Article
Agriculture, Multidisciplinary
Jiandong Shen, Qixing Jiang, Wei Zhang, Yanshun Xu, Wenshui Xia
Summary: In this study, four active bands of gelatinolytic proteinases were identified in grass carp fillets, with metalloproteinase and serine proteinase playing critical roles in collagen breakdown during the initial days of chilling, and cysteine proteinase becoming more prominent later in the process.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Fisheries
Feifei Chen, Ziyi Zhang, Li Wang, Haodong Yu, Xuezhen Zhang, Keming Rong
Summary: This study investigated the optimal dietary protein supplementation level for grass carp. The results showed that the highest body weight and growth rate were achieved when the protein content was 2.27%, and antioxidant enzyme activities were also significantly increased.
Article
Fisheries
Chuxin Wu, Shina Lu, Jihuan Hu, Xunhang Li, Dong Yao, Meifang Zhang
Summary: The study identified a teleost SNX8 cDNA from grass carp, which was significantly up-regulated after infection and mainly distributed in the endoplasmic reticulum. CiSNX8 may negatively regulate the RLR signaling pathway and interact with multiple proteins, suggesting its involvement in innate immune response through different molecular mechanisms.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Food Science & Technology
Yi Liu, Shu Kang, Haijuan Zhang, Yi Kai, Hongshun Yang
Summary: This study investigated the effects of fish gelatin/chitosan-based films incorporated with lemon essential oil on grass carp fillets during chilled and iced storage. The results showed that the active films inhibited moisture transformation and improved the water-holding capacity of the fish samples. Additionally, the films effectively reduced the growth of bacteria and preserved the freshness of the samples. The combination of the active films and iced storage provided a promising strategy to prolong the shelf-life of grass carp fillets.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Immunology
Wenjie Guo, Rui Han, Weizhen Xu, Zijun Lu, Yanwei Li, Xueming Dan, Zequan Mo
Summary: This study investigated the immune protective effects of an inactivated F. columnare vaccine. The results showed that when white oil adjuvant was used as a booster, the relative percent survival (RPS) after 8 weeks of vaccination was 34% and 61%, respectively. Furthermore, the inactivated vaccine comprising an appropriate antigen isolate with white oil adjuvant as a booster can provide effective protection in grass carp.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Jian Sun, Wenyi Wu, Xinxin Xu, Hong Ji
Summary: The study illustrates that excessive lipid accumulation in farmed fish liver may be a protective response to exogenous fatty acids. Inhibition of DGAT1 and DGAT2 in grass carp hepatocytes shows that DGAT1 is responsible for lipid droplet formation while DGAT2 promotes lipid droplet fusion. This indicates a differential role of DGAT1 and DGAT2 in lipid metabolism and liver health in farmed fish.
Article
Food Science & Technology
Jiandong Shen, Dawei Yu, Pei Gao, Yanshun Xu, Qixing Jiang, Wenshui Xia
Summary: The aim of this study was to investigate the mechanisms of softening texture in chilled grass carp fillets. The results showed that collagen solubility increased and gelatinolytic proteinase activity was activated, correlating with texture softening.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Fisheries
Yanan Li, Zhijie Lu, Fanbin Zhan, Minxuan Yang, Lijuan Zhao, Fei Shi, Jun Li, Li Lin, Zhendong Qin
Summary: The study revealed that the Nrf2 gene in grass carp can regulate antioxidant balance and participate in the immune response against bacterial invasion, which is crucial for disease prevention and control in the fish farming industry.
Article
Environmental Sciences
Xiao Liang, Yin Wang, Lu Liu, Xi Zhang, Li Li, Rong Tang, Dapeng Li
Summary: This study investigated the effects of nitrite exposure on thyroid hormone levels and intestinal iodothyronine deiodinases activity in grass carp. The results showed that nitrite exposure altered thyroid hormone levels, morphology of thyroid follicles, and gene expressions related to thyroid hormone metabolism.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Yang Xu, Yuting Gui, Dan Zhi, Jie Pi, Xinhua Liu, Jianguo Xiang, Deliang Li, Junhua Li
Summary: Cadmium is a dominant metal pollutant in aquatic environments that affects ion balance, oxidative stress, and immune responses in aquatic organisms. The addition of calcium can mitigate the toxicity of cadmium by reducing its accumulation in tissues, maintaining ion balance, alleviating oxidative stress, and regulating ATPase activity and gene expression.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Fisheries
Shina Lu, Xiaojue Peng, Shanshan Zeng, Hang Deng, Zhiqing Feng, Qing Zeng, Xining Cheng, Jihuan Hu, Zuocheng Ye, Meifeng Li, Xiaowen Xu, Gang Lin, Chengyu Hu
Summary: Two mammalian PIAS1 orthologs, CiPIAS1a and CiPIAS1b, were identified in Ctenopharyngodon idellus. They are suggested to participate in IFN-mediated antiviral and antibacterial immune response by interacting with STAT1 to inhibit its phosphorylation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Microbiology
Fanshu Xiao, Lanjie Liao, Qiaoqing Xu, Zhili He, Tiaoyi Xiao, Jianjun Wang, Jie Huang, Yuhe Yu, Bo Wu, Qingyun Yan
Summary: This study observed a dysbiosis of gut microbiota in grass carp infected with the GCRV virus, with an overabundance of Cetobacterium somerae triggering exacerbated inflammatory responses through NLRs, TLRs, and LPS pathways.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Fisheries
Fei Shi, Zhijie Lu, Minxuan Yang, Feng Li, Fanbin Zhan, Lijuan Zhao, Yanan Li, Qingqing Li, Jiangtao Li, Jun Li, Li Lin, Zhendong Qin
Summary: The study showed that APS can modulate the intestinal microbiota and activate the immune response to protect grass carp from bacterial infection.
