Article
Nanoscience & Nanotechnology
Abhay Kotnala, Hongru Ding, Yuebing Zheng
Summary: Plasmonic nanoapertures have various exciting applications, but traditional nanoapertures may result in weak light-matter interactions. A new hybrid nanoaperture design, AuNIs-e-NA, provides multiple electromagnetic hotspots and can achieve up to 4000-fold field enhancements, significantly improving fluorescence signals. With simple design, easy fabrication, and strong signal enhancements, AuNIs-e-NA is a robust platform for surface-enhanced optical sensing, imaging, and spectroscopy.
Article
Chemistry, Physical
N. P. Kovalets, E. P. Kozhina, I. V. Razumovskaya, S. A. Bedin, A. A. Piryazev, Yu. V. Grigoriev, A. V. Naumov
Summary: This study investigates the hyperenhancement effect of Raman scattering (RS) appearing on microcracks of metal deposition (silver and gold) on uniaxially stretched polymer track-etched membranes. The deformation of membranes with high surface density and small pore diameter leads to the development of many microcracks in the metal coating. The efficiency of surface enhancement RS (SERS) is investigated using malachite green as an example, and the ability to recognize extremely low concentrations of the substance is demonstrated. The study finds that the SERS signal increases with the deformation of the samples and shows even greater enhancement after unloading and relaxation. Tensile strain experiments and changes in electrical conductivity confirm the hypothesis that SERS appears on microcrack edges with a relatively small distance between their boundaries. SERS technique is proposed for analyzing the formation of micro- and nanocracks on metal coatings.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Chemistry, Analytical
Tian Zhang, Haohan Song, Liyun Deng, Chaoqing Dong, Jicun Ren
Summary: Single-particle catalysis analysis is crucial for understanding the catalytic mechanism of nanocatalysts. A novel method based on photon burst counting analysis combined with a microfluidic chip has been proposed in this study to evaluate the catalytic activity of a free nanocatalyst. The experimental conditions were optimized and the method was successfully used to study the catalytic activity of gold nanoparticles with different sizes and morphologies.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Viktoria Wedler, Dustin Quinones, Heiko Peisert, Erik Schaeffer
Summary: This study presents an environmentally friendly method for functionalized surface preparation in single-molecule assays, using plasma activation with hydrocarbons for silanization. The research found that even weakly hydrophobic surfaces were optimal, and the method significantly reduced the preparation effort while maintaining high-quality surfaces.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Spectroscopy
Xiangrong Li, Wei Guo, Ruonan Xu, Zhizhi Song, Tianjun Ni
Summary: This study investigated the interaction between gold nanoparticles and various proteins using spectroscopic techniques. The results showed that gold nanoparticles can form a stable protein corona by interacting with proteins, and the nature of the interaction depends on the characteristics of the proteins.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Chemistry, Physical
Lars Jeuken, Michel Orrit, Gerard Canters
Summary: Optical studies of redox reactions are non-invasive and can be done in solution or on surfaces, however, complications arise in electrochemistry due to surface effects. These effects impact redox thermodynamics and kinetics, as well as the fluorescent properties of dyes. This article summarizes single-molecule work done on protein molecules and discusses future perspectives.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Chemistry, Physical
Subhasis Adhikari, Michel Orrit
Summary: This article reviews the progress of single-molecule optical experiments in the past 20 years and proposes some perspectives for the future. The article particularly focuses on methodological advances in fluorescence, super-resolution, photothermal contrast, and interferometric scattering and briefly discusses a few applications. These advances have allowed for the exploration of new emitters and quantum optics, the study of chemistry and biology in complex heterogeneous systems, nanoparticles, and plasmonics, and the detection and investigation of non-fluorescing and non-absorbing nano-objects. The article concludes by proposing ideas for future experiments, aiming for more and better signals and a deeper understanding of the nanoscale world of single (bio-)molecules, nanoparticles, and their nano-environments.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Stefan Krause, Evelyn Ploetz, Johann Bohlen, Patrick Schuler, Renukka Yaadav, Florian Selbach, Florian Steiner, Izabela Kaminska, Philip Tinnefeld
Summary: Researchers screened 10 preparation methods for graphene-on-glass and presented an optimized protocol for single-molecule and super-resolution imaging; they utilized DNA origami technology to detect graphene quality, showing discrepancies between conventional techniques such as light microscopy, atomic force microscopy, and Raman spectroscopy for graphene characterization.
