Article
Biochemistry & Molecular Biology
Allison M. Murawski, Mark P. Brynildsen
Summary: Genetic mutants have demonstrated the importance of homologous recombination (HR) to fluoroquinolone (FQ) persistence, suggesting that different types of persister subtypes exist, with some proficient in HR. Furthermore, time-lapse microscopy revealed distinct growth dynamics during the post-antibiotic recovery period for persisters from monoploid-enriched and diploid-enriched subpopulations.
Article
Multidisciplinary Sciences
Natalie Schindler, Matthias Tonn, Vanessa Kellner, Jia Jun Fung, Arianna Lockhart, Olga Vydzhak, Thomas Juretschke, Stefanie Moeckel, Petra Beli, Anton Khmelinskii, Brian Luke
Summary: The study reveals a genetic network involved in repairing the single ribonucleoside monophosphates (rNMPs) induced lesions and highlights its importance in human pathologies. This could potentially be utilized in the treatment of RNase H2-deficient pathologies.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Jeremy W. W. Schroeder, Rebecca L. L. Hurto, Justin R. R. Randall, Katherine J. J. Wozniak, Taylor A. A. Timko, Taylor M. M. Nye, Jue D. D. Wang, Peter L. L. Freddolino, Lyle A. A. Simmons
Summary: RNA:DNA hybrids hinder replication fork progression and compromise genome integrity in all cells. The impact of naturally occurring hybrids on genome integrity and the role of ribonucleases H in mitigating their negative effects are unknown. This study investigates the contributions of RNases HII and HIII to hybrid removal, DNA replication, and mutagenesis across the genome. Deletion of rnhB or rnhC leads to RNA:DNA hybrid accumulation with different patterns of mutagenesis and hybrid distribution. Hybrids mainly accumulate in noncoding RNAs and 5'-UTRs of coding sequences. In ?rnhB cells, hybrids preferentially accumulate in untranslated regions and early in coding sequences. Gene expression levels significantly affect hybrid accumulation in ?rnhC cells. Disruption of DNA replication in ?rnhC cells leads to transversions and structural variation. These findings provide insights into how hybrids cause mutagenesis and shape genome organization in native genomic contexts.
Article
Multidisciplinary Sciences
Qian Mei, Devon M. Fitzgerald, Jingjing Liu, Jun Xia, John P. Pribis, Yin Zhai, Ralf B. Nehring, Jacob Paiano, Heyuan Li, Andre Nussenzweig, P. J. Hastings, Susan M. Rosenberg
Summary: Chromosomal fragile sites are implicated in promoting genome instability, and this study identifies three spontaneous fragile sites in the Escherichia coli genome, revealing their DNA damage and repair mechanisms. The findings suggest that mechanisms such as replication fork collapse and shearing of unsegregated sister chromosomes may occur universally, including in humans, and play a role in diseases of genome instability such as somatic cell mosaicism and cancers.
Article
Microbiology
Steven Dunn, Laura Carrilero, Michael Brockhurst, Alan McNally
Summary: The study found that different E. coli strains vary in their ability to acquire and maintain MDR plasmids, with highly strain-specific transcriptional responses observed following plasmid acquisition. However, the subtle transcriptional responses consistent across all strains suggest that fitness costs arising from transcriptional disruption are unlikely to act as a barrier to dissemination of this MDR plasmid in E. coli.
