Article
Multidisciplinary Sciences
Sabine Ruegenberg, Felix A. M. C. Mayr, Ilian Atanassov, Ulrich Baumann, Martin S. Denzel
Summary: The hexosamine pathway (HP) is a key anabolic pathway that regulates the lifespan of Caenorhabditis elegans. Protein kinase A (PKA) mediates the phosphorylation of GFAT-1 to control its activity and feedback inhibition mechanism.
NATURE COMMUNICATIONS
(2021)
Article
Fisheries
Muzi Zhang, Shidong Wang, Liying Sun, Lei Gan, Yanhong Lin, Jian Shao, Haibo Jiang, Ming Li
Summary: Fishes can adapt to environmental ammonia, and the study found that the CPS I mRNA was significantly up-regulated in liver and kidney tissues after acute ammonia stress. The RNA interference of CPS I led to changes in enzyme activities related to urea cycle and glutamine synthesis, suggesting a shift in ammonia detoxification strategies.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Yue Zhang, Jiang Li, Yixian Huang, Yuang Chen, Zhangyi Luo, Haozhe Huang, Raymond E. West III, Thomas D. Nolin, Zhou Wang, Song Li
Summary: Inhibition of Myc and GFAT-1 in combination can effectively inhibit the proliferation and migration of prostate cancer cells and improve tumor immune microenvironment. The development of a prodrug conjugate loaded into a nanocarrier enhances the delivery of the inhibitors, providing a potential strategy for the treatment of prostate cancer.
Article
Biochemistry & Molecular Biology
Lionel Ballut, Sebastien Violot, Frederic Galisson, Isabelle R. Goncalves, Juliette Martin, Santosh Shivakumaraswamy, Loic Carrique, Hemalatha Balaram, Nushin Aghajari
Summary: This study investigated the structure and catalytic mechanism of glutamine amidotransferases. Crystal structure analysis revealed that domain rotation is associated with catalysis and a previously overlooked signature motif related to catalysis was identified.
Article
Chemistry, Multidisciplinary
Carla Calvo-Tusell, Miguel A. Maria-Solano, Silvia Osuna, Ferran Feixas
Summary: This research investigates the allosteric activation mechanisms of IGPS using molecular dynamics simulations, enhanced sampling techniques, and dynamical networks. The simulations reveal how IGPS captures the substrate, forms a closed interface, and creates an oxyanion hole for efficient substrate hydrolysis under the influence of the allosteric effector. The study provides insights into the molecular details of allosteric activation and can guide engineering efforts of IGPS.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Isadora A. Oliveira, Diego Allonso, Tacio V. A. Fernandes, Daniela M. S. Lucena, Gustavo T. Ventura, Wagner Barbosa Dias, Ronaldo S. Mohana-Borges, Pedro G. Pascutti, Adriane R. Todeschini
Summary: Glycoconjugates play a key role in cellular processes and changes in their composition are linked to various human pathologies. This study successfully expressed and purified catalytically active recombinant hGFAT2, revealing unique enzymatic properties and structural features.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Hyungjoo Shon, Elena A. Matveeva, Ella C. Jull, Yijia Hu, Tiffany A. Coupet, Young-Sam Lee
Summary: This study provides time-course mass spectrometric data supporting the channeling of CAIR between domains of recombinant human PAICS, indicating that the SAICAR synthase domain selectively uses enzyme-made CAIR. This channeling may be a part of a larger channeling process in de novo purine nucleotide synthesis.
Article
Multidisciplinary Sciences
Xianhui Liu, Ivana Blazenovic, Adam J. Contreras, Thu M. Pham, Christine A. Tabuloc, Ying H. Li, Jian Ji, Oliver Fiehn, Joanna C. Chiu
Summary: The integration of circadian and metabolic signals is crucial for maintaining robust circadian rhythms and efficient metabolism. Nutrient input is a key factor influencing the rhythm of protein O-GlcNAcylation, a nutrient-sensitive post-translational modification.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Lionel Ballut, Sebastien Violot, Sanjeev Kumar, Nushin Aghajari, Hemalatha Balaram
Summary: This review presents the current understanding of the molecular basis of catalysis in GMPS, providing the first comprehensive assessment of the biochemical function of this intriguing enzyme.
