Article
Biology
Gina M. LoMastro, Chelsea G. Drown, Aubrey L. Maryniak, Cayla E. Jewett, Margaret A. Strong, Andrew Jon Holland
Summary: Multiciliated cells assemble multiple motile cilia through centriole amplification and surface area expansion. Recent research has shown that Polo-like kinase 4 plays a critical role in centriole amplification in multiciliated cells.
Article
Cell Biology
Moe R. Mahjoub, Rashmi Nanjundappa, Megan N. Harvey
Summary: This review discusses recent progress in understanding the mechanisms of centriole and cilia biogenesis in multiciliated cells (MCC), and explores how MCC progenitor cells regulate the number of centrioles and cilia during their differentiation.
CURRENT OPINION IN CELL BIOLOGY
(2022)
Review
Cell Biology
Qian Lyu, Qingchao Li, Jun Zhou, Huijie Zhao
Summary: This review summarizes the current knowledge of multiciliogenesis and highlights recent advances in understanding the regulation of multiple motile cilia formation.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Developmental Biology
Sun K. Kim, Eva Brotslaw, Virginie Thome, Jen Mitchell, Rosa Ventrella, Caitlin Collins, Brian Mitchell
Summary: Centriole amplification in multiciliated cells is regulated by a process involving the deuterosome, with the centrosomal protein Cep70 identified as a novel regulator. Cep70 associates with deuterosomes during centriole biogenesis, and depletion of Cep70 leads to a decrease in centriole formation and defects in radial intercalation in multiciliated cells. This suggests that Cep70 plays a crucial role in regulating centriole biogenesis in multiciliated cells.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Saurabh Kulkarni, Jonathan Marquez, Priya Date, Rosa Ventrella, Brian J. Mitchell, Mustafa K. Khokha
Summary: Cells regulate the number of cilia and centrioles in multiciliated cells (MCCs) through mechanical forces, centrioles, and the mechanosensitive ion channel Piezo1, ensuring optimal cell function. This study provides new insights into organelle number control in cells.
Article
Multidisciplinary Sciences
Seongjae Kim, Yuan-Hung Chien, Amy Ryan, Chris Kintner
Summary: Through this study, an emi2-APC/C-plk1 axis was identified to be crucial for the assembly and termination of centriole and basal body formation during multiciliated cell differentiation.
Article
Cell Biology
Aude Nommick, Camille Boutin, Olivier Rosnet, Claire Schirmer, Elsa Bazellieres, Virginie Thome, Etienne Loiseau, Annie Viallat, Laurent Kodjabachian
Summary: Ciliated epithelia play essential roles in animals, from locomotion to mucociliary clearance. This study reveals the crucial functions of the coiled-coil domain proteins Lrrcc1 and Ccdc61 in basal body biogenesis and organization, highlighting their importance for the physiological function of ciliated epithelia.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Michael Lewis, Travis H. Stracker
Summary: The differentiation program of multiciliated cells is initiated by GEMC1 and MCIDAS, which activate key transcription factors to control the generation of multiple motile cilia. To support this, massive centriole amplification is required, along with the generation of unique deuterosomes and extensive remodeling of the cytoskeleton.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jose Gonzalez-Martinez, Andrzej W. Cwetsch, Javier Gilabert-Juan, Jesus Gomez, Guillermo Garaulet, Paulina Schneider, Guillermo de Carcer, Francisca Mulero, Eduardo Caleiras, Diego Megias, Eva Porlan, Marcos Malumbres
Summary: This study reveals that the centrosomal kinase PLK1 regulates centrosome asymmetry and cell fate in neural progenitors. Loss of PLK1 activity leads to reduced asymmetry and increased expansion of neural progenitors, promoting cortical growth. However, deficiencies in MCPH proteins result in increased centrosome asymmetry and microcephaly.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Multidisciplinary Sciences
Guilherme Ventura, Aboutaleb Amiri, Raghavan Thiagarajan, Mari Tolonen, Amin Doostmohammadi, Jakub Sedzinski
Summary: During embryonic development, cells migrate and integrate into target tissues to execute their function. This research reveals that multiciliated cell precursors in Xenopus embryo use filopodia to sense vertex stiffness and remodel epithelial junctions of neighboring cells, facilitating integration.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Jose Gonzalez-Martinez, Andrzej W. Cwetsch, Diego Martinez-Alonso, Luis R. Lopez-Sainz, Jorge Almagro, Anna Melati, Jesus Gomez, Manuel Perez-Martinez, Diego Megias, Jasminka Boskovic, Javier Gilabert-Juan, Osvaldo Grana-Castro, Alessandra Pierani, Axel Behrens, Sagrario Ortega, Marcos Malumbres
Summary: The study reveals that defects in certain genes during development can lead to mild MCPH symptoms, while the lack of centrosome or centriole regulators can cause chromosomal instability in neural progenitor cells.
