Article
Multidisciplinary Sciences
Michael S. Hwang, Brian J. Mog, Jacqueline Douglass, Alexander H. Pearlman, Emily Han-Chung Hsiue, Suman Paul, Sarah R. DiNapoli, Maximilian F. Konig, Drew M. Pardoll, Sandra B. Gabelli, Chetan Bettegowda, Nickolas Papadopoulos, Bert Vogelstein, Shibin Zhou, Kenneth W. Kinzler
Summary: Targeting cancer cells with engineered T cells using NOT-gate Boolean logic to counterexpress antigens resulting from LOH events shows promise in selectively killing cancer cells, suggesting a potential therapeutic approach for diverse cancers.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Chemistry, Multidisciplinary
Fei Wang, Aftab Ullah, Xuelian Fan, Zhou Xu, Rongling Zong, Xuewen Wang, Gang Chen
Summary: An appropriate delivery system can enhance the immune effects of antigens against infections or tumors. Targeting vaccine delivery systems to APCs is a promising strategy for provoking potent immune responses.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biotechnology & Applied Microbiology
Sangwook Oh, Xuming Mao, Silvio Manfredo-Vieira, Jinmin Lee, Darshil Patel, Eun Jung Choi, Andrea Alvarado, Ebony Cottman-Thomas, Damian Maseda, Patricia Y. Y. Tsao, Christoph T. T. Ellebrecht, Sami L. L. Khella, David P. P. Richman, Kevin C. C. O'Connor, Uri Herzberg, Gwendolyn K. K. Binder, Michael C. C. Milone, Samik Basu, Aimee S. S. Payne
Summary: Engineered T cells expressing MuSK-CAART effectively target B cells expressing anti-MuSK autoantibodies, reducing muscle weakness caused by autoimmune disease.
NATURE BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Nicolas Tsapis, Franceline Reynaud, Didier Desmaele, Laurence Moine, Juliette Vergnaud, Sonia Abreu, Pierre Chaminade, Elias Fattal, Ludmila Pinheiro do Nascimento
Summary: A nanoprodrug of budesonide palmitate decorated with mannose moieties was designed to improve macrophage targeting of glucocorticoids for anti-inflammatory therapy. The nanoprodrug showed successful synthesis and encapsulation with high loading efficiency. It demonstrated significantly greater internalization by macrophages and exhibited a mannose receptor-mediated uptake. After inflammation induction, the nanoprodrug showed effective anti-inflammatory effects.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Review
Immunology
Edgar Alonso Melgoza-Gonzalez, Lorena Bustamante-Cordova, Jesus Hernandez
Summary: Advances in antigen targeting have shown promise as an alternative approach for challenging diseases in veterinary medicine. The success of antigen targeting relies on the chosen receptor, which influences the immune response. Research has explored various methods such as antibodies, ligands, fused proteins, and DNA vaccines in different veterinary species. This review highlights the recent advances in antigen targeting in pigs, cattle, sheep, poultry, and dogs in order to benefit animal health.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Polymer Science
Haibo Feng, Xiaonong Yang, Linzi Zhang, Qianqian Liu, Yangyang Feng, Daiyan Wu, Yunjie Liu, Jie Yang
Summary: The MAN-CS-OVA-PLGA-MPs delivery system shows potential for enhancing immune responses by activating T cells, promoting dendritic cell maturation, and improving antigen presentation efficiency. This targeting system not only boosts DC activity, but also significantly impacts cytokine production and specific antibody levels in mice.
