Article
Biochemistry & Molecular Biology
Agnieszka Szopa, Michal Dziurka, Pawel Kubica, Karolina Jafernik, Oliwia Siomak, Halina Ekiert
Summary: This study investigated the effect of different elicitors on lignan accumulation in microshoot cultures of female and male Schisandra rubriflora. The results showed that certain elicitors significantly increased lignan content.
Article
Agricultural Engineering
Rajendran K. Selvakesavan, Gregory Franklin
Summary: This article presents simple, robust, and species-independent methods for establishing in vitro cell suspension and shoot cultures of Hypericum plants, which are commonly used for treating depression. Stable cell suspension and shoot cultures were successfully established for all species tested, regardless of their habits and habitats, providing valuable insights for sustainable bioprospecting.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Dijana Krstic-Milosevic, Nevena Banjac, Teodora Jankovic, Dragan Vinterhalter, Branka Vinterhalter
Summary: In vitro shoot culture of the endangered medicinal plant Gentianella lutescens was established and different treatments were found to have an impact on shoot growth and xanthone production. Controlling the concentration of sucrose, sorbitol, and elicitors in the culture medium can significantly affect the accumulation of xanthones in shoot cultures.
Article
Plant Sciences
Zhuoya Liu, Wen-Lu Bi, Mukund R. Shukla, Praveen K. Saxena
Summary: This study developed a micropropagation and cryopreservation program using a liquid-based temporary immersion system for American chestnut trees, providing efficient strategies for species recovery and conservation.
Article
Plant Sciences
Lilyana Nacheva, Nataliya Dimitrova, Lyubka Koleva-Valkova, Miroslava Stefanova, Tsveta Ganeva, Marieta Nesheva, Ivan Tarakanov, Andon Vassilev
Summary: This study investigated the effects of LED light sources on the growth and rooting of plum rootstock Saint Julien. The results showed that mixed light (WBR) was the best choice during the multiplication phase, while red LED light was more suitable during the rooting stage.
Article
Biology
Merve Sifa Hane Kose, Muhammet Dogan, Gokhan Sadi
Summary: This study aimed to achieve multiple and rapid propagations of Staurogyne repens using tissue culture techniques. The shoot regeneration capabilities of the nodal explants were high due to the growth regulators used, with shoot regeneration frequencies ranging between 77.77% and 100.00% in different applications of BAP-IAA and KIN-IAA.
Article
Plant Sciences
Guojun Hu, Yafeng Dong, Zunping Zhang, Xudong Fan, Fang Ren
Summary: Shoot tip culture is an effective approach for studying plant viruses. This study evaluated the numbers, diversity, and titer of grapevine viruses in in vitro grapevine plants. The results showed that virus infection status and structure were consistent between in vitro and in vivo plants after long-term shoot tip culture.
Review
Plant Sciences
Carla Benelli, Waed Tarraf, Tolga Izgu, Anna De Carlo
Summary: Plant genetic resources conservation is important for improving agricultural crops, and in vitro conservation is a useful tool for safeguarding plant biodiversity. The slow growth storage (SGS) technique is a valid in vitro approach that can preserve vegetatively propagated species by controlling plantlet growth and development.
Article
Agronomy
Weihong He, Dan Wang, Nan Yang, Dingding Cao, Xiaofeng Chen, Jianjun Chen, Xiangying Wei
Summary: Sesuvium portulacastrum is an important coastal halophyte used for phytoremediation and erosion control. However, traditional propagation methods are not sufficient and may lead to disease spread. This study established an efficient protocol using growth regulators to induce axillary shoots and produce disease-free plants through microcutting and rooting techniques. This provides a rapid and reliable method for the propagation of genetically identical and healthy S. portulacastrum plants.
