Article
Multidisciplinary Sciences
Daniel J. Emerson, Peiyao A. Zhao, Ashley L. Cook, R. Jordan Barnett, Kyle N. Klein, Dalila Saulebekova, Chunmin Ge, Linda Zhou, Zoltan Simandi, Miriam K. Minsk, Katelyn R. Titus, Weitao Wang, Wanfeng Gong, Di Zhang, Liyan Yang, Sergey V. Venev, Johan H. Gibcus, Hongbo Yang, Takayo Sasaki, Masato T. Kanemaki, Feng Yue, Job Dekker, Chun-Long Chen, David M. Gilbert, Jennifer E. Phillips-Cremins
Summary: This article reveals the determinants of replication origin positioning in the human genome. The study found that topologically associating domains and loops play crucial roles in the localization of replication initiation zones. By disrupting cohesin-mediated loop extrusion, the positioning of replication origins can be altered. These findings contribute to a better understanding of the mechanism of DNA replication.
Article
Biochemistry & Molecular Biology
Yunying Zhou, Jason J. Chen
Summary: STAT3 plays an important role in DNA replication, mediated by its newly identified target gene WDHD1. These studies have significant implications.
CELL AND BIOSCIENCE
(2021)
Article
Microbiology
Simran Khurana, Tovah E. Markowitz, Juraj Kabat, Alison A. McBride
Summary: The life cycle of human papillomavirus (HPV) relies on keratinocyte differentiation to replicate and assemble its genome in cellular pathways, with cellular histones and viral proteins playing crucial roles in this process.
Article
Multidisciplinary Sciences
Hannah J. Richter, Amy K. Hauck, Kirill Batmanov, Shin-Ichi Inoue, Bethany N. So, Mindy Kim, Matthew J. Emmett, Ronald N. Cohen, Mitchell A. Lazar
Summary: Brown adipose tissue (BAT) relies on the interaction between nuclear receptor corepressors (NCoRs) and histone deacetylase 3 (HDAC3) for thermogenesis and lipid metabolism. Loss of NCoRs leads to inflammation in BAT, while loss of HDAC3 causes cold sensitivity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Alison A. McBride, Alix Warburton, Simran Khurana
Summary: HPV reproduces in stratified epithelia by establishing a reservoir of low-level infection in dividing basal cells and restricting viral particle production to terminally differentiated cells. The Brd4 protein plays a key role in multiple stages of the HPV infectious cycle.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Commodore St Germain, Hongchang Zhao, Jacqueline H. Barlow
Summary: Transcription-replication interactions are essential processes in life, but they also pose potential threats to genome integrity due to obstacles such as R-loops, DNA supercoiling, DNA secondary structure, and chromatin-binding proteins. It is critical to maintain high fidelity and processivity of transcription and replication in a complex chromatin environment to avoid these threats. Various safeguards have evolved to navigate transcription-replication interactions and maintain genome integrity in mammalian cells.
Article
Oncology
Jun Wang, Aftab Amin, Man Hei Cheung, Linjing Shi, Chun Liang
Summary: MicroRNAs are noncoding RNAs that can suppress gene expression by degrading target mRNA or impairing translation. MCM proteins, essential for DNA replication, are overexpressed in cancer cells but strictly regulated in normal cells. miRNA-214-3p targets MCM5 and MCM7, inhibiting DNA replication and cell proliferation.
Article
Oncology
Jessica Marinello, Andrea Arleo, Marco Russo, Maria Delcuratolo, Francesca Ciccarelli, Yves Pommier, Giovanni Capranico
Summary: This study used molecular, genetic, and bioinformatic approaches to investigate the mechanism of micronuclei formation induced by TOP1 poisons in different cancer cells. The results revealed an immune signaling mechanism activated by TOP1 poisons, which is often impaired in human small-cell lung cancer tumors.
BRITISH JOURNAL OF CANCER
(2022)
Article
Cell Biology
Perumal Arumugam Desingu, Sneha Mishra, Lavanya Dindi, Shalini Srinivasan, Raju S. Rajmani, Venkatraman Ravi, Ankit Kumar Tamta, Sukanya Raghu, Krishnega Murugasamy, Anwit Shriniwas Pandit, Nagalingam R. Sundaresan
Summary: This study demonstrates that inhibition of PARP1 can protect against Japanese encephalitis virus (JEV) infection by inhibiting autophagy.
