Article
Cell Biology
Katharina Joechle, Huda Jumaa, Kerstin Thriene, Claus Hellerbrand, Birte Kulemann, Stefan Fichtner-Feigl, Sven A. Lang, Jessica Guenzle
Summary: The study showed that OSI-027, a dual kinase inhibitor targeting mTOR complexes, can significantly impact the migratory and metastatic capacity of cholangiocarcinoma cells by reducing cell mobility and migration, decreasing colony-forming ability, and downregulating MMP2 and MMP9 expression. Additionally, OSI-027 impaired survival and anti-apoptotic signaling as determined by AKT and MAPK blotting, suggesting that dual targeting of mTORC1/2 may be a viable option for anti-neoplastic therapy in CCA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jessica Guenzle, Harue Akasaka, Katharina Joechle, Wilfried Reichardt, Aina Venkatasamy, Jens Hoeppner, Claus Hellerbrand, Stefan Fichtner-Feigl, Sven A. Lang
Summary: This study demonstrates the significant reduction in migration, invasion, and proliferation of melanoma cells upon specific inhibition of mTORC2 using the novel inhibitor JR-AB2-011, leading to cell death through non-apoptotic pathways. In a syngeneic murine metastasis model, therapy with JR-AB2-011 showed a remarkable decrease in liver metastasis, highlighting the potential of pharmacological blockade of mTORC2 as a novel anti-cancer approach for melanoma liver metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anne S. Boewe, Silke Wemmert, Philipp Kulas, Bernhard Schick, Claudia Goetz, Selina Wrublewsky, Mathias Montenarh, Michael D. Menger, Matthias W. Laschke, Emmanuel Ampofo
Summary: The inhibition of CK2 may represent a promising therapeutic approach for the treatment of NG2-expressing juvenile angiofibroma, as it can significantly reduce NG2 expression and suppress the proliferation and migration of JA cells.
Article
Immunology
Lei Chu, Chenhui Li, Yongxing Li, Qiuya Yu, Huansha Yu, Chunhui Li, Wei Meng, Juanjuan Zhu, Quanyi Wang, Chen Wang, Shufang Cui
Summary: The study found that perillaldehyde (PAH) derived from Perilla frutescens can suppress cytosolic-DNA-induced innate immune responses by inhibiting cGAS activity, potentially benefiting the treatment of autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Mengna Zhang, Lingxian Zhang, Ajun Geng, Xiao Li, Yu Zhou, Liming Xu, Yi Arial Zeng, Jinpeng Li, Cheguo Cai
Summary: In this study, the researchers investigated the function of cyclin-dependent kinase 14 (CDK14) in mammary development and breast cancer progression. The results showed that CDK14 is expressed in mammary basal cells and elevated in triple negative breast cancer (TNBC). CDK14 knockdown or inhibition was found to suppress mammary regeneration and the progression of TNBC.
Article
Biochemistry & Molecular Biology
Alexandra A. Tsitrina, Igor Krasylov, Dmitry Maltsev, Irina N. Andreichenko, Viktoria S. Moskvina, Dmitry N. Ivankov, Elena Bulgakova, Mikhail Nesterchuk, Vera Shashkovskaya, Nataliya O. Dashenkova, Vladimir P. Khilya, Arsen Mikaelyan, Yuri Kotelevtsev
Summary: Elevated levels of hyaluronic acid (HA) are used as a disease marker in liver pathology and other inflammatory disorders. Inhibitors like coumarin 4-methylumbelliferone (4MU) and newly synthesized coumarin compounds, along with commercial chitin synthesis inhibitors, have shown potential in inhibiting HA production in cell culture assays. Specific inhibitors of hyaluronan synthases (HAS) are needed for more effective treatment options.
Article
Medicine, Research & Experimental
Junlong Yang, Liu Wang, Zhongjie Zhang, Qing Sun, Yuan Zhang
Summary: The downregulation of HAS-2 activates the RhoA/ROCK pathway, causing abnormal morphology and decreased protein expression in chondrocyte cytoskeleton, leading to chondrocyte apoptosis and cartilage degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2023)
Article
Endocrinology & Metabolism
Nadine Nagy, Gernot Kaber, Michael J. Kratochvil, Hedwich F. Kuipers, Shannon M. Ruppert, Koshika Yadava, Jason Yang, Sarah C. Heilshorn, S. Alice Long, Alberto Pugliese, Paul L. Bollyky
Summary: IL-2 injections are a promising therapy for autoimmune type 1 diabetes, but their short half-life in vivo limits effective tissue exposure. This study investigated the use of an injectable hydrogel containing heparin to promote prolonged IL-2 release, which was found to enhance IL-2 activity and prevent autoimmune diabetes in a mouse model. The hydrogel delivery system may reduce the frequency of injections needed for IL-2 treatment protocols.
Article
Oncology
Lisa Bierbaumer, Anna M. Katschnig, Branka Radic-Sarikas, Maximilian O. Kauer, Jeffrey A. Petro, Sandra Hoegler, Elisabeth Gurnhofer, Gloria Pedot, Beat W. Schafer, Raphaela Schwentner, Karin Muehlbacher, Florian Kromp, Dave N. T. Aryee, Lukas Kenner, Aykut Uren, Heinrich Kovar
Summary: Ewing sarcoma cells exhibit high plasticity and can switch between different functional states under the regulation of EWS-FLI1. Inhibiting the YAP/TAZ pathway has been shown to suppress migration and metastasis of Ewing sarcoma cells.
Article
Respiratory System
Cheng Xiang Foo, