Article
Engineering, Environmental
Naihui Li, Ruixue Chang, Shuo Chen, Jilin Lei, Yanli Liu, Wenjing Cui, Qing Chen, Fengzhi Wu
Summary: This study evaluated the suppressive effects of pig manure biogas slurry and vinasse biogas slurry on pathogen mycelial growth and cucumber fusarium wilt. The microbial communities of the slurries were analyzed, and key taxa related to disease resistance were identified. The findings showed that the slurries had similar suppression on the pathogen and effectively controlled cucumber fusarium wilt.
Article
Agriculture, Dairy & Animal Science
Arkadiusz Pietruszka, Marta Maslanko, Daria Ciecholewska-Jusko
Summary: This study investigates the impact of the methane fermentation process in two agricultural biogas plants on the sanitization efficiency of pig slurry. The plants differed in the type of substrate used, with one using pig slurry from a maternal farm and the other utilizing pig slurry from a fattening farm. The results show that using pig slurry from a fattening farm as a substrate leads to significantly higher sanitization effectiveness, which provides clear recommendations for the location of biogas plants.
Article
Engineering, Environmental
Hambaliou Balde, Claudia Wagner-Riddle, Douglas MacDonald, Andrew VanderZaag
Summary: This study quantified the fugitive methane losses from two biogas facilities over one year and found that digestate storage was the main source of losses. Leakage and flaring also contributed to the overall methane losses. Facility B had higher losses, but the leakage emissions were reduced after dome membrane repair. Minimizing losses from digestate storage and avoiding leakage during abnormal operation are crucial for biogas to have a positive impact on greenhouse gas emissions.
Article
Energy & Fuels
Grzegorz Ginda, Marta Szyba
Summary: Agricultural biogas plants serve as both a renewable energy source and a means of biodegradable waste disposal in the agriculture and food industry. However, the development of such plants in Poland has been hindered by several factors including technology, substrates, energy policy, profitability, population density, and spatial arrangement. Through a pairwise comparison analysis using the DEMATEL method, it was determined that population density and the lack of local spatial development plans are the most influential factors. Improvements can be made by implementing spatial plans throughout each commune and locating biogas plants near livestock farms and agri-food processing plants.
Article
Environmental Sciences
Yong Wei Tiong, Pooja Sharma, Hailin Tian, To-Hung Tsui, Heng Thong Lam, Yen Wah Tong
Summary: The aim of this study was to evaluate the feasibility of using a pilot-scale anaerobic digester to treat food waste generated from a hawker centre in an urban area of Singapore. The results showed that the decentralized system had an average specific methane yield of 0.55 +/- 0.04 m3CH4/kgVS, with methane concentrations of 56.6 +/- 2.3%. The energy assessment revealed an average consumption rate of 2.05 +/- 0.57 m3/kW h for power generation. Additionally, the study identified the microbial communities present in the digester during the startup phase, providing new insights into decentralized anaerobic digestion of food waste.
Article
Energy & Fuels
Magdalena Tyminska, Zbigniew Skibko, Andrzej Borusiewicz
Summary: Agricultural biogas plants, although possessing numerous advantages, are less popular compared to photovoltaic or wind power plants. The lack of support for constructing new agricultural biogas plants can be attributed to concerns regarding their potential interference with the electricity grid connected to nearby consumers. However, research demonstrates that these plants do not significantly impact the power quality of the grid. Detailed analysis revealed that the operation of the digester mixer in the fermenter caused the most notable changes in voltage parameters in the studied farm specializing in barnless cattle rearing. Additionally, the study identified a previously unnoticed problem with the impact of the digester mixer on the energy parameters produced in biogas plants, which manufacturers have yet to address.
Article
Environmental Sciences
He Sun, Anna Schnurer, Bettina Mueller, Bettina Moessnang, Michael Lebuhn, Oliwia Makarewicz
Summary: Antimicrobial resistance is a global concern, and anaerobic digestion processes can potentially transmit antimicrobial resistant contaminants, including bacteria and genes, through the use of plant-based substrates. This study examined three biogas plants to assess the presence of antimicrobial resistance in plant-based substrates and found that while the bacterial community mainly consisted of Gram-positive bacteria, antimicrobial resistance genes and plasmids were present, indicating the existence of Gram-negative pathogenic bacteria. However, digestates had lower levels of antimicrobial resistance than the substrates themselves. Thus, plant-based substrates should be considered in agricultural biogas processing to address the issue of antimicrobial resistance.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Plant Sciences
Mohammad Bahram, Kati Kungas, Mad Pent, Sergei Polme, Daniyal Gohar, Kadri Poldmaa
Summary: Bacterial and fungal endophytes play a significant role in the performance and health of host plants. This study reveals that the structure of bacterial and fungal communities in trees is more influenced by the vertical location of tree compartments rather than the locality, species, and individuals of host trees. The microbial communities show stronger host specificity aboveground compared to belowground compartments. Bacterial communities are more influenced by environmental conditions, while fungal communities are more influenced by host identity.
