Article
Microbiology
Ning Shi, Shu Zhang, Yidi Guo, Xiuhua Yu, Weizheng Zhao, Maolin Zhang, Zhenhong Guan, Ming Duan
Summary: Circular RNAs (circRNAs) are a type of endogenous regulatory RNAs that play important roles in cellular responses to influenza A virus (IAV) infection, promoting viral replication. Specifically, circ_0050463 acts as a microRNA-33b-5p sponge to inhibit miR-33b-5p activity, leading to increased EEF1A1 expression and facilitating IAV replication.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Lulu Deng, Lingcai Zhao, Jiayu Jin, Bingchen Qiao, Xiaoting Zhang, Lifeng Chang, Lucheng Zheng, Samar Dankar, Jihui Ping
Summary: The overexpression of TACC3 reduces the viral titers of multiple influenza A viruses, while the downregulation of TACC3 increases viral propagation. TACC3's inhibitory effect on viral replication occurs in the early stages, as its overexpression leads to decreased viral nucleoprotein accumulation and slower endosomal trafficking. Overall, TACC3 acts as an important inhibitory factor for influenza A virus replication.
VETERINARY MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Liuke Sun, Huihui Kong, Mengmeng Yu, Zhenyu Zhang, Haili Zhang, Lei Na, Yuxing Qu, Yuan Zhang, Hualan Chen, Xiaojun Wang
Summary: Species differences in ANP32A/B restrict avian influenza virus in mammalian cells. Adaptive mutations, such as PB2-E627K, are required for efficient replication of avian influenza viruses in mammals. We found that NS2 protein promotes avian vRNP assembly and enhances interactions with mammalian ANP32A/B, overcoming the restriction. Disrupting the conserved SUMO-interacting motif (SIM) in NS2 impairs virus replication and pathogenicity in mammals, indicating NS2 as a cofactor in the adaptation process of avian influenza virus to mammals.
Article
Fisheries
Xiaodong Zhang, Kai Hao, Shuxin Li, Lihui Meng, Hongxun Chen, Fucheng Wei, Fei Yu, Jing Xu, Zhe Zhao
Summary: This study investigated the molecular mechanisms of CCV infection and genome replication. The results showed that ORF25 and ORF63 are essential for regulating CCV genome replication and infection.
JOURNAL OF FISH DISEASES
(2022)
Article
Microbiology
Jan Bieri, Remo Leisi, Cornelia Bircher, Carlos Ros
Summary: The study found that glycosphingolipid globoside (Gb4) does not participate in the binding or internalization of parvovirus B19 (B19V) into permissive erythroid cells, but is essential for a post-internalization step before the delivery of viral DNA into the nucleus.
Article
Microbiology
Yuting Shi, Jingwan Han, Bo Zhu, Zhi Liu, Qingmiao Liang, Chunlin Lan, Zhengyang Li, Hanping Li, Yongjian Liu, Lei Jia, Tianyi Li, Xiaolin Wang, Jingyun Li, Bohan Zhang, Junjun Jiang, Lin Li
Summary: This study identifies the subtype-specific loci of HIV-1 subtype B RRE circulating in China and examines their impact on viral replication and Rev-RRE activity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Weigang Ren, Shuli Pei, Wenming Jiang, Meixia Zhao, Le Jiang, Honggang Liu, Yongxiang Yi, Mizhou Hui, Junwei Li
Summary: A replication-deficient recombinant influenza virus was developed in this study, which stimulated robust immune response and conferred complete protection against H5N1 or H9N2 influenza virus infections in mice.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sergi Chumillas, Saurabh Loharch, Manuela Beltran, Mateusz P. Szewczyk, Silvia Bernal, Maria C. Puertas, Javier Martinez-Picado, Jose Alcami, Luis M. Bedoya, Vicente Marchan, Jose Gallego
Summary: This study explored the optimization of the antiretroviral properties of benfluron and found that both the benzo[c] and cyclopentanone components are essential for its activity. The results provide insights for the design of new anti-HIV agents targeting the inhibition of Rev function.
Article
Biochemistry & Molecular Biology
Young Hyun Park, Seung Je Woo, Kelly Chungu, Su Bin Lee, Ji Hyeon Shim, Hong Jo Lee, Iktae Kim, Deivendran Rengaraj, Chang-Seon Song, Jeong-Yong Suh, Jeong Mook Lim, Jae Yong Han
Summary: The study found that the 27 residues of human ANP32A play a crucial role in AIV vPol activity, while ANP32C cannot support this activity. Asp149 and Asp152 are involved in supporting vPol activity, and mutations in these residues decreases the interaction between ANP32A and vPol.
Article
Virology
Lei Zhang, Yuekun Shao, Yingying Wang, Qiuxian Yang, Jiamei Guo, George F. Gao, Tao Deng
Summary: The last amino acid I121 of the viral NS2 protein plays a critical role in promoting viral genome replication. The hydrophobicity of residue 121 is essential for virus survival. Adaptive mutations PA-K19E and PB1-S713N compensate for the replication-promoting defect caused by NS2-I121 mutation.