Article
Chemistry, Physical
Zhikun Tong, Xiang Li, Jingyu Dong, Rui Gao, Qiang Deng, Jun Wang, Zheling Zeng, Ji-Jun Zou, Shuguang Deng
Summary: Developing a catalyst to upgrade furfural to alcohols is important for fine chemical synthesis. Metal phosphate nanoparticles with different compositions were synthesized, showing high catalytic efficiency for producing alcohols. The catalysts displayed high activity and stability during recycling experiments.
Article
Agronomy
Emanuele La Bella, Andrea Baglieri, Ermes Ivan Rovetto, Piergiorgio Stevanato, Ivana Puglisi
Summary: The study investigated the application of Chlorella vulgaris extract on lettuce seedlings, which positively influenced the growth of seedlings, increased chlorophylls, carotenoids, protein content, and ashes in shoots. Additionally, the extract also had a positive impact on the nitrogen metabolism in shoots and increased dry matter, proteins, and ash content in roots.
Article
Biotechnology & Applied Microbiology
Ivana Puglisi, Emanuele La Bella, Ermes Ivan Rovetto, Piergiorgio Stevanato, Giancarlo Fascella, Andrea Baglieri
Summary: Microalgae-based products applied as biostimulants have attracted the attention of researchers. This study evaluated the effect of two different methods of Chlorella vulgaris extract application (foliar spray and root drenching) on lettuce seedlings. The growth parameters, chlorophyll, carotenoid, and protein contents, as well as enzymatic activities involved in primary and secondary metabolism, were monitored. The results showed that both application methods positively influenced the growth and biochemical response of lettuce seedlings, although their mechanisms of action seemed to be different.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Plant Sciences
Kumar Lama, Li-Juan Chai, Reut Peer, Huiqin Ma, Yelena Yeselson, Arthur A. Schaffer, Moshe A. Flaishman
Summary: Female fig (Ficus carica L.) fruits show a major increase in volume and sugar content during the final week of development, with a temporal separation between water content increase and subsequent sugar content increase. Figs are an extreme example of sugar import and accumulation, with import rates significantly higher than other sugar-accumulating fruit species. By comparing female and male fig fruits, specific genes related to sugar accumulation were highlighted, indicating a global transition in sugar metabolism and transport gene expression during this period.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Biochemistry & Molecular Biology
Sachin Dhawale, Madhuri Pandit, Kanchan Thete, Dnyaneshwari Ighe, Sachin Gawale, Pallavi Bhosle, Deepak K. Lokwani
Summary: Candida albicans is a common fungus that can cause life-threatening infections and high mortality rates. In this study, an in silico approach was used to analyze the inhibitory potential of polyphenols from plants against a crucial enzyme in the cell wall of C. albicans.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biochemistry & Molecular Biology
Joanna Stefaniak, Michal G. Nowak, Marek Wojciechowski, Slawomir Milewski, Andrzej S. Skwarecki
Summary: Glucosamine-6-phosphate synthase (GlcN-6-P synthase) is an important target for the development of antimicrobial agents and antidiabetic drugs. Various compounds have been identified as inhibitors of this enzyme, but their antimicrobial activity is relatively low and they have poor penetration ability. The pro-drug approach has been used to improve the antimicrobial activity of these inhibitors. Additionally, heterocyclic and polycyclic compounds have shown antimicrobial activity and potential as inhibitors of the enzyme, pending confirmation of their selective toxicity.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Miriam Benito-Cambra, Pierre Gareil, Bernard Badet, Marie-Ange Badet-Denisot, Nathalie Delaunay
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2018)
Article
Cell Biology
Asa Birna Birgisdottir, Stephane Mouilleron, Zambarlal Bhujabal, Martina Wirth, Eva Sjottem, Gry Evjen, Wenxin Zhang, Rebecca Lee, Nicola O'Reilly, Sharon A. Tooze, Trond Lamark, Terje Johansen
Article
Multidisciplinary Sciences
M. Teresa Bertran, Stephane Mouilleron, Yanxiang Zhou, Rakhi Bajaj, Federico Uliana, Ganesan Senthil Kumar, Audrey van Drogen, Rebecca Lee, Jennifer J. Banerjee, Simon Hauri, Nicola O'Reilly, Matthias Gstaiger, Rebecca Page, Wolfgang Peti, Nicolas Tapon