PHYTOBIOMES JOURNAL
(2022)
Article
Microbiology
Xiaofeng Chen, Kai Tang, Mu Zhang, Shujing Liu, Mingming Chen, Peiwen Zhan, Wei Fan, Chen-Tung Arthur Chen, Yao Zhang
Summary: By studying the genome and protein expression of microorganisms in the shallow-sea hydrothermal vent fluids off Kueishantao Island, researchers have revealed the genetic potentials and protein expressions of microbial communities in this system, as well as their connections with deep-sea and terrestrial geothermal systems.
Article
Energy & Fuels
Ghaith Hasan, Jana Mazancova, Jan Banout, Raed Jafar, Hynek Roubik
Summary: This study examines the economic feasibility of using organic waste for biogas production in Syria and compares the profitability of units depending on plant residues and animal waste. The research findings indicate that biogas units utilizing crop residues are more profitable and should be considered in renewable energy programs, especially considering the government's interest in renewable energies and the abundance of crop residues in the Syrian environment.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Review
Energy & Fuels
Nwabunwanne Lilian Nwokolo, Matthew Chekwube Enebe
Summary: Biogas production from organic waste is a sustainable means of meeting household energy needs, and understanding and controlling biotic and abiotic factors can enhance microbial performance and promote biogas production.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Energy & Fuels
Kamila Klimek, Magdalena Kaplan, Serhiy Syrotyuk, Nikolay Bakach, Nikolay Kapustin, Ryszard Konieczny, Jakub Dobrzynski, Kinga Borek, Dorota Anders, Barbara Dybek, Agnieszka Karwacka, Grzegorz Walowski
Summary: The main idea of circular economy is to decouple economic growth from resource consumption and environmental impacts, with a focus on minimizing waste generation and promoting innovation throughout the value chain. Agricultural biogas plants play a crucial role in managing waste biomass and converting it into energy and fertilizer, contributing to the effectiveness of circular economy practices. By developing a financial model and considering investment aspects, this study provides guidance on investing in the renewable energy market, particularly in the context of agricultural biogas projects.
Article
Agronomy
Yanqing Guo, Hui Luo, Li Wang, Mingying Xu, Yongshan Wan, Minxia Chou, Peng Shi, Gehong Wei
Summary: The study revealed that soil multifunctionality and microbial communities play a crucial role in regulating the dynamics of the peanut stem rot pathogen, Athelia rolfsii, providing insights into the relationships between soil factors, pathogen dynamics, and plant health.
Review
Microbiology
Elaina M. Blair, Katharine L. Dickson, Michelle A. O'Malley
Summary: This review discusses the recent progress and opportunities in cultivating natural and engineered microbial consortia for organic fraction of municipal solid waste (OFMSW) hydrolysis. It emphasizes the degradation of recalcitrant substrates by enzymes and the critical factors governing microbial interactions and culture stability. The need for increased standardization in measuring substrate degradation is also highlighted to enable comparisons across different environments.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Engineering, Chemical
Sven Annas, Michael Elfering, Hans-Arno Jantzen, Jurgen Scholz
Summary: The aim of this paper is to understand the mixing processes in biogas plants and explore optimization strategies for efficient mixing. By comparing different agitator positions, the risk of stagnation zones can be reduced and efficient mixing can be achieved. The study shows that complete mixing is possible at lower speeds with an optimal agitator position, resulting in improved energy efficiency.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Biochemical Research Methods
Kay Schallert, Pieter Verschaffelt, Bart Mesuere, Dirk Benndorf, Lennart Martens, Tim Van den Bossche
Summary: In metaproteomics, the protein inference problem is more challenging than in single-species proteomics. To address this issue, we developed a tool called Pout2Prot, which converts Percolator output files into protein group output files that can be used with Prophane.
