Article
Medicine, Research & Experimental
Ruslan Rust, Rebecca Z. Weber, Melanie Generali, Debora Kehl, Chantal Bodenmann, Daniela Uhr, Debora Wanner, Kathrin J. Zuercher, Hirohide Saito, Simon P. Hoerstrup, Roger M. Nitsch, Christian Tackenberg
Summary: In this study, transgene- and xeno-free neural progenitor cells (NPCs) were generated for potential clinical applications. These cells had stable gene expression, could be expanded, and spontaneously differentiated into mature neurons. This research presents a valuable tool for safe and effective cell therapies for brain injuries.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Immunology
Asuka Morizane
Summary: Parkinson's disease is the second most common neurodegenerative disease and a major target of cell therapies. Traditional cell therapies using aborted fetal tissue as donors face issues of donor material shortage, tissue quality instability, and ethical restrictions. However, advancements in stem cell technologies have allowed for the production of pluripotent stem cell-derived donor cells with unlimited scale, stable quality, and fewer ethical problems. Clinical trials of pluripotent stem cell-based therapies for Parkinson's disease have started based on positive results from non-clinical studies. This mini-review discusses practical considerations of stem cell-based therapies for Parkinson's disease, including different modes of transplantation and immune rejection.
INFLAMMATION AND REGENERATION
(2023)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yi-Ping Yang, Yu-Jer Hsiao, Kao-Jung Chang, Shania Foustine, Yu-Ling Ko, Yi-Ching Tsai, Hsiao-Yun Tai, Yu-Chieh Ko, Shih-Hwa Chiou, Tai-Chi Lin, Shih-Jen Chen, Yueh Chien, De-Kuang Hwang
Summary: Human pluripotent stem cells (PSCs) have the potential to replace diseased or injured tissues and are widely used in regenerative medicine. Induced pluripotent stem cells (iPSCs) can differentiate into various cell types and are being explored for the treatment of retinal diseases. Further clinical trials are needed to assess the efficacy and adverse effects of iPSC-based therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Tingting Xu, Xiaofei Li, Yuxi Guo, Elias Uhlin, Lena Holmberg, Sumonto Mitra, Dania Winn, Anna Falk, Erik Sundstrom
Summary: This study investigates the therapeutic effects of neuroepithelial-like stem cells (NESCs) derived from Good Manufacturing Practice (GMP)-compliant induced pluripotent stem cells (iPSCs) in a rat model of post-traumatic syringomyelia (PTS). The results show that off-the-shelf NESCs transplanted 10 weeks post-injury can reduce cyst volume and stimulate tissue repair, indicating their potential as a complement to standard surgery in PTS.
Article
Cell & Tissue Engineering
Yajie Gong, Haoyun Duan, Xin Wang, Can Zhao, Wenjing Li, Chunxiao Dong, Zongyi Li, Qingjun Zhou
Summary: Transplantation of hiPSC-derived NCCs can rapidly restore rabbit corneal thickness and clarity, but long-term recovery efficacy may be impaired by factors such as improper maturation, senescence, and endothelial-mesenchymal transition of the transplanted cells.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Ana Belen Alvarez-Palomo, Jordi Requena-Osete, Raul Delgado-Morales, Victoria Moreno-Manzano, Carme Grau-Bove, Agueda M. Tejera, Manel Juan Otero, Carme Barrot, Irene Santos-Barriopedro, Alejandro Vaquero, Jovita Mezquita-Pla, Sebastian Moran, Carlos Hobeich Naya, Iris Garcia-Martinez, Francisco Vidal Perez, Maria A. Blasco, Manel Esteller, Michael J. Edel
Summary: The study demonstrates that utilizing synthetic mRNA transfection of CYCLIN D1 during reprogramming repairs DNA and significantly improves the genetic stability of human iPSC. This method reduces various genetic instabilities, decreases DNA damage, promotes correct protein expression, and reduces single-nucleotide polymorphism changes per chromosome.
Review
Biochemistry & Molecular Biology
Agnieszka Fus-Kujawa, Barbara Mendrek, Anna Trybus, Karolina Bajdak-Rusinek, Karolina L. Stepien, Aleksander L. Sieron
Summary: Induced pluripotent stem cells (iPSCs) show potential in gene therapy and disease modeling. To safely apply this technology in clinical settings, optimal reprogramming factors and vectors must be considered to reduce the risk of insertional tumorigenesis.
