Article
Biochemistry & Molecular Biology
Takahito Suzuki, Satoshi Sakai, Kosuke Ota, Mika Yoshida, Chiharu Uchida, Hiroyuki Niida, Takafumi Suda, Masatoshi Kitagawa, Tatsuya Ohhata
Summary: A novel mechanism for regulation in lung adenocarcinoma, the LINC00173-SNAIL-FHIT axis, is uncovered in this study. LINC00173 positively regulates FHIT gene expression by binding to SNAIL and inhibiting its function, which may be a key mechanism for carcinogenesis and progression in lung adenocarcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Jenny T. Mao, Bingye Xue, Qing-Yi Lu, Laurie Lundmark, Windie Burns, Jieping Yang, Ru-Po Lee, Joseph Glass, Clifford Qualls, Larry Massie
Summary: This study aims to investigate the antineoplastic effects of Grape seed procyanidin extract (GSE) and milk thistle silymarin extract (MTE) against lung cancer. The results showed that the combination of GSE and MTE could enhance their anticancer effects without toxicity. Furthermore, GSE and MTE were found to upregulate miR-663a and FHIT genes in lung cancer cell lines, further enhancing their anticancer properties.
Article
Medicine, General & Internal
Hae-Jung Chae, Jong Bae Seo, Sung-Hak Kim, Young-Jun Jeon, Sung-Suk Suh
Summary: The study shows that Fhit gene overexpression induces Lin28B protein expression, which suppresses Let-7 miRNA and leads to increased miRNA redundancy in the miR-17/92 cluster. Additionally, ELF-1 and STAT1-dependent mechanisms are responsible for inducing Let-7 expression in Fhit-expressing cancer cells.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Cell Biology
Sonnhild Mittag, Franziska Wetzel, Sebastian Y. Mueller, Otmar Huber
Summary: In previous studies, Fhit and Nit1 have been identified as tumor suppressor proteins and interaction partners of beta-catenin, both acting as repressors of the canonical Wnt pathway. The Rosetta Stone hypothesis states that if proteins are expressed as fusion proteins in one organism and as single proteins in others, they should physically interact and share signaling functions. We tested this hypothesis and found a direct association between Nit1 and Fhit, as well as other common interaction partners Hsp60 and Ubc9.
Article
Multidisciplinary Sciences
Lena Krueger, Christoph J. Albrecht, Hannah K. Schammann, Florian M. Stumpf, Marie L. Niedermeier, Yizhi Yuan, Katrin Stuber, Josua Wimmer, Florian Stengel, Martin Scheffner, Andreas Marx
Summary: The nucleotides diadenosine triphosphate (Ap(3)A) and diadenosine tetraphosphate (Ap(4)A) act as alarmones triggering stress adaptive processes in cells. A study has developed photoaffinity-labeling probes to identify proteins interacting with Ap(3)A and Ap(4)A in human cell lysates, revealing their involvement in various cellular processes. This research provides valuable insights into the functions of these nucleotides and offers useful tools for further characterization of their regulatory mechanisms in cells.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Mi-Jeong Kim, Yoon Min, Soo-Kyung Jeong, Juhee Son, Ji Young Kim, Ji Su Lee, Duk-Hwan Kim, Joo Sang Lee, Eunyoung Chun, Ki-Young Lee
Summary: The study reveals that USP15 is significantly downregulated in lung cancer patients, and its knockout leads to increased migration, invasion, and autophagy induction in lung cancer cells. USP15 interacts with BECN1 and deubiquitinates it, thereby attenuating autophagy induction. Additionally, the expression of USP15 is associated with the upregulation of certain oncogenes and downregulation of tumor suppressor genes in lung cancer patients.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Xianglian Zhou, Yuting Pan, Yue Wang, Bojun Wang, Yu Yan, Yi Qu, Xisong Ke
Summary: In this study, FHIT protein was identified as a direct binding protein of STS, inhibiting its Ap3A hydrolase activity through competition for the substrate-binding site. Additionally, TSA, TanI, and CST showed similar inhibitory activity. Depletion of FHIT blocked TSA's pro-apoptotic function in colorectal cancer cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Zhangjie Wang, Na Shen, Ziao Wang, Lei Yu, Song Yang, Yang Wang, Yu Liu, Gaohua Han, Qi Zhang
Summary: This study reveals that TRIM3 functions as a tumor suppressor in non-small cell lung cancer (NSCLC) by promoting cell death and inhibiting tumorigenesis. TRIM3 directly interacts with SLC7A11/xCT, leading to its degradation and inhibition of NSCLC development. Low TRIM3 expression is associated with a worse prognosis in NSCLC.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemical Research Methods
Chen Hong, Robin Thiele, Lars Feuerbach
Summary: Analyzing focal copy number variations (CNVs) is crucial for cancer research as it helps identify driver genes. We have developed the GenomeTornadoPlot software package that provides visualizations of CNV types, locations, and lengths, and allows for gene-to-gene comparisons to identify co-mutation patterns or driver-passenger gene hierarchies.
