Article
Immunology
Alvaro Teijeira, Saray Garasa, Maria del Carmen Ochoa, Assunta Cirella, Irene Olivera, Javier Glez-Vaz, Maria Pilar Andueza, Itziar Migueliz, Maite Alvarez, Maria Esperanza Rodriguez-Ruiz, Ana Rouzaut, Pedro Berraondo, Miguel F. Sanmamed, Jose L. Perez Gracia, Ignacio Melero
Summary: IL-8 plays a key role in guiding neutrophils and monocytes/macrophages through chemotaxis; IL-8-induced NETosis is less dependent on G-proteins and can be inhibited by extracellular Ca+2 chelation; NETosis can be more readily inhibited with anti-CXCR1 antibody than chemotaxis.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Allan Prichard, Lisa Khuu, Laura C. Whitmore, Daniel Irimia, Lee-Ann H. Allen
Summary: Hypersegmentation of neutrophils induced by Helicobacter pylori infection impairs chemotaxis and inhibits dynamic changes in myosin IIA contractility and front-to-back polarity, providing new insights into PMN plasticity and H. pylori pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Mario Meza-Segura, James R. Birtley, Ana Maldonado-Contreras, Christian Mueller, Karl J. Simin, Lawrence J. Stern, Beth A. McCormick
Summary: Shigella spp. are highly adapted pathogens that cause bacillary dysentery by disrupting the intestinal epithelial barrier, with SepA releasing a neutrophil chemoattractant that promotes Shigella invasion.
Article
Immunology
Samuel Lebourgeois, Ambroise David, Houssem Redha Chenane, Vanessa Granger, Reyene Menidjel, Nadhira Fidouh, Benoit Noel, Olivier Delelis, Clemence Richetta, Charlotte Charpentier, Sylvie Chollet-Martin, Diane Descamps, Benoit Visseaux, Luc de Chaisemartin
Summary: This study discovers that SARS-CoV-2 virus can directly activate neutrophils in COVID-19, and different variants of the virus have varying effects on neutrophil activation, mirroring the differences in clinical severity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Mutsuko Kukimoto-Niino, Kazushige Katsura, Rahul Kaushik, Haruhiko Ehara, Takeshi Yokoyama, Tomomi Uchikubo-Kamo, Reiko Nakagawa, Chiemi Mishima-Tsumagari, Mayumi Yonemochi, Mariko Ikeda, Kazuharu Hanada, Kam Y. J. Zhang, Mikako Shirouzu
Summary: This study presents the cryo-electron microscopy structure of the active ELMO1-DOCK5 complex bound to Rac1, revealing that the PH domain of ELMO1 enhances the GEF activity of DOCK5 through specific interactions with Rac1. The complex alpha-helical scaffold between ELMO1 and DOCK5 stabilizes the binding of Rac1, providing insights into how ELMO modulates the biochemical activity of DOCK and achieves Rac selectivity.
Article
Biochemistry & Molecular Biology
Natalie Gray, Maren M. Limberg, Daniela Wiebe, Tobias Weihrauch, Anna Langner, Nicola Brandt, Anja U. Braeuer, Ulrike Raap
Summary: The interaction between S1P and basophils may play an important role in the pathophysiology of atopic diseases. S1P affects the function of basophils and basophils from atopic patients have reduced chemotactic activity in response to S1P.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Tilman E. Klassert, Martin Hoelzer, Cristina Zubiria-Barrera, Julia Bethge, Esther Klaile, Mario M. Mueller, Manja Marz, Hortense Slevogt
Summary: Neutrophils exhibit distinct transcriptional profiles and stress-related pathways in response to different fungal pathogens compared to bacterial pathogens, highlighting their functional diversity and adaptability. The transcriptional response is largely redundant across all pathogens in immune-relevant categories and cytokine pathway activation, but differences in the magnitude of differentially expressed genes (DEGs) are observed, with Aspergillus fumigatus inducing a lower transcriptional effect. Specific gene signatures associated with cell death are differentially regulated by fungal pathogens, potentially increasing neutrophil susceptibility to autophagy, pyroptosis, and neutrophil extracellular trap (NET) formation. These findings provide valuable insights into the complex immunological responses of neutrophils during fungal infections.
