Review
Agriculture, Multidisciplinary
Dae-yeol Ye, Jo Hyun Moon, Gyoo Yeol Jung
Summary: Industrial microbes like Escherichia coli are used as an alternative to petrochemical synthesis to produce various chemicals, including dicarboxylic acids (DCAs), which have potential applications in biodegradable polymers. The demand for biodegradable polymers is increasing, leading to the development of efficient production pathways using E. coli for C4 and C5 DCAs derived from central carbon metabolism. This review discusses recent advancements in metabolic engineering techniques for the production of C4 and C5 DCAs in E. coli.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Woo Sang Park, Kwang Soo Shin, Hyun Wook Jung, Yongjoo Lee, Chandran Sathesh-Prabu, Sung Kuk Lee
Summary: This study systematically evaluated the effects of metabolic engineering strategies to enhance free fatty acid production in Escherichia coli, resulting in a significant increase in FFA yield. Combinatorial metabolic engineering approaches showed potential for large-scale production of FA-derived chemicals and fuels.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Jie Cheng, Juan Li, Linggang Zheng
Summary: 1,4-Butanediol is a significant commodity chemical currently produced through energy-intensive processes with severe environmental impacts. As market demand increases, attention has shifted towards sustainable bioproduction methods, leading to advancements in metabolic engineering strategies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Xinrui Dong, Bo Liu, Yihong Bao, Weifeng Liu, Yong Tao
Summary: By co-overexpressing and optimizing the expression of key enzymes involved in violaxanthin biosynthesis, we have developed an efficient Escherichia coli strain capable of producing a large amount of violaxanthin. This is the first report of a metabolically engineered microbial platform that could be used for the commercial production of violaxanthin.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Hai-De Wang, Jian-Zhong Xu, Wei-Guo Zhang
Summary: In this study, a high L-arginine (L-Arg) producing E. coli strain was obtained through metabolic engineering. The genes speC and speF were found to play important roles in L-Arg accumulation. Feedback inhibition of the L-Arg pathway in E. coli was successfully overcome by combining gene mutations/deletions. Additionally, the insertion of a gene cluster from Corynebacterium glutamicum enhanced the metabolic flux of the L-Arg pathway. The final strain achieved the highest reported level of L-Arg production by E. coli.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: Efforts to improve the production efficiency of (R)-1,3-BDO by Escherichia coli through different metabolic engineering strategies have successfully optimized key pathway enzymes, increased NADPH supply, optimized fermentation conditions, and reduced byproducts formation. The best engineered strain can efficiently produce (R)-1,3-BDO with a yield of 0.6 mol/mol glucose, corresponding to 60% of the theoretical yield. Additionally, the feasibility of aerobically producing 1,3-BDO via a new pathway using 3-hydroxybutyrate as an intermediate has been demonstrated.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Agricultural Engineering
Shunqing Xu, Bin Zhang, Wanhe Chen, Kai Ye, Jian Shen, Pengfu Liu, Jiequn Wu, Hong Wang, Xiaohe Chu
Summary: This article describes the development of a microbial cell factory in Escherichia coli that produces ectoine at high titers, aiming to develop efficient and environmentally friendly bio-based ectoine production technology. The research provides important achievements and potential for industrial applications.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Xue Sun, Zetao Peng, Chang Li, Yanling Zheng, Ya'nan Cheng, Jianfei Zong, Fuping Lu, Yu Li, Qinggang Li
Summary: In this study, E. coli MG1655 was engineered to achieve high production of 2'-FL. The expression of 2'-FL synthesis pathway genes was finely regulated and branch pathway genes were deleted. The resulting strain produced 46.06 g/L of 2'-FL in a 5-L fermenter. Through adaptive laboratory evolution, a mutation in the rpoC gene was obtained, leading to improved cell tolerance and 2'-FL production of up to 61.06 g/L. This work provides a strategy for improving 2'-FL accumulation and has important implications for constructing high-level 2'-FL producers in the future.
BIORESOURCE TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Govinda R. Navale, Mahesh S. Dharne, Sandip S. Shinde
Summary: Isoprenoids, also known as terpenoids, are diverse organic compounds found abundantly in nature, with various applications in pharmaceutical, nutraceutical, and chemical industries. Recent advancements in metabolic engineering have enabled the production of isoprenoids in heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Challenges and strategies for scale-up and engineering of isoprenoids in heterologous host systems have been discussed in detail.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Mengyang Lei, Zetian Qiu, Leilei Guan, Zheng Xiang, Guang-Rong Zhao
Summary: In this study, a metabolic engineering approach was employed to produce Z,Z-farnesol (Z,Z-FOH) in Escherichia coli. By testing various enzymes and mutants, the optimal strain was able to achieve the highest reported titer of Z,Z-FOH in microbes. This work not only provides insights into the de novo biosynthesis of Z,Z-FOH in E. coli, but also lays a foundation for the development of synthetic E. coli cell factories for other cis-configuration terpenoids.
Article
Agriculture, Multidisciplinary
Yan Wang, Shenting Zhou, Qian Liu, Seong-Hee Jeong, Liyan Zhu, Xiangming Yu, Xiaojian Zheng, Gongyuan Wei, Seon-Won Kim, Chonglong Wang
Summary: This study optimized an alpha-santalene synthetic operon in E. coli by manipulating ribosome binding sites (RBSs). Deletion of the tnaA gene led to an increase in alpha-santalene production. These results suggest that optimizing the RBS sets of the synthetic module and attenuating competitive pathways are promising approaches for improving terpenoid production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Xue Liu, Lingling Li, Guang-Rong Zhao
Summary: Using systems metabolic engineering strategies, a three-strain commensalistic Escherichia coli coculture was designed and developed for the de novo production of genistein. This platform offers a flexible and versatile approach for the production of flavonoids and holds promise for the production of complex natural products in engineered E. coli.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Dengwei Lei, Zetian Qiu, Jihua Wu, Bin Qiao, Jianjun Qiao, Guang-Rong Zhao
Summary: This study successfully improved the de novo production of nerol and borneol in E. coli by combining metabolic and plant monoterpene synthase engineering, achieving the highest reported titers of nerol and borneol in microbes to date.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Yoojin Chong, Bong-Gyu Kim, Yeo-Jin Park, Youri Yang, Shin-Won Lee, Youngshim Lee, Joong-Hoon Ahn
Summary: In this study, a pathway was introduced to Escherichia coli to synthesize four flavonoid C-glucosides. The synthesis pathway involved the use of two regioselective flavonoid C-glycosyltransferases and resulted in the synthesis of isovitexin, vitexin, kaempferol 6-C-glucoside, and kaempferol 8-C-glucoside. The synthesized flavonoid C-glucosides provide a basis for investigating their novel biological properties.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Hao Zhang, Zhong Liang, Ming Zhao, Yanqin Ma, Zhengshan Luo, Sha Li, Hong Xu
Summary: In this study, an ectoine-producing variant of E. coli was constructed using genetic manipulation and optimized for increased production. The highest ectoine titer produced by E. coli was achieved, showing great industrial prospects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)