Article
Cell Biology
Jianye Xu, Jian Zhang, Zongpu Zhang, Zijie Gao, Yanhua Qi, Wei Qiu, Ziwen Pan, Qindong Guo, Boyan Li, Shulin Zhao, Xiaofan Guo, Mingyu Qian, Zihang Chen, Shaobo Wang, Xiao Gao, Shouji Zhang, Huizhi Wang, Xing Guo, Ping Zhang, Rongrong Zhao, Hao Xue, Gang Li
Summary: The study reveals that glioma-derived exosomes may promote glioma proliferation and migration by inducing autophagy and M2-like macrophage polarization, leading to the formation of an immunosuppressive microenvironment. IL-6 and miR-155-3p could be novel biomarkers for diagnosing glioma, while treatments targeting autophagy and the STAT3 pathway may contribute to antitumor immunotherapy.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Kailing Zhou, Yu Sun, Dan Dong, Chenghai Zhao, Wei Wang
Summary: EMP3 acts as a potential tumor suppressor in breast cancer by inhibiting cell cycle progression, DNA repair, and stem-like properties, leading to enhanced sensitivity of breast cancer cells to DNA-damaging agents.
CELL DEATH & DISEASE
(2021)
Editorial Material
Hematology
Ian J. Majewski
Summary: The study investigates how loss of the tumor suppressor gene Cux1 modulates DNA repair activity in the hematopoietic compartment and contributes to the pathogenesis of therapy-related myeloid neoplasms.
Article
Biochemistry & Molecular Biology
Yang Li, Min Liu, Shuting Yang, Ashley M. Fuller, T. S. Karin Eisinger-Mathason, Shuying Yang
Summary: This study identifies RGS12 as a novel tumor suppressor in osteosarcoma through inhibiting the YAP-TEAD1-Ezrin signaling pathway and provides evidence that targeting RGS12 may be a therapeutic strategy for osteosarcoma.
Review
Biochemistry & Molecular Biology
Manlio Tolomeo, Antonio Cascio
Summary: STAT3 is a complex regulator of transcription, with constitutive activation reported in many tumors but less frequent somatic mutations in cancer. Oncogenic targets of STAT3 have been identified, and inhibitors have been developed for cancer prevention and treatment. However, data suggest that STAT3 functions are multifaceted and its cellular role is determined by various signals and factors, making targeted therapies tailored to specific tumor types important.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Nazma Malik, Bibiana I. Ferreira, Pablo E. Hollstein, Stephanie D. Curtis, Elijah Trefts, Sammy Weiser Novak, Jingting Yu, Rebecca Gilson, Kristina Hellberg, Lingjing Fang, Arlo Sheridan, Nasun Hah, Gerald S. Shadel, Uri Manor, Reuben J. Shaw
Summary: Cells respond to mitochondrial toxins by activating AMPK, which leads to acute metabolic changes and transcriptional effects. AMPK phosphorylates FNIP1, suppressing the function of the FLCN-FNIP1 complex and enabling nuclear translocation of TFEB. This ultimately leads to lysosomal and mitochondrial biogenesis.
Article
Pathology
Ankur K. Patel, Xueliang Pan, Diana M. Vila, Wendy L. Frankel, Wei Chen, Kyle A. Perry, Robert E. Merritt, Desmond M. D'Souza, Evan J. Wuthrick, Terence M. Williams
Summary: The study found that the presence of PNI in EAC patients during surgery is significantly associated with worse LRFS and DFS, indicating that considering adjuvant therapy in PNI patients may be beneficial.
JOURNAL OF CLINICAL PATHOLOGY
(2021)
Article
Pathology
Rongqin Ren, Somashekar G. Krishna, Wei Chen, Wendy L. Frankel, Rulong Shen, Weiqiang Zhao, Matthew R. Avenarius, Jason Garee, Sean Caruthers, Dan Jones
Summary: Molecular testing for KRAS/GNAS mutations can improve the accuracy of detecting mucinous pancreatic cystic lesions. Using next-generation sequencing, the status of PCL-associated mutations can be assessed to enhance diagnostic understanding.