Article
Veterinary Sciences
Yanbing Li, Hechuan Wang, Shuo Wang, Qun Zhang, Han Zhang, Tianfeng Li, Qian Wang, Minghui Guo, Heze Feng, Yulun Song, Guosheng Wei, Jingchun Li
Summary: After artificial insemination, the presence of immune cells in the genital tract can cause inflammation and negatively affect sperm quality. This study investigated the effects of methylprednisolone on preserving boar sperm quality during in vitro liquid preservation and on the chemotaxis and phagocytosis of immune cells towards sperm. The results showed that a specific concentration of methylprednisolone improved sperm motility, antioxidant capacity, and inhibited immune cell activity towards sperm.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Reproductive Biology
Jing-Chun Li, Shuo Wang, He-Chuan Wang, Qun Zhang, Qian Wang, Ming-Hui Guo, Han Zhang, Tian-Feng Li, Qing Guo, Yan-Bing Li, Guo-Sheng Wei
Summary: This study investigated the effects of dexamethasone on the chemotaxis and phagocytic activity of neutrophils for spermatozoa and the semen quality of preserved boar semen. The results showed that dexamethasone inhibited the activity of neutrophils and had the best preservation effect on boar semen at a concentration of 1 x 10-6 mol/mL under storage conditions of 17 degrees C.
Article
Immunology
Verena Vonwirth, Yagmur Buelbuel, Anke Werner, Hakim Echchannaoui, Johannes Windschmitt, Alice Habermeier, Sonia Ioannidis, Niu Shin, Roland Conradi, Matthias Bros, Stefan Tenzer, Matthias Theobald, Ellen Ildicho Closs, Markus Munder
Summary: The depletion of arginine by myeloid cell arginase is a key mechanism of tumor immune escape by inhibiting T cell functions. Inhibiting arginase 1 in polymorphonuclear neutrophil granulocytes leads to hyperactivation of T cells, enhancing their proliferation and cytokine production, which could be important for preventing human tumor immune escape.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Xiaomin Ma, Xiaoxiao Ma, Lihui Zhu, Yunxue Zhao, Mengmeng Chen, Tao Li, Yueke Lin, Dapeng Ma, Caiyu Sun, Lihui Han
Summary: In this study, we found that MG53 acts as an inhibitor of RAC1 and a tumor suppressor in hepatocellular carcinoma (HCC) by catalyzing RAC1 ubiquitination and inhibiting RAC1 activity. Furthermore, MG53 suppressed the malignant behaviors of HCC cells and enhanced chemosensitivity to sorafenib treatment.
Article
Biochemistry & Molecular Biology
Sunho An, Tam Thuy Lu Vo, Taekwon Son, Hoon Choi, Jinyoung Kim, Juyeon Lee, Byung Hoon Kim, Misun Choe, Eunyoung Ha, Young-Joon Surh, Kyu-Won Kim, Ji Hae Seo
Summary: This study reveals that SAMHD1 is an oncogene that plays a pivotal role in promoting cell migration in renal clear cell carcinoma. SAMHD1 enhances cell motility by promoting lamellipodia formation and the formation of endosomal complexes.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Immunology
Xin Yu, Lu Li, Menghao Zhang, Jinjing Liu, Hua Chen, Fengchun Zhang, Wenjie Zheng
Summary: This study identified specific gene signatures in neutrophils from Behcet's disease (BD) patients and revealed the functional pathways associated with these genes. The findings suggest that BD neutrophils display enhanced chemotaxis, which is associated with the overexpression of CCL5, CCR6, and ETS1.