Article
Environmental Sciences
Xiao Chen, Jinfeng Yang, Zhenmin Ling, Tuoyu Zhou, Bojian Zhou, Haoyang Wang, Xiangkai Li, Pu Liu
Summary: The study found that introducing a recombinant Escherichia coli strain expressing Pb2+-specific surface-binding protein into grass carp gut can reduce Pb2+ concentration, increase the residual level of Pb2+ in feces, and alleviate oxidative stress, histological alterations, and gut microbiota dysbiosis induced by Pb2+ exposure.
ENVIRONMENTAL POLLUTION
(2021)
Article
Fisheries
Zijun Lu, Ren Gao, Yafei Duan, Rui Han, Wenjie Guo, Xueming Dan, Yanwei Li
Summary: This study isolated 45 bacterial strains of F. columnare from grass carp in various provinces of China, identifying three genotypes with genomovar I being the dominant type. Analysis revealed genetic differences among the three genomovars, with a closer relationship between genomovar I and II. The study provides insight into the genetic diversity of F. columnare in Chinese grass carp.
Article
Chemistry, Applied
You Tian, Da-Wen Sun, Zhiwei Zhu
Summary: The study investigated the properties of nature-inspired natural deep eutectic solvents (NADESs) as anti-freezing agents, finding them to possess excellent anti-freezing capacity, with Pro:Sor (1:1) showing the greatest temperature sensitivity and best anti-frosting capacity. Applications in frozen chicken breast further demonstrated the potentials of NADESs as antifreezing agents for the industry.
Article
Chemistry, Applied
You Tian, Da-Wen Sun, Liang Xu, Tian-Hao Fan, Zhiwei Zhu
Summary: By preparing bio-inspired deep eutectic solvents and gels, the anti-frosting capacity and freezing tolerance were investigated. The results showed that the gels had good hydrophilicity and high anti-frosting capacity, as well as flexibility and stable mechanical strength under extreme temperatures. The absorption-inhibition hypothesis was verified, demonstrating the great potential of the gels in interfacial anti-frosting.
FOOD HYDROCOLLOIDS
(2022)
Review
Food Science & Technology
Leyin Zeng, Zhiwei Zhu, Da-Wen Sun
Summary: This article introduces the application of graphene oxide (GO) membranes in the food industry and discusses the key factors affecting their permeability. It also highlights the potential applications and future development directions of GO membranes in food material filtration and packaging.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Chemistry, Multidisciplinary
You Tian, Da-Wen Sun, Liang Xu, Tian-Hao Fan, Shu-Ti Zhang, Zhiwei Zhu
Summary: This study investigated the use of natural deep eutectic solvents (NADESs) as cryoprotectants for cytotoxicity-free and green cryopreservation. The results showed that NADESs could be safe and biocompatible, with excellent cryoprotective efficiency. Furthermore, NADES-cryopreserved cells exhibited better morphology and lower apoptosis compared to cells cryopreserved with glycerol and DMSO.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Applied
Clement Kehinde Ajani, Zhiwei Zhu, Da-Wen Sun
Summary: Dynamic investigation on the effects of vacuum cooling on cellular water transport and structural changes of steamed bread was conducted using NMR/MRI technique. The study revealed that vacuum cooling caused water loss and structural changes in steamed bread, and the initial condition of the food also influenced the vacuum cooling process.
Article
Chemistry, Applied
Dongmei Li, Zhiwei Zhu, Da-Wen Sun
Summary: This study developed an improved method to directly visualize the spatial distribution of water content and hydrogen bonding state in apple and potato cells, comparing the differences in water distribution. The results demonstrated that the method could successfully quantify the content and hydrogen bonding state of water in fruit and vegetable cells.