Article
Oncology
Heather Hampel, Rachel Pearlman, Albert de la Chapelle, Colin C. Pritchard, Weiqiang Zhao, Dan Jones, Ahmet Yilmaz, Wei Chen, Wendy L. Frankel, Adrian A. Suarez, Casey Cosgrove, Floor Backes, Larry Copeland, Jeffrey Fowler, David O'Malley, Ritu Salani, Joseph P. McElroy, Peter P. Stanich, Paul Goodfellow, David E. Cohn
Summary: The study revealed that double somatic MMR gene PV are as common as Lynch syndrome among endometrial cancer patients. Paired tumor and germline testing for patients with non-methylated dMMR tumor may be the most efficient approach for Lynch syndrome screening.
GYNECOLOGIC ONCOLOGY
(2021)
Article
Cell Biology
Amy Lord, Gina Brown, Muti Abulafi, Adrian Bateman, Wendy Frankel, Robert Goldin, Purva Gopal, Richard Kirsch, Maurice B. Loughrey, Bruno Maerkl, Brendan Moran, Giacomo Puppa, Shahnawaz Rasheed, Yoshifumi Shimada, Petur Snaebjornsson, Magali Svrcek, Kay Washington, Nicholas West, Newton Wong, Iris Nagtegaal
Summary: Tumour deposits (TDs) are an important prognostic marker in colorectal cancer, but their classification and inclusion in staging are controversial. Expert consensus suggests a need for a clearer, more reproducible definition of TDs, and the recommendation to record the number of TDs and not exclude nodules with evidence of origin.
Article
Pathology
Jennifer Vazzano, Wendy L. Frankel, Adam R. Wolfe, Terence M. Williams, Wei Chen
Summary: This study analyzed PDAC cases and found significant histologic differences between NAT and non-NAT cases, with blue/grey fibrosis being a useful clue to suggest the possibility of adjacent tumor in the majority of PDAC cases. Interestingly, while neither grading scheme correlated with overall survival, the MDA score was significantly correlated with time to primary tumor recurrence and time to distant recurrence.
Article
Medical Laboratory Technology
Jesus A. Chavez, Wei Chen, C. Eric Freitag, Wendy L. Frankel
Summary: This study evaluated the use and accuracy of frozen section in pancreatic resections and how diagnosis impacts surgical procedure. The results showed that frozen section remains an important diagnostic tool in pancreatectomy, with high accuracy and occasional deferrals.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Giovanni M. Lujan, Johanna Savage, Arwa Shana'ah, Martha Yearsley, Diana Thomas, Patricia Allenby, Jose Otero, Abberly Lott Limbach, Xiaoyan Cui, Rachel T. Scarl, Tanner Hardy, Jesse Sheldon, Jose A. Plaza, Bonnie Whitaker, Wendy Frankel, Anil V. Parwani, Zaibo Li
Summary: The pandemic acted as a catalyst for pathologists to adopt a digital workflow into their daily practice, realizing the logistic and technical advantages of such tools.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2021)
Article
Oncology
Lanchun Lu, Zhilin Hu, Wendy Frankel, Rulong Shen, Wei Chen, Xueliang Pan, John C. Grecula, Mark P. Bloomston, Mary E. Dillhoff
Summary: The novel technology integrates high-resolution three-dimensional endoscopic optical coherence tomography (Endo-OCT) for early-stage pancreatic cancer detection and high dose rate brachytherapy (HDR) for treatment under Endo-OCT image guidance. It offers a compact, portable, easy-to-operate, and low-cost system with dual functions of diagnosis and treatment to reduce potential complications from surgery and high operation costs.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Karyn A. Goodman, Fang-Shu Ou, Nathan C. Hall, Tanios Bekaii-Saab, Briant Fruth, Erin Twohy, Michael O. Meyers, Daniel J. Boffa, Kisha Mitchell, Wendy L. Frankel, Donna Niedzwiecki, Anne Noonan, Yelena Y. Janjigian, Paul J. Thurmes, Alan P. Venook, Jeffrey A. Meyerhardt, Eileen M. O'Reilly, David H. Ilson
Summary: The study evaluated the use of early assessment of chemotherapy responsiveness by PET imaging to tailor therapy for patients with esophageal and esophagogastric junction adenocarcinoma. Results showed improved pathologic complete response rates in PET nonresponders, with PET responders to FOLFOX achieving a promising 5-year overall survival of 53%.
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Oncology
Debanjan Chakroborty, Sandeep Goswami, Hao Fan, Wendy L. Frankel, Sujit Basu, Chandrani Sarkar
Summary: This study found that NPY and Y2R are overexpressed in colon adenocarcinoma. Treatment with Y2R antagonists can inhibit angiogenesis and tumor growth. NPY regulates the angiogenic potential of colonic endothelial cells through Y2R and activates the ERK/MAPK signaling pathway.
BRITISH JOURNAL OF CANCER
(2022)
Article
Oncology
Jennifer Vazzano, Jewel Tomlinson, Peter P. Stanich, Rachel Pearlman, Matthew F. Kalady, Wei Chen, Heather Hampel, Wendy L. Frankel
Summary: This study examined the utilization of universal tumor screening (UTS) on colorectal cancer (CRC) patients and evaluated the impact of genetic counseling and testing on surgical decisions. The results showed that UTS performed on biopsies provided valuable information for genetic counseling and testing, leading to informed surgical decisions.
Article
Oncology
Matthew H. G. Katz, Qian Shi, Jeff Meyers, Joseph M. Herman, Michael Chuong, Brian M. Wolpin, Syed Ahmad, Robert Marsh, Larry Schwartz, Spencer Behr, Wendy L. Frankel, Eric Collisson, James Leenstra, Terence M. Williams, Gina Vaccaro, Alan Venook, Jeffrey A. Meyerhardt, Eileen M. O'Reilly
Summary: This randomized clinical trial found that treatment with neoadjuvant modified FOLFIRINOX alone was associated with favorable overall survival (OS) in patients with borderline resectable pancreatic ductal adenocarcinoma (PDAC) compared with treatment with modified FOLFIRINOX plus hypofractionated radiotherapy. Therefore, modified FOLFIRINOX represents a reference regimen in this setting.
Article
Pathology
C. Eric Freitag, Wei Chen, Rachel Pearlman, Heather Hampel, Peter P. Stanich, Casey M. Cosgrove, Eric Q. Konnick, Colin C. Pritchard, Wendy L. Frankel
Summary: Mismatch repair (MMR) protein-deficient colonic crypts and endometrial glands (dMMR crypts and glands) are unique markers of underlying Lynch syndrome (LS) and are rarer in double somatic (DS) MMR-mutated cases. A retrospective analysis of colonic resection specimens and endometrial specimens revealed that dMMR crypts were more frequent in LS cases compared to DS cases, while dMMR glands were also more common in LS cases than in DS cases. The presence of dMMR crypts and glands is highly associated with LS.
Article
Pathology
Denise Gamble, Wendy L. Frankel, Martha M. Yearsley, Wei Chen
Summary: Endoscopic biopsies of the ampulla of Vater are challenging due to sampling limitations and the complexity of local anatomy. A retrospective review of 318 biopsy specimens from 252 patients found that a significant proportion of cases were diagnosed as adenoma or adenocarcinoma. Follow-up specimens showed discordant results in 38.9% of cases, with major discrepancies observed in 22.9% of cases.
ANNALS OF DIAGNOSTIC PATHOLOGY
(2023)
Article
Oncology
Rachel Pearlman, Wendy L. Frankel, Benjamin J. Swanson, Dan Jones, Weiqiang Zhao, Ahmet Yilmaz, Kristin Miller, Jason Bacher, Christopher Bigley, Lori Nelsen, Paul J. Goodfellow, Richard M. Goldberg, Electra Paskett, Peter G. Shields, Jo L. Freudenheim, Peter P. Stanich, Ilene Lattimer, Mark Arnold, Thomas W. Prior, Mitchell Haut, Matthew F. Kalady, Brandie Heald, Ian Paquette, David J. Draper, Joanna M. Brell, Sameer Mahesh, Kisa Weeman, Shyamal Bastola, Jeffrey Zangmeister, Aruna Gowda, Filix Kencana, Albert Malcolm, Yinong Liu, Sharon Cole, Charles Bane, Chaoyang Li, Esther Rehmus, Colin C. Pritchard, Brian H. Shirts, Angela Jacobson, Shelly A. Cummings, Albert de la Chapelle, Heather Hampel
Summary: The study found that 7.1% of individuals with CRC have PGVs, and UTS alone is insufficient for identifying a large proportion of CRC patients with hereditary syndromes, including some with LS. Pan-cancer MGPT for all patients with CRC may be a better screening method.
JCO PRECISION ONCOLOGY
(2021)