Article
Chemistry, Physical
Stephan Benke, Andrea Holla, Bengt Wunderlich, Andrea Soranno, Daniel Nettels, Benjamin Schuler
Summary: This study combines three-color single-molecule FRET with rapid microfluidic mixing to investigate protein conformational changes with extended kinetics and reveals a previously undetected intermediate. By employing this approach, complex biomolecular processes can be probed with high time resolution.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Sabera Millan, Behera Susrisweta, Harekrushna Sahoo
Summary: Nanoparticles can enter the body and interact with proteins, leading to changes in their structure and function. The impact of niobium pentoxide nanoparticles on blood proteins was studied using spectroscopic methods. The results showed that the fluorescence intensity of the proteins decreased upon interaction with the nanoparticles, and the nature of the quenching was further investigated. The study also looked at the structural perturbations of the proteins, the effect of temperature on protein stability, and the impact of the nanoparticles on protein functionality.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Multidisciplinary Sciences
Stefan Niekamp, Nico Stuurman, Nan Zhang, Ronald D. Vale
Summary: The study reveals that during motility, the motor protein dynein exhibits variability in the movement between its domains, with the AAA ring and MTBDs not always stepping simultaneously and taking differently sized steps. This variability results in a large number of unexpected conformational states of dynein during motility. The flexibility between major dynein domains is found to be critical for dynein motility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Pancheng Zhu, Vasileios A. Papadimitriou, Jeanne E. van Dongen, Julia Cordeiro, Yannick Neeleman, Albert Santoso, Shuyi Chen, Jan C. T. Eijkel, Hanmin Peng, Loes I. Segerink, Alina Y. Rwei
Summary: Here, we introduce an aptamer-based biosensor for real-time optical detection of endotoxin. The sensor utilizes the scattering of gold nanoparticles (AuNPs) coupled to a gold nanofilm, which is controlled by the conformational changes of an endotoxin-specific aptamer. It can be used in ensemble mode with a microspectrometer or single-particle mode with a color camera, achieving high sensitivity and resolution. This platform has the potential to enhance endotoxin detection and ensure the safety of medical, food, and pharmaceutical products.
Article
Chemistry, Physical
Eva Clemente, Marta Martinez-Moro, Duong N. Trinh, Mahmoud G. Soliman, Daniel I. R. Spencer, Richard A. Gardner, Maximilianos Kotsias, Ana Sanchez Iglesias, Sergio Moya, Marco P. Monopoli
Summary: This study characterized the biomolecular corona of gold nanoparticles in blood plasma and revealed that the protein composition of the corona varies with different plasma concentrations. The study also demonstrated that the glycans in the corona can interact with lectins with known affinity, suggesting their potential role in modulating nanoparticle biological processes.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Review
Nanoscience & Nanotechnology
Hongliang Chen, Chuancheng Jia, Xin Zhu, Chen Yang, Xuefeng Guo, J. Fraser Stoddart
Summary: Developing new materials is a long-standing goal in various fields, and understanding single-molecule reactions can deepen our understanding of chemical reactions and provide new frameworks in materials science. This review focuses on state-of-the-art chemical reactions in single-molecule junctions and highlights the advantages of real-time testbeds for studying reaction dynamics, intermediates, transition states, and solvent effects. The behavior of single-molecule reactions is compared with reactions in ensemble states, and the potential of leveraging single-molecule catalysis for large-scale materials production is explored.