Article
Biochemistry & Molecular Biology
Minjia Shen, Kelly Goldlust, Sandra Daniel, Christian Lesterlin, Yoshiharu Yamaichi
Summary: Dissemination of antibiotic resistance is driven by bacterial conjugation, which involves the transfer of single-stranded DNA from the donor to the recipient cell. Host-encoded factors, such as the uvrD gene, can affect the frequency of conjugative plasmid transfer. Our study found that disruption of the recipient uvrD gene decreased the acquisition frequency of conjugative plasmids, and that ATPase activity of UvrD is required for successful plasmid establishment in recipient cells.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Manuel Ares-Arroyo, Charles Coluzzi, Eduardo P.C. Rocha
Summary: Plasmids can be transferred between cells by conjugation, driving bacterial evolution. By studying plasmids in Escherichia coli and Staphylococcus aureus, we have solved the mystery of plasmid transfer mechanisms and revealed the functional dependencies between plasmids. These findings are important for understanding the evolutionary relationships of plasmids and the spread of antibiotic resistance genes.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Infectious Diseases
Jianxin Hu, Lin Chen, Guihua Li, Yu Pan, Yixing Lu, Jin Chen, Wenguang Xiong, Zhenling Zeng
Summary: This study investigated the epidemiology of fosB-positive Staphylococcus aureus in waterfowl farms in the Pearl River tributaries in Guangdong Province, China in 2020. The results showed that duck farm environment-derived strains contained the oxazolidinone drug resistance gene optrA, and fosB-positive S. aureus from humans and ducks could be clustered into the same clade. The study indicated that duck farms in Guangdong could be an important reservoir of fosB-positive S. aureus, and further monitoring of drug-resistant bacteria in waterfowl farms is needed.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Anna Yudkina, Nikita A. Bulgakov, Daria Kim, Svetlana Baranova, Alexander A. Ishchenko, Murat K. Saparbaev, Vladimir V. Koval, Dmitry O. Zharkov
Summary: AP sites are DNA lesions that can trap DNA-bound proteins, forming DNA-protein cross-links called APPXLs. The fate of APPXLs, however, is unclear. In this study, we synthesized two in vitro models of APPXLs using DNA glycosylases Fpg and OGG1. These APPXLs strongly blocked various DNA polymerases, but were efficiently hydrolyzed by specific AP endonucleases. Our findings suggest that the BER pathway may be responsible for removing APPXLs in bacterial and yeast cells.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Infectious Diseases
Yujie Hu, Seamus Fanning, Scott Nguyen, Wei Wang, Chang Liu, Xinnan Cui, Yinping Dong, Xin Gan, Jin Xu, Fengqin Li
Summary: CFSA629, a MDR S. Typhimurium ST34 isolate from China, carries a novel mcr-1.19 variant on an IncHI2 plasmid, highlighting the importance of surveillance to prevent the spread of mcr genes among foodborne Salmonella. Improved surveillance is crucial in controlling the dissemination of mcr genes worldwide.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
Fengzhi Liu, Ang Tian, Jingyu Wang, Yanli Zhu, Zhijing Xie, Ruihua Zhang, Shijin Jiang
Summary: This study investigated the prevalence and molecular characteristics of fosA3-harboring E. coli from ducks in Shandong province, China. The results highlighted the importance of ducks as a reservoir for multidrug-resistant fosA3-carrying E. coli.
Article
Biochemistry & Molecular Biology
Nina J. Bonde, Zachary J. Romero, Sindhu Chitteni-Pattu, Michael M. Cox
Summary: In rapidly growing cells, the pace of RecA-mediated DNA strand exchange may be too slow for bacterial DNA metabolism. However, the RadD protein, a putative SF2 family helicase, can greatly accelerate RecA-mediated DNA strand exchange, functioning only in the presence of RecA protein. This reaction requires the RadD ATPase activity, does not require an interaction with SSB, and may disassemble RecA filaments.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Beth Osia, Jerzy Twarowski, Tyler Jackson, Kirill Lobachev, Liping Liu, Anna Malkova