CLINICAL IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Markus Waldeck-Weiermair, Shambhu Yadav, Jonas Kaynert, Venkata Revanth Thulabandu, Arvind K. Pandey, Fotios Spyropoulos, Taylor Covington, Apabrita Ayan Das, Christina Kruger, Thomas Michel
Summary: Statins have multiple protective effects on the cardiovascular system, including lowering cholesterol levels and antioxidant effects. Simvastatin, a statin drug, inhibits the production of oxidant H2O2 induced by histamine and vascular endothelial growth factor, and also regulates the localization of signaling protein Rac1.
Article
Cell Biology
Fabian Bock, Bertha C. Elias, Xinyu Dong, Diptiben Parekh, Glenda Mernaugh, Olga M. Viquez, Anjana Hassan, Venkateswara Rao Amara, Jiageng Liu, Kyle L. Brown, Andrew S. Terker, Manuel Chiusa, Leslie S. Gewin, Agnes B. Fogo, Cord H. Brakebusch, Ambra Pozzi, Roy Zent
Summary: The study demonstrates that Rac1 is not essential for epithelial branching morphogenesis during kidney collecting duct development, but plays a crucial role in maintaining epithelial integrity, polarity, and function by Arp2/3-dependent cytoskeletal branching. This function of Rac1 involves restricting actomyosin activity. Despite a mild developmental phenotype in mice with selectively deleting Rac1 at the initiation of UB development, aging leads to disruption of epithelial integrity and function in the collecting duct, which is reversible with direct myosin II inhibition. Additionally, Rac1 controls lateral membrane height and overall epithelial morphology by restricting actomyosin and promoting CD epithelial integrity.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Noah Fine, Ioannis D. Dimitriou, Jacob Rullo, Maria Jose Sandi, Bjorn Petri, Jack Haitsma, Hisham Ibrahim, Jose La Rose, Michael Glogauer, Paul Kubes, Myron Cybulsky, Robert Rottapel
JOURNAL OF CELL BIOLOGY
(2016)
Article
Immunology
Linda Vong, Robert J. Lorentz, Amit Assa, Michael Glogauer, Philip M. Sherman
JOURNAL OF IMMUNOLOGY
(2014)
Article
Dentistry, Oral Surgery & Medicine
M. Landzberg, H. Doering, G. M. Aboodi, H. C. Tenenbaum, M. Glogauer
JOURNAL OF PERIODONTAL RESEARCH
(2015)
Article
Cell Biology
Madonna R. Peter, Mirjana Jerkic, Valentin Sotov, David N. Douda, Daniela S. Ardelean, Niousha Ghamami, Flavia Lakschevitz, Meraj A. Khan, Susan J. Robertson, Michael Glogauer, Dana J. Philpott, Nades Palaniyar, Michelle Letarte
MEDIATORS OF INFLAMMATION
(2014)
Article
Cell Biology
Ronald S. Flannagan, Johnathan Canton, Wendy Furuya, Michael Glogauer, Sergio Grinstein
MOLECULAR BIOLOGY OF THE CELL
(2014)
Review
Dentistry, Oral Surgery & Medicine
William Khoury, Judah Glogauer, Howard C. Tenenbaum, Michael Glogauer
JOURNAL OF PERIODONTAL RESEARCH
(2020)
Article
Dentistry, Oral Surgery & Medicine
N. Fine, J. W. Chadwick, C. Sun, K. K. Parbhakar, N. Khoury, A. Barbour, M. Goldberg, H. C. Tenenbaum, M. Glogauer
Summary: The presence of periodontal diseases is closely related to other chronic inflammatory conditions such as cardiovascular disease, diabetes, and arthritis. Studies show that periodontal tissue inflammation affects the production and activation of polymorphonuclear neutrophils (PMNs), contributing to a hyperinflammatory response in the body. This systemic effect of PD on immune response can exacerbate inflammatory reactions when faced with secondary infections, highlighting the complex interactions between PD and other inflammatory diseases.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Immunology
Noah Fine, Eric Gracey, Ioannis Dimitriou, Jose La Rose, Michael Glogauer, Robert Rottapel
JOURNAL OF IMMUNOLOGY
(2020)
Review
Dentistry, Oral Surgery & Medicine
Abdelahhad Barbour, Judah Glogauer, Lis Grinfeld, Rouzbeh Ostadsharif Memar, Noah Fine, Howard Tenenbaum, Michael Glogauer
Summary: The significant progress in molecular biology has led to the revolutionary application of CRISPR-Cas systems in clinical research and medicine. This biological tool has been utilized for gene manipulation and novel therapeutic development, particularly showing potential in personalized periodontal care, although challenges still exist in its clinical application.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Afisu Oladega, Dorna Mojdami, Andrew Hope, Erin Watson, Michael Glogauer
Summary: This study aimed to develop consensus guidelines for dental care in head and neck cancer patients undergoing RT using the Modified Delphi Technique. Through three rounds of surveys and a virtual meeting, consensus was achieved in most questions across all domains, providing a basis for guideline development.
