Article
Chemistry, Physical
Weiqing Xu, Yu Wu, Lei Jiao, Meng Sha, Xiaoli Cai, Yating Wen, Yifeng Chen, Wenling Gu, Chengzhou Zhu
Summary: Enzymes can be immobilized within metal-organic frameworks (MOFs) to protect and enhance their catalytic efficiency. This study proposes a functional protein trap strategy using ferrocenedicarboxylic acid (Fc) to induce the formation of defect-rich Co-based MOFs (CoBDC-Fc). The engineered protein trap improves enzyme loading, alters enzyme conformation, and provides cocatalysts/cofactors, resulting in excellent recyclability and tolerance to challenging conditions. The CoBDC-Fc/enzyme composites are successfully applied for cascade enzyme-linked immunosorbent assay with satisfactory sensitivity.
Article
Chemistry, Physical
Weiqing Xu, Yu Wu, Lei Jiao, Meng Sha, Xiaoli Cai, Yating Wen, Yifeng Chen, Wenling Gu, Chengzhou Zhu
Summary: Immobilizing enzymes within metal-organic frameworks (MOFs) enhances their stability in extreme environments. This study proposes a functional protein trap strategy for efficient enzyme encapsulation. Utilizing ferrocenedicarboxylic acid (Fc) induces the formation of defect-rich Co-based MOFs (CoBDC-Fc), improving enzyme loading and catalytic efficiency. The engineered trap also exhibits excellent recyclability and tolerance to inhospitable conditions, enabling its application in enzyme-linked immunosorbent assay of prostatespecific antigen with satisfactory sensitivity.
Review
Chemistry, Multidisciplinary
Wanchun Xiang, Shengzhong (Frank) Liu, Wolfgang Tress
Summary: Metal halide inorganic perovskite materials are of interest for photovoltaic applications due to their superior thermal stability. Solar cells based on inorganic perovskites have achieved a PCE over 20%, with more room for improvement compared to organic-inorganic hybrid perovskite solar cells. Interfaces play a crucial role in limiting the photovoltage and charge extraction efficiency in inorganic perovskite solar cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Satyadip Paul, Mani Gupta, Kaushik Dey, Ashok Kumar Mahato, Saikat Bag, Arun Torris, E. Bhoje Gowd, Hasnain Sajid, Matthew A. Addicoat, Supratim Datta, Rahul Banerjee
Summary: In this study, a hierarchical TpAzo COF-foam with micro-, meso-, and macroporosity was synthesized using an in situ CO2 gas effervescence technique. The resulting TpAzo foam matrix showed higher immobilization efficiency for enzymes compared to the COF. The enzyme-immobilized TpAzo foam exhibited activity and stability, and was successfully utilized for a one-pot tandem conversion reaction with high recyclability.
Review
Chemistry, Multidisciplinary
Emily Radley, John Davidson, Jake Foster, Richard Obexer, Elizabeth L. Bell, Anthony P. Green
Summary: The development and implementation of engineered enzymes and biocatalytic technologies are crucial for achieving sustainable development goals and protecting the environment. By using directed evolution and other methods, engineered enzymes can improve the sustainability of processes such as carbon capture and utilization, bioremediation, plastic deconstruction, and renewable feedstock generation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Nanoscience & Nanotechnology
Drew Jordahl, Zoe Armstrong, Qiaobin Li, Runxiang Gao, Wei Liu, Kelley Johnson, William Brown, Allison Scheiwiller, Li Feng, Angel Ugrinov, Haiyan Mao, Bingcan Chen, Mohiuddin Quadir, Hui Li, Yanxiong Pan, Zhongyu Yang
Summary: Metal-organic frameworks (MOFs) can immobilize enzymes through co-crystallization or diffusion, and biomineralization provides an effective method for enzyme immobilization under aqueous conditions. The stability of enzyme@MOF composites depends on the metal-ligand combinations and pH values. This study demonstrates the potential of expanding the library of metal-ligand selection for customized encapsulation of enzymes based on their stability, functionality, and optimal pH.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Ludwig L. Bengel, Benjamin Aberle, Alexander-N. Egler-Kemmerer, Samuel Kienzle, Bernhard Hauer, Stephan C. Hammer
Summary: Selective alkylation of pyrazoles using a catalyst-controlled enzymatic system allows for the efficient synthesis of important molecules with unprecedented regioselectivity. The use of a computational enzyme-library design tool enabled the conversion of a promiscuous enzyme into a small enzyme family of pyrazole-alkylating enzymes in one round of mutagenesis and screening. This enzymatic system demonstrates high selectivity and achieves different alkylations of pyrazoles with remarkable regiodivergence.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Miaomiao Liu, Yunhong Song, Yi-Heng P. Job Zhang, Chun You
Summary: A method for carrier-free immobilization of multi-enzyme complexes with more than four enzymes is developed using polypeptide interactions and enzyme component self-oligomerization. The self-assembled enzyme complexes show higher initial reaction rates than the four-enzyme cocktail. Water-insoluble self-assembled multi-enzyme complexes are observed, serving as carrier-free immobilized multi-enzyme complex aggregates. These immobilized enzyme complexes can be easily recycled and obtained from either purified enzyme components or crude cell extracts. This strategy sheds light on improving the catalytic capability of in vitro synthetic enzymatic biosystems.