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Martina Wirth, Wenxin Zhang, Minoo Razi, Lynet Nyoni, Dhira Joshi, Nicola O'Reilly, Terje Johansen, Sharon A. Tooze, Stephane Mouilleron
NATURE COMMUNICATIONS
(2019)
Article
Cell Biology
Jessica Diring, Stephane Mouilleron, Neil Q. McDonald, Richard Treisman
NATURE CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Clement Cabriel, Nicolas Bourg, Pierre Jouchet, Guillaume Dupuis, Christophe Leterrier, Aurelie Baron, Marie-Ange Badet-Denisot, Boris Vauzeilles, Emmanuel Fort, Sandrine Leveque-Fort
NATURE COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Jingjing Zhou, Ludovic Pecqueur, Agota Aucynaite, Jonathan Fuchs, Rasa Rutkiene, Justas Vaitekunas, Rolandas Meskys, Matthias Boll, Marc Fontecave, Jaunius Urbonavicius, Beatrice Golinelli-Pimpaneau
Summary: This study reports the crystal structure of a [Fe-S]-containing protein called TudS and its mechanism in the desulfuration of thiouracil. The [4Fe-5S] state of the cluster was identified as a catalytic intermediate, with a water molecule and catalytically important residues Ser101 and Glu45 playing key roles in the reaction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biology
Roman O. Fedoryshchak, Magdalena Prechova, Abbey M. Butler, Rebecca Lee, Nicola O'Reilly, Helen R. Flynn, Ambrosius P. Snijders, Noreen Eder, Sila Ultanir, Stephane Mouilleron, Richard Treisman
Article
Biochemistry & Molecular Biology
Ornella Bimai, Simon Arragain, Beatrice Golinelli-Pimpaneau
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Zhou Jingjing, Lenon Marine, Ravanat Jean-Luc, Nadia Touati, Velours Christophe, Podskoczyj Karolina, Leszczynska Grazyna, Fontecave Marc, Barras Frederic, Golinelli-Pimpaneau Beatrice
Summary: The research findings showed that MnmA from Escherichia coli can bind a [4Fe-4S] cluster for tRNA sulfuration, maintaining the accuracy of protein translation. Additionally, the study revealed that E. coli cells retain s(2)U34 biosynthesis even in the absence of functional ISC and SUF [Fe-S] cluster assembly machineries.
NUCLEIC ACIDS RESEARCH
(2021)
Correction
Biochemistry & Molecular Biology
Jingjing Zhou, Marine Lenon, Jean-Luc Ravanat, Nadia Touati, Christophe Velours, Karolina Podskoczyj, Grazyna Leszczynska, Marc Fontecave, Frederic Barras, Beatrice Golinelli-Pimpaneau
NUCLEIC ACIDS RESEARCH
(2021)
Editorial Material
Cell Biology
Yakubu Princely Abudu, Stephane Mouilleron, Sharon A. Tooze, Trond Lamark, Terje Johansen
Summary: Research has identified SAMM50 as a receptor for basal mitophagy, mediating the process through interaction with Atg8-family proteins and SQSTM1/p62. During a metabolic switch to oxidative phosphorylation, SAMM50 cooperates with SQSTM1 to facilitate efficient basal mitophagy.
Article
Biochemistry & Molecular Biology
Christophe Velours, Jingjing Zhou, Paolo Zecchin, Nisha He, Myriam Salameh, Marie-Pierre Golinelli-Cohen, Beatrice Golinelli-Pimpaneau
Summary: Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS) is a technique used to determine the absolute molar mass of macromolecules in solution by detecting their light scattering intensity. This technique can be applied to proteins and polymers of any shape without relying on assumptions or calibrations. However, corrections are needed for samples that absorb light at the MALS laser wavelength. In this study, various corrections were applied to determine the absolute molar mass of [2Fe-2S] and [4Fe-4S] cluster-containing proteins in both apo- and holo-forms. The determination of the absolute molar mass of the [2Fe-2S]-containing holo-NEET proteins revealed changes in oligomerization state upon cluster binding, highlighting an essential function of the cluster.
Article
Chemistry, Inorganic & Nuclear
Beatrice Golinelli-Pimpaneau
Summary: AlphaFold and RoseTTAFold have successfully predicted protein structures, including the iron-sulfur binding sites. However, experimental confirmation is still needed to fully understand the coordination state and conformational changes during catalysis.
Article
Biochemistry & Molecular Biology
Beatriz Gomes Guimaraes, Beatrice Golinelli-Pimpaneau
Summary: The study demonstrates the successful application of the sulfur-SAD method to determine the structure of human dihydrouridine synthase's double stranded RNA binding domain with highly redundant data. It suggests that the sulfur-SAD phasing approach should be considered for proteins with average sulfur content diffracting better than 3 angstrom resolution.
CURRENT RESEARCH IN STRUCTURAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)