Jon D. Levine, Kord Kober, Bradley E. Aouizerat, Christine Miaskowski
JOURNAL OF PAIN AND SYMPTOM MANAGEMENT
(2015)
Article
Health Care Sciences & Services
Dale J. Langford, Brian Schmidt, Jon D. Levine, Gary Abrams, Charles Elboim, Laura Esserman, Deborah Hamolsky, Judy Mastick, Steven M. Paul, Bruce Cooper, Kord Kober, Marylin Dodd, Laura Dunn, Bradley Aouizerat, Christine Miaskowski
JOURNAL OF PAIN AND SYMPTOM MANAGEMENT
(2015)
Article
Health Care Sciences & Services
Fay Wright, Gail D'Eramo Melkus, Marilyn Hammer, Brian L. Schmidt, Tish Knobf, Steven M. Paul, Frances Cartwright, Judy Mastick, Bruce A. Cooper, Lee-May Chen, Michelle Melisko, Jon D. Levine, Kord Kober, Bradley E. Aouizerat, Christine Miaskowski
JOURNAL OF PAIN AND SYMPTOM MANAGEMENT
(2015)
Article
Oncology
Kenichiro Uchida, Ratna Veeramachaneni, Bing Huey, Aditi Bhattacharya, Brian L. Schmidt, Donna G. Albertson
Article
Oncology
Christine Miaskowski, Bruce A. Cooper, Michelle Melisko, Lee-May Chen, Judy Mastick, Claudia West, Steven M. Paul, Laura B. Dunn, Brian L. Schmidt, Marilyn Hammer, Frances Cartwright, Fay Wright, Dale J. Langford, Kathryn Lee, Bradley E. Aouizerat
Review
Dentistry, Oral Surgery & Medicine
C. T. Viet, P. M. Corby, A. Akinwande, B. L. Schmidt
JOURNAL OF DENTAL RESEARCH
(2014)
Article
Genetics & Heredity
Dale J. Langford, Claudia West, Charles Elboim, Bruce A. Cooper, Gary Abrams, Steven M. Paul, Brian L. Schmidt, Jon D. Levine, John D. Merriman, Anand Dhruva, John Neuhaus, Heather Leutwyler, Christina Baggott, Carmen Ward Sullivan, Bradley E. Aouizerat, Christine Miaskowski
JOURNAL OF NEUROGENETICS
(2014)
Article
Clinical Neurology
Dale J. Langford, Steven M. Paul, Claudia West, Jon D. Levine, Deborah Hamolsky, Charles Elboim, Brian L. Schmidt, Bruce A. Cooper, Gary Abrams, Bradley E. Aouizerat, Christine Miaskowski
Article
Clinical Neurology
Kimberly Stephens, Bruce A. Cooper, Claudia West, Steven M. Paul, Christina R. Baggott, John D. Merriman, Anand Dhruva, Kord M. Kober, Dale J. Langford, Heather Leutwyler, Judith A. Luce, Brian L. Schmidt, Gary M. Abrams, Charles Elboim, Deborah Hamolsky, Jon D. Levine, Christine Miaskowski, Bradley E. Aouizerat
Article
Medicine, Research & Experimental
Geraldine Leung, Christina Baggott, Claudia West, Charles Elboim, Steven M. Paul, Bruce A. Cooper, Gary Abrams, Anand Dhruva, Brian L. Schmidt, Kord Kober, John D. Merriman, Heather Leutwyler, John Neuhaus, Dale Langford, Betty J. Smoot, Bradley E. Aouizerat, Christine Miaskowski
LYMPHATIC RESEARCH AND BIOLOGY
(2014)
Article
Medicine, Research & Experimental
Betty Smoot, Steven M. Paul, Bradley E. Aouizerat, Charles Elboim, Jon D. Levine, Gary Abrams, Deborah Hamolsky, John Neuhaus, Brian Schmidt, Claudia West, Kimberly Topp, Christine Miaskowski
LYMPHATIC RESEARCH AND BIOLOGY
(2014)
Article
Clinical Neurology
Brian L. Schmidt
Article
Multidisciplinary Sciences
Brian L. Schmidt, Justin Kuczynski, Aditi Bhattacharya, Bing Huey, Patricia M. Corby, Erica L. S. Queiroz, Kira Nightingale, A. Ross Kerr, Mark D. DeLacure, Ratna Veeramachaneni, Adam B. Olshen, Donna G. Albertson
Article
Multidisciplinary Sciences
Chi T. Viet, Dongmin Dang, Stacy Achdjian, Yi Ye, Samuel G. Katz, Brian L. Schmidt