Review
Biotechnology & Applied Microbiology
Wei Cheng, Chengming Fan, Qing Song, Ping Chen, Hong Peng, Ling Lin, Cong Liu, Bin Wang, Zijing Zhou
Summary: Fibrotic diseases are responsible for a third of global deaths, leading to organ remodeling and dysfunction. Despite the complications of organ transplantation, induced pluripotent stem cell (iPSC)-based therapy shows potential as a treatment option by replacing fibrotic organs. Recent studies have successfully differentiated iPSCs into fibrosis-relevant cell types with anti-fibrotic effects, providing promising precision treatments for organ-specific fibrosis. This review summarizes the potential, advantages, challenges, and future outlook of iPSC-based approaches in treating organ fibrosis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Li-Jie Hsu, Chao-Lin Liu, Ming-Ling Kuo, Chia-Ning Shen, Chia-Rui Shen
Summary: Cell therapy involves using cells selected, manipulated, and treated outside the body to treat or prevent human disease. iPSCs are ideal cell sources due to their unique characteristics. NK cells are effective tumor killers without causing GVHD, making them excellent candidates for cell therapy.
Review
Cell Biology
Yu Li, Ping-Ping Shen, Bin Wang
Summary: Spinal cord injury presents a significant challenge in trauma repair due to its difficulty and increasing morbidity. Stem cell therapy, particularly induced pluripotent stem cells, holds great potential in treating spinal cord injury by differentiating into precursor cells of various neural cell types at the injury site.
NEURAL REGENERATION RESEARCH
(2021)
Article
Cell Biology
Alexandra Neaverson, Malin H. L. Andersson, Osama A. Arshad, Luke Foulser, Mary Goodwin-Trotman, Adam Hunter, Ben Newman, Minal Patel, Charlotte Roth, Tristan Thwaites, Helena Kilpinen, Matthew E. Hurles, Andrew Day, Sebastian S. Gerety
Summary: Efficient and effective methods for converting human induced pluripotent stem cells into differentiated derivatives are critical for performing robust, large-scale studies of development and disease modelling, and for providing a source of cells for regenerative medicine. Here, we describe a 14-day neural differentiation protocol which allows for the scalable, simultaneous differentiation of multiple iPSC lines into cortical neural stem cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Tianyuan Shi, Martin Cheung
Summary: Using rodents for modeling neurological diseases has limitations due to differences in genetics, neural development, and physiology between humans and rodents. In the past decade, induced pluripotent stem cells and induced neural stem cells generated through reprogramming somatic cells have offered a powerful alternative for studying disease pathogenesis and testing regenerative medicines. Urine-derived stem cells are an ideal cell source for reprogramming due to their proliferation, multipotency, epithelial nature, and ease of reprogramming.
CELL AND BIOSCIENCE
(2021)
Review
Immunology
Ratchapong Netsrithong, Methichit Wattanapanitch
Summary: This review discusses the current status and future directions of CAR T cell therapy, exploring the potential of using iPSCs as a source of T cells and the genome engineering techniques for generating next-generation CAR T cells. It also highlights the challenges faced at present and the prospects for future universal off-the-shelf immunotherapeutic products.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Simona Gribaudo, Philippe Tixador, Luc Bousset, Alexis Fenyi, Patricia Lino, Ronald Melki, Jean-Michel Peyrin, Anselme L. Perrier
Article
Neurosciences
Sonia Lavisse, Susannah Williams, Sophie Lecourtois, Nadja van Camp, Martine Guillermier, Pauline Gipchtein, Caroline Jan, Sehastien Goutal, Leopold Eymin, Julien Valette, Thierry Delzescaux, Anselme L. Perrier, Philippe Hantraye, Romina Aron Badin
NEUROBIOLOGY OF DISEASE
(2019)
Article
Cell & Tissue Engineering
Marija Fjodorova, Morgane Louessard, Zongze Li, Daniel C. De La Fuente, Emma Dyke, Simon P. Brooks, Anselme L. Perrier, Meng Li
Article
Multidisciplinary Sciences
Romina Aron Badin, Aurore Bugi, Susannah Williams, Marta Vadori, Marie Michael, Caroline Jan, Alberto Nassi, Sophie Lecourtois, Antoine Blancher, Emanuele Cozzi, Philippe Hantraye, Anselme L. Perrier
NATURE COMMUNICATIONS
(2019)
Letter
Clinical Neurology
Anne E. Rosser, Monica Busse, Romina Aron Badin, Josep M. Canals, Vicki Wheelock, Anselme L. Perrier, William Gray, Leslie Thompson, Steven Goldman
MOVEMENT DISORDERS
(2021)
Article
Multidisciplinary Sciences
Amandine Virlogeux, Chiara Scaramuzzino, Sophie Lenoir, Remi Carpentier, Morgane Louessard, Aurelie Genoux, Patricia Lino, Maria-Victoria Hinckelmann, Anselme L. Perrier, Sandrine Humbert, Frederic Saudou
Summary: Increasing brain palmitoylation via ML348 shows therapeutic potential for Huntington disease by restoring BDNF transport and reversing neuropathology, locomotor deficits, and anxio-depressive behaviors in HD knock-in mice.
