Article
Multidisciplinary Sciences
Jessica L. Caldwell, I-Ju Lee, Lena Ngo, Lianguo Wang, Sherif Bahriz, Bing Xu, Donald M. Bers, Manuel F. Navedo, Julie Bossuyt, Yang K. Xiang, Crystal M. Ripplinger
Summary: This study investigated the role of cAMP signaling in sex-dependent functional responses in the heart. The researchers found that cAMP activation was uniform in male hearts, but decayed faster in apical regions of female hearts, leading to uneven action potential changes and alterations in repolarization direction.
Article
Cell Biology
Shailesh R. Agarwal, Rinzhin T. Sherpa, Karni S. Moshal, Robert D. Harvey
Summary: Activation of different receptors can lead to distinct functional responses in cardiac myocytes through the generation of cAMP. The compartmentation of cAMP signaling involves localized production, breakdown, and diffusion limitations, which play a crucial role in generating receptor-specific responses. Technological advancements have provided critical breakthroughs in understanding these mechanisms, yet gaps still exist in our knowledge of cAMP compartmentation in cardiac myocytes.
CELLULAR SIGNALLING
(2022)
Article
Pharmacology & Pharmacy
Dmytro Isaev, Waheed Shabbir, Ege Y. Dinc, Dietrich E. Lorke, Georg Petroianu, Murat Oz
Summary: CBD affects ion channels in rabbit ventricular myocytes, including inhibiting the activity of voltage-gated Na+ and Ca2+ channels, as well as reducing ion currents mediated by rapidly and slowly activated delayed rectifier K+ channels. These findings suggest caution should be exercised when using CBD, especially for individuals with cardiac channelopathies or using drugs that affect heart rhythm or contractility.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Kirin D. Gada, Mengmeng Chang, Aishwarya Chandrashekar, Leigh D. Plant, Sami F. Noujaim, Diomedes E. Logothetis
Summary: G-protein-gated inwardly rectifying potassium (GIRK) channel activity is regulated by phosphatidylinositol-4,5-bisphosphate (PI 4,5P(2)), and PKC epsilon plays a role in the activation of GIRK channels in cardiac atrial cells and human stem cell-derived atrial cardiomyocytes. PKC epsilon enhances the interaction between GIRK4 and GIRK1/4 with PIP2, leading to increased channel activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Po Wei Kang, Nourdine Chakouri, Johanna Diaz, Gordon F. Tomaselli, David T. Yue, Manu Ben-Johny
Summary: The action of calmodulin on NaV1.5 channels is crucial for the occurrence of arrhythmias. Disruption of Ca2+-free CaM preassociation with NaV1.5 results in a decrease in peak open probability and an increase in persistent NaV openings, altering the inactivation mechanism of NaV1.5.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Plant Sciences
Neelesh Patra, Shruthi Hariharan, Hena Gain, Mrinal K. Maiti, Arpita Das, Joydeep Banerjee
Summary: Plant growth, development, and crop productivity are influenced by the interaction of plants with various abiotic and biotic factors, with calcium ion playing a crucial role in modulating responses to stress. Calcium sensor proteins are key in coupling stress stimuli with appropriate responses in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Michael Levin
Summary: Recent advancements in analyzing developmental bioelectric circuits and channelopathies have shed light on the cooperation of cellular collectives towards achieving organ-level structural order. These progressions open up possibilities for utilizing bioelectric signaling in interventions related to developmental disorders, regenerative medicine, cancer reprogramming, and synthetic bioengineering.
Review
Cell Biology
Camille E. Blandin, Basile J. Gravez, Stephane N. Hatem, Elise Balse
Summary: Both inherited and acquired cardiac arrhythmias are often linked to abnormal functional expression of ion channels at the cellular level, with mutations in genes encoding ion channels being a major factor in inherited cases. Disruption of normal channel trafficking due to factors like fibrosis and altered myocyte contacts is common in acquired cardiac arrhythmias. Channel availability, particularly in hERG and K(V)1.5 channels, may be another significant arrhythmogenic mechanism. Knowledge of these molecular mechanisms could lead to novel antiarrhythmic strategies.
Article
Cardiac & Cardiovascular Systems
Dandan Yang, Xiaoping Wan, Adrienne T. Dennis, Emre Bektik, Zhihua Wang, Mauricio G. S. Costa, Charline Fagnen, Catherine Venien-Bryan, Xianyao Xu, Daniel H. Gratz, Thomas J. Hund, Peter J. Mohler, Kenneth R. Laurita, Isabelle Deschenes, Ji-Dong Fu
Summary: This study reveals a novel biophysical action of endogenous miRs in modulating cardiac electrophysiology through noncanonical mechanisms. Endogenous miR1 physically binds with cardiac membrane protein Kir2.1, suppressing I-K1 and affecting action potential of cardiomyocytes. The findings suggest that miRs may be involved in the pathogenesis of cardiac arrhythmias by regulating ion channel physiology and pathology.
