Review
Food Science & Technology
Guoyong Jiang, Yu Li, Jinghan Liu, Ling Liu, Fuwei Pi
Summary: Aptamer sensors, based on molecular interactions, have been extensively studied for their ability to specifically identify food contaminants. The integration of surface-enhanced Raman spectroscopy with aptamers offers advantages in sensitivity and speed. These sensors have applications in detecting biological and chemical contamination in food.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Nanoscience & Nanotechnology
Napatsakorn Kamkrua, Thitaphat Ngernsutivorakul, Saksorn Limwichean, Pitak Eiamchai, Chanonthorn Chananonnawathorn, Viyapol Pattanasetthakul, Raffaele Ricco, Kiattawee Choowongkomon, Mati Horprathum, Noppadon Nuntawong, Tanujjal Bora, Raju Botta
Summary: In this study, a SERS-based aptasensor platform was developed for the sensitive and selective detection of the herbicide paraquat (PQ) by modifying gold (Au) nanoparticle SERS substrates with a thiol-containing aptamer. The aptamer-modified SERS substrates showed improved sensitivity and selective detection of PQ, with a limit of detection estimated to be 0.10 ± 0.03 μM. The developed aptasensor platform demonstrated good stability and selectivity against interferences from other similar herbicides and insecticides in natural water samples.
ACS APPLIED NANO MATERIALS
(2023)
Article
Engineering, Environmental
Ting Wang, Shuangpeng Wang, Zehua Cheng, Jinchao Wei, Lele Yang, Zhangfeng Zhong, Hao Hu, Yitao Wang, Bingpu Zhou, Peng Li
Summary: Pesticide residues pose a significant threat to human health and the environment, driving the need for rapid and effective detection methods. Core-shell nanostructures as novel SERS substrates have been widely utilized in rapid pesticide detection research. Understanding and monitoring pesticide residues can be improved through the classification and evaluation of different core-shell nanostructures and early-warning analysis.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemical Research Methods
Yanyan Zhang, Linze Li, Hao Zhang, Junjian Shang, Can Li, Syed Muhammad Zaigham Abbas Naqvi, Zephania Birech, Jiandong Hu
Summary: An aptamer sensor based on surface-enhanced Raman spectroscopy (SERS) was developed for the high sensitivity detection of abscisic acid (ABA). The sensor showed a low detection limit and a good linear response. In practical application, the sensor demonstrated a low relative error compared to the enzyme-linked immunosorbent assay (ELISA) in detecting ABA from fresh wheat leaves.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Zhiyang Pei, Jing Li, Chang Ji, Jibing Tan, Zhida Shao, Xiaofei Zhao, Zhen Li, Baoyuan Man, Jing Yu, Chao Zhang
Summary: We have proposed and prepared a cascaded wire-in-cavity-in-bowl (WICIB) structure on flexible polydimethysiloxane to overcome the sensitivity to excitation angles in surface-enhanced Raman scattering (SERS) sensors and enable monitoring of contaminants in complex environments. The WICIB structure serves as a highly sensitive, homogeneous, and stable SERS substrate for conventional detection. Through finite element simulations, it was proven that plasmonic coupling and distribution of the enhanced electromagnetic field occur around the wire and inside the cavity, making it beneficial for polydirectional and in situ detection. By exploiting the triple synergistic enhancement effect and unique optical properties, we successfully achieved the in situ detection of residual pollutant molecules in microdroplets of apple juice and lake water, indicating the great potential of this flexible SERS sensor for on-site environmental monitoring.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Gleb Zhdanov, Ekaterina Nyhrikova, Nadezda Meshcheryakova, Olga Kristavchuk, Assel Akhmetova, Evgeny Andreev, Elena Rudakova, Alexandra Gambaryan, Igor Yaminsky, Andrey Aralov, Vladimir Kukushkin, Elena Zavyalova
Summary: Biosensors combining the ultrahigh sensitivity of surface-enhanced Raman scattering (SERS) and the specificity of nucleic acid aptamers have recently drawn attention in the detection of respiratory viruses. Here, a novel design of SERS-based aptasensor with a detection limit close to RT-PCR sensitivity is proposed. The sensor utilizes silver nanoparticles and a unique Raman-active dye to detect influenza viruses.