Article
Multidisciplinary Sciences
Xing Liu, Dhiraj Acharya, Eric Krawczyk, Chase Kangas, Michaela U. Gack, Bin He
Summary: This study found that viral gamma(1)34.5 protein interacts with ICP0 to prevent its degradation, while host E3 ligase TRIM23 triggers proteasomal degradation of ICP0. The gamma(1)34.5 protein disables TRIM23, which down-regulates ICP0 and ultimately determines the outcome of HSV-1 infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Physics, Fluids & Plasmas
Dean Huang, Teresa Lo, Houra Merrikh, Paul A. Wiggins
Summary: This paper introduces two commonly used experimental approaches for studying the cell cycle and cell biology. The authors generalize a deterministic model of cell-cycle dynamics to a stochastic model and explore the relationship between the two models. They find that even if the timing of cell states is stochastic, the demographics of an exponentially growing population can be well fit by a deterministic model.
Article
Microbiology
Deani L. Cooper, Taku Harada, Samia Tamazi, Alexander E. Ferrazzoli, Susan T. Lovett, James Keck
Summary: In Escherichia coli, DNA replication is catalyzed by the DNA polymerase III holoenzyme, including the non-essential accessory protein HolC, which when mutated can lead to growth impairment, genetic instability, and sensitivity to DNA damaging agents. The study identified suppressor alleles of RNA polymerase and sspA that partially suppress the loss of HolC, while mutations in transcription factors DksA and NusA exacerbate the inviability of HolC mutants. The findings suggest that transcription complexes may block replication in HolC mutants, and Rho-dependent transcriptional termination and DksA function are crucial for sustaining viability and chromosome integrity in these mutants.
Article
Biochemistry & Molecular Biology
Chih-Hao Huang, Yu-Chang Liu, Jia-Yi Shen, Fu- Lu, Shyh-Yu Shaw, Hao-Jen Huang, Ching-Chun Chang
Summary: The study developed a cpTALEN technology for chloroplast DNA editing and successfully achieved site-specific editing of the rpoB gene in tobacco leaves. Transgenic plants exhibited varying degrees of chlorotic phenotype, and repairing damaged plastid DNA led to point mutations, large deletions, and small inversions surrounding the rpoB gene through homologous recombination and/or microhomology-mediated recombination.
Article
Chemistry, Multidisciplinary
Yudai Tabuchi, Jay Yang, Masumi Taki
Summary: This study created a DNA-aptamer-type covalent drug which can be deactivated and reactivated through a specific structure, providing a new approach to alleviate irreversible adverse drug effects.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Liming Yan, Yucen Huang, Ji Ge, Zhenyu Liu, Pengchi Lu, Bo Huang, Shan Gao, Junbo Wang, Liping Tan, Sihan Ye, Fengxi Yu, Weiqi Lan, Shiya Xu, Feng Zhou, Lei Shi, Luke W. Guddat, Yan Gao, Zihe Rao, Zhiyong Lou
Summary: This study reveals the mechanism of SARS-CoV-2 RNA capping and provides atomic resolution structural details. It shows that the NiRAN domain plays a crucial role in RNAylation and the formation of GpppA. Moreover, triphosphorylated nucleotide analog inhibitors can inhibit RNAylation and GpppA formation by binding to nsp9. The conformational shift of the S-loop in NiRAN observed in the presence of sofosbuvir monophosphate suggests a possible mechanism for designing inhibitors.
Article
Neurosciences
Ebru Ercan, Sameh Eid, Christian Weber, Alexandra Kowalski, Maria Bichmann, Annika Behrendt, Frank Matthes, Sybille Krauss, Peter Reinhardt, Simone Fulle, Dagmar E. Ehrnhoefer
MOLECULAR NEURODEGENERATION
(2017)
Article
Biochemistry & Molecular Biology
Frank Matthes, Serena Massari, Anna Bochicchio, Kenji Schorp, Judith Schilling, Stephanie Weber, Nina Offermann, Jemy Desantis, Erich Wanker, Paolo Carloni, Kamyar Hadian, Oriana Tabarrini, Giulia Rossetti, Sybille Krauss
ACS CHEMICAL NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Kristoffer Unterbruner, Frank Matthes, Judith Schilling, Rohit Nalavade, Stephanie Weber, Jennifer Winter, Sybille Krauss
Article
Multidisciplinary Sciences
Bernd O. Evert, Rohit Nalavade, Johannes Jungverdorben, Frank Matthes, Stephanie Weber, Ashish Rajput, Stefan Bonn, Oliver Bruestle, Michael Peitz, Sybille Krauss
Article
Multidisciplinary Sciences
Susann Schweiger, Frank Matthes, Karen Posey, Eva Kickstein, Stephanie Weber, Moritz M. Hettich, Sandra Pfurtscheller, Dan Ehninger, Rainer Schneider, Sybille Krauss
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Debora Kaminski, Claudia Yaghootfam, Frank Matthes, Annika Ressing, Volkmar Gieselmann, Ulrich Matzner
HUMAN MOLECULAR GENETICS
(2020)
Article
Peripheral Vascular Disease
Amra Jujic, Frank Matthes, Lotte Vanherle, Henning Petzka, Marju Orho-Melander, Peter M. Nilsson, Martin Magnusson, Anja Meissner
Summary: The study found an association between plasma S1P and elevated systolic blood pressure, validated in both a mouse model and in humans. Further proteomic analysis revealed multiple S1P associations, some of which were sex-specific. In vitro and in vivo validation showed increased expression of vascular dysfunction and inflammation markers in response to S1P.