NATURE REVIEWS MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Alexandre Chappard, Craig Leighton, Rebecca S. S. Saleeb, Kiani Jeacock, Sarah R. R. Ball, Katie Morris, Owen Kantelberg, Ji-Eun Lee, Elsa Zacco, Annalisa Pastore, Margaret Sunde, David J. J. Clarke, Patrick Downey, Tilo Kunath, Mathew H. H. Horrocks
Summary: Protein misfolding and aggregation are common features of neurogenerative disorders. Single-molecule techniques have allowed the characterization of these protein aggregates, previously inaccessible with traditional methods. In this study, we employed a high affinity antibody labeled with orthogonal fluorophores, along with fast-flow microfluidics and single-molecule confocal microscopy, to specifically detect alpha-synuclein in biologically relevant samples.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Eduardo M. Bruch, Melissa T. Warner, Sebastien Thomine, Leandro C. Tabares, Sun Un
JOURNAL OF PHYSICAL CHEMISTRY B
(2015)
Article
Biochemistry & Molecular Biology
E. M. Bruch, S. Thomine, L. C. Tabares, S. Un
Article
Biochemistry & Molecular Biology
E. M. Bruch, A. de Groot, S. Un, L. C. Tabares
Article
Chemistry, Physical
H. Y. Vincent Ching, Paul Demay-Drouhard, Helene C. Bertrand, Clotilde Policar, Leandro C. Tabares, Sun Un
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2015)
Article
Chemistry, Multidisciplinary
Florencia C. Mascali, H. Y. Vincent Ching, Rodolfo M. Rasia, Sun Un, Leandro C. Tabares
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Chemistry, Physical
Paul Demay-Drouhard, H. Y. Vincent Ching, Dmitry Akhmetzyanov, Regis Guillot, Leandro C. Tabares, Helene C. Bertrand, Clotilde Policar
Article
Chemistry, Physical
H. Y. Vincent Ching, Florencia C. Mascali, Helene C. Bertrand, Eduardo M. Bruch, Paul Demay-Drouhard, Rodolfo M. Rasia, Clotilde Policar, Leandro C. Tabares, Sun Un
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2016)
Article
Chemistry, Physical
D. Akhmetzyanov, H. Y. V. Ching, V. Denysenkov, P. Demay-Drouhard, H. C. Bertrand, L. C. Tabares, C. Policar, T. F. Prisner, S. Un
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2016)
Article
Chemistry, Inorganic & Nuclear
Maria Strianese, Gottfried J. Palm, David Kohlhause, Lionel A. Ndamba, Leandro C. Tabares, Claudio Pellecchia
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Anna Barwinska-Sendra, Yuritzi M. Garcia, Kacper M. Sendra, Arnaud Basle, Eilidh S. Mackenzie, Emma Tarrant, Patrick Card, Leandro C. Tabares, Cedric Bicep, Sun Un, Thomas E. Kehl-Fie, Kevin J. Waldron
NATURE COMMUNICATIONS
(2020)
Article
Biophysics
Rosalin Bonetta, Gary J. Hunter, Chi H. Trinh, Tomasz Borowski, Anthony G. Fenech, Maria Kulp, Leandro C. Tabares, Sun Un, Therese Hunter
Summary: A mutant of C. elegans manganese superoxide dismutase was generated using site-directed mutagenesis, which altered metal selectivity in the protein and promoted cellular proliferation in K562 cells when added exogenously. This mutation resulted in changes to the active-site coordination from trigonal bipyramidal to octahedral geometry.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Chemistry, Physical
Paul Demay-Drouhard, H. Y. Vincent Ching, Christophe Decroos, Regis Guillot, Yun Li, Leandro C. Tabares, Clotilde Policar, Helene C. Bertrand, Sun Un
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2020)
Meeting Abstract
Biology
A. Bortolotti, L. C. Tabares, M. Temperini, N. Cortez
Meeting Abstract
Biochemistry & Molecular Biology
L. C. Tabares, E. M. Bruch, S. Un, A. de Groot
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2014)
Meeting Abstract
Biochemistry & Molecular Biology
E. M. Bruch, L. C. Tabares, S. Un
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2014)
