Cryo-EM structures of the human volume-regulated anion channel LRRC8

Structure of a volume-regulated anion channel of the LRRC8 family

(2018) Dawid Deneka et al.   NATURE

Subunit-dependent oxidative stress sensitivity of LRRC8 volume-regulated anion channels

(2017) Antonella Gradogna et al.   JOURNAL OF PHYSIOLOGY-LONDON

Molecular composition and heterogeneity of the LRRC8-containing swelling-activated osmolyte channels in primary rat astrocytes

(2017) Alexandra L. Schober et al.   JOURNAL OF PHYSIOLOGY-LONDON

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

Improvements to the APBS biomolecular solvation software suite

(2017) Elizabeth Jurrus et al.   PROTEIN SCIENCE

LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense Ionic Strength

(2016) Ruhma Syeda et al.   CELL

Inactivation and Anion Selectivity of Volume-regulated Anion Channels (VRACs) Depend on C-terminal Residues of the First Extracellular Loop

(2016) Florian Ullrich et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

VRACs and other ion channels and transporters in the regulation of cell volume and beyond

(2016) Thomas J. Jentsch   NATURE REVIEWS MOLECULAR CELL BIOLOGY

An electrostatic mechanism for Ca2+-mediated regulation of gap junction channels

(2016) Brad C. Bennett et al.   Nature Communications

Atomic structure of the innexin-6 gap junction channel determined by cryo-EM

(2016) Atsunori Oshima et al.   Nature Communications

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

(2016) Dari Kimanius et al.   eLife

The identification of a volume-regulated anion channel: an amazing Odyssey

(2015) S. F. Pedersen et al.   Acta Physiologica

The volume-regulated anion channel is formed by LRRC8 heteromers – molecular identification and roles in membrane transport and physiology

(2015) Tobias Stauber   BIOLOGICAL CHEMISTRY

Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, cancer and drug resistance

(2015) Else K Hoffmann et al.   Channels

Subunit composition of VRAC channels determines substrate specificity and cellular resistance to Pt-based anti-cancer drugs

(2015) R. Planells-Cases et al.   EMBO JOURNAL

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Semi-automated selection of cryo-EM particles in RELION-1.3

(2015) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

The Phyre2 web portal for protein modeling, prediction and analysis

(2015) Lawrence A Kelley et al.   Nature Protocols

SWELL1, a Plasma Membrane Protein, Is an Essential Component of Volume-Regulated Anion Channel

(2014) Zhaozhu Qiu et al.   CELL

Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

(2014) April Goehring et al.   Nature Protocols

Identification of LRRC8 Heteromers as an Essential Component of the Volume-Regulated Anion Channel VRAC

(2014) F. K. Voss et al.   SCIENCE

LRRC8 proteins share a common ancestor with pannexins, and may form hexameric channels involved in cell-cell communication

(2012) Federico Abascal et al.   BIOESSAYS

A Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Protein Precrystallization Screening

(2012) Motoyuki Hattori et al.   STRUCTURE

Osmosensory Mechanisms in Cellular and Systemic Volume Regulation

(2011) S. F. Pedersen et al.   JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

ConSurf 2010: calculating evolutionary conservation in sequence and structure of proteins and nucleic acids

(2010) H. Ashkenazy et al.   NUCLEIC ACIDS RESEARCH

Structure of the connexin 26 gap junction channel at 3.5 Å resolution

(2009) Shoji Maeda et al.   NATURE

Modulation of protein properties in living cells using nanobodies

(2009) Axel Kirchhofer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Physiology of Cell Volume Regulation in Vertebrates

(2009) Else K. Hoffmann et al.   PHYSIOLOGICAL REVIEWS

Rapid and improved reconstitution of bacterial mechanosensitive ion channel proteins MscS and MscL into liposomes using a modified sucrose method

(2008) Andrew R. Battle et al.   FEBS LETTERS