Summary: The study reveals that the ssDNA template formed during D-loop migration in BIR can lead to mutations. Additionally, BIR-associated ssDNA promotes replication slippage, causing genetic instability.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Adam Valcek, Petra Sismova, Kristina Nesporova, Soren Overballe-Petersen, Ibrahim Bitar, Ivana Jamborova, Arie Kant, Jaroslav Hrabak, Jaap A. Wagenaar, Jean-Yves Madec, Peter Damborg, Engeline van Duijkeren, Christa Ewers, Joost Hordijk, Henrik Hasman, Michael S. M. Brouwer, Monika Dolejska
Summary: The study found that the equine-associated Escherichia coli strains ST1250 and its variants ST1250-SLV/DLV exhibit high genetic diversity at the genomic level, with a portion of the samples carrying the epidemic multidrug resistance plasmid lineage IncHI1/ST9 and fos operon associated with E. coli-ST1250.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Katherine A. Paulin, David Cortez, Brandt F. Eichman
Summary: AP sites in DNA are common forms of DNA damage, and the replication-associated AP site repair protein HMCES plays a role in the repair of AP site-derived interstrand DNA cross-links. This study provides insights into the mechanisms of DPC formation and resolution by HMCES.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Genetics & Heredity
Sana Ahmed-Seghir, Caroline Pouvelle, Emmanuelle Despras, Agnes Cordonnier, Alain Sarasin, Patricia L. Kannouche
Article
Biochemistry & Molecular Biology
Nadege Baldeck, Regine Janel-Bintz, Jerome Wagner, Agnes Tissier, Robert P. Fuchs, Peter Burkovics, Lajos Haracska, Emmanuelle Despras, Marc Bichara, Bruno Chatton, Agnes M. Cordonnier
NUCLEIC ACIDS RESEARCH
(2015)
Article
Multidisciplinary Sciences
Emmanuelle Despras, Meghane Sittewelle, Caroline Pouvelle, Noemie Delrieu, Agnes M. Cordonnier, Patricia L. Kannouche
NATURE COMMUNICATIONS
(2016)
Article
Genetics & Heredity
Agnes Tissier, Regine Janel-Bintz, Stephane Coulon, Esther Klaile, Patricia Kannouche, Robert P. Fuchs, Agnes M. Cordonnier
Article
Biochemistry & Molecular Biology
Jerome Wagner, Helene Etienne, Robert P. Fuchs, Agnes Cordonnier, Dominique Burnouf
MOLECULAR MICROBIOLOGY
(2009)
Review
Biotechnology & Applied Microbiology
Marc Bichara, Matt Meier, Jerome Wagner, Agnes Cordonnier, Iain B. Lambert
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2011)
Article
Biochemistry & Molecular Biology
Valerie Schmutz, Regine Janel-Bintz, Jerome Wagner, Denis Biard, Naoko Shiomi, Robert P. Fuchs, Agnes M. Cordonnier
NUCLEIC ACIDS RESEARCH
(2010)
Article
Multidisciplinary Sciences
Regine Janel-Bintz, Jerome Wagner, Lajos Haracska, Marcia Chia Miao Mah-Becherel, Marc Bichara, Robert P. Fuchs, Agnes M. Cordonnier
Article
Biochemical Research Methods
Marc Vigneron, Frank Dietsch, Laurent Bianchetti, Annick Dejaegere, Yves Nomine, Agnes Cordonnier, Guy Zuber, Bruno Chatton, Mariel Donzeau
BIOCONJUGATE CHEMISTRY
(2019)
Article
Biochemical Research Methods
Regine Janel-Bintz, Lauriane Kuhn, Philippe Frit, Johana Chicher, Jerome Wagner, Lajos Haracska, Philippe Hammann, Agnes M. Cordonnier
Article
Cell Biology
Jo-Ann Nettersheim, Regine Janel-Bintz, Lauriane Kuhn, Agnes M. Cordonnier
Summary: DNA polymerase eta interacts with the calpain small subunit-1, leading to its cleavage and positively regulating its accumulation in replication foci.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemical Research Methods
Frank Dietsch, Mariel Donzeau, Agnes M. Cordonnier, Etienne Weiss, Bruno Chatton, Marc Vigneron
Article
Genetics & Heredity
Valerie Schmutz, Jerome Wagner, Regine Janel-Bintz, Robert P. P. Fuchs, Agnes M. Cordonnier
Article
Genetics & Heredity
A Tissier, P Kannouche, MP Reck, AR Lehmann, RPP Fuchs, A Cordonnier
