Article
Microbiology
Mariusz Madej, Zuzanna Nowakowska, Miroslaw Ksiazek, Anna M. Lasica, Danuta Mizgalska, Magdalena Nowak, Anna Jacula, Monika Bzowska, Carsten Scavenius, Jan J. Enghild, Joseph Aduse-Opoku, Michael A. Curtis, F. Xavier Gomis-Ruth, Jan Potempa
Summary: The T9SS component PorZ interacts with sortase and specifically binds A-LPS, mediated by a phosphorylated branched mannan repeat in A-LPS polysaccharide. ALPS-bound PorZ interacts with sortase with significantly higher affinity, facilitating modification of cargo proteins by the cell surface attachment complex of the T9SS.
Article
Microbiology
Anna Gasiorek, Ewelina Dobosz, Barbara Potempa, Izabela Ciaston, Mateusz Wilamowski, Zuzanna Oruba, Richard J. Lamont, Jolanta Jura, Jan Potempa, Joanna Koziel
Summary: Periodontal disease is an inflammatory disease of the supporting tissues of the teeth caused by dysbiotic biofilm on the tooth surface. The negative regulator MCPIP-1 plays a key role in controlling inflammatory responses, but its stability can be influenced by periodontal pathogens like Porphyromonas gingivalis. The proteolytic activity of gingipains from these pathogens can lead to rapid degradation of MCPIP-1, exacerbating the inflammatory response. This provides insights into potential new treatment strategies for periodontitis.
Article
Immunology
Sejal Patel, Derek Howard, Nityananda Chowdhury, Casey Derieux, Bridgette Wellslager, Ozlem Yilmaz, Leon French
Summary: Porphyromonas gingivalis, a bacterium associated with periodontal disease, is suspected to be linked to the development of Alzheimer's disease. Research on gingipain proteases and differentially expressed genes in brain samples with detected P. gingivalis sequences have revealed potential connections to periodontal disease, Alzheimer's disease, and dementia. Analysis of gene expression in mice and humans has highlighted specific neuroanatomical patterns related to P. gingivalis infection and its possible association with Alzheimer's disease.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Alex A. Knowles, Susan G. Campbell, Neil A. Cross, Prachi Stafford
Summary: This study investigated the molecular mechanisms by which P. gingivalis infection alters translation attenuation during oxidative stress-induced activation of the ISR in oral epithelial cells, using immunofluorescence confocal microscopy and immunoblotting. The findings revealed that infection coupled with stress caused differential stress granule formation and composition, and heightened stress-induced translational repression independently of core ISR mediators. The study also implicated the gingipain-mTOR axis as the effector of host translational dysregulation during stress.
Article
Microbiology
Izabela Ciaston, Joanna Budziaszek, Dorota Satala, Barbara Potempa, Andrew Fuchs, Maria Rapala-Kozik, Danuta Mizgalska, Ewelina Dobosz, Richard J. Lamont, Jan Potempa, Joanna Koziel
Summary: The study found that inactive gingipains still elicit a strong inflammatory response in gingival tissues, which may contribute to the progression of periodontitis and bone resorption. Moreover, host molecules were identified as receptors for proteolytically inactivated gingipains. The broad distribution of these receptors in human tissues suggests their involvement in systemic diseases associated with periodontal pathogens.
Article
Microbiology
Zhaolei Zou, Juan Fang, Wanting Ma, Junyi Guo, Zhongyan Shan, Da Ma, Qiannan Hu, Liling Wen, Zhi Wang
Summary: In this study, it was found that gingipains from P. gingivalis can degrade PECAM-1 to facilitate bacterial penetration and at the same time reduce leukocyte TEM capacity. The role of gingipains in modulating vascular barrier permeability and TEM processes provides a new understanding of the distal colonization of P. gingivalis and its associated systemic diseases.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Multidisciplinary
Eng Sze Wei, Ramasamy Kavitha, Mohammad Auwal Sa'ad, Pattabhiraman Lalitha, Neeraj Kumar Fuloria, Manickam Ravichandran, Shivkanya Fuloria
Summary: The isolation and characterization of gingipains from the periodontitis-causing bacterium Porphyromonas gingivalis is important for studying its invasion mechanism and potential links to periodontitis, Alzheimer's disease, and cardiovascular disease. This study introduces simpler methods using passive-mediated diffusion gel elution and gelatin zymography for the isolation and characterization of gingipains, which are easier and more cost-effective than traditional chromatographic processes and molecular cloning.
APPLIED SCIENCES-BASEL
(2023)
Editorial Material
Biochemistry & Molecular Biology
George Hajishengallis
Summary: The study using a zebrafish larvae infection model found that Porphyromonas gingivalis may cause vascular damage and increased endothelial permeability by degrading key proteins, leading to endothelial dysfunction and potentially atherosclerotic cardiovascular disease.