Review
Biotechnology & Applied Microbiology
Barbara Thiem, Anastasia Aliesa Hermosaningtyas, Anna Budzianowska, Malgorzata Kikowska
Summary: This article summarises the development of an in vitro propagation method for selected medicinal plants by Polish researchers and botanists. The utilization of medicinal plants as a source of raw materials for the pharmaceutical industry has gained significant attention since the 1980s. Medicinal plants have been identified as a potential alternative source for essential compounds with various applications. The plant micropropagation system provides a solution to both the uniform biomass production for bioactive secondary metabolites and the conservation of rare or endangered plant species.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Saikat Gantait, Sangata Das, Manisha Mahanta, Maitreyi Banerjee
Summary: This study for the first time investigated the impact of in vitro culture age on Rauvolfia serpentina, and found that it affected the morphology, antioxidant activities, and reserpine production. The study showed that the shoot length and internode lengths were significantly different among different cultures. The antioxidant activities were reduced in the in vitro culture compared to the ex vitro plantlet, but the reserpine production increased in the in vitro culture. The study also revealed a certain level of genetic variation between the in vitro culture and the ex vitro plantlet.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Agronomy
Julian Sebastian Ramirez Moreno, Sergio Andres Vega Porras, Roosevelt Humberto Escobar, Elena E. Stashenko, Jorge Luis Fuentes Lorenzo, Luz Nayibe Garzon Gutierrez
Summary: This research introduced an efficient micropropagation protocol for Lippia origanoides using sterile seeds and various plant growth regulators. The study found that the high percentage of callus formation and increased shoot number can be achieved by optimizing the concentrations of BAP and NAA. Additionally, the acclimatization rate of the obtained plantlets was high, demonstrating the success of the protocol.
Article
Multidisciplinary Sciences
Rahman Ebrahimzadegan, Asad Maroufi
Summary: Dragon's head plant (Lallemantia iberica) is a flowering species belonging to the mint family. This study developed a simple and efficient method for regeneration and transformation of L. iberica. Cotyledonary nodes showed the best regeneration response, and Agrobacterium-mediated transformation was successfully accomplished. The established methods open doors for further plant improvement, functional analysis, and gene editing applications.
SCIENTIFIC REPORTS
(2022)
Review
Biology
Elena Popova, Irina Kulichenko, Haeng-Hoon Kim
Summary: This article discusses the five major strategies available at the regrowth stage for improving plant material performance after cryopreservation, including physical and chemical factors. These strategies can help choose the most suitable regrowth conditions for plant germplasm to be cryopreserved, and propose combinations of factors that may benefit the recovery of cryopreservation-sensitive species and types of materials.
Article
Biochemistry & Molecular Biology
Karolina Czech, Katarzyna Gawel-Beben, Agnieszka Szopa, Wirginia Kukula-Koch, Thomas Jakschitz, Gunther Bonn, Shah Hussain, Pawel Kubica, Halina Ekiert, Kazimierz Glowniak
Summary: This study compared the phytochemical composition and antioxidant and tyrosinase inhibitory properties of aqueous and hydroethanolic extracts from A. millefolium obtained from field conditions (AmL and AmH extracts) and in vitro cultures (AmIV extracts). The results showed that AmIV extracts had significantly different phytochemical composition compared to AmL and AmH extracts, with fewer polyphenolic compounds and higher fatty acid content. AmIV extracts had lower polyphenol content, weaker antioxidant activity, and no tyrosinase inhibitory properties. Further research is needed before microshoot cultures of A. millefolium can be used as valuable raw material in the cosmetics industry.
Review
Medicine, Research & Experimental
Sara Motyka, Karolina Jafernik, Halina Ekiert, Javad Sharifi-Rad, Daniela Calina, Basem Al-Omari, Agnieszka Szopa, William C. Cho
Summary: The use of plant secondary metabolites, particularly aryltetralin lignan-podophyllotoxin (PTOX), in preventing and treating cancer has gained significant attention. PTOX exhibits potent cytotoxic activity but cannot be used in its pure form due to toxicity and side effects. Efforts are focused on synthesizing PTOX analogs with enhanced therapeutic properties. PTOX derivatives are currently used as anticancer drugs with additional effects such as immunosuppression, antiviral activity, antioxidation, hypolipemia, and anti-inflammation. In vitro cultures of Podophyllum peltatum are highlighted as a valuable source of lignans, including PTOX. Preclinical pharmacological studies confirm the anticancer potential of PTOX and its derivatives, but clinical studies are needed for further validation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Monika Michalak, Martyna Zagorska-Dziok, Marta Klimek-Szczykutowicz, Agnieszka Szopa
Summary: In this study, various drying methods were used to analyze the phenolic profile and biological activity of methanol and water-methanol extracts of dried borage (Borago officinalis) herb. The extracts were found to contain flavonoids and phenolic acids, and the highest content of flavonoids was observed in the methanol extract from the hot-air-dried herb, while the methanol extract from the air-dried herb had the highest content of phenolic acids. In vitro tests demonstrated that the extracts have antioxidant, anti-inflammatory, and anti-ageing properties, making them a valuable source of bioactive compounds for skin cell protection.