Review
Biochemistry & Molecular Biology
Filip Benko, Dana Urminska, Michal Duracka, Eva Tvrda
Summary: This review provides an overview of specific ion channels and transporters located in the mammalian sperm plasma membrane and their functional roles in sperm development and function. It discusses the potential therapeutic targets for infertility management.
Editorial Material
Plant Sciences
Yunjing Wang, Qian Gong, Zhenhui Jin, Yule Liu, Yiguo Hong
Summary: A new study reveals the genetic link between calcium signaling and RNA interference (RNAi) in plants, showing that wound-triggered calcium flux serves as an initial messenger for priming RNAi for its role in plant antiviral defense. This discovery provides insights into plant development and response to various stresses.
TRENDS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Michael Arzt, Marzena A. Drzymalski, Sarah Ripfel, Sebastian Meindl, Alexander Biedermann, Melanie Durczok, Karoline Keller, Julian Mustroph, Sylvia Katz, Maria Tafelmeier, Simon Lebek, Bernhard Floerchinger, Daniele Camboni, Sigrid Wittmann, Johannes Backs, Christof Schmid, Lars S. Maier, Stefan Wagner
Summary: Patients with sleep-disordered breathing (SDB) have increased oxidized and activated CaMKII levels and CaMKII-dependent Ca spark frequency in the atrial myocardium, independent of major clinical confounders.
Article
Cardiac & Cardiovascular Systems
Marta Perez-Hernandez, Alejandra Leo-Macias, Sarah Keegan, Mariam Jouni, Joon-Chul Kim, Esperanza Agullo-Pascual, Sarah Vermij, Mingliang Zhang, Feng-Xia Liang, Paul Burridge, David Fenyo, Eli Rothenberg, Mario Delmar
Summary: This study identified a subcellular domain formed by sodium channel clusters and subjacent subsarcolemmal mitochondria in adult cardiac myocytes, shedding light on the intersection between electrical and structural functions of the heart. The proximity of Na(V)1.5 channels to mitochondria resulted in functional changes in mitochondria upon TTX exposure, with increased Ca2+ accumulation and reactive oxygen species production. Additionally, a negative correlation between SCN5A and SLC8B1 at a transcriptional level was observed, highlighting the intricate crosstalk between sodium channels and mitochondria.
CIRCULATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Yu Yuan, Dawid Jaslan, Taufiq Rahman, Stephen R. Bolsover, Vikas Arige, Larry E. Wagner, Carla Abrahamian, Rachel Tang, Marco Keller, Jonas Hartmann, Anna S. Rosato, Eva-Maria Weiden, Franz Bracher, David Yule, Christian Grimm, Sandip Patel
Summary: This study reveals that TPC2 can selectively increase its permeability to calcium ions when co-activated by the endogenous ligands NAADP and PI(3,5)P-2. This finding indicates that the flux of different ions through the same channel can be independently controlled and identifies TPC2 as a potential coincidence detector that optimizes lysosomal calcium signaling.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Kristyna Vydra Bousova, Monika Zouharova, Katerina Jiraskova, Veronika Vetyskova
Summary: This article focuses on the interaction between TRPM channels and CaM, and summarizes the effects of CaM interactions and modulations on TRPM channels in cellular physiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Donald M. Bers, Stanley Nattel
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Multidisciplinary Sciences
Yankun Lyu, Phung N. Thai, Lu Ren, Valeriy Timofeyev, Zhong Jian, Seojin Park, Kenneth S. Ginsburg, James Overton, Julie Bossuyt, Donald M. Bers, Ebenezer N. Yamoah, Ye Chen-Izu, Nipavan Chiamvimonvat, Xiao-Dong Zhang
Summary: This study investigates the regulation of pH(i) in cardiomyocytes and finds a correlation between cardiac contractions and pH(i) transients. The pH(i) transients are influenced by various factors, including pacing rate, transporters, buffering capacity, and β-adrenergic signaling. Mitochondrial activity plays a role in pH(i) regulation, and a decrease in pH(i) may act as a negative feedback to cardiac contractions.
