Weak base pairing in both seed and 3′ regions reduces RNAi off-targets and enhances si/shRNA designs

Modifications in Small Interfering RNA That Separate Immunostimulation from RNA Interference

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Mapping the Human miRNA Interactome by CLASH Reveals Frequent Noncanonical Binding

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A Systematic Mammalian Genetic Interaction Map Reveals Pathways Underlying Ricin Susceptibility

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The Loop Position of shRNAs and Pre-miRNAs Is Critical for the Accuracy of Dicer Processing In Vivo

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Argonaute Divides Its RNA Guide into Domains with Distinct Functions and RNA-Binding Properties

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Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease

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Argonaute proteins are key determinants of RNAi efficacy, toxicity, and persistence in the adult mouse liver

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Mammalian microRNAs predominantly act to decrease target mRNA levels

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siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect

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Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application

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Biological basis for restriction of microRNA targets to the 3′ untranslated region in mammalian mRNAs

(2009) Shuo Gu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Thermodynamic stability and Watson–Crick base pairing in the seed duplex are major determinants of the efficiency of the siRNA-based off-target effect

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Experimental validation of the importance of seed complement frequency to siRNA specificity

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