Review
Biochemistry & Molecular Biology
Juliette Duchesne de Lamotte, Anselme Perrier, Cecile Martinat, Camille Nicoleau
Summary: Botulinum neurotoxins (BoNTs) are deadly toxins produced by Clostridium botulinum, but are also used for cosmetic and therapeutic purposes. Engineering BoNTs can expand their applications and improve safety and clinical benefits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Julie Bigarreau, Nathalie Rouach, Anselme L. Perrier, Franck Mouthon, Mathieu Charveriat
Summary: Neuroglial interactions play a crucial role in the study of neurological disorders, and the discovery of human induced pluripotent stem cells has provided a new avenue for research in this field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Juliette Duchesne de Lamotte, Jerome Polentes, Florine Roussange, Lea Lesueur, Pauline Feurgard, Anselme Perrier, Camille Nicoleau, Cecile Martinat
Summary: This study developed a functional muscle-nerve coculture system using human-induced pluripotent stem cell-derived motor neurons and muscle cells for investigating the sensitivity to botulinum neurotoxins (BoNTs). Optogenetics combined with live calcium imaging was implemented to monitor the impact of BoNTs on synaptic transmission in a human motor endplate model, demonstrating potential for pharmaceutical BoNTs testing and development.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Clinical Neurology
Anne E. Rosser, Monica E. Busse, William P. Gray, Romina Aron Badin, Anselme L. Perrier, Vicki Wheelock, Emanuele Cozzi, Unai Perpina Martin, Cristina Salado-Manzano, Laura J. Mills, Cheney Drew, Steven A. Goldman, Josep M. Canals, Leslie M. Thompson
Summary: Substantial progress has been made in regenerative medicine strategies for CNS disorders, but there are challenges in translating these strategies to clinical settings, such as validating preclinical data, ensuring quality control of cell products, designing surgical devices, and conducting efficient clinical trials. Addressing these challenges in Huntington's disease can serve as a roadmap for other neurological conditions.
Meeting Abstract
Clinical Neurology
Sylvain Perriot, Amandine Mathias, Guillaume Perriard, Mathieu Canales, Nils Jonkmans, Nicolas Merienne, Cecile Meunier, Lina El Kassar, Anselme Perrier, David Laplaud, Myriam Schluep, Nicole Deglon, Renaud Du Pasquier
Meeting Abstract
Biotechnology & Applied Microbiology
N. Merienne, G. Vachey, K. Cambon, C. Meunier, V. Zimmer, G. Perriard, M. Canales, A. Mathias, C. Pythoud, M. Rey, L. Pellerin, E. Brouillet, A. Perrier, R. Du Pasquier, N. Deglon
HUMAN GENE THERAPY
(2019)
Meeting Abstract
Biotechnology & Applied Microbiology
Y. Arsenijevic, S. Decembrini, R. Moser, S. Hoehnel, N. Brandenberg, D. Gamm, V. Ponce de Leone, M. Lutolf
HUMAN GENE THERAPY
(2018)
Meeting Abstract
Clinical Neurology
Julie Bigarreau, Barbara Baldo, Johana Toumois, Morgane Louessard, Margot Jarrige, Gilles Bonvento, Marie Michael, Gurvan Mahe, Asa Petersen, Deniz Kirik, Anselme L. Perrier
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2018)
Meeting Abstract
Clinical Neurology
Nicole Deglon, Gabriel Vachey, Maria Rey, Anselme Perrier
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2018)