Review
Chemistry, Multidisciplinary
Piotr Szpakowski, Dominika Ksiazek-Winiarek, Andrzej Glabinski
Summary: Multiple sclerosis is a common neurological disease affecting young adults, with the immune system playing a major role in its development. Current treatments targeting immunocompetent cells have shown effectiveness in managing the disease. Antigen-presenting cells play a crucial role in the pathogenesis of MS, and potential therapeutic options targeting these cells are being explored.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Corentin Gauthier, Julie Mariot, Morgane Daurat, Christine Dhommee, Khaled El Cheikh, Elodie Morere, Geoffrey Depaepe, Magali Gary-Bobo, Alain Morere, Marcel Garcia, Ilaria Basile, Valerie Gouilleux-Gruart, Marie Maynadier
Summary: The grafting of AMFA on therapeutic proteins improves enzyme delivery by increasing cellular uptake via the M6PR receptor. In this study, the impact of AMFA grafting on the cell uptake and recycling of a monoclonal antibody was investigated. AMFA grafting increased drug release in cells and had no effect on antibody recycling mediated by the FcRn receptor. AMFA grafting did not impair the pharmacokinetics and showed high stability.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Anqi Wang, Kai Yue, Weishen Zhong, Genpei Zhang, Lei Wang, Hao Wang, Hua Zhang, Xinxin Zhang
Summary: As nanotechnology is applied in clinical medicine, nanoparticle-based therapy targeting atherosclerosis through ligand-receptor interaction shows promising results. In this study, a biomimetic peptide specifically targeting the lyso-phosphatidylcholine (LPC) receptor in atherosclerotic plaques was developed and its interaction with LPC was investigated. The peptide showed strong binding capacity with LPC and a decrease in interaction forces with oxidized low-density lipoprotein (oxLDL) under acidic environments, but still remained effective for atherosclerosis treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Hitoshi Maeda, Yu Ishima, Junji Saruwatari, Yuki Mizuta, Yuki Minayoshi, Shota Ichimizu, Hiroki Yanagisawa, Taisei Nagasaki, Kengo Yasuda, Shun Oshiro, Manabu Taura, Matthew J. McConnell, Kentaro Oniki, Kayoko Sonoda, Tomohiko Wakayama, Manabu Kinoshita, Tsuyoshi Shuto, Hirofumi Kai, Motohiko Tanaka, Yutaka Sasaki, Yasuko Iwakiri, Masaki Otagiri, Hiroshi Watanabe, Toru Maruyama
Summary: The study developed a nano-antioxidant targeting Kupffer cells and combined it with a nitric oxide donor to improve therapeutic effects on NASH. Results suggest that this nano-therapy has potential for treating NASH.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Medicine, Research & Experimental
Haihong Zhao, Lina Wu, Jiemin Dai, Ke Sun, Zhenguo Zi, Junhua Guan, Liwen Zhang
Summary: In this study, a novel adoptive T-cell therapy targeting ovarian cancer was developed. By employing two binding motifs simultaneously to target CA125, the therapy showed enhanced killing effect and prolonged control of tumor growth, leading to improved overall survival.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Jinrong Yang, Weilin Zhou, Dan Li, Ting Niu, Wei Wang
Summary: This article summarizes the application and research progress of BCMA-targeting CAR T cell therapy in the treatment of multiple myeloma, as well as measures to improve efficacy and safety.
Article
Oncology
Jatuporn Sujjitjoon, Elias Sayour, Shih-Ting Tsao, Mongkol Uiprasertkul, Kleebsabai Sanpakit, Jassada Buaboonnam, Pa-thai Yenchitsomanus, La-ongsri Atchaneeyasakul, Lung-Ji Chang
Summary: GD2-specific CAR T cells showed promising therapeutic potential against retinoblastoma by recognizing and effectively killing cells with high GD2 antigen expression in vitro. However, prolonged exposure to the tumor resulted in a diminished killing activity of GD2-CAR T cells, suggesting the potential benefit of combination therapy with immune checkpoint inhibitors.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Immunology
Rui Zheng, Yuankun Chen, Yiting Zhang, Sixin Liang, Xiaojuan Zhao, Yiyi Wang, Pengju Wang, Ruotong Meng, Angang Yang, Bo Yan
Summary: Our study explores the effect of low-affinity CARs using humanized scFvs on the function of CAR-T cells. We find that moderately reducing the affinity of CARs can maintain anti-tumor efficacy and improve the safety of CAR therapy both in vitro and in vivo. In addition, T cells expressing the VL domain only antibody show long-lasting tumor elimination capability and lower cytokine levels.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Biomedical
Chuanxi Zhang, Yulu Miao, Yinghui Feng, Jiawei Wang, Zhuoli Tian, Juan Dong, Bei Gao, Lujia Zhang
Summary: This study establishes a system to detect umami peptides targeting human T1R1 receptors and investigates the molecular mechanisms involved. The results show that different peptide binding sites and peptide hydrophobicity play important roles in determining taste thresholds and affinity. This research enables rapid screening of high-intensity umami peptides and the development of T1R1 receptor-based umami detection sensors.