Article
Medicine, Research & Experimental
Sharon Kim, Yasuhiko Bando, Chungyu Chang, Jeonga Kwon, Berta Tarverti, Doohyun Kim, Sung Hee Lee, Hung Ton-That, Reuben Kim, Peter L. Nara, No-Hee Park
Summary: This study successfully induced local infection, including periodontitis and systemic vascular inflammation, by topically applying Pg into the gingival pockets of wild-type mice. Pathohistological changes included Pg colonization, increased gingival pocket depths, gingival inflammation, alveolar bone loss, and increased levels of pro-inflammatory cytokines in the serum.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Microbiology
Nanami Yoshino, Tsuyoshi Ikeda, Ryoma Nakao
Summary: The study found that fennel has significant activity against Porphyromonas gingivalis, a major pathogen in periodontitis. Fennel extracts showed rapid lethal action against P. gingivalis and minimal effects on other oral bacteria. The extracts formed protruding nanostructures and induced overproduction of outer membrane vesicles. Fennel also inhibited the activity of gingipain enzymes produced by P. gingivalis, preventing damage to oral epithelial cells. Petroselinic acid was identified as the major bactericidal and gingipain-inhibiting component of fennel.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Ingar Olsen, Sim K. Singhrao
Summary: Recent reports suggest that reduced levels of salivary lactoferrin may be linked to amyloid beta accumulation in the brains of Alzheimer's disease patients. This could potentially trigger oral dysbiosis, leading to changes in the oral microbiota and contributing to the development of AD pathophysiology.
MEDICAL HYPOTHESES
(2021)
Article
Microbiology
Marines du Teil Espina, Yanyan Fu, Demi van der Horst, Claudia Hirschfeld, Marina Lopez-Alvarez, Lianne M. Mulder, Costanza Gscheider, Anna Haider Rubio, Minke Huitema, Doerte Becher, Peter Heeringa, Jan Maarten van Dijl
Summary: P. gingivalis manipulates human neutrophils using outer membrane vesicles (OMVs), provoking degranulation without neutrophil killing, and OMVs can be internalized by macrophages and epithelial cells.
MICROBIOLOGY SPECTRUM
(2022)
Article
Neurosciences
Shalini Kanagasingam, Christopher von Ruhland, Richard Welbury, Sim K. Singhrao
Summary: The phosphorylated tau peptide A showed significant antimicrobial properties against P. gingivalis. CD spectroscopy revealed that both phosphorylated and non-phosphorylated peptide A mainly adopted beta-sheet structures in aqueous solution with potential antimicrobial activity. Phosphorylation of tau peptides physically changed their tertiary structure into PHFs, potentially leading to self-aggregation and binding to the NFT lesion.
JOURNAL OF ALZHEIMERS DISEASE
(2022)
Article
Pharmacology & Pharmacy
Meshal S. Alharbi, Fahad A. Alshehri
Summary: This study evaluated the effects of hyaluronic acid, azithromycin, and chlorhexidine on the virulence and adhesion-related genes of Porphyromonas gingivalis. The results showed that high molecular weight hyaluronic acid has a strong downregulating effect on the gene expression of P. gingivalis.
Article
Dentistry, Oral Surgery & Medicine
Zuzanna Nowakowska, Mariusz Madej, Sylwia Grad, Tiansong Wang, Murray Hackett, Daniel P. Miller, Richard J. Lamont, Jan Potempa
Summary: The phosphorylated proteome of the anaerobic bacterium Porphyromonas gingivalis mainly consists of phosphotyrosine residues, affecting major virulence factors and maturation pathways. Phosphorylation of specific domains in key proteins influences secretion, processing, and enzymatic activity, suggesting multiple phosphorylation-dependent pathways of gingipain maturation in P. gingivalis. The role of candidate kinases like Ptk1 BY kinase and Ubiquitous bacterial kinase 1 (UbK1) in gingipain processing and activation remains to be confirmed.