JOURNAL OF PROTEOME RESEARCH
(2022)
Correction
Biochemical Research Methods
Rebeca Kawahara, Anastasia Chernykh, Kathirvel Alagesan, Marshall Bern, Weiqian Cao, Robert J. Chalkley, Kai Cheng, Matthew S. Choo, Nathan Edwards, Radoslav Goldman, Marcus Hoffmann, Yingwei Hu, Yifan Huang, Jin Young Kim, Doron Kletter, Benoit Liquet, Mingqi Liu, Yehia Mechref, Bo Meng, Sriram Neelamegham, Terry Nguyen-Khuong, Jonas Nilsson, Adam Pap, Gun Wook Park, Benjamin L. Parker, Cassandra L. Pegg, Josef M. Penninger, Toan K. Phung, Markus Pioch, Erdmann Rapp, Enes Sakalli, Miloslav Sanda, Benjamin L. Schulz, Nichollas E. Scott, Georgy Sofronov, Johannes Stadlmann, Sergey Y. Vakhrushev, Christina M. Woo, Hung-Yi Wu, Pengyuan Yang, Wantao Ying, Hui Zhang, Yong Zhang, Jingfu Zhao, Joseph Zaia, Stuart M. Haslam, Giuseppe Palmisano, Jong Shin Yoo, Goran Larson, Kai-Hooi Khoo, Katalin F. Medzihradszky, Daniel Kolarich, Nicolle H. Packer, Morten Thaysen-Andersen
Article
Chemistry, Analytical
Hermann J. Huette, Birgit Tiemann, Aleksandra Shcherbakova, Valerian Grote, Marcus Hoffmann, Lorenzo Povolo, Mark Lommel, Sabine Strahl, Sergey Y. Vakhrushev, Erdmann Rapp, Falk F. R. Buettner, Adnan Halim, Anne Imberty, Hans Bakker
Summary: A newly discovered lectin, BC2L-A, has been found to selectively enrich C- and O-mannosylated peptides, enabling the identification of novel C-mannosylation sites.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Jan Kuechler, Sebastian Puettker, Patrick Lahmann, Yvonne Genzel, Sascha Kupke, Dirk Benndorf, Udo Reichl
Summary: A mass spectrometry-based method was developed to perform absolute quantification of IAV proteins in infected MDCK cells, providing important insights into viral protein dynamics.
JOURNAL OF PROTEOMICS
(2022)
Article
Polymer Science
Gabriela Precup, Joachim Venus, Monika Heiermann, Roland Schneider, Ioana Delia Pop, Dan Cristian Vodnar
Summary: Xylo-oligosaccharides and fermentable sugars were extracted from wheat straw using an integrated approach. The combination of alkaline and enzymatic treatment with xylanase showed the highest concentration of fermentable sugars, while slightly reducing the yield of xylo-oligosaccharides. Refining experiments resulted in a purified fraction, but further optimization is needed to increase the yield.
Article
Microbiology
Alberto Scoma, Way Cern Khor, Marta Coma, Robert Heyer, Ruben Props, Tim Bouts, Dirk Benndorf, Desheng Li, Hemin Zhang, Korneel Rabaey
Summary: The gut microbiome of giant pandas plays a significant role in their nutrition by helping with digestion processes, and experimental results show that green leaves are mainly fermented into ethanol while yellow pith is mainly fermented into lactate. Further research is needed to understand the effects of pH, dung samples, fermentation products, and bamboo supply on pandas.
Article
Biochemistry & Molecular Biology
Robert Burock, Samanta Cajic, Rene Hennig, Falk F. R. Buettner, Udo Reichl, Erdmann Rapp
Summary: Glycosylation, especially N-glycosylation, is a common protein modification with significant importance in various fields. Accurate N-glycan analysis is essential, but challenging due to the complexity of the glycosylation process. Contamination with oligosaccharide impurities (OSIs) can further complicate the analysis.
Article
Medicine, Research & Experimental
Lucrezia Morticelli, Charlotte Rossdam, Samanta Cajic, Dietmar Boethig, Mikhail Magdei, Sugat Ratna Tuladhar, Bjoern Petersen, Konrad Fischer, Erdmann Rapp, Sotirios Korossis, Axel Haverich, Angelika Schnieke, Heiner Niemann, Falk F. R. Buettner, Andres Hilfiker
Summary: The study investigates the immunogenicity of porcine tissue in xenotransplantation by analyzing the glycome and glycosphingolipidome of porcine pericardia with different genetic deletions. The results show that the removal of specific epitopes changes the distribution and levels of other potentially immunogenic porcine glycans.