JOURNAL OF VIROLOGY
(2023)
Article
Chemistry, Medicinal
Lin Tang, Haiyan Yan, Weibin Wu, Dawei Chen, Zhenxiong Gao, Jinqiang Hou, Cunlong Zhang, Yuyang Jiang
Summary: A series of novel substituted polycyclic pyridone derivatives were synthesized and found to exhibit potent anti-influenza activity, including inhibiting the replication of influenza A virus at picomolar concentrations. These compounds showed low cytotoxicity and promising potential as anti-influenza drug candidates.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Yunyun Cheng, Chaofu Yang, Zhan Li, Xiheng Li, Xiaocui Zou, Lei Li, Menghan Cui, Airong Tian, Xinyu Li, Wei He, Zhongpeng Zhao, Yongsheng Ding
Summary: Eight SR9009 analogues were synthesized and evaluated for their antiviral activities against multiple influenza virus strains. Compound 5a showed the most potent inhibitory activity and upregulated key antiviral interferon-stimulated genes.
ANTIVIRAL RESEARCH
(2022)
Article
Virology
Shufang Fan, Chunyang Gu, Huihui Kong, Lizheng Guan, Gabriele Neumann, Yoshihiro Kawaoka
Summary: Small animal models are crucial for studying the properties of seasonal and pandemic influenza viruses, but they do not always replicate human influenza viruses efficiently. Researchers have developed a high-yield vaccine virus backbone that can increase the replication of seasonal influenza viruses in Syrian hamsters, providing a useful tool for evaluating the biological properties of influenza viruses.
Article
Chemistry, Multidisciplinary
Mathilde Briday, Francois Halle, Lauriane Lecoq, Sylvie Radix, Juliette Martin, Roland Montserret, Marie Dujardin, Marie-Laure Fogeron, Michael Nassal, Beat H. Meier, Thierry Lomberget, Anja Bockmann
Summary: Hepatitis B virus (HBV) is a significant human pathogen and its capsid-forming core protein (Cp) plays a key role in envelopment. Recent studies have found that Triton X-100, a detergent used in Cp purification, strongly binds to the hydrophobic pocket of Cp. The aromatic moiety is crucial for binding, while the aliphatic chain affects binding affinity. Moreover, the hydrophilic tail shows high tolerance to changes in length and type.
Article
Biochemistry & Molecular Biology
Dongyu Zhao, Min Zhang, Shaodong Huang, Qi Liu, Sen Zhu, Yanqiang Li, Weihua Jiang, Daniel L. Kiss, Qi Cao, Lili Zhang, Kaifu Chen
Summary: In this study, elevated expression of CHD6 in prostate cancer is found to be associated with poor prognosis. CHD6 regulates the oncogenicity of prostate cancer cells by evicting nucleosomes and activating prostate cancer pathways.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Jason E. Shoemaker, Satoshi Fukuyama, Amie J. Eisfeld, Dongming Zhao, Eiryo Kawakami, Saori Sakabe, Tadashi Maemura, Takeo Gorai, Hiroaki Katsura, Yukiko Muramoto, Shinji Watanabe, Tokiko Watanabe, Ken Fuji, Yukiko Matsuoka, Hiroaki Kitano, Yoshihiro Kawaoka
Article
Biochemical Research Methods
Zhen Niu, Deborah Chasman, Amie J. Eisfeld, Yoshihiro Kawaoka, Sushmita Roy
Article
Mathematical & Computational Biology
Jason E. McDermott, Hugh D. Mitchell, Lisa E. Gralinski, Amie J. Eisfeld, Laurence Josset, Armand Bankhead, Gabriele Neumann, Susan C. Tilton, Alexandra Schafer, Chengjun Li, Shufang Fan, Shannon McWeeney, Ralph S. Baric, Michael G. Katze, Katrina M. Waters
BMC SYSTEMS BIOLOGY
(2016)
Article
Microbiology
Jennifer Tisoncik-Go, David J. Gasper, Jennifer E. Kyle, Amie J. Eisfeld, Christian Selinger, Masato Hatta, Juliet Morrison, Marcus J. Korth, Erika M. Zink, Young-Mo Kim, Athena A. Schepmoes, Carrie D. Nicora, Samuel O. Purvine, Karl K. Weitz, Xinxia Peng, Richard R. Green, Susan C. Tilton, Bobbie-Jo Webb-Robertson, Katrina M. Waters, Thomas O. Metz, Richard D. Smith, Yoshihiro Kawaoka, M. Suresh, Laurence Josset, Michael G. Katze
CELL HOST & MICROBE
(2016)
Article
Immunology
Danielle Westhoff Smith, Lindsay Hill-Batorski, Alhaji N'jai, Amie J. Eisfeld, Gabriele Neumann, Peter Halfmann, Yoshihiro Kawaoka
JOURNAL OF INFECTIOUS DISEASES
(2016)
Article