MOLECULAR ORAL MICROBIOLOGY
(2021)
Review
Immunology
Marcin Okroj, Jan Potempa
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Rheumatology
M. K. Jonsson, T. Kantyka, K. Falkowski, A. Aliko, A. B. Aga, S. Lillegraven, J. Sexton, B. T. Fevang, P. Mydel, E. A. Haavardsholm
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY
(2020)
Article
Immunology
Leonie M. Vogt, Ewa Kwasniewicz, Simone Talens, Carsten Scavenius, Ewa Bielecka, Kristina N. Ekdahl, Jan J. Enghild, Matthias Morgelin, Tore Saxne, Jan Potempa, Anna M. Blom
JOURNAL OF IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Katherine Falkowski, Ewa Bielecka, Ida B. Thogersen, Oliwia Bochenska, Karolina Plaza, Magdalena Kalinska, Laura Sasiadek, Malgorzata Magoch, Aleksandra Pecak, Magdalena Wisniewska, Natalia Gruba, Magdalena Wysocka, Anna Wojtysiak, Magdalena Brzezinska-Bodal, Kamila Sychowska, Anastasija Pejkovska, Maren Rehders, Georgina Butler, Christopher M. Overall, Klaudia Brix, Grzegorz Dubin, Adam Lesner, Andrzej Kozik, Jan J. Enghild, Jan Potempa, Tomasz Kantyka
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Microbiology
Maja Sochalska, Magdalena B. Stanczyk, Maria Uzarowska, Natalia Zubrzycka, Susanne Kirschnek, Aleksander M. Grabiec, Tomasz Kantyka, Jan Potempa
Article
Biochemistry & Molecular Biology
Danuta Bryzek, Anna Golda, Joanna Budziaszek, Dominik Kowalczyk, Alicia Wong, Ewa Bielecka, Priyanka Shakamuri, Pavel Svoboda, Jan Pohl, Jan Potempa, Joanna Koziel
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Aleksandra Milewska, Katherine Falkowski, Magdalena Kulczycka, Ewa Bielecka, Antonina Naskalska, Pawel Mak, Adam Lesner, Marek Ochman, Maciej Urlik, Elftherios Diamandis, Ioannis Prassas, Jan Potempa, Tomasz Kantyka, Krzysztof Pyrc
Article
Cell Biology
Ewelina Dobosz, Georg Lorenz, Andrea Ribeiro, Vivian Wuerf, Marta Wadowska, Jerzy Kotlinowski, Christoph Schmaderer, Jan Potempa, Mingui Fu, Joanna Koziel, Maciej Lech
Summary: Research has shown that depletion of MCPIP1 in macrophages and granulocytes can trigger severe autoimmune diseases like systemic lupus, affecting adaptive immune responses. This phenomenon is related to systemic autoinflammation and suggests that abnormal macrophage activation plays a significant role in the loss of immune tolerance.
DISEASE MODELS & MECHANISMS
(2021)
Article
Biochemistry & Molecular Biology
Malgorzata Miastkowska, Tomasz Kantyka, Ewa Bielecka, Urszula Kalucka, Marta Kaminska, Malgorzata Kucharska, Anna Kilanowicz, Dariusz Cudzik, Krzysztof Cudzik
Summary: The study compared the composition and proregenerative properties of commercially available French narrow-leaved lavender oil and locally cultivated Polish narrow-leaved lavender oil, finding that the self-manufactured oil had lower linalool content and higher lavandulyl acetate content. The lavender oils showed a concentration-dependent effect on IL-6, IL-8, and VEGF production by keratinocyte cell line, with the in-house preparation performing significantly better in increasing VEGF production in vitro cell models.
Article
Microbiology
Kathrin Stelzner, Aziza Boyny, Tobias Hertlein, Aneta Sroka, Adriana Moldovan, Kerstin Paprotka, David Kessie, Helene Mehling, Jan Potempa, Knut Ohlsen, Martin J. Fraunholz, Thomas Rudel
Summary: The bacterial cysteine protease staphopain A contributes to host cell death mediated by intracellular S. aureus, enhancing both bacterial survival in lung infection and tissue destruction. In epithelial cells, staphopain A induces cell death and intracellular replication without requiring bacterial proliferation. The role of staphopain A in facilitating colonization and dissemination of infection is highlighted in this study.
Article
Multidisciplinary Sciences
Liqiang Song, John D. Perpich, Chenggang Wu, Thierry Doan, Zuzanna Nowakowska, Jan Potempa, Peter J. Christie, Eric Cascales, Richard J. Lamont, Bo Hu
Summary: This study reveals the structure and assembly of P. gingivalis T9SS and its correlation with the development of periodontal disease. The T9SS shows unique structure and mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Katarzyna Szczesniak, Florian Veillard, Carsten Scavenius, Kamila Chudzik, Kinga Ferenc, Matthias Bochtler, Jan Potempa, Danuta Mizgalska
Summary: In certain bacteria, the glutaminyl cyclase (QC) is crucial for protein stability and bacterial growth. QC converts glutamine residues to pyroglutamate, protecting proteins from degradation. However, the loss of QC results in the loss of Q-rule substrates, leading to the inability of bacteria to survive.
Article
Biochemistry & Molecular Biology
Zsombor Prucsi, Agnieszka Zimny, Alicja Plonczynska, Natalia Zubrzycka, Jan Potempa, Maja Sochalska
Summary: Periodontitis is a chronic inflammatory disease caused by dysbiotic oral microbiome, and Porphyromonas gingivalis is identified as a key pathogen. The mechanism by which P. gingivalis secretes the enzyme PPAD to contribute to the inflammation loop is not fully understood. This study reveals that PPAD promotes neutrophil survival and pro-inflammatory cytokine secretion, while enhancing the resilience of bacteria to phagocytosis, thus sustaining a chronic inflammatory state.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Krzysztof M. Zak, Mark J. Bostock, Irena Waligorska, Ida B. Thogersen, Jan J. Enghild, Grzegorz M. Popowicz, Przemyslaw Grudnik, Jan Potempa, Miroslaw Ksiazek
Summary: Mirolysin, a secretory protease of Tannerella forsythia, achieves latency through a cysteine-switch mechanism exerted by Cys23. Mutation of Cys23 shortens the activation time, and a small molecule compound (cpd 9) functions as a competitive inhibitor blocking promirolysin maturation.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)