XENOTRANSPLANTATION
(2023)
Article
Engineering, Multidisciplinary
Samanta Cajic, Rene Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
Summary: As the roles of glycans in health and disease continue to be revealed, the complexity of glycan structures cannot be overlooked. A new integrative approach was developed to fully characterize glycan structures, bridging the gap between different glycoanalytical methods. The capabilities of this approach were demonstrated through detailed structural analysis of complex N-glycans and the discovery of new targets that were previously difficult to identify.
Article
Biotechnology & Applied Microbiology
Juliane Hahn, Paula Renate Westerman, Baerbel Gerowitt, Monika Heiermann
Summary: Through experiments, it was found that seeds may survive and contaminate the digestate during anaerobic digestion. This finding was verified in both lab-scale and full-scale reactors, and seed survival was tested in a pH-buffered water bath. The results showed that the commercial reactor was more effective in killing seeds compared to the experimental reactor and water bath treatments. While the risk of seed contamination can be reduced in non-hard-seeded species, it remains a challenge for hard-seeded species.
FERMENTATION-BASEL
(2023)
Article
Microbiology
Julia Hassa, Tom Jonas Tubbesing, Irena Maus, Robert Heyer, Dirk Benndorf, Mathias Effenberger, Christian Henke, Benedikt Osterholz, Michael Beckstette, Alfred Puehler, Alexander Sczyrba, Andreas Schlueter
Summary: This study used metagenomic and metaproteomic analyses to investigate the microbial communities in a full-scale biogas plant with three differently operated digesters. The results showed that these microbial communities had a significant impact on the abundance of enzymes and genes involved in the biogas process, and identified key microorganisms involved in different steps of anaerobic digestion. Further analysis of the highly abundant MAGs in all digesters indicated their potential in ensuring biogas process stability.
Article
Biotechnology & Applied Microbiology
Jan Kuechler, Katharina Willenbuecher, Elisabeth Reiss, Lea Nuss, Marius Conrady, Patrice Ramm, Ulrike Schimpf, Udo Reichl, Ulrich Szewzyk, Dirk Benndorf
Summary: The supplementation of lignocellulose-degrading enzymes can improve biogas production in industrial biogas plants. It is crucial to have reliable methods to assess the structural stability of these enzymes. A mass-spectrometric-based assay was established to monitor the structural integrity of enzymes during anaerobic digestion. The study found low structural stability of lignocellulose-degrading enzymes, consistent with previous observations on biogas content.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Hoda Khesali Aghtaei, Sebastian Puettker, Irena Maus, Robert Heyer, Liren Huang, Alexander Sczyrba, Udo Reichl, Dirk Benndorf
Summary: This study investigated the resilience of a hydrogenotrophic microbial community in a laboratory-scale biological methanation reactor to power oscillations. The results demonstrated that the microbial community had a high adaptive potential and the methane production rate quickly recovered after various on-off hydrogen feeding regimes. The study also revealed that discontinuous feeding strategies could be used to enrich methanogenic Archaea in the microbial community, which contributes to the design and improvement of biological methanation processes.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Julia Beimdiek, Rene Hennig, Robert Burock, Oliver Puk, Saskia Biskup, Erdmann Rapp, Anke Lesinski-Schiedat, Falk F. R. Buettner, Anibh M. Das
Summary: This study analyzed N-glycosylation in a patient with CDG-IIb and her family members, revealing aberrant N-glycans associated with CDG-IIb and changes in immune-related proteins. This has important implications for understanding the pathogenesis of CDG-IIb and the diagnosis and treatment of related diseases.
Article
Biochemistry & Molecular Biology
Guinevere S. M. Lageveen-Kammeijer, Erdmann Rapp, Deborah Chang, Pauline M. Rudd, Carsten Kettner, Joseph Zaia
Summary: The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative to standardize the reporting of glycoanalytical methods and to assess their reproducibility. They have published several reporting guidelines that describe the information that should be provided for glycoanalytical methods, and have now presented the first version of reporting guidelines for glyco(proteo)mics analysis by capillary electrophoresis (CE).