Article
Chemistry, Applied
Philipp Eyring, Marc Tienstra, Hans Mol, Susan Strange Herrmann, Peter Have Rasmussen, Henrik Lauritz Frandsen, Mette Erecius Poulsen
Summary: Various generic extraction methods have been used for determination of pesticide residues, mycotoxins, and PAHs in food and animal feed, but a new method introduced in this study facilitated identification of a wide range of compounds across different polarities. The method involved initial extraction with water, acetonitrile, formic acid, and heptane followed by solid-phase extraction clean-up and analysis by chromatography and mass spectrometry, achieving analyte recoveries with high accuracy and precision.
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Plant Sciences
Ting-Zhi Liao, Yu-Hsuan Chen, Jyh-Nong Tsai, Chieh Chao, Tzu-Pi Huang, Cheng-Fang Hong, Zong-Chi Wu, Isheng Jason Tsai, Hsin-Han Lee, Ned B. Klopfenstein, Mee-Sook Kim, Jane E. Stewart, Ndeme Atibalentja, Fred E. Brooks, Philip G. Cannon, A. Mohd Farid, Tsutomu Hattori, Hoi-Shan Kwan, Regent Yau Ching Lam, Yuko Ota, Norio Sahashi, Robert L. Schlub, Louise S. Shuey, Alvin M. C. Tang, Chia-Lin Chung
Summary: This study investigated the control efficacies and systemic activities of fungicides with different modes of action on brown root rot disease caused by Phellinus noxius. The results showed that three fungicides had high inhibitory effects on the colony growth of P. noxius, and one fungicide also showed good inhibitory effects. In addition, the experiments demonstrated that these fungicides had efficient acropetal translocation after treatment of the root organ.
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Natalja P. Norskov, Annette Bruhn, Andrew Cole, Mette Olaf Nielsen
Summary: This study investigated the metabolic profiles of various seaweeds and hemp, revealing potential compounds with anti-methanogenic properties. Specific seaweeds were found to contain halomethanes, while hemp was rich in lignans and flavonoid glucosides. The findings suggest promising avenues for reducing greenhouse gas emissions in the dairy/beef industry.
Review
Medical Laboratory Technology
Stephanie Rankin-Turner, Liam M. Heaney
Summary: Mass spectrometry (MS) has become a powerful and versatile tool in clinical laboratory, with advancements in biomarker detection, protein measurement, therapeutic drug monitoring, and automation leading to potential revolution in clinical testing.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Chemistry, Analytical
Maartje Verhoeven, Jennifer Bonetti, Ruben Kranenburg, Arian van Asten
Summary: Due to the increasing number of NPSs, identifying drug isomers is becoming more challenging for forensic laboratories. Currently, GC-MS is the standard technique, but it cannot confidently differentiate positional isomers. This review provides an overview of methods for differentiating and identifying NPS positional isomers and proposes a workflow for routine analysis in forensic laboratories.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lauren M. Bishop, Tong Shen, Oliver Fiehn
Summary: Accurate and reproducible quantification of endogenous lipids is crucial for large-scale lipidomic analyses. Many lipids in liquid chromatography-electrospray ionization-mass spectrometry form various adducts with buffer components, and the mechanisms and conditions affecting adduct formation are poorly understood. Variations in adduct ratios can lead to significant inaccuracies during quantification, and the use of multiple adduct ions improves accuracy compared to using a single adduct ion.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Nerea Lorenzo-Parodi, Erich Leitner, Torsten C. Schmidt
Summary: This study compares three gas chromatographic methods for analyzing ten iodinated derivatives of aromatic amines, and finds that all methods and most analytes have good detection limits and precision. Urine samples from smokers and non-smokers were successfully analyzed, and differences in concentration were observed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Baoyi Wang, Fengfeng Qu, Peiqiang Wang, Lei Zhao, Zhi Wang, Yahui Han, Xinfu Zhang
Summary: Drying is an important factor in the formation of green tea flavor. By adjusting drying temperature, green tea with different aromas can be obtained. Volatile and non-volatile compounds contribute differently to the different aromas, and these compounds are related to the taste characteristics of the tea.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Fei-Long Liu, Tian-Tian Ye, Jiang-Hui Ding, Xiao-Ming Yin, Xiao-Ke Yang, Wei-Hua Huang, Bi-Feng Yuan, Yu-Qi Feng
Summary: Researchers synthesized paired reagents of DMPI and d(3)-DMPI, which can efficiently react with phosphate groups on polar phosphorylated metabolites. They found that tagging DMPI onto ribonucleotides significantly increased ionization efficiencies during LC/MS analysis, resulting in enhanced detection sensitivities.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Tobias Schlappack, Matthias Rainer, Nikolaus Weinberger, Gunther K. Bonn
Summary: This study describes the use of modified halloysite nanotubes as a novel solid material for the efficient solid phase extraction of toxic pyrrolizidine alkaloids. The optimized method showed high extraction efficiencies and overall recoveries for a mixture of pyrrolizidine alkaloids using UHPLC-MS/MS analysis. The novel solid phase material exhibited high selectivity and competitiveness compared to a commercial procedure.
ANALYTICAL METHODS
(2022)
Article
Biochemical Research Methods
Jingfu Zhao, Wenjing Peng, Xue Dong, Yehia Mechref
Summary: Protein-based therapeutics like mAbs play a crucial role in modern medicine, with glycosylation being a key quality attribute affecting the safety and efficacy of biotherapeutics. An LC-MS/MS-based glycoproteomics approach was used to characterize Fc glycans of two NISTmAb samples, revealing differences in galactosylation levels between the modified and unmodified NISTmAb. Additionally, high abundance glycan structures were identified along with relatively low levels of certain glycans and unglycosylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Analytical
Keabetswe Masike, Maria A. Stander, Andre de Villiers
Summary: Ion mobility spectrometry (IMS) plays a significant role in natural product analysis by enhancing the quality of mass spectral data, discriminating structurally similar secondary metabolites, and serving as an additional identification criterion.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Review
Chemistry, Analytical
Rui Su, Tongtong Yang, Xinrui Zhang, Na Li, Xingying Zhai, Huanwen Chen
Summary: Breath analysis is an attractive and non-invasive strategy for monitoring metabolic dynamics in living organisms. Mass spectrometry is a powerful analytical platform for breath analysis, and ambient mass spectrometry has been widely adopted due to its minimal/sample pretreatment requirements. This tutorial review introduces the principles, setup, procedures, applications, and future prospects of mass spectrometry-based techniques for breath analysis, aiming to facilitate further development and application in this field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)