Article
Biochemistry & Molecular Biology
Katarzyna Czarnek, Malgorzata Tatarczak-Michalewska, Piotr Dreher, Vishnu D. Rajput, Grzegorz Wojcik, Anna Gierut-Kot, Agnieszka Szopa, Eliza Blicharska
Summary: Metalloenzymes are important for regulating biological functions, and biofortification of plant materials is an effective way to prevent mineral deficiencies in human diets. This study combined biofortification of wheat sprouts with UV-C radiation treatment for seed surface sterilization and found that UV-C radiation effectively suppressed seed germination contamination by microorganisms. The sterilization process did not affect the growth and development of sprouts or nutrient bioassimilation. The results showed that wheat sprouts easily accumulate Fe, Zn, Mg, and Cr during growth, with the optimal concentrations for biofortification being 100 μg g(-1) for Fe and Zn, while the best-developed sprouts were grown in 50 μg g(-1) of Cr solution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Karolina Jafernik, Agata Ladniak, Eliza Blicharska, Katarzyna Czarnek, Halina Ekiert, Agnieszka E. Wiacek, Agnieszka Szopa
Summary: Chitosan-based nanoparticles (chitosan-based nanocomposites; chitosan nanoparticles; ChNPs) are promising materials that have attracted a lot of attention in recent decades. They have great potential as nanocarriers and can encapsulate drugs and active compounds for controlled release. This article provides an overview of the most commonly used preparation methods and their applications in medicine.
Article
Biochemistry & Molecular Biology
Sara Motyka, Barbara Kusznierewicz, Halina Ekiert, Izabela Korona-Glowniak, Agnieszka Szopa
Summary: The purpose of this study was to evaluate the phytochemical profiles of different plant raw materials obtained from Salvia hispanica, and to demonstrate their significant contribution to antioxidant and antimicrobial activities. The study used HPLC-DAD and LC-Q-Orbitrap HRMS techniques to analyze the compounds present in the plant materials. The results showed that the extracts from the leaves exhibited the strongest antibacterial and antifungal activity. This study provides valuable knowledge on the biologically active metabolites present in different parts of Salvia hispanica and their potential applications.
Review
Biochemistry & Molecular Biology
Karolina Jafernik, Halina Ekiert, Agnieszka Szopa
Summary: "A Review of Schisandra henryi: Chemical Composition and Biological Properties" provides a comprehensive review of the research conducted on this endemic plant species in Yunnan, China. The chemical composition of S. henryi is similar to the well-known medicinal plant S. chinensis, and it exhibits various pharmacological properties. The study also highlights the biotechnological potential of S. henryi in in vitro cultures as an alternative source of raw material. This article summarizes the research on the chemical profile and diverse medicinal effects of S. henryi.
Article
Biochemistry & Molecular Biology
Karolina Czech, Katarzyna Gawel-Beben, Agnieszka Szopa, Wirginia Kukula-Koch, Thomas Jakschitz, Gunther Bonn, Shah Hussain, Pawel Kubica, Halina Ekiert, Kazimierz Glowniak
Summary: This study compared the phytochemical composition and antioxidant and tyrosinase inhibitory properties of aqueous and hydroethanolic extracts from A. millefolium obtained from field conditions (AmL and AmH extracts) and in vitro cultures (AmIV extracts). The results showed that AmIV extracts had significantly different phytochemical composition compared to AmL and AmH extracts, with fewer polyphenolic compounds and higher fatty acid content. AmIV extracts had lower polyphenol content, weaker antioxidant activity, and no tyrosinase inhibitory properties. Further research is needed before microshoot cultures of A. millefolium can be used as valuable raw material in the cosmetics industry.
