Article
Virology
Kang Ning, Anuradha Roy, Fang Cheng, Peng Xu, Steve Kleiboeker, Carlos R. Escalante, Jingxin Wang, Jianming Qiu
Summary: This study developed a high-throughput screening assay for identifying antivirals against B19V replication and discovered a purine derivative that exhibited significant antiviral activity against B19V infection.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Wei Zou, Kang Ning, Peng Xu, Xuefeng Deng, Fang Cheng, Steve Kleiboeker, Jianming Qiu
Summary: The study revealed that the 5-68 amino acid sequence of VP1 can effectively inhibit B19V infection, independent of dimerization but likely dependent on three predicted alpha-helices. This specific sequence may function as a receptor-binding ligand during virus entry, making it a potential antiviral peptide candidate for combating B19V infection.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Kang Ning, Wei Zou, Peng Xu, Fang Cheng, Elizabeth Yan Zhang, Aaron Zhang-Chen, Steve Kleiboeker, Jianming Qiu
Summary: The study identified AXL as a protein receptor for B19V to infect human erythroid progenitor cells. Knocking down the AXL gene significantly reduced B19V internalization and replication. The interaction between B19V VP1u and the extracellular domain of AXL was confirmed, suggesting the importance of AXL as a co-receptor for B19V infection.
Article
Immunology
Yedong Yu, Chunchun Wei, Junhao Lyu, Xiaoliang Wu, Rending Wang, Hongfeng Huang, Jianyong Wu, Jianghua Chen, Wenhan Peng
Summary: This retrospective study evaluated donor-derived B19V infections in kidney transplantations from January 2016 to December 2020. Results showed 0.4% and 1.5% incidence rates in living and deceased donor kidney transplantations, respectively. While some recipients developed infections, they were effectively controlled with treatment, indicating that routine screening for B19V in donors may not be necessary and kidneys from donors with B19V infection can be accepted for transplantation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Jonathan L. Sanchez, Niloofar Ghadirian, Nancy C. Horton
Summary: This paper presents two new structures of the N-terminal domain of the main replication protein, NS1, from human parvovirus B19 (B19V). The NS1-nuc domain plays a crucial role in the rolling hairpin replication of the B19V DNA genome by recognizing origin of replication sequences in double-stranded DNA and cleaving single-stranded DNA at the terminal resolution site (trs). The high-resolution structures of NS1-nuc provide insights into DNA recognition and cleavage by B19V NS1-nuc and predict the important residues involved in DNA cleavage and sequence-specific recognition at the viral origin of replication.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Kang Ning, Zekun Wang, Fang Cheng, Ziying Yan, Jianming Qiu
Summary: This study identified the direct interactions between human bocavirus 1 (HBoV1) NP1 and Ku70/RPA70 and found that these interactions play a significant role in HBoV1 DNA replication.
Article
Biochemistry & Molecular Biology
Yixi Zhang, Zhiwei Shao, Yanqing Gao, Boming Fan, Jie Yang, Xi Chen, Xin Zhao, Qiyuan Shao, Weizhen Zhang, Chulei Cao, Hehua Liu, Jianhua Gan
Summary: Infection of human parvovirus B19 can lead to various diseases, and the NS1_Nuc protein plays a crucial role in viral DNA replication through its unique binding mode with single-stranded DNA.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Oncology
Li Gong, Jing Tian, Yan Zhang, Zheng Feng, Qiannan Wang, Yan Wang, Fuqin Zhang, Wei Zhang, Gaosheng Huang
Summary: The presence of germinal centers (GCs) in thymomas is closely associated with the pathogenesis of myasthenia gravis (MG), and infection by human parvovirus B19 (B19V) may contribute to the formation of ectopic GCs in thymoma. This study provides new insights into the etiopathogenic mechanism of MG and the role of B19V in thymoma-associated MG.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Immunology
Junshuang Guo, Yating Wang, Mian Zhang, Hongxiang Zheng, Qiuling Zang, Peipei Huang, Lijun Wen, Dandan Song, Fan Yang, Ruirui Dong, Wang Miao
Summary: A retrospective analysis of hospitalized patients suspected of microbial infection revealed a HPV B19 infection rate of 2.22%. Most infected patients had a history of decreased immunity and blood transfusion. NGS technology can detect HPV B19 in various bodily fluids and tissues. Severe infections may lead to nervous system damage. Early diagnosis and treatment can improve prognosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Domitille Callon, Fatma Berri, Anne-Laure Lebreil, Paul Fornes, Laurent Andreoletti
Summary: The co-infection of Parvovirus-B19 and Influenza A/H1N1 virus may lead to acute myocarditis, resulting in severe and fatal outcomes. The viruses exhibited different manifestations in the heart and lungs, but the specific mechanisms of viral interactions are not fully understood.
Article
Virology
Dayakar Seetha, Heera R. Pillai, Sai Ravi Chandra Nori, Sanu Ghosh Kalpathodi, Vineetha P. Thulasi, Radhakrishnan R. Nair
Summary: The study focused on the genetic and evolutionary characteristics of Human parvovirus B19V in South India by screening 456 suspected cases. Among them, 33 cases were found positive for B19V infection, with the South Indian strain showing three novel mutations in the VP1/2 gene. The research contributes to a better understanding of the virus in the region and suggests possible positive selection pressure on VP1/2 that could enhance virus survival and replication capabilities.
Article
Microbiology
Qian Du, Xuezhi Zhang, Ning Xu, Mengyu Ma, Bicheng Miao, Yong Huang, Dewen Tong
Summary: This study identified CCT5 as a host interacting protein of PPV NS1 and showed that CCT5 mediates the interaction of PPV NS1 and COPE, regulating viral replication.
VETERINARY MICROBIOLOGY
(2022)
Article
Immunology
Chunlin Xiang, Xiaoxiao Wu, Youkang Wei, Tianlong Li, Xuemei Tang, Yi Wang, Xiaoqin Zhang, Xiaobo Huang, Yiping Wang
Summary: This article reports a rare case of severe septic shock caused by human parvovirus B19 infection after cardiac surgery. The virus can lead to septicemia, rash, fever, and other symptoms, especially in immunocompromised patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Daniel Almeida do Valle, Mara Lucia S. Ferreira Santos, Monica J. Spinosa, Bruno A. Telles, Carolina Prando, Mara L. Cordeiro
Summary: This case study describes a 6-year-old boy hospitalized with super-refractory status epilepticus related to autoimmune encephalitis associated with B19 infection. Following immunosuppressive therapy, the patient achieved complete symptom remission, normal neurological examination, and appropriate neuropsychomotor development, highlighting the importance of early clinical suspicion and treatment.
Article
Virology
Heinz-Peter Schultheiss, Claus-Thomas Bock, Ganna Aleshcheva, Christian Baumeier, Wolfgang Poller, Felicitas Escher
Summary: This study aimed to evaluate the effects of interferon beta-1b (IFN-beta) treatment on the replicative status of human parvovirus B19 (B19V) and its beneficial effects. The results showed that IFN-beta treatment significantly reduced B19V viral RNA levels and improved cardiac function.
Editorial Material
Biotechnology & Applied Microbiology
Susan F. Cotmore, Mavis Agbandje-McKenna, Marta Canuti, John A. Chiorini, Anna-Maria Eis-Hubinger, Joseph Hughes, Mario Mietzsch, Sejal Modha, Mylene Ogliastro, Judit J. Penzes, David J. Pintel, Jianming Qiu, Maria Soderlund-Venermo, Peter Tattersall, Peter Tijssen, Elliot J. Lefkowitz, Andrew J. Davison, Stuart G. Siddell, Peter Simmonds, Sead Sabanadzovic, Donald B. Smith, Richard J. Orton, Balazs Harrach
JOURNAL OF GENERAL VIROLOGY
(2019)
Article
Virology
Xiaomei Wang, Peng Xu, Fang Cheng, Yi Li, Zekun Wang, Siyuan Hao, Jianke Wang, Kang Ning, Safder S. Ganaie, John F. Engelhardt, Ziying Yan, Jianming Qiu
JOURNAL OF VIROLOGY
(2020)
Letter
Medicine, General & Internal
Yuntao Wu, Wenzhe Ho, Yaowei Huang, Dong-Yan Jin, Shiyue Li, Shan-Lu Liu, Xuefeng Liu, Jianming Qiu, Yongming Sang, Qiuhong Wang, Kwok-Yung Yuen, Zhi-Ming Zheng
Article
Genetics & Heredity
Xuefeng Deng, Wei Zou, Ziying Yan, Jianming Qiu
Article
Virology
Junxing Zhao, Jianming Qiu, Sadikshya Aryal, Jennifer L. Hackett, Jingxin Wang
Article
Multidisciplinary Sciences
Mijia Lu, Piyush Dravid, Yuexiu Zhang, Sheetal Trivedi, Anzhong Li, Olivia Harder, K. C. Mahesh, Supranee Chaiwatpongsakorn, Ashley Zani, Adam Kenney, Cong Zeng, Chuanxi Cai, Chengjin Ye, Xueya Liang, Masako Shimamura, Shan-Lu Liu, Asuncion Mejias, Octavio Ramilo, Prosper N. Boyaka, Jianming Qiu, Luis Martinez-Sobrido, Jacob S. Yount, Mark E. Peeples, Amit Kapoor, Stefan Niewiesk, Jianrong Li
Summary: Using a measles virus vaccine strain as the backbone, researchers developed a series of recombinant attenuated vaccine candidates expressing various forms of the SARS-CoV-2 spike protein. Among these candidates, rMeV expressing stabilized prefusion S protein showed the highest potency in inducing neutralizing antibodies and provided complete protection against SARS-CoV-2 in animal models. This supports further development of rMeV-preS as a highly effective vaccine candidate against SARS-CoV-2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Mijia Lu, Yuexiu Zhang, Piyush Dravid, Anzhong Li, Cong Zeng, K. C. Mahesh, Sheetal Trivedi, Himanshu Sharma, Supranee Chaiwatpongsakorn, Ashley Zani, Adam Kenney, Chuanxi Cai, Chengjin Ye, Xueya Liang, Jianming Qiu, Luis Martinez-Sobrido, Jacob S. Yount, Prosper N. Boyaka, Shan-Lu Liu, Mark E. Peeples, Amit Kapoor, Jianrong Li
Summary: The study developed a recombinant vesicular stomatitis virus (mtdVSV)-based SARS-CoV-2 vaccine candidate, which was proven to be safe and highly efficacious in mouse and hamster experiments. It induced high levels of neutralizing antibodies and showed promise in providing protection against SARS-CoV-2 infection.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Yisong Qian, Tianhua Lei, Parth S. Patel, Chi H. Lee, Paula Monaghan-Nichols, Hong-Bo Xin, Jianming Qiu, Mingui Fu
Summary: Evidence suggests that SARS-CoV-2's NP significantly activates human endothelial cells through TLR2/NF-κB and MAPK signaling pathways, with simvastatin identified as a potential inhibitor. This study provides insights on COVID-19-induced vasculopathy and coagulopathy, emphasizing the importance of understanding the mechanistic role of vasculature in these patients.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Zekun Wang, Songyang Ren, Qiming Li, Austin D. Royster, Lei Lin, Sichen Liu, Safder S. Ganaie, Jianming Qiu, Sheema Mir, Mohammad A. Mir
Summary: The Hantavirus nucleocapsid protein (NP) inhibits protein kinase R (PKR) by using an endogenous PKR inhibitor P58(IPK) to compete with its negative regulator Hsp40, releasing P58(IPK) and selectively engaging it on the 40S ribosomal subunit.
Article
Virology
Renuk Lakshmanan, Mario Mietzsch, Alberto Jimenez Ybargollin, Paul Chipman, Xiaofeng Fu, Jianming Qiu, Maria Soderlund-Venermo, Robert McKenna
Summary: This study determined the capsid structures of Aleutian mink disease virus (AMDV) and human parvovirus 4 (PARV4) using cryo-electron microscopy. Despite low amino acid sequence similarities, both viruses have icosahedral capsid structures and display major structural variabilities in surface loops.
Article
Virology
Siyuan Hao, Kang Ning, Cagla A. Kuz, Min Xiong, Wei Zou, Soo Y. Park, Shane McFarlin, Ziying Yan, Jianming Qiu
Summary: This study reveals that SARS-CoV-2 infection triggers necroptosis in human airway epithelia in vitro, disrupting the barrier function. Pharmacological inhibitors and gene silencing can effectively reduce cell death and epithelial barrier damage caused by the infection.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Siyuan Hao, Xiujuan Zhang, Kang Ning, Zehua Feng, Soo Yeun Park, Cagla Aksu Kuz, Shane McFarlin, Donovan Richart, Fang Cheng, Elizabeth Yan Zhang, Aaron Zhang-Chen, Ziying Yan, Jianming Qiu
Summary: rAAV2.5T, selected through directed evolution, is capable of efficiently transducing human airway epithelium. Our study revealed that KIAA0319L is not essential for vector internalization but plays a critical role in efficient vector transduction to human airway epithelium. We also identified a novel gene, WDR63, which is important for vector transduction of human airway epithelium but not for vector internalization and nuclear entry. Additionally, our study discovered the significant transduction potential of rAAV2.5T in the basal stem cells of human airway epithelium, highlighting its utility in gene editing for pulmonary genetic diseases.
JOURNAL OF VIROLOGY
(2023)
Article
Medicine, Research & Experimental
Kang Ning, Xiujuan Zhang, Zehua Feng, Siyuan Hao, Cagla Aksu Kuz, Fang Cheng, Soo Yuen Park, Shane Mcfarlin, John F. Engelhardt, Ziying Yan, Jianming Qiu
Summary: This study found that DNA damage response is associated with rAAV transduction in human airway epithelia, and DNA-PKcs inhibition has the potential to increase rAAV transduction.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Virology
Liting Shao, Kang Ning, Jianke Wang, Fang Cheng, Shengqi Wang, Jianming Qiu
Summary: In this study, we discovered a direct interaction between the large nonstructural protein NS1 of parvovirus and Ku70, which is critical for HBoV1 replication.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Siyuan Hao, Kang Ning, Cagla Aksu Kuz, Kai Vorhies, Ziying Yan, Jianming Qiu