Letter
Cardiac & Cardiovascular Systems
Bence Hegyi, Juliana Mira Hernandez, Erin Y. Shen, Nima R. Habibi, Julie Bossuyt, Donald M. Bers
Article
Biophysics
Kyoko Yoshida, Jeffrey J. Saucerman, Jeffrey W. Holmes
Summary: Pregnancy represents the intersection of mechanics and biology, with significant changes in mechanical and hormonal cues. This study develops a multiscale cardiac growth model to understand how these cues interact to drive heart growth during pregnancy. The rise in progesterone (P4) during the first half of gestation is identified as an important contributor to heart growth.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Article
Neurosciences
Chidera C. Alim, Christopher Y. Ko, Juliana Mira Hernandez, Erin Y. Shen, Sonya Baidar, Ye Chen-Izu, Donald M. Bers, Julie Bossuyt
Summary: Cardiac mechanical afterload induces autoregulatory increase in myocyte Ca2+ dynamics and contractility, and this process is regulated by calcium/calmodulin-dependent protein kinase II (CaMKII) and nitric oxide (NO).
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Cardiac & Cardiovascular Systems
Julie Bossuyt, Johanna M. Borst, Marie Verberckmoes, Logan R. J. Bailey, Donald M. Bers, Bence Hegyi
Summary: PKD1 plays an important role in the hypertrophic response and downregulation of K+ channels in HF. Inhibition of PKD1 may represent a therapeutic strategy to reduce hypertrophy and arrhythmias, but it may not prevent disease progression and reduced contractility in HF.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Bence Hegyi, Donald M. Bers
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Bence Hegyi, Juliana Mira Hernandez, Christopher Y. Ko, Junyoung Hong, Erin Y. Shen, Emily R. Spencer, Daria Smoliarchuk, Manuel F. Navedo, Donald M. Bers, Julie Bossuyt
Summary: This study developed a novel animal model of HFpEF by combining leptin receptor-deficient db/db mice with aldosterone infusion, which recapitulated the complex human phenotype and multiorgan impairments of HFpEF. This model can be useful for future therapeutic testing and provide unique opportunities to better understand disease pathobiology.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Multidisciplinary Sciences
Jessica L. Caldwell, I-Ju Lee, Lena Ngo, Lianguo Wang, Sherif Bahriz, Bing Xu, Donald M. Bers, Manuel F. Navedo, Julie Bossuyt, Yang K. Xiang, Crystal M. Ripplinger
Summary: This study investigated the role of cAMP signaling in sex-dependent functional responses in the heart. The researchers found that cAMP activation was uniform in male hearts, but decayed faster in apical regions of female hearts, leading to uneven action potential changes and alterations in repolarization direction.
Article
Neurosciences
Eleonora Grandi, Manuel F. Navedo, Jeffrey J. Saucerman, Donald M. Bers, Nipavan Chiamvimonvat, Rose E. Dixon, Dobromir Dobrev, Ana M. Gomez, Osama F. Harraz, Bence Hegyi, David K. Jones, Trine Krogh-Madsen, Walter Lee Murfee, Matthew A. Nystoriak, Nikki G. Posnack, Crystal M. Ripplinger, Rengasayee Veeraraghavan, Seth Weinberg
Summary: This white paper summarises the outcomes of the seventh UC Davis Cardiovascular Research Symposium on Systems Approach to Understanding Cardiovascular Disease and Arrhythmia. The Symposium aimed to bring together experts in cardiovascular biomedicine to focus on the diversity and signaling involved in cardiovascular function. The paper presents an overview of major cell types, explores the complexity of cardiovascular development and disease at various levels, and discusses technological innovations for advancing understanding of integrated cardiovascular function and dysfunction.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Neurosciences
Matthew W. Van de Graaf, Taylor G. Eggertsen, Angela C. Zeigler, Philip M. Tan, Jeffrey J. Saucerman
Summary: Protein interaction databases are important resources for network bioinformatics and integration of molecular experimental data. This study benchmarked five protein interaction databases for their ability to recover manually curated edges from logic-based network models, and found that Pathway Commons performed best. The study also revealed that protein interaction databases have better performance in recovering central, conserved pathways, but have limitations in recovering tissue-specific and transcriptional regulation. The study also identified new edges that improve model predictions, providing new insights into cardiac hypertrophy signaling.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Multidisciplinary Sciences
Anders R. Nelson, Darrian Bugg, Jennifer Davis, Jeffrey J. Saucerman
Summary: The RNA-binding protein muscleblind-like1 (MBNL1) has been identified as a key regulator of cardiac wound healing and myofibroblast activation. Through integrating genome-wide screens and a fibroblast network model, this study identified 14 potential MBNL1 targets and developed novel signaling modules for various pathways. Experimental validation confirmed MBNL1 regulation of p38 expression in mouse cardiac fibroblasts. The expanded fibroblast model predicted a hierarchy of MBNL1-regulated pathways with a strong influence on aSMA expression. This study lays the foundation for exploring the network mechanisms of MBNL1 signaling in fibrosis.