FRONTIERS IN CHEMISTRY
(2022)
Article
Instruments & Instrumentation
Rui Li, Lijun Li, Yan Zhang, Xin Lin, Heyuanxi Guo, Chubing Lin, Jun Feng
Summary: In this paper, a SERS aptasensor for CEA detection was developed using a silver nanorod array chip, thiol aptamer, and 4-mercaptophenylboronic acid. The sensor showed good specificity, high sensitivity, and a wide linear range, allowing for the detection of CEA in serum.
APPLIED SPECTROSCOPY
(2023)
Article
Spectroscopy
Jie Zhou, Dan Wang, Haifeng Yang, Feng Wang
Summary: Acetamiprid poses a serious threat to human beings. This study presents a rapid and sensitive detection method for acetamiprid using highly transparent PCR-M membranes and acetamiprid aptamer. Direct and indirect detection methods were employed to achieve different detection limits for acetamiprid and its CN group.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Applied
Baihui Wang, Huan Jiang, Ruyi Tang, Yaoyu Tan, Xuemin Xia, Xia Zhang
Summary: This paper presents the development of a novel aptamer sensor for rapid, sensitive, and specific detection of histamine. The sensor design is based on the adsorption of histamine aptamers onto the surface of Au NPs through bioconjugation, promoting the peroxidase activity of the nanozymes. The sensor shows a wide linear range and high accuracy, making it a promising platform for detecting various molecules by combining aptamers with Au NPs.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Spectroscopy
Lei Jin, Shufang Wang, Qing Shao, Yiyu Cheng
Summary: This study presents an aptamer-based surface enhanced Raman spectroscopy (SERS) method for rapid detection of Salmonella Enteritidis. The method utilizes a handheld Raman instrument and shows high specificity, quick detection time, and quantitative determination of Salmonella Enteritidis. It offers a promising alternative for on-site detection of Salmonella Enteritidis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Shuxin Chen, Xiaowen Lv, Jifan Shen, Siqi Pan, Zhiliang Jiang, Yang Xiao, Guiqing Wen
Summary: A new method for the determination of oxytetracycline (OTC) has been established by coupling the catalytic amplification reaction of copper nanoclusters (CuNCs) with the aptamer reaction. The experimental results showed that OTC could inhibit the catalytic activity of CuNCs in a non-specific way, and a specific reaction could restore the catalytic activity for OTC detection. The study demonstrated a linear relationship between the intensities of SERS or RRS signals and OTC concentration, providing a novel approach for trace OTC determination.
Article
Spectroscopy
Junlin Chen, Hong Lin, Limin Cao, Jianxin Sui, Lei Wang, Xiu Fang, Kaiqiang Wang
Summary: A portable surface-enhanced Raman scattering (SERS) aptasensor was developed for rapid on-site detection of chloramphenicol (CAP) residues in fish. The aptasensor utilized Fe3O4@Au nanoflowers as capture probes and Au@Ag NPs embedded with 4-mercaptobenzoic acid (4-MBA) as reporter probes. The SERS aptasensor showed excellent performance for CAP detection in fish samples, offering a promising portable analytical tool for drug residue analysis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biotechnology & Applied Microbiology
Vladimir Kukushkin, Olga Kristavchuk, Evgeny Andreev, Nadezda Meshcheryakova, Olga Zaborova, Alexandra Gambaryan, Alexander Nechaev, Elena Zavyalova
Summary: Aptasensors based on surface-enhanced Raman spectroscopy (SERS) are highly desirable due to their superior specificity and low limit of detection. In this study, we developed a method that combines membrane filtration and SERS-active substrates for efficient adsorption of viruses. The specific determination of viruses was achieved using aptamers labeled with Raman-active probes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Xueyan Chen, Qianqian Ding, Chao Bi, Jian Ruan, Shikuan Yang
Summary: This study reports on the use of an acoustic levitation platform for lossless concentration of trace molecules, enabling analyte detection in liquids, solid objects, and the gas phase. The platform allows for the concentration of analytes dissolved in volatile liquids and the simultaneous concentration of gold nanoparticles, enabling sensitive surface-enhanced Raman scattering detection even at extremely low concentrations.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Chongning Li, Xue He, Hui Li, Yang Xiao, Xiaona Xu, Caina Jiang, Guiqing Wen, Zhiliang Jiang