Article
Biochemistry & Molecular Biology
Franziska E. Uhl, Lotte Vanherle, Frank Matthes, Anja Meissner
Summary: Changes in S1P signaling and CFTR expression are associated with heart failure and target organ damage. CFTR alterations during HF affect systemic and tissue-specific S1P concentrations. The CFTR-S1P axis plays an important role in HF-mediated systemic and pulmonary inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Isabel Goncalves, Loureen Oduor, Frank Matthes, Narjess Rakem, Jakob Meryn, Nikolaos-Taxiarchis Skenteris, Anders Aspberg, Marju Orho-Melander, Jan Nilsson, Ljubica Matic, Andreas Edsfeldt, Jiangming Sun, Eva Bengtsson
Summary: This study found that the expression of osteomodulin in human atherosclerotic plaques is associated with plaque calcification and plaque stability.
Article
Cardiac & Cardiovascular Systems
Dania Al-Sharify, Signe Holm Nielsen, Frank Matthes, Christoffer Tengryd, Jiangming Sun, Federica Genovese, Morten A. Karsdal, Jan Nilsson, Isabel Goncalves, Andreas Edsfeldt
Summary: This study investigated the association of cleaved osteoglycin with plaque phenotype and found that higher levels of cleaved osteoglycin were associated with a stable plaque phenotype and lower risk for future cardiovascular events. This association may be due to reduced cell apoptosis and the ability to retain LDL. Targeting the cleavage of osteoglycin may be a potential therapeutic strategy to stabilize plaques.
Article
Medicine, Research & Experimental
Lotte Vanherle, Frank Matthes, Franziska E. Uhl, Anja Meissner
Summary: CFTR dysfunction is associated with various conditions, including myocardial infarction. Therapeutic increase of CFTR expression can attenuate the associated effects. The effects of potentiating CFTR function post-MI are still unknown.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Cecilia Skoug, Hueseyin Erdogan, Lotte Vanherle, Joao P. P. Vieira, Frank Matthes, Lena Eliasson, Anja Meissner, Joao M. N. Duarte
Summary: S1P signaling is altered in synapses of insulin resistance and diet-induced obesity models, suggesting a role in T2D-associated synaptic dysfunction.
NEUROCHEMICAL RESEARCH
(2023)
Article
Geriatrics & Gerontology
Nicholas Don-Doncow, Lotte Vanherle, Frank Matthes, Sine Kragh Petersen, Hana Matuskova, Sara Rattik, Anetta Hartlova, Anja Meissner
Summary: Chronic hypercholesterolemia is associated with immune system activation and pro-inflammatory cell infiltration in the brain, leading to memory dysfunction in elderly mice. Therapeutic cholesterol-lowering with simvastatin can reduce inflammation and memory deficits in these mice, while blood pressure-lowering therapy alone may not be as effective in mitigating memory decline.
NPJ AGING AND MECHANISMS OF DISEASE
(2021)
Article
Cardiac & Cardiovascular Systems
Darcy Lidington, Jessica C. Fares, Franziska E. Uhl, Danny D. Dinh, Jeffrey T. Kroetsch, Meghan Sauv, Firhan A. Malik, Frank Matthes, Lotte Vanherle, Arman Adel, Abdul Momen, Hangjun Zhang, Roozbeh Aschar-Sobbi, Warren D. Foltz, Hoyee Wan, Manabu Sumiyoshi, R. Loch Macdonald, Mansoor Husain, Peter H. Backx, Scott P. Heximer, Anja Meissner, Steffen-Sebastian Bolz
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2019)
Article
Cell Biology
Frank Matthes, Moritz M. Hettich, Judith Schilling, Diana Flores-Dominguez, Nelli Blank, Thomas Wiglenda, Alexander Buntru, Hanna Wolf, Stephanie Weber, Ina Vorberg, Alina Dagane, Gunnar Dittmar, Erich Wanker, Dan Ehninger, Sybille Krauss
CELL DEATH DISCOVERY
(2018)