Article
Neurosciences
Sunu Mathew, Darrell WuDunn, Devin D. Mackay, Aaron Vosmeier, Eileen F. Tallman, Rachael Deardorff, Alon Harris, Martin R. Farlow, Jared R. Brosch, Sujuan Gao, Liana G. Apostolova, Andrew J. Saykin, Shannon L. Risacher
Summary: This study investigated the association between retinal nerve fiber layer (RNFL) thickness and brain volume in older adults with subjective or objective cognitive decline. The results showed that RNFL thickness was significantly correlated with brain volumes, including global volume, temporal lobe, hippocampal, amygdala, and occipital lobe volumes. RNFL thickness may be a useful non-invasive and inexpensive tool for detection of brain neurodegeneration and may assist with diagnosis and monitoring of progression and treatment in AD.
JOURNAL OF ALZHEIMERS DISEASE
(2023)
Article
Chemistry, Physical
Anis Aliouat, Guy Antou, Nicolas Pradeilles, Vincent Rat, Alexandre Maitre
Summary: This study aims to investigate the effects of electric/electromagnetic fields generated by pulsed current in Spark Plasma Sintering (SPS) on the sintering mechanisms. The chosen model material was pre-oxidized copper composed of microsized particles. The results showed that increasing the frequency of pulsed current significantly reduces the critical time for electrical transition. Additionally, the coupling between pulsed current and mechanical stress promotes specific mechanisms in SPS.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Multidisciplinary Sciences
Maria A. Duque-Correa, David Goulding, Faye H. Rodgers, Claire Cormie, Kate Rawlinson, J. Andrew Gillis, Allison J. Bancroft, Hayley M. Bennett, Magda Lotkowska, Adam J. Reid, Anneliese Speak, Paul Scott, Nicholas Redshaw, Catherine McCarthy, Cordelia Brandt, Catherine Sharpe, Caroline Ridley, Judit Gali Moya, Claudia M. Carneiro, Tobias Starborg, Kelly S. Hayes, Nancy Holroyd, Mandy Sanders, David J. Thornton, Richard K. Grencis, Matthew Berriman
Summary: Whipworms are large parasites causing chronic disease in humans and other mammals. Researchers have discovered how the larvae invade host epithelial cells, establishing infection by degrading mucus layers and creating tunnels. This interaction between the whipworm and the host triggers an immune response and tissue repair.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Michael W. Stuck, Weng Man Chong, Jung-Chi Liao, Gregory J. Pazour
Summary: Primary cilia are essential for development and health, and dysfunction is associated with a range of human pathologies. The small GTPase Rab34 was identified as a key protein in ciliary formation, particularly important for internal assembly pathway. Additionally, it was found that IMCD3 cells can utilize the internal assembly pathway for ciliogenesis.
Article
Clinical Neurology
Georgina Hobden, Margaret J. Moore, Emma Colbourne, Sarah T. Pendlebury, Nele Demeyere
Summary: This study investigates the association between cortical atrophy and white matter lesions (WMLs) with poststroke cognitive impairment (PSCI) in specific cognitive domains. The results suggest that cortical atrophy is associated with chronic global PSCI and impairment in memory and attention domains.
Article
Agriculture, Dairy & Animal Science
Mahsa Zare, Hadi Atashi, Miel Hostens
Summary: Genomic loci associated with lactation performance during 9 to 50 days in milk in Holstein dairy cows were detected in this study. Candidate genes identified for milk production traits showed contrasting results between the early and peak stages of lactation, indicating differential genetic effects during lactation.
Article
Veterinary Sciences
Marie Louise Honore, Tina H. Pihl, Tanne M. Busk-Anderson, Laura L. Flintrup, Lise N. Nielsen
Summary: This study evaluated and compared the applicability of two human d-dimer assays in horses. The results showed that both the Stago and NycoCard assays were applicable for use in horses, but they were not directly comparable.