Summary: A new covalent organic framework loaded gold nanocluster (AuCOF) with strong catalytic activity was prepared, which can catalyze the reduction of HAuCl4 to form gold nanoparticles (AuNP) with surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) effects. The aptamer of estradiol (E2) can adsorb on the AuCOF surface to suppress its catalytic performance, and desorb from the surface to recover the catalysis upon selective binding to E2.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Iuliia Pavlova, Nikolay Barinov, Roman Novikov, Vjacheslav Severov, Mikhail Iudin, Tatiana Vedekhina, Andrey Larin, Vladislav Babenko, Andrey Aralov, Evgeny Gnuchikh, Makar Sardushkin, Dmitry Klinov, Vladimir Tsvetkov, Anna Varizhuk
Summary: G-quadruplexes (G4s) are potential regulators of chromatin packaging, and a new in vitro model was designed to study their impact on nucleosome occupancy. Stable G4 structures were found to decrease nucleosome formation, supporting the negative correlation between stable G4s and nucleosome density. This study emphasizes the importance of considering chromatin context when targeting genomic G4s.
Article
Biochemistry & Molecular Biology
Dmitry A. Veryutin, Irina A. Doroshenko, Ekaterina A. Martynova, Ksenia A. Sapozhnikova, Elena V. Svirshchevskaya, Anna V. Shibaeva, Alina A. Markova, Alexey A. Chistov, Natalya E. Borisova, Maxim V. Shuvalov, Vladimir A. Korshun, Vera A. Alferova, Tatyana A. Podrugina
Summary: Heptamethine carbocyanine dyes with bright fluorescence in the near-IR range and affinity to cancer cells can be used as fluorescent labels and vectors for drug delivery. This study synthesized drug-dye dyads and evaluated their properties, structure-activity relationship, and scope. The results suggest that the structure of the cyanine-anthracycline conjugate is crucial for its biological activity, indicating the potential of expanding the chemical space for diagnostic and therapeutic applications.
Article
Biochemistry & Molecular Biology
Vera A. Alferova, Tinashe P. Maviza, Mikhail V. Biryukov, Yuliya V. Zakalyukina, Vladimir I. Polshakov, Petr V. Sergiev, Vladimir A. Korshun, Ilya A. Osterman
Summary: Recently discovered aromatic polyketide tetracenomycins are potent inhibitors of protein synthesis, with a unique binding site located within the tunnel of the large ribosomal subunit. We report the isolation and structure elucidation of a novel tetracenomycin congener, O4-Me-tetracenomycin C, which is isomeric to tetracenomycin X. However, unlike tetracenomycin X, O4-Me-tetracenomycin C showed no antimicrobial activity and failed to inhibit protein synthesis in vitro. Structural alignment of tetracenomycins revealed the crucial role of the 4-hydroxyl group. These findings are important for the development of semi-synthetic tetracenomycins as potential antibacterials.
Article
Pharmacology & Pharmacy
Alexey A. Chistov, Stepan P. Chumakov, Igor E. Mikhnovets, Timofei D. Nikitin, Nikita A. Slesarchuk, Victoria I. Uvarova, Anna A. Rubekina, Yulia V. Nikolaeva, Eugene V. Radchenko, Evgeny V. Khvatov, Alexey A. Orlov, Vasilisa S. Frolenko, Maksim Sukhorukov, Ekaterina S. Kolpakova, Elena Y. Shustova, Anastasiya Galochkina, Philipp P. Streshnev, Eugene M. Osipov, Ksenia A. Sapozhnikova, Andrey V. Moiseenko, Vladimir A. Brylev, Gleb V. Proskurin, Yuri S. Dokukin, Sergey V. Kutyakov, Andrey V. Aralov, Vladimir A. Korshun, Sergei V. Strelkov, Vladimir A. Palyulin, Aydar A. Ishmukhametov, Evgeny A. Shirshin, Dmitry I. Osolodkin, Anna A. Shtro, Liubov I. Kozlovskaya, Vera A. Alferova, Alexey V. Ustinov
Summary: A new series of compounds based on a 5-(perylen-3-ylethynyl)-uracil scaffold showed potent broad-spectrum antiviral activity against arthropod-borne viruses (TBEV, YFV, and CHIKV) as well as respiratory viruses (SARS-CoV-2 and IAV). The compounds inhibited viral fusion and caused damage to the viral envelope, suggesting a photoinduced ROS-mediated mode of antiviral action.
ANTIVIRAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Kseniya A. A. Mariewskaya, Maxim S. S. Krasilnikov, Vladimir A. A. Korshun, Alexey V. V. Ustinov, Vera A. A. Alferova
Summary: Many organic photosensitizers can generate reactive oxygen species under visible light irradiation, showing broad antiviral activity in vitro. However, the low tissue penetration of visible light hinders the further development of these compounds as antiviral therapeutics. One possible solution is the development of near-infrared absorbing photosensitizers. This study aims to evaluate the therapeutic prospects of various NIR-absorbing and singlet oxygen-generating chromophores for the development of broad-spectrum photosensitizing antivirals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Vladimir Kukushkin, Olga Kristavchuk, Evgeny Andreev, Nadezda Meshcheryakova, Olga Zaborova, Alexandra Gambaryan, Alexander Nechaev, Elena Zavyalova
Summary: Aptasensors based on surface-enhanced Raman spectroscopy (SERS) are highly desirable due to their superior specificity and low limit of detection. In this study, we developed a method that combines membrane filtration and SERS-active substrates for efficient adsorption of viruses. The specific determination of viruses was achieved using aptamers labeled with Raman-active probes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ksenia A. Sapozhnikova, Evgeny L. Gulyak, Vladimir A. Brylev, Vsevolod A. Misyurin, Sergey D. Oreshkov, Anastasiya V. Alexeeva, Dmitry Yu. Ryazantsev, Maria A. Simonova, Ekaterina V. Ryabukhina, Galina P. Popova, Nataliya A. Tikhonova, Natalia A. Lyzhko, Alexander E. Barmashov, Andrey V. Misyurin, Alexey V. Ustinov, Vera A. Alferova, Vladimir A. Korshun
Summary: A universal approach to construct antibody-drug conjugates (ADCs) has been developed by oxidizing glycans and utilizing ligation and cycloaddition reactions for conjugation. This method allows for the synthesis of cytotoxic conjugates targeting tumor-associated antigens. The conjugates showed varying cytotoxicity in vitro, with one specific conjugate demonstrating activity against PRAME-expressing cancer cell lines, making it the first reported PRAME-targeting ADC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Polina Kamzeeva, Ivan Petushkov, Ekaterina Knizhnik, Robert Snoeck, Yuri Khodarovich, Ekaterina Ryabukhina, Vera Alferova, Artur Eshtukov-Shcheglov, Evgeny Belyaev, Julia Svetlova, Tatiana Vedekhina, Andrey Kulbachinskiy, Anna Varizhuk, Graciela Andrei, Andrey Aralov
Summary: Emerging and re-emerging viruses cause global outbreaks and epidemics, highlighting the need for new antiviral drugs. Researchers synthesized and evaluated nucleoside analogs and their derivatives, finding that one compound had antiviral activity against coronaviruses and specifically inhibited biocondensate formation important for replication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kseniya A. Mariewskaya, Daniil A. Gvozdev, Alexey A. Chistov, Petra Strakova, Ivana Huvarova, Pavel Svoboda, Jan Kotoucek, Nikita M. Ivanov, Maxim S. Krasilnikov, Mikhail Y. Zhitlov, Alexandra M. Pak, Igor E. Mikhnovets, Timofei D. Nikitin, Vladimir A. Korshun, Vera A. Alferova, Josef Masek, Daniel Rfzek, Ludek Eyer, Alexey V. Ustinov
Summary: Novel perylenylethynylphenols show promising antiviral activity against SARS-CoV-2 and FIPV by blocking viral entry and generating singlet oxygen under visible light. These compounds possess a unique combination of shape and singlet oxygen production, making them effective against enveloped viruses.