JOURNAL OF EVIDENCE-BASED DENTAL PRACTICE
(2021)
Article
Cell Biology
Rana Badewy, Amir Azarpazhooh, Howard Tenenbaum, Kristin L. L. Connor, Jim Yuan Lai, Michael Sgro, Richard P. P. Bazinet, Noah Fine, Erin Watson, Chunxiang Sun, Sourav Saha, Michael Glogauer
Summary: This prospective study found that maternal oral inflammation may affect human milk composition, including neutrophil counts, activation state, and fatty acid levels. Increasing severity of oral inflammation was associated with changes in specific markers expression and fatty acid levels in human milk, suggesting a link between oral health and breastfeeding outcomes.
Article
Dentistry, Oral Surgery & Medicine
Rana Badewy, Elaine Cardoso, Michael Glogauer, Michael Sgro, Kristin L. Connor, Jim Yuan Lai, Richard P. Bazinet, Howard C. Tenenbaum, Amir Azarpazhooh
Summary: This study investigates the association between maternal oral inflammatory load (OIL), sociodemographic characteristics, and the oral health-related quality of life (OHRQoL) in postpartum women. The results showed that sociodemographic characteristics significantly impact the OHRQoL of mothers, highlighting the importance of considering these factors when implementing targeted preventive dental care programs for mothers.
JOURNAL OF PERIODONTOLOGY
(2023)
Review
Dentistry, Oral Surgery & Medicine
Yuval Peled, Cameron A. Stewart, Michael Glogauer, Yoav Finer
Summary: Based on recent literature evidence, this review highlights the involvement of bacteria and neutrophils, among other factors, in the degradation of tooth components and dental restoratives, leading to caries and restoration failure.
Review
Microbiology
Abdelahhad Barbour, Omnia Elebyary, Noah Fine, Morvarid Oveisi, Michael Glogauer
Summary: More than 700 bacterial species live in the oral cavity, producing a wide range of bioactive metabolites crucial for oral health maintenance. Next-generation sequencing has revolutionized our understanding of the oral microbiome and its significance in health and disease. However, research on unique oral microbial metabolites is still limited. This article discusses the importance of microbial metabolites in oral health and disease, as well as the potential of genomics and genome mining in discovering novel bioactive metabolites from the human oral microbiome.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Cell Biology
Jeffrey W. Chadwick, Noah Fine, William Khoury, Nikola Tasevski, Chun-Xiang Sun, Parastoo Boroumand, Amira Klip, Michael Glogauer
Summary: The study revealed consistent detectable neutrophil populations in various tissues of healthy mice, with tissue-specific immunophenotypes; neutrophils from biofilm-associated mucosal tissues showed higher expression of surface markers of activation and altered immunophenotypes in the presence of an inflammatory stimulus.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)