BMC VETERINARY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Michael Steiner, Ben Yang, Simon Hohl, Da Li, Philip Donoghue
Summary: In Mongolia, researchers have discovered a new assemblage of Cambrian eggs, embryos, and early postembryonic stages, shedding light on the development and evolution of ancient bilaterian animals.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Sarah McColman, Rui Li, Selena Osman, Amanda Bishop, Kathleen P. Wilkie, David T. Cramb
Summary: This study used Fluorescence Correlation/Cross Correlation Spectroscopy and Fluorescence Resonance Energy Transfer to directly analyze four different nanoparticle systems, finding surface heterogeneity to be a key factor in protein binding to these nanoparticles. The early hard corona is conceptualized as low-ratio, non-uniform binding rather than a uniform monolayer.
Article
Immunology
Fatima Hariss, Marie Delbeke, Karine Guyot, Pauline Zarnitzky, Mohamad Ezzedine, Gabriela Certad, Bertrand Meresse
Summary: In this study, researchers developed a co-culture model with innate IELs isolated from Rag2(-/-) mice and 3D intestinal organoids infected with C. parvum. They found that innate IELs control parasite proliferation through a perforin/granzyme-dependent mechanism. This study provides important insights into the immune response to intestinal pathogens.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Carla Lopes, I. Luisa Ferreira, Carina Maranga, Margarida Beatriz, Sandra I. Mota, Jose Sereno, Joao Castelhano, Antero Abrunhosa, Francisco Oliveira, Maura De Rosa, Michael Hayden, Mario N. Laco, Cristina Januario, Miguel Castelo Branco, A. Cristina Rego
Summary: Deficits in mitochondrial function and redox deregulation occur in early stages of Huntington's disease and can progress with disease manifestation.
Article
Hematology
Patrycja Molek, Michal Zabczyk, Krzysztof P. Malinowski, Joanna Natorska, Anetta Undas
Summary: Increased levels of circulating NETs markers associated with a hypercoagulable state may predispose anticoagulated AF patients to ischemic stroke, indicating a prognostic value of NETosis in AF.
THROMBOSIS RESEARCH
(2022)
Article
Environmental Sciences
P. Munk, B. Buongiorno Nardelli, P. Mariani, J. Bendtsen
Summary: The larvae of European eels travel a distance of approximately 5,000 km from the Sargasso Sea to the European coasts. This study focuses on the larval drift and analyzes the effects of mesoscale processes using survey data and modeling. The distribution of larvae in the Subtropical Convergence Zone was examined, and patterns in water mass distribution and particle dispersion dynamics were analyzed. The results suggest that the larval distribution is influenced by frontal zones and current shear, challenging common interpretations of eel larval drift.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Endocrinology & Metabolism
John C. Rasmussen, Melissa B. Aldrich, Caroline E. Fife, Karen L. Herbst, Eva M. Sevick-Muraca
Summary: This study investigated the lymphatic vessel anatomy and function in early stages of lipedema using near-infrared fluorescence lymphatic imaging. The results showed that while lower extremity lymphatic vessels were dilated in lipedema patients, their propulsion rates exceeded those of the control group. In contrast, upper extremity lymphatics were normal and no dermal backflow was observed in patients with Stage I and II lipedema.
Review
Multidisciplinary Sciences
Sara Barrasa-Ramos, Claire A. A. Dessalles, Mathieu Hautefeuille, Abdul I. I. Barakat
Summary: Understanding and controlling angiogenesis is crucial in fields such as oncology, cardiovascular disease, and tissue engineering. Mechanical factors play a fundamental role in angiogenesis and can be used to optimize the development of vascular networks. This review focuses on the effects of fluid and solid mechanical stimuli on the early stages of angiogenesis, and discusses the challenges in the field and potential solutions using in vitro and physics-based modeling.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Article
Biochemistry & Molecular Biology
Dmitry Gribanyov, Gleb Zhdanov, Andrei Olenin, Georgii Lisichkin, Alexandra Gambaryan, Vladimir Kukushkin, Elena Zavyalova
Summary: The development of sensitive techniques, such as Surface-enhanced Raman spectroscopy (SERS) and DNA aptamers, shows promise for combining rapid specific detection and quantitative determination of viruses, addressing the limitations of existing SERS-based aptasensors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anastasia A. Bizyaeva, Dmitry A. Bunin, Valeria L. Moiseenko, Alexandra S. Gambaryan, Sonja Balk, Vadim N. Tashlitsky, Alexander M. Arutyunyan, Alexey M. Kopylov, Elena G. Zavyalova
Summary: Nucleic acid aptamers are considered promising for specific and high-affinity binding of biomolecules. Research shows that related G-quadruplexes can bind the same protein, suggesting potential for modulating affinity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Valeria Legatova, Nadezhda Samoylenkova, Alexander Arutyunyan, Vadim Tashlitsky, Elena Zavyalova, Dmitry Usachev, Galina Pavlova, Alexey Kopylov
Summary: G-quadruplex oligonucleotides exhibit specific anti-proliferative activity in human cancer cell lines, with the novel bi-modular GQ bi-(AID-1-T) showing the highest activity against neural cancer cell lines. This could potentially lead to new treatments for glioblastoma multiform.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Rugiya Alieva, Roman Novikov, Vadim Tashlitsky, Alexander Arutyunyan, Alexey Kopylov, Elena Zavyalova
Summary: Aptamers are structured oligonucleotides that specifically bind their targets, and can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Adding aptamers can create nanostructures that bind specifically to certain targets.