Article
Biochemical Research Methods
Marco Antonio Dias Louro, Monica Bettencourt-Dias, Claudia Bank
Summary: This study investigates the phenomenon of centrosome amplification in cancer cells, where excess centrioles are common. Surprisingly, the study finds that the variability in centriole numbers in most cell lines can be best explained by models assuming a single penalty against extra centrioles, regardless of their number. Furthermore, the study estimates that selection against extra centrioles is generally very strong.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Cell Biology
Franz Meitinger, Dong Kong, Midori Ohta, Arshad Desai, Karen Oegema, Jadranka Loncarek
Summary: TRIM37 plays a role in centrosomes by preventing the assembly of centrobin-scaffolded condensates, ensuring accurate chromosome segregation. Mutations in TRIM37 cause mulibrey nanism, with patient-derived cells showing ectopic poles organized by centrobin condensates, suggesting chromosome missegregation as a pathological mechanism in this disorder.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lauren T. Evans, Taylor Anglen, Phillip Scott, Kimberly Lukasik, Jadranka Loncarek, Andrew J. Holland
Summary: The study reveals that centriole distal appendages play a critical role in PIDDosome activation in cells with extra centrosomes. ANKRD26 interacts with and recruits PIDD1 to centriole distal appendages, and this interaction is essential for PIDDosome activation following centrosome amplification. These findings suggest a model where ANKRD26 initiates a centriole-derived signal to limit cell proliferation in response to centrosome amplification.
Article
Multidisciplinary Sciences
Remy Robinot, Mathieu Hubert, Guilherme Dias de Melo, Francoise Lazarini, Timothee Bruel, Nikaia Smith, Sylvain Levallois, Florence Larrous, Julien Fernandes, Stacy Gellenoncourt, Stephane Rigaud, Olivier Gorgette, Catherine Thouvenot, Celine Trebeau, Adeline Mallet, Guillaume Dumenil, Samy Gobaa, Raphael Etournay, Pierre-Marie Lledo, Marc Lecuit, Herve Bourhy, Darragh Duffy, Vincent Michel, Olivier Schwartz, Lisa A. Chakrabarti
Summary: Understanding how SARS-CoV-2 spreads within the respiratory tract is crucial in determining the severity of COVID-19. This study shows that SARS-CoV-2 infection leads to rapid loss of airway motile cilia, affecting cilia clearance function, with reduced expression of the ciliogenesis regulator Foxj1 preceding cilia loss. Further analysis in Syrian hamsters confirms the loss of motile cilia in vivo.