Article
Chemistry, Analytical
Andreas Frutiger, Christof Fattinger, Janos Voros
Summary: This paper discusses the principles and characteristics of label-free optical biosensors, as well as the impact of stabilization conditions on their performance. It introduces the concept of spatial affinity lock-in as a universal design principle for sensitive molecular sensors. The use of signal processing tools to overcome the cross-sensitivity of these sensors is also highlighted.
Article
Chemistry, Analytical
Andreas Frutiger, Karl Gatterdam, Yves Blickenstorfer, Andreas Michael Reichmuth, Christof Fattinger, Janos Voros
Summary: Advancements in sensor technology have led to the development of new label-free optical biosensors that may replace traditional refractometric biosensors, achieving higher resolution and sensitivity. This could enhance sensor applications in environmental and medical fields, and potentially bring olfactory and gustatory senses to smart devices.
Article
Engineering, Biomedical
Byron Llerena Zambrano, Aline F. Renz, Tobias Ruff, Samuel Lienemann, Klas Tybrandt, Janos Voros, Jaehong Lee
Summary: This article systematically describes the recent progress of implantable soft electronics based on various conductive nanocomposites, focusing on representative fabrication approaches and various in vivo applications. The mechanical mismatch between implanted devices and biological environments can induce damages in the body, especially for long-term applications, which stretchable electronics effectively improve.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Analytical
Nako Nakatsuka, Alix Failletaz, Dominic Eggemann, Csaba Forro, Janos Voros, Dmitry Momotenko
Summary: Artificial quartz nanopipettes functionalized with aptamers are able to reversibly recognize serotonin with high specificity and can detect physiologically relevant amounts of serotonin in complex environments. By utilizing complementary techniques such as quartz crystal microbalance and electrochemical impedance spectroscopy, the detection mechanism and theoretical model for aptamer-modified nanopipettes are investigated, demonstrating their potential as versatile tools for biological systems.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Andreas M. Reichmuth, Katharina Kuebrich, Yves Blickenstorfer, Andreas Frutiger, Dmitry Momotenko, Volker Gatterdam, Fridolin Treindl, Christof Fattinger, Janos Voeroes
Summary: A new diffractometric assay method was demonstrated in the study, which can investigate complex samples like blood plasma without labeling, and the feasibility of real-time molecular profiling of blood plasma was achieved using low-affinity binders.
Article
Biochemistry & Molecular Biology
Nako Nakatsuka, Kelly J. Heard, Alix Failletaz, Dmitry Momotenko, Janos Voros, Fred H. Gage, Krishna C. Vadodaria
Summary: This study successfully detected secreted 5-HT from human serotonergic neurons in vitro using innovative nanotechnology, with the capability of detecting very low concentrations of 5-HT. This method provides a new tool for studying neurotransmitters and offers the potential for fast quantification of neurotransmitters secreted from patient-derived neuronal cells.
MOLECULAR PSYCHIATRY
(2021)
Article
Chemistry, Multidisciplinary
Ilaria Incaviglia, Andreas Frutiger, Yves Blickenstorfer, Fridolin Treindl, Giulia Ammirati, Ines Luechtefeld, Birgit Dreier, Andreas Plueckthun, Janos Voeroes, Andreas M. Reichmuth
Summary: Cell-based assays are frequently used for molecular interaction analysis, offering specific and real-time insights into interactions involving native membrane receptors in living cells. The introduction of cell-based molography has expanded the range of interactions that can be analyzed by biosensors, now including cytosolic protein-protein interactions. This approach overcomes the limitations of label-based assays, providing a novel method for studying specific molecular interactions in living cells.
Article
Engineering, Electrical & Electronic
Jaehong Lee, Stephan J. Ihle, Guglielmo Salvatore Pellegrino, Hwajoong Kim, Junwoo Yea, Chang-Yeop Jeon, Hee-Chang Son, Chaewon Jin, Daniel Eberli, Florian Schmid, Byron Llerena Zambrano, Aline F. Renz, Csaba Forro, Hongsoo Choi, Kyung-In Jang, Roland Kung, Janos Voros
Summary: This paper proposes a capacitive nanofiber sensor for biomedical strain-sensing system, which can achieve wireless readout by combining with an inductive coil, and is of great significance for solving the problem of monitoring biomechanical strain in clinical practice.
NATURE ELECTRONICS
(2021)
Review
Chemistry, Multidisciplinary
Andreas Frutiger, Alexander Tanno, Stephanie Hwu, Raphael F. Tiefenauer, Janos Voros, Nako Nakatsuka
Summary: The differentiation between specific and nonspecific binding in molecular interactions has been achieved in nature through precise control of biomolecules. However, artificial biosensors have struggled to overcome this issue. Through theoretical models and classification frameworks, it is possible to gain a better understanding and optimize the performance of biosensors, while exploring ways to address the challenge of nonspecific binding.
Article
Chemistry, Multidisciplinary
Byron Llerena Zambrano, Csaba Forro, Erik Poloni, Robert Hennig, Pragash Sivananthaguru, Aline F. Renz, Andre R. Studart, Janos Voeroes
Summary: This research demonstrates the manipulation of nanowires using superparamagnetic iron oxide nanoparticles and vacuum filtration, resulting in different structures of oriented nanowires. The study also investigates the strain-dependent electrical properties of nanowires within composite materials.
Article
Neurosciences
Jens Duru, Joeel Kuechler, Stephan J. Ihle, Csaba Forro, Aeneas Bernardi, Sophie Girardin, Julian Hengsteler, Stephen Wheeler, Janos Voeroes, Tobias Ruff
Summary: Bottom-up neuroscience addresses questions on neural information processing by engineering reproducible biological neural networks of defined network topology in vitro. By using high-density CMOS MEAs, it enables high spatiotemporal resolution for sub-cellular readout and stimulation, overcoming the limitations of currently used glass MEAs.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Jose C. Mateus, Sean Weaver, Dirk van Swaay, Aline F. Renz, Julian Hengsteler, Paulo Aguiar, Janos Voeroes
Summary: This research utilizes electron beam lithography and photolithography to create microstructures that control neuronal connectivity, and demonstrates the formation of functional synapses in vitro.
Article
Biophysics
Stephan J. Ihle, Sophie Girardin, Thomas Felder, Tobias Ruff, Julian Hengsteler, Jens Duru, Sean Weaver, Csaba Forro, Janos Voros
Summary: We present a paradigm to examine the behavior of multiple neuronal networks with controlled topology in vitro. Our approach enables simultaneous electrical stimulation and recording of neuronal activity from 60 independent networks over multiple weeks. We found that the network response pattern to a stimulus remains stable for at least 12 hours and continuous stimulation does not significantly change the stimulation-induced mean spiking frequency of a circuit. We also investigated the effect of stimulation amplitude and timing on the network response and demonstrated the capability of our setup to apply complex stimulation protocols.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Jens Duru, Benedikt Maurer, Ciara Giles Doran, Robert Jelitto, Joel Kuchler, Stephan J. Ihle, Tobias Ruff, Robert John, Barbara Genocchi, Janos Voros
Summary: This article presents a bottom-up neuroscience approach using engineered biological neural networks to study neuronal activity. The researchers establish a platform with up to six independent networks formed by rat neurons on microelectrode arrays. They demonstrate that continuous application of a repetitive super-threshold stimulus generates a reproducible network response within a 15 ms post-stimulus window, which can be tracked with high spatiotemporal resolution across the entire network.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemical Research Methods
Sophie Girardin, Blandine Clement, Stephan J. Ihle, Sean Weaver, Jana B. Petr, Jose C. Mateus, Jens Duru, Csaba Forro, Tobias Ruff, Isabelle Fruh, Matthias Mueller, Janos Voeroes, Magdalena
Summary: Building small and controlled circuits of human induced pluripotent stem cell-derived neurons holds great promise in understanding the fundamental principles of information processing and storing in the brain.