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Fedor Grabovenko, Liudmila Nikiforova, Bogdan Yanenko, Andrey Ulitin, Eugene Loktyushov, Timofei Zatsepin, Elena Zavyalova, Maria Zvereva
Summary: Study on nucleic acid aptamers specific to S-protein and its receptor binding domain reveals their sensitivity to glycosylation patterns and biotinylation sites, highlighting the importance of careful testing for new viral variants in the development of specific therapy and biosensors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Elena Yu Poimanova, Polina A. Shaposhnik, Daniil S. Anisimov, Elena G. Zavyalova, Askold A. Trul, Maxim S. Skorotetcky, Oleg Borshchev, Dmitry Z. Vinnitskiy, Marina S. Polinskaya, Vadim B. Krylov, Nikolay E. Nifantiev, Elena Agina, Sergey A. Ponomarenko
Summary: EGOFETs show great potential in the field of bioelectronic sensing. This article proposes a simple and fast technique for functionalizing the semiconducting surface and demonstrates the specific response of a BTBT-based biorecognition layer to influenza A virus. The method can be used for different receptor molecules and enables the fabrication of various biosensors.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Elena Zavyalova, Daria Tikhonova, Gleb Zhdanov, Elena Rudakova, Vera Alferova, Andrey Moiseenko, Polina Kamzeeva, Alexei Khrulev, Arthur Zalevsky, Alexander Arutyunyan, Roman Novikov, Vladimir Kukushkin, Andrey Aralov
Summary: Phosphorylated adenosine derivatives play important roles in the energetic balance of the cell, biosynthesis of cell components, and regulation of protein activity. Surface-enhanced Raman spectroscopy (SERS) is an optical method that can provide a unique spectrum of a substance at low concentrations. By using nucleic acid aptamers and Raman-active dyes, the specificity and limit of detection of SERS-based sensors can be significantly increased. In this study, a biosensor based on the AMP-dependent interaction between a DNA aptamer and a novel Raman-active dye was described, showing a low limit of detection and a wide dynamic range.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Gleb Zhdanov, Ekaterina Nyhrikova, Nadezda Meshcheryakova, Olga Kristavchuk, Assel Akhmetova, Evgeny Andreev, Elena Rudakova, Alexandra Gambaryan, Igor Yaminsky, Andrey Aralov, Vladimir Kukushkin, Elena Zavyalova
Summary: Biosensors combining the ultrahigh sensitivity of surface-enhanced Raman scattering (SERS) and the specificity of nucleic acid aptamers have recently drawn attention in the detection of respiratory viruses. Here, a novel design of SERS-based aptasensor with a detection limit close to RT-PCR sensitivity is proposed. The sensor utilizes silver nanoparticles and a unique Raman-active dye to detect influenza viruses.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mariia Samodelova, Olesya O. Kapitanova, Nadezda F. Meshcheryakova, Sergey M. Novikov, Nikita R. Yarenkov, Oleg A. Streletskii, Dmitry Yakubovsky, Fedor Grabovenko, Gleb A. Zhdanov, Aleksey Arsenin, Valentyn S. Volkov, Elena G. Zavyalova, Irina A. Veselova, Maria Zvereva
Summary: The recent SARS-CoV-2 infection has presented a challenge for the development of fast virus identification methods based on sensor principles. Researchers have created a novel aptasensor using a sandwich mode with a structure modeling surface and size of the virus. The results show high stability and potential for detecting coronaviruses.