NATURE COMMUNICATIONS
(2021)
Review
Oncology
Kuo-Tai Yang, Chieh-Ju C. Tang, Tang K. Tang
FRONTIERS IN ONCOLOGY
(2015)
Article
Cell Biology
Ching-Wen Chang, Wen-Bin Hsu, Jhih-Jie Tsai, Chieh-Ju C. Tang, Tang K. Tang
JOURNAL OF CELL SCIENCE
(2016)
Article
Cell Biology
En-Ju Chou, Liang-Yi Hung, Chieh-Ju C. Tang, Wen-Bin Hsu, Hsin-Yi Wu, Pao-Chi Liao, Tang K. Tang
Article
Biochemistry & Molecular Biology
Zhen-Hua Wang, Yen-Yu Chang, Jhih-Guang Wu, Chia-Yu Lin, Hsiao-Lung An, Shyh-Chyang Luo, Tang K. Tang, Wei-Fang Su
MACROMOLECULAR BIOSCIENCE
(2018)
Article
Multidisciplinary Sciences
Hsin-Yi Chen, Chien-Ting Wu, Chieh-Ju C. Tang, Yi-Nan Lin, Won-Jing Wang, Tang K. Tang
NATURE COMMUNICATIONS
(2017)
Article
Cell Biology
Chien-Ting Wu, Hsin-Yi Chen, Tang K. Tang
NATURE CELL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Hui-Fang Chang, Ying-Shan Lee, Tang K. Tang, Ji-Yen Cheng
Article
Multidisciplinary Sciences
Jhih-Jie Tsai, Wen-Bin Hsu, Jia-Hua Liu, Ching-Wen Chang, Tang K. Tang
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Yi-Nan Lin, Ying-Shan Lee, Shu-Kuei Li, Tang K. Tang
JOURNAL OF CELL SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Ting-Yu Chen, Bu-Miin Huang, Tang K. Tang, Yu-Ying Chao, Xiao-Yi Xiao, Pei-Rong Lee, Li-Yun Yang, Chia-Yih Wang
Summary: The DNA-PK-p53 cascade induces primary cilia formation under genotoxic stress, contributing to the DNA damage response, while lack of this pathway decreases genotoxic stress-induced ciliogenesis. Besides, ciliogenesis is regulated not only by DNA-PK, but also by autophagy.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Cell Biology
En-Ju Chou, Tang K. Tang
Summary: The study highlighted the important role of RTTN in mitosis, showing that its depletion can lead to various mitotic abnormalities, including abnormal spindle formation and dispersion of pericentriolar proteins.
Article
Cell Biology
Chia-Hsiang Chang, Ting-Yu Chen, I-Ling Lu, Rong-Bin Li, Jhih-Jie Tsai, Pin-Yeh Lin, Tang K. Tang
Summary: Chang et al. report that CEP120, a JS-associated protein involved in centriole biogenesis and cilia assembly, regulates timely neuronal differentiation and the departure of granule neuron progenitors (GNPs) from their germinal zone during cerebellar development. Their findings reveal a close interplay between CEP120 and KIAA0753 for the germinal zone exit and timely neuronal differentiation of GNPs during cerebellar development, and mutations in CEP120 and KIAA0753 may participate in the heterotopia and cerebellar hypoplasia observed in JS patients.
GENES & DEVELOPMENT
(2021)
Article
Cell Biology
Hsiao-Lung An, Hung-Chih Kuo, Tang K. Tang
Summary: Researchers generated human induced pluripotent stem cells (hiPSCs) carrying a mutant centrosomal protein using CRISPR/Cas9 gene editing technology. The mutant protein disrupted the recruitment of centriolar proteins involved in centriole elongation and resulted in the production of short centrioles and long cilia. Additionally, the mutant protein induced neuronal cell death and premature neuronal differentiation, leading to smaller brain organoids resembling the phenotype of microcephaly. These findings provide insights into the pathogenic mechanisms of MCPH.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Yi-Wei Wang, Shu-Chuan Chen, De-Leung Gu, Yi-Chen Yeh, Jhih-Jie Tsai, Kuo-Tai Yang, Yuh-Shan Jou, Teh-Ying Chou, Tang K. Tang
Summary: The study investigates the expression levels of centriolar/centrosomal genes in various types of cancers and identifies STIL as a protein that is highly expressed in lung and other types of cancers. Depletion of STIL inhibits tumor growth and metastasis, while excess STIL activates the EMT pathway and enhances cancer cell migration and invasion. The study reveals an unexpected role of STIL in tumor metastasis and identifies its association with FOXM1 in promoting metastasis and stemness.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ana L. Alvarez-Cabrera, Sandra Delgado, David Gil-Carton, Gulnahar B. Mortuza, Guillermo Montoya, Carlos O. S. Sorzano, Tang K. Tang, Jose M. Carazo
FRONTIERS IN MOLECULAR BIOSCIENCES
(2017)
