Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Microbiology
Issa Sy, Lena Margardt, Emmanuel O. Ngbede, Mohammed I. Adah, Saheed T. Yusuf, Jennifer Keiser, Jacqueline Rehner, Jurg Utzinger, Sven Poppert, Soren L. Becker
Summary: Fascioliasis is caused by Fasciola gigantica and Fasciola hepatica. Traditional diagnostic methods have low accuracy, while molecular diagnostics such as MALDI-TOF MS are more reliable. A study developed and validated an in-house database for species-specific identification of Fasciola using MALDI-TOF MS, showing promising results for accurate identification of medically relevant liver flukes.
Article
Immunology
John A. Fissel, Carrie K. Holdren-Serrell, Warda Memon, Sean X. Zhang
Summary: One of the challenges in fungal infection treatment is the long time required for species identification. MALDI-TOF MS has shown potential in rapidly identifying bacteria and yeasts, and this study explores its use for identifying filamentous molds. Most isolates in the study could be identified by MALDI-TOF MS within three days.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Hui Li, Chunxia Ma, Meng Gao, Yong Li, Lei Xie, Dantong Zhao, Runhao Zhang, Guanhua Zhang, Wenhan Li, Rong Rong, Biao Kong
Summary: Two-dimensional material Ti3C2(OH)(x) shows potential for overcoming the limitations of MALDI-TOF-MS in analyzing small-molecular compounds. It offers advantages such as minimum background interference, high peak intensity, high salt and protein tolerance, good repeatability, and stability after storage.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Review
Microbiology
Xin-Fei Chen, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, Po-Ren Hsueh
Summary: MALDI-TOF MS has been used in clinical microbiology since 2010, offering advantages such as convenience, speed, accuracy, and low cost. It has shown promising results in rapid detection of highly virulent and drug-resistant pathogens, making it a valuable tool in clinical microbial identification.
Article
Infectious Diseases
Jichan Shi, Gexin Gao, Jing Pan, Lian-Peng Wu, Hongye Ning, Zhengxin Wu, Xinchun Ye, Xiangao Jiang
Summary: This study evaluated the clinical value of MALDI-TOF MS in detecting NTM by comparing the results with liquid culture and gene chip. The findings showed that MALDI-TOF MS had a high consistency with both methods and could be a rapid and effective method for identifying NTM strains and drug resistance.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Article
Chemistry, Analytical
Yuanxia Lv, Xiaohuan Qin, Kun Hu, Yong Huang, Shulin Zhao
Summary: The novel matrix-assisted laser desorption/ionization time-of-flight mass spectrometric method developed based on MoS2/g-C3N4 shows enhanced UV absorption and improved desorption and ionization efficiency for highly sensitive small molecular compounds detection. It also exhibits low detection limits, good reproducibility, and capability to directly determine uric acid in complex biological samples.
Article
Plant Sciences
Ye Rin Jin, Myung Jin Oh, Heung Joo Yuk, Hyun Joo An, Dong Seon Kim
Summary: This study established a new mass spectrometry-based analysis procedure for red ginseng polysaccharides (RGPs), successfully qualitatively analyzing neutral and acidic RGPs, and identifying novel components. The new procedure shows great potential as a specific tool for searching and determining various polysaccharides in herbal medicines.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Parasitology
Jurena Christiane Ebersbach, Marcello Otake Sato, Matheus Pereira de Araujo, Megumi Sato, Soeren L. Becker, Issa Sy
Summary: Schistosomiasis is a major tropical disease affecting millions of people worldwide. The current diagnostic methods have limitations, and this study developed and validated a species-specific database for adult Schistosoma identification. The study also evaluated the effects of different storage solutions on spectra profiles. The results showed that MALDI-TOF MS can reliably distinguish between S. mansoni and S. japonicum.