Article
Biotechnology & Applied Microbiology
Vladimir Kukushkin, Olga Kristavchuk, Evgeny Andreev, Nadezda Meshcheryakova, Olga Zaborova, Alexandra Gambaryan, Alexander Nechaev, Elena Zavyalova
Summary: Aptasensors based on surface-enhanced Raman spectroscopy (SERS) are highly desirable due to their superior specificity and low limit of detection. In this study, we developed a method that combines membrane filtration and SERS-active substrates for efficient adsorption of viruses. The specific determination of viruses was achieved using aptamers labeled with Raman-active probes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vladimir Kukushkin, Oganes Ambartsumyan, Alexei Subekin, Anna Astrakhantseva, Vladimir Gushchin, Alexandra Nikonova, Anastasia Dorofeeva, Vitaly Zverev, Anna Keshek, Nadezda Meshcheryakova, Olga Zaborova, Alexandra Gambaryan, Elena Zavyalova
Summary: In light of recurring epidemics and pandemics, rapid and reliable virus identification techniques are needed. Polymerase chain reaction with reverse transcription is a reliable and sensitive, though not rapid, tool. The antibody-based strip is a rapid, though not reliable, and sensitive tool. Alternative tools are being developed to meet all the customer's needs, such as surface-enhanced Raman spectroscopy (SERS) which allows single molecule detection in minutes. A multiplex lithographic SERS aptasensor was developed to detect several respiratory viruses in one pot within 17 min by using labeled aptamers anchored onto metal surfaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Georgii A. Pleshakov, Ivan A. Kalinin, Alexey V. Ivanov, Ilya V. Roslyakov, Igor V. Yaminsky, Kirill S. Napolskii
Summary: Platinum-based thin films are commonly used in microelectronic devices operating at temperatures higher than 500 degrees C. Adding refractory metal oxides, such as ZrO2 and HfO2, can improve the high-temperature stability of these films. This study demonstrates that a two-step annealing process for Pt-Rh/Zr multilayer films results in a more uniform film morphology and enhanced high-temperature stability compared to the commonly used one-step annealing.
Article
Chemistry, Multidisciplinary
Alexei Subekin, Rugiya Alieva, Vladimir Kukushkin, Ilya Oleynikov, Elena Zavyalova
Summary: Surface-enhanced Raman scattering (SERS) is a powerful technique for the detection of analytes. In this paper, a SERS-based sensor was proposed for the rapid and sensitive detection of botulinum toxin type A using a functionalized silver nanoisland substrate. The proposed sensor architecture allows for one-stage detection without additional staining, with a limit of detection of 2.4 ng/mL.
Article
Chemistry, Analytical
Elena Y. Poimanova, Elena G. Zavyalova, Elena A. Kretova, Anton A. Abramov, Askold A. Trul, Oleg V. Borshchev, Anna K. Keshek, Sergey A. Ponomarenko, Elena V. Agina
Summary: In this paper, the possibility of fast quantitative specific determination of virus particles using EGOFET-based aptasensors in an aqueous environment was demonstrated. The sensitivity and selectivity of the devices were examined using various analytes including influenza A and B viruses, Newcastle disease viruses, and allantoic fluid. The effects of the thickness of the semiconducting layer on the sensory properties of EGOFETs were discussed, and the fabrication of a multi-flow cell and a multi-sensor flow device were reported. The responses obtained with the portable multi-flow mode were well correlated with those obtained in the laboratory stationary mode.
Meeting Abstract
Biochemistry & Molecular Biology
A. Bizyaeva, V. Moiseenko, D. Bunin, A. Gambaryan, A. Arutyunyan, V. Tashlitsky, A. Kopylov, E. Zavyalova