Article
Chemistry, Analytical
Elena Y. Poimanova, Elena G. Zavyalova, Elena A. Kretova, Anton A. Abramov, Askold A. Trul, Oleg V. Borshchev, Anna K. Keshek, Sergey A. Ponomarenko, Elena V. Agina
Summary: In this paper, the possibility of fast quantitative specific determination of virus particles using EGOFET-based aptasensors in an aqueous environment was demonstrated. The sensitivity and selectivity of the devices were examined using various analytes including influenza A and B viruses, Newcastle disease viruses, and allantoic fluid. The effects of the thickness of the semiconducting layer on the sensory properties of EGOFETs were discussed, and the fabrication of a multi-flow cell and a multi-sensor flow device were reported. The responses obtained with the portable multi-flow mode were well correlated with those obtained in the laboratory stationary mode.
Review
Biochemistry & Molecular Biology
Polina N. Kamzeeva, Andrey V. Aralov, Vera A. Alferova, Vladimir A. Korshun
Summary: The emergence of new viruses and drug-resistant strains has accelerated the search for new drugs, and nucleoside analogues (NAs) are a promising class of antivirals due to their known safety profiles for rapid repurposing against emerging pathogens. Recent improvements in research methods have revealed new details in the mechanisms of action of NAs, paving the way for the development of more effective drugs. This review discusses advanced techniques in viral polymerase targeting, new viral and host enzyme targeting approaches, and prodrug-based strategies for the development of antiviral NAs.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Igor E. Mikhnovets, Jiri Holoubek, Irina S. Panina, Jan Kotoucek, Daniil A. Gvozdev, Stepan P. Chumakov, Maxim S. Krasilnikov, Mikhail Y. Zhitlov, Evgeny L. Gulyak, Alexey A. Chistov, Timofei D. Nikitin, Vladimir A. Korshun, Roman G. Efremov, Vera A. Alferova, Daniel Ruzek, Ludek Eyer, Alexey V. Ustinov
Summary: Amphipathic perylene derivatives are effective antiviral compounds that target the viral envelope and act as fusion inhibitors. They damage the viral envelope using lipophilic perylene and singlet oxygen generated by light. Short alkyl chains contribute to strong antiviral activity, while longer alkyl chains reduce activity due to increased lipophilicity and aggregation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Polina Kamzeeva, Nikolai Dagaev, Sofia Lizunova, Yuri Khodarovich, Anna Sogomonyan, Anastasia Kolchanova, Vadim Pokrovsky, Vera Alferova, Alexey Chistov, Artur Eshtukov-Shcheglov, Elizaveta Eshtukova-Shcheglova, Evgeny Belyaev, Dmitry Skvortsov, Anna Varizhuk, Andrey Aralov
Summary: Azacarbazoles and its derivatives have significant anti-pathogenic and antitumor activities. This study focused on the synthesis and evaluation of a series of structurally related tetracyclic compounds. The lead derivative, 5b, showed promising anti-proliferative activity against lung adenocarcinoma cells and selectivity towards certain fibroblasts. Further experiments indicated that intercalation might be the mechanism underlying its activity, rather than the modulation of G4-regulated oncogene expression. Additionally, a water-soluble salt form of 5b exhibited no acute toxicity or behavioral changes in mice.
Article
Biochemistry & Molecular Biology
Ekaterina Knizhnik, Stepan Chumakov, Julia Svetlova, Iulia Pavlova, Yuri Khodarovich, Vladimir Brylev, Vjacheslav Severov, Rugiya Alieva, Liubov Kozlovskaya, Dmitry Andreev, Andrey Aralov, Anna Varizhuk
Summary: Ribosomal frameshifting at the slippery site of SARS-CoV-2 RNA is crucial for viral replication, and inhibition of this process by oligonucleotide-based antivirals shows promise. In this study, locked nucleic acids (LNA) and other oligonucleotide analogs targeting the pseudoknot near the slippery site were shown to induce PK unfolding and inhibit frameshifting in a dose-dependent manner. These analogs also demonstrated the ability to reduce SARS-CoV-2 replication at subtoxic concentrations in human cell lines, highlighting their potential as antiviral agents.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)