PARASITES & VECTORS
(2023)
Review
Food Science & Technology
Catherine M. Burgess, Andrea Gianotti, Nadia Gruzdev, John Holah, Susanne Knochel, Angelika Lehner, Edyta Margas, Stephan Schmitz Esser, Shlomo Sela (Saldinger), Odile Tresse
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2016)
Article
Food Science & Technology
Denise Althaus, Marco Jermini, Petra Giannini, Gladys Martinetti, Danuta Reinholz, Magdalena Nuesch-Inderbinen, Angelika Lehner, Roger Stephan
FOODBORNE PATHOGENS AND DISEASE
(2017)
Article
Microbiology
Hannah R. Chase, Gopal R. Gopinath, Athmanya K. Eshwar, Andrea Stoller, Claudia Fricker-Feer, Jayanthi Gangiredla, Isha R. Patel, Hediye N. Cinar, HyeJin Jeong, ChaeYoon Lee, Flavia Negrete, Samantha Finkelstein, Roger Stephan, Ben D. Tall, Angelika Lehner
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Cell Biology
Athmanya Konegadde Eshwar, Nina Wolfrum, Roger Stephan, Seamus Fanning, Angelika Lehner
CELLULAR MICROBIOLOGY
(2018)
Article
Gastroenterology & Hepatology
Gopal R. Gopinath, Hannah R. Chase, Jayanthi Gangiredla, Athmanya Eshwar, Hyein Jang, Isha Patel, Flavia Negrete, Samantha Finkelstein, Eunbi Park, TaeJung Chung, YeonJoo Yoo, JungHa Woo, YouYoung Lee, Jihyeon Park, Hyerim Choi, Seungeun Jeong, Soyoung Jun, Mijeong Kim, Chaeyoon Lee, HyeJin Jeong, Seamus Fanning, Roger Stephan, Carol Iversen, Felix Reich, Gunter Klein, Angelika Lehner, Ben D. Tall
Article
Biotechnology & Applied Microbiology
Shabarinath Srikumar, Yu Cao, Qiongqiong Yan, Koenraad Van Hoorde, Scott Nguyen, Shane Cooney, Gopal R. Gopinath, Ben D. Tall, Sathesh K. Sivasankaran, Angelika Lehner, Roger Stephan, Seamus Fanning
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Genetics & Heredity
Hyein Jang, Jungha Woo, Youyoung Lee, Flavia Negrete, Samantha Finkelstein, Hannah R. Chase, Nicole Addy, Laura Ewing, Junia Jean Gilles Beaubrun, Isha Patel, Jayanthi Gangiredla, Athmanya Eshwar, Ziad W. Jaradat, Kunho Seo, Srikumar Shabarinath, Seamus Fanning, Roger Stephan, Angelika Lehner, Ben D. Tall, Gopal R. Gopinath
STANDARDS IN GENOMIC SCIENCES
(2018)
Review
Food Science & Technology
Flavia Negrete, Hyein Jang, Jayanthi Gangiredla, JungHa Woo, YouYoung Lee, Isha R. Patel, Hannah R. Chase, Samantha Finkelstein, Caroline Z. Wang, Shabarinath Srikumar, Scott Nguyen, Athmanya Eshwar, Roger Stephan, Angelika Lehner, Seamus Fanning, Ben D. Tall, Gopal R. Gopinath
CURRENT OPINION IN FOOD SCIENCE
(2019)
Article
Genetics & Heredity
Scott Van Nguyen, Dechamma Mundanda Muthappa, Athmanya K. Eshwar, James F. Buckley, Brenda P. Murphy, Roger Stephan, Angelika Lehner, Seamus Fanning
MICROBIAL GENOMICS
(2020)
Article
Microbiology
Joao Anes, Katherine Dever, Athmanya Eshwar, Scott Nguyen, Yu Cao, Sathesh K. Sivasankaran, Sandra Sakalauskaite, Angelika Lehner, Stephanie Devineau, Rimantas Daugelavicius, Roger Stephan, Seamus Fanning, Shabarinath Srikumar
Summary: This study induced oxidative stress in K. pneumoniae MGH78578 using paraquat and identified the soxS gene as significantly induced during oxidative stress, potentially serving as a genetic target against multidrug-resistant K. pneumoniae infections. The soxS mutant exhibited reduced susceptibility to tetracycline, with impaired efflux activity leading to tetracycline accumulation in the cytoplasm. The study suggests that targeting the soxS gene could be a promising strategy for combating antimicrobial resistance in K. pneumoniae.
Article
Microbiology
Hyein Jang, Athmanya Eshwar, Angelika Lehner, Jayanthi Gangiredla, Isha R. Patel, Junia Jean-Gilles Beaubrun, Hannah R. Chase, Flavia Negrete, Samantha Finkelstein, Leah M. Weinstein, Katie Ko, Nicole Addy, Laura Ewing, Jungha Woo, Youyoung Lee, Kunho Seo, Ziad Jaradat, Shabarinath Srikumar, Seamus Fanning, Roger Stephan, Ben D. Tall, Gopal R. Gopinath
Summary: Comparative molecular and genomic analysis, as well as zebrafish infection studies, were conducted on plant-origin Cronobacter sakazakii strains. It was found that these strains possess genomic features and virulence comparable to clinical strains, indicating that these foods could potentially serve as transmission vehicles for this important foodborne pathogen.
Article
Microbiology
Angelika Lehner, Ben Davis Tall, Seamus Fanning, Shabarinath Srikumar
CURRENT CLINICAL MICROBIOLOGY REPORTS
(2018)
Article
Microbiology
Hannah R. Chase, Leo Eberl, Roger Stephan, HyeJin Jeong, Chaeyoon Lee, Samantha Finkelstein, Flavia Negrete, Jayanthi Gangiredla, Isha Patel, Ben D. Tall, Gopal R. Gopinath, Angelika Lehner
GENOME ANNOUNCEMENTS
(2017)
Article
Marc J. A. Stevens, Katrin Zurfluh, Denise Althaus, Sabrina Corti, Angelika Lehner, Roger Stephan
GENOME ANNOUNCEMENTS
(2018)
Article
Hyein Jang, Nicole Addy, Laura Ewing, Junia Jean-Gilles Beaubrun, YouYoung Lee, JungHa Woo, Flavia Negrete, Samantha Finkelstein, Ben D. Tall, Angelika Lehner, Athmanya Eshwar, Gopal R. Gopinath
GENOME ANNOUNCEMENTS
(2018)