Article
Cardiac & Cardiovascular Systems
Jakub Tomek, Madeline Nieves-Cintron, Manuel F. Navedo, Christopher Y. Ko, Donald M. Bers
Summary: SparkMaster 2 is a new and user-friendly software tool for accurate high-throughput analysis of line-scan Ca spark imaging data. It offers high accuracy, ease of use, and valuable built-in features to facilitate visualization, analysis, and interpretation of Ca spark and wave data, enhancing the quality and throughput of research on arrhythmogenic Ca release events in cardiomyocytes.
CIRCULATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Bengt Svensson, Florentin R. Nitu, Robyn T. Rebbeck, Lindsey M. Mcgurran, Tetsuro Oda, David D. Thomas, Donald M. Bers, Razvan L. Cornea
Summary: Current studies have shown that Ca2+ leakage from cardiomyocyte sarcoplasmic reticulum through hyperactive resting cardiac ryanodine receptor channels (RyR2) has a pro-arrhythmic effect. An exogenous peptide (DPc10) binding promotes leaky RyR2 in cardiomyocytes and reports on the endogenous state. Conversely, binding of calmodulin (CaM) inhibits RyR2 leak and low CaM affinity is indicative of leaky RyR2. These findings have led to the development of a FRET biosensor for drug discovery targeting RyR2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Thomas R. Shannon, Dan J. Bare, Sabine Van Dijk, Shayan Raofi, Tiffany N-M Huynh, Yang K. Xiang, Julie Bossuyt, Kimberly L. Dodge-Kafka, Kenneth S. Ginsburg, Donald M. Bers
Summary: Beta-adrenergic receptor signaling in cardiac myocytes is important for cardiac function, and the spatiotemporal activation of this signaling is still not well understood. Research has shown that localized activation of beta-adrenergic receptors can trigger the spread of calcium transport and uptake in myocytes. Intracellular mobility of beta-adrenergic receptors and AKAP7 gamma may play a role in this activation spread.
Article
Cardiac & Cardiovascular Systems
Andrew B. Harvey, Renelyn A. Woltes, Raymond N. Deepe, Hannah G. Tarolli, Jenna R. Drummond, Allison Trouten, Auva Zandi, Jeremy L. Barth, Rupak Mukherjee, Martin J. Romeo, Silvia G. Vaena, Ge Tao, Robin Muise-Helmericks, Paula S. Ramos, Russell A. Norris, Andy Wessels
Summary: This study highlights the importance of SOX9 in the regulation of epicardial cell invasion and emphasizes the role of EPDCs in regulating atrioventricular valve development and homeostasis. It also reports a novel expression profile of Cd109, a gene with previously unknown relevance in heart development.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Review
Cardiac & Cardiovascular Systems
MariaSanta C. Mangione, Jinhua Wen, Dian J. Cao
Summary: mTOR, a mechanistic target of rapamycin, is an evolutionarily conserved pathway that plays a fundamental role in nutrient sensing, growth, metabolism, lifespan, and aging. Recent studies have highlighted the regulatory role of mTOR in innate immune responses and its involvement in the pathogenesis of cardiovascular diseases, especially in acute inflammation and atherosclerotic cardiovascular disease. This review also discusses mTOR's role in trained immunity, immune senescence, and clonal hematopoiesis, as well as its architecture and regulatory complexes.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Junlin Li, Yajun Gong, Yiren Wang, Huihui Huang, Huan Du, Lianying Cheng, Cui Ma, Yongxiang Cai, Hukui Han, Jianhong Tao, Gang Li, Panke Cheng
Summary: Myocardial ischemia-reperfusion injury is closely related to the final infarct size in acute myocardial infarction. Regulatory T cells play an important role in the inflammatory response after AMI, but different subtypes of Tregs have different effects on the injury.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Yuxin Chu, Yutao Hua, Lihao He, Jin He, Yunxi Chen, Jing Yang, Ismail Mahmoud, Fanfang Zeng, Xiaochang Zeng, Gloria A. Benavides, Victor M. Darley-Usmar, Martin E. Young, Scott W. Ballinger, Sumanth D. Prabhu, Cheng Zhang, Min Xie
Summary: This study demonstrates that administering beta-hydroxybutyrate (beta-OHB) at the time of reperfusion can reduce infarct size and preserve cardiac function by activating autophagy and preserving mitochondrial homeostasis, potentially through mTOR inhibition.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)