Article
Biotechnology & Applied Microbiology
Wojciech Makowski, Aleksandra Krolicka, Barbara Tokarz, Agnieszka Szopa, Halina Ekiert, Krzysztof Michal Tokarz
Summary: This study investigates the biomass production, synthesis, and productivity of various phenolic acids, flavonoids, and shikonin in Pontechium maculatum. The results show that temporary immersion bioreactors are suitable for the production of phenolic compounds in tissue cultures of P. maculatum.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Food Science & Technology
Sara Motyka, Ewa Skala, Halina Ekiert, Agnieszka Szopa
JOURNAL OF FUNCTIONAL FOODS
(2023)
Review
Pharmacology & Pharmacy
Mario Sousa-Pimenta, Leticia M. Estevinho, Agnieszka Szopa, Mahnoor Basit, Khushbukhat Khan, Muhammad Armaghan, Manshuk Ibrayeva, Eda Sonmez Gurer, Daniela Calina, Christophe Hano, Javad Sharifi-Rad
Summary: Many biological and synthetic agents have been explored to stop the spread of cancer and cure it. Paclitaxel is an effective anticancer drug derived from the tree Taxus brevifolia, and its derivatives have been established as therapeutic compounds against neoplastic disorders. This review explores the mechanisms of action of paclitaxel and its derivatives, different formulations available, as well as the role of paclitaxel in hematological malignancies and potential limitations in its clinical use. The impact of paclitaxel on other oncogenic pathways and its immunomodulatory potential are also analyzed, providing insights for future chemotherapeutic approaches to cancer.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Anna Budzianowska, Monika Derda, Jaromir Budzianowski, Agnieszka Szopa, Malgorzata Kikowska
Summary: The aim of this study was to compare the potency of Plantago media L. extracts on Acanthamoeba sp. trophozoites. The extracts from leaves showed the strongest anti-amoebic effect, and the presence of phenylethanoid glycoside-acteoside was responsible for the activity. This is the first report on the amoebicidal activity of Plantago media extracts produced by biotechnological methods.
APPLIED SCIENCES-BASEL
(2023)
Review
Pharmacology & Pharmacy
Marta Sharafan, Magdalena A. Malinowska, Halina Ekiert, Beata Kwasniak, Elzbieta Sikora, Agnieszka Szopa
Summary: This review provides an overview of the botanical, ecological, and phytochemical characteristics of Vitis vinifera L., as well as its applications in the food industry, medicine, and phytocosmetology. The focus is on the cosmetic properties of V. vinifera, including its anti-ageing, anti-inflammatory, and skin-whitening effects. The review also highlights the importance of biotechnological studies on V. vinifera and discusses its safety for use.
Article
Biochemistry & Molecular Biology
Ewelina Sobstyl, Agnieszka Szopa, Malgorzata Olszowy-Tomczyk, Sebastian Gnat, Karolina Jafernik, Irena Maria Choma
Summary: HPLC and TLC profiling were conducted on leaf and fruit extracts of five species from the Schisandraceae family. Lignans and phenolic compounds were found in both fruits and leaves of all species. The highest abundance of lignans was detected in the fruit extract of Schisandra chinensis, with 15 compounds identified. The fruit extracts of S. chinensis and S. rubriflora exhibited exceptional antibacterial activity, while all fruit and leaf extracts showed enzyme-inhibiting abilities, except for the fruit extract of Kadsura japonica. Antioxidant activity was stronger in the leaf extracts compared to the fruit extracts, assessed using TLC-direct bioautography and spectrophotometric measurements.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Biochemistry & Molecular Biology
Marta Sharafan, Magdalena Anna Malinowska, Marta Kubicz, Pawel Kubica, Marin-Pierre Gemin, Cecile Abdallah, Manon Ferrier, Christophe Hano, Nathalie Giglioli-Guivarc'h, Elzbieta Sikora, Arnaud Lanoue, Agnieszka Szopa
Summary: The primary purpose of this study was to initiate and optimize shoot cultures of different Vitis vinifera L. cultivars and investigate the influence of media and genotype on metabolic content. The use of MS media variants induced the production of certain metabolites and their antioxidant and anti-tyrosinase activities were measured. The findings suggest that in vitro cultured grapes can be a potential alternative source of plant material for the cosmetic industry.
Article
Medicine, General & Internal
Agnieszka Szopa, Sara Motyka, Halina Ekeirt
Summary: Plant sprouts, including chia sprouts and microgreens, have gained attention due to their rich chemical composition and potential health benefits. This review article explores the research on chia sprouts and microgreens, highlighting their enhanced nutritional properties and potential antimicrobial effects. The study also reveals the positive impact of sprouting on antioxidant potential, increasing the production of polyphenolic compounds.
CURRENT ISSUES IN PHARMACY AND MEDICAL SCIENCES
(2023)