Article
Chemistry, Applied
Swati Mahato, Da-Wen Sun, Zhiwei Zhu
Summary: This study investigated the effects of electromagnetic fields with frequencies between 100 and 400 Hz on cold storage of grass carp. The results showed that EMF treatment at frequencies between 100 and 400 Hz could improve the quality of grass carp during cold storage by increasing Ca(2+)ATPase activities and reducing protein oxidation. The effects on lipid oxidation varied with different frequencies.
Article
Engineering, Chemical
Clement Kehinde Ajani, Zhiwei Zhu, Da-Wen Sun
Summary: In this study, a conjugate modelling approach was used to investigate the momentum, heat, mass transport, and structural deformation during vacuum cooling of steamed bread. The results showed that the three-parameter Maxwell model accurately described the viscoelastic behavior of the bread, and the model predictions were accurate for moisture variation and areal shrinkage. Sensitivity analysis revealed that the deformation model was more sensitive to water transport parameters, particularly the hydrous compressibility factor.
JOURNAL OF FOOD ENGINEERING
(2023)
Article
Food Science & Technology
Dongmei Li, Zhiwei Zhu, Da-Wen Sun
Summary: The effects of high-pressure freezing and deep-frozen storage at -80 degrees C on Cordyceps sinensis (CS) samples were investigated and compared with other freezing methods. The study found that liquid nitrogen freezing combined with deep-frozen storage caused minimal damage to cell integrity and viability, while high-pressure shift freezing combined with deep-frozen storage resulted in the slowest deterioration of these attributes. The findings suggest that liquid nitrogen freezing combined with deep-frozen storage is the best method for freezing CS samples, while high-pressure shift freezing combined with deep-frozen storage may be a better option for long-term preservation of highly perishable fresh products with a cellular structure.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Environmental
Liang Xu, Da-Wen Sun, You Tian, Tianhao Fan, Zhiwei Zhu
Summary: A polyacrylamide/polyvinyl alcohol hydrogel with nanoparticles (NPs/NADES@PAAm/PVA) was fabricated for highly efficient daytime passive cooling. This hydrogel exhibited a high solar reflectance and atmospheric emissivity, as well as robust mechanical strength, excellent flexibility, and great rehydration. It could achieve simultaneous radiative and evaporative cooling, and demonstrated promising application potential in building cooling and food preservation.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Food Science & Technology
Swati Mahato, Zhiwei Zhu, Da-Wen Sun
Summary: This study examined the potential significance of extremely low-frequency electromagnetic fields (ELF-EMF) at frequencies ranging from 40 to 70 Hz in inducing biological effects during chilled storage of grass carp. The results showed that treatment with 50 and 60 Hz frequencies significantly increased Ca2+ATPase activity and pH, while reducing the total sulfhydryl content. The treatment at 50 Hz had the most pronounced effects, indicating a potential "window" effect in enhancing the quality of myofibrillar protein during chilled storage.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Swati Mahato, Zhiwei Zhu, Da-Wen Sun
Summary: This study aimed to investigate how different frequencies of electromagnetic field (EMF) affect the freezing of grass carp tissue. Results showed that treatment with frequencies between 200 and 400 Hz significantly increased the initial freezing point, while the phase change duration remained unchanged. The application of EMF also led to reductions in ice areas and crystal sizes. Overall, these findings suggest that frequencies between 200 and 400 Hz are effective in improving the freezing process with the assistance of electromagnetic field.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Zhiwei Zhu, Hui Liang, Da-Wen Sun
Summary: In this study, two superhydrophobic surfaces (SHS) were created by spraying hexadecyltrimethoxysilane (HDTMS) and stearic acid (SA)-modified SiO2 nanoparticles (NPs) suspensions onto aluminum (Al) substrates coated with epoxy resin, and then infusing food-safe silicone and camellia seed oils into the SHS, respectively, achieving anti-frosting/icing performance. The SLIPS exhibited excellent frost resistance and defrost properties, with ice adhesion strength much lower than that of SHS. The SLIPS showed great potential for developing into robust anti-icing/frosting materials for the freezing industry.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Chemical
Clement Kehinde Ajani, Zhiwei Zhu, Da-Wen Sun
Summary: A microscale modelling framework was developed to compute effective properties related to flow, heat, and mass transfer during vacuum cooling of porous foods. The model was validated using nuclear magnetic resonance to investigate cellular water transport in steamed bread. The computed effective properties were in line with experimental measurements, and the analysis showed that microstructural variability significantly affected the estimated effective properties. The microscale model provided details not visible in the lumped formulation, offering a better understanding of moisture loss mechanisms during vacuum cooling.
TRANSPORT IN POROUS MEDIA
(2023)
Article
Food Science & Technology
Libin Sun, Zhiwei Zhu, Da-Wen Sun
Summary: Freezing reduces the temperature of the food system and extends the shelf life of food. However, the formation of ice crystals could impact food quality. Ice-regulating materials have been proven to regulate ice nucleation, growth, and recrystallization, showing promising applications in the food industry.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)