Review
Food Science & Technology
Adriano Brandelli, Daniel Joner Daroit
Summary: Enzymatic hydrolysis is an important strategy to release bioactive peptides from food proteins. Unconventional microbial proteases (UMPs) have gained attention in recent years for their potential to produce bioactive protein hydrolysates. This review discusses the production of UMPs and their use in obtaining bioactive protein hydrolysates, with a focus on antioxidant and anti-hypertensive properties. The review also highlights the in vivo bioactivities of these hydrolysates and peptides. Further research is needed to explore the commercial utility of UMPs and the obtained hydrolysates.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Chemistry, Physical
Christopher K. Prier, Karla Camacho Soto, Jacob H. Forstater, Nadine Kuhl, Jeffrey T. Kuethe, Wai Ling Cheung-Lee, Michael J. Di Maso, Claire M. Eberle, Shane T. Grosser, Hsing- Ho, Erik Hoyt, Anne Maguire, Kevin M. Maloney, Amanda Makarewicz, Jonathan P. McMullen, Jeffrey C. Moore, Grant S. Murphy, Karthik Narsimhan, Weilan Pan, Nelo R. Rivera, Anumita Saha-Shah, David A. Thaisrivongs, Deeptak Verma, Adeya Wyatt, Daniel Zewge
Summary: Enzymes immobilized on solid supports in organic solvents have been used to catalyze a biocatalytic transaminase reaction, resulting in efficient and selective chemical synthesis. The advantages of enzyme immobilization include improved reaction efficiency, enhanced enzyme stability, reaction in non-aqueous media, and simplified product separation. By combining directed enzyme evolution with optimal reaction solvent and solid support for immobilization, the transaminase reaction of Cyrene (2) was successfully carried out, producing the amine intermediate 3a en route to the BTK inhibitor nemtabrutinib as a crystalline salt with dr > 50:1.
Review
Biochemistry & Molecular Biology
Hasan Tanvir Imam, Vladimir Krasnan, Martin Rebros, Andrew Craig Marr
Summary: Ionic liquids have unique chemical properties that have fascinated scientists in many fields. The effects of adding ionic liquids to biocatalysts are diverse, including improved separations, reduced toxicity, and stabilized protein structures. As the state of the art in ionic liquids progresses, new beneficial effects in biocatalysis are continuously being discovered.
Review
Chemistry, Physical
Zhuang Li, Qi Han, Kun Wang, Shaoyu Song, Yaju Xue, Xiuling Ji, Jiali Zhai, Yuhong Huang, Suojiang Zhang
Summary: This review provides an overview of the use of biocompatible ionic liquids (bio-ILs) in biocatalysis. It discusses the unique properties of bio-ILs and their effects on enzyme structure and activity. The review also highlights the applications of bio-ILs in homogenous and multiphasic systems, as well as their potential use in modifying enzyme carrier materials. Furthermore, it provides expert opinions on future research directions and industrial applications of bio-ILs in biocatalysis.
CATALYSIS REVIEWS-SCIENCE AND ENGINEERING
(2022)
Review
Chemistry, Multidisciplinary
Christina Jonsson, Ren Wei, Antonino Biundo, Johan Landberg, Lisa Schwarz Bour, Fabio Pezzotti, Andreea Toca, Les M. Jacques, Uwe T. Bornscheuer, Per-Olof Syren
Summary: Although the separation of individual components from polymer blends in textiles is currently unavailable at a viable scale, biotechnology may provide a solution. However, biotechnological-assisted depolymerization only works for a few synthetic polymer chains, and the use of difficult-to-recycle blended materials is rapidly growing in the textile industry.
Article
Chemistry, Inorganic & Nuclear
Jin Tan, Jie Dou, Jialong Duan, Yuanyuan Zhao, Benlin He, Qunwei Tang
Summary: Researchers introduce a trifunctional polyethylene oxide buffer layer at the interface of carbon-based all-inorganic perovskite solar cells (PSCs) to enhance the efficiency and stability of the cells. The polyethylene oxide layer increases the crystallinity of the perovskite grains, passivates defects on the perovskite surface, and improves the moisture stability of the cells. The best encapsulated PSC achieves a power conversion efficiency of 8.84% and maintains 84.8% of its initial efficiency over 30 days at 80% relative humidity.
DALTON TRANSACTIONS
(2023)
Article
Biotechnology & Applied Microbiology
Dongying Zhao, Zhongji Pu, Qiao Su, Yue Zhang, Wenhui Sun, Yongming Bao
Summary: In this study, a novel self-assembled nanoflower structure CaNF@CgkPZ was synthesized, with significantly improved catalytic efficiency and good stability and reusability.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ajith Pattammattel, Challa Vijaya Kumar
ADVANCED FUNCTIONAL MATERIALS
(2015)
Article
Biochemical Research Methods
Inoka K. Deshapriya, Bobbi S. Stromer, Ajith Pattammattel, Christina S. Kim, Ramiro Iglesias-Bartolome, Laura Gonzalez-Fajardo, Vyomesh Patel, J. Silvio Gutkind, Xiuling Lu, Challa V. Kumar
BIOCONJUGATE CHEMISTRY
(2015)
Article
Chemistry, Analytical
Ananta Ghimire, Omkar V. Zore, Vindya K. Thilakarathne, Victoria A. Briand, Patrick J. Lenehan, Yu Lei, Rajeswari M. Kasi, Challa V. Kumar
Article
Chemistry, Physical
Omkar V. Zore, Ajith Pattammattel, Shailaja Gnanaguru, Challa V. Kumar, Rajeswari M. Kasi
Article
Chemistry, Physical
Marc J. Novak, Ajith Pattammattel, Brianna Koshmerl, Megan Puglia, Christina Williams, Challa V. Kumar
Article
Chemistry, Multidisciplinary
Challa V. Kumar, Marc J. Novak, Kyle R. Benson, Clive Baveghems, Vindya K. Thilakarathne, Bobbi S. Stromer, Filicia M. Ross
Article
Chemistry, Multidisciplinary
Bobbi S. Stromer, Challa Vijaya Kumar
ADVANCED FUNCTIONAL MATERIALS
(2017)
Article
Chemistry, Multidisciplinary
C. M. Riccardi, D. Mistri, O. Hart, M. Anuganti, Y. Lin, R. M. Kasi, C. V. Kumar
CHEMICAL COMMUNICATIONS
(2016)
Article
Chemistry, Multidisciplinary
Ranjan K. Kamat, Yuting Zhang, Murali Anuganti, Wanfu Ma, Iman Noshadi, Hailin Fu, Stephen Ekatan, Richard Parnas, Changchun Wang, Challa V. Kumar, Yao Lin
Article
Chemistry, Physical
Bobbi Stromer, Melissa Limbacher, Dhanya T. Jayaram, Sudarat Yenjai, Ruma Chowdhury, Apinya Buranaprapuk, Danaboyina Ramaiah, Challa V. Kumar
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2017)
Article
Chemistry, Multidisciplinary
Ajith Pattammattel, Paritosh Pande, Deepa Kuttappan, Megan Puglia, Ashis K. Basu, Mary Anne Amalaradjou, Challa V. Kumar
Article
Chemistry, Physical
Islam M. Mosa, Ajith Pattammattel, Karteek Kadimisetty, Paritosh Pande, Maher F. El-Kady, Gregory W. Bishop, Marc Novak, Richard B. Kaner, Ashis K. Basu, Challa V. Kumar, James F. Rusling
ADVANCED ENERGY MATERIALS
(2017)
Article
Chemistry, Multidisciplinary
Omkar V. Zore, Paritosh Pande, Oghenenyerovwo Okifo, Ashis K. Basu, Rajeswari M. Kasi, Challa V. Kumar
Article
Nanoscience & Nanotechnology
Ananta Ghimire, Ajith Pattamrnattel, Charles E. Maher, Rajeswari M. Kasi, Challa V. Kumar
ACS APPLIED MATERIALS & INTERFACES
(2017)
Article
Chemistry, Multidisciplinary
Kyle Benson, Ananta Ghimire, Ajith Pattammattel, Challa V. Kumar
ADVANCED FUNCTIONAL MATERIALS
(2017)