Biochemical Research Methods
Deborah Chasman, Kevin B. Walters, Tiago J. S. Lopes, Amie J. Eisfeld, Yoshihiro Kawaoka, Sushmita Roy
PLOS COMPUTATIONAL BIOLOGY
(2016)
Editorial Material
Biochemistry & Molecular Biology
Amie J. Eisfeld, Yoshihiro Kawaoka
Article
Multidisciplinary Sciences
Vineet D. Menachery, Alexandra Schafer, Kristin E. Burnum-Johnson, Hugh D. Mitchell, Amie J. Eisfeld, Kevin B. Walters, Carrie D. Nicora, Samuel O. Purvine, Cameron P. Casey, Matthew E. Monroe, Karl K. Weitz, Kelly G. Stratton, Bobbie-Jo M. Webb-Robertson, Lisa E. Gralinski, Thomas O. Metz, Richard D. Smith, Katrina M. Waters, Amy C. Sims, Yoshihiro Kawaoka, Ralph S. Baric
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Microbiology
Amie J. Eisfeld, David J. Gasper, M. Suresh, Yoshihiro Kawaoka
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
J. E. Kyle, K. E. Burnum-Johnson, J. P. Wendler, A. J. Eisfeld, Peter J. Halfmann, Tokiko Watanabe, Foday Sahr, R. D. Smith, Y. Kawaoka, K. M. Waters, T. O. Metz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Infectious Diseases
Peter J. Halfmann, Amie J. Eisfeld, Tokiko Watanabe, Tadashi Maemura, Makoto Yamashita, Satoshi Fukuyama, Tammy Armbrust, Isaiah Rozich, Alhaji N'jai, Gabriele Neumann, Yoshihiro Kawaoka, Foday Sahr
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Cell Biology
Hugh D. Mitchell, Amie J. Eisfeld, Kelly G. Stratton, Natalie C. Heller, Lisa M. Bremer, Ji Wen, Jason E. McDermott, Lisa E. Gralinski, Amy C. Sims, Mai Q. Le, Ralph S. Baric, Yoshihiro Kawaoka, Katrina M. Waters
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Lisa M. Bramer, Robert D. Hontz, Amie J. Eisfeld, Amy C. Sims, Young-Mo Kim, Kelly G. Stratton, Carrie D. Nicora, Marina A. Gritsenko, Athena A. Schepmoes, Osamu Akasaka, Michiko Koga, Takeya Tsutsumi, Morio Nakamura, Ichiro Nakachi, Rie Baba, Hiroki Tateno, Shoji Suzuki, Hideaki Nakajima, Hideaki Kato, Kazunari Ishida, Makoto Ishii, Yoshifumi Uwamino, Keiko Mitamura, Vanessa L. Paurus, Ernesto S. Nakayasu, Isaac K. Attah, Andrew G. Letizia, Katrina M. Waters, Thomas O. Metz, Karen Corson, Yoshihiro Kawaoka, Vincent R. Gerbasi, Hiroshi Yotsuyanagi, Kiyoko Iwatsuki-Horimoto
Summary: To better understand the pathology of COVID-19, a multi-omics and correla-tive analysis was conducted on a clinical cohort. Severe patients exhibited elevated levels of pulmonary surfactant, while mild patients showed an increase in the carnosine dipeptidase modifying enzyme (CNDP1). Both mild and severe patients had elevated L-cystine and enzymes related to glutathione metabolism. Neutrophil extracellular traps (NETs) were observed in both patient groups, with higher formation in severe cases. CNDP1 may play a protective role in suppressing PSPB release, while NET formation is correlated with increased PSPB levels and disease severity, potentially leading to pulmonary occlusions.
Article
Microbiology
Amie J. Eisfeld, Peter J. Halfmann, Jason P. Wendler, Jennifer E. Kyle, Kristin E. Burnum-Johnson, Zuleyma Peralta, Tadashi Maemura, Kevin B. Walters, Tokiko Watanabe, Satoshi Fukuyama, Makoto Yamashita, Jon M. Jacobs, Young-Mo Kim, Cameron P. Casey, Kelly G. Stratton, Bobbie-Jo M. Webb-Robertson, Marina A. Gritsenko, Matthew E. Monroe, Karl K. Weitz, Anil K. Shukla, Mingyuan Tian, Gabriele Neumann, Jennifer L. Reed, Harm van Bakel, Thomas O. Metz, Richard D. Smith, Katrina M. Waters, Alhaji N'jai, Foday Sahr, Yoshihiro Kawaoka
CELL HOST & MICROBE
(2017)
Article
Chemistry, Analytical
Kristin E. Burnum-Johnson, Jennifer E. Kyle, Amie J. Eisfeld, Cameron P. Casey, Kelly G. Stratton, Juan F. Gonzalez, Fabien Habyarimana, Nicholas M. Negretti, Amy C. Sims, Sadhana Chauhan, Larissa B. Thackray, Peter J. Halfmann, Kevin B. Walters, Young-Mo Kim, Erika M. Zink, Carrie D. Nicora, Karl K. Weitz, Bobbie-Jo M. Webb-Robertson, Ernesto S. Nakayasu, Brian Ahmer, Michael E. Konkel, Vladimir Motin, Ralph S. Baric, Michael S. Diamond, Yoshihiro Kawaoka, Katrina M. Waters, Richard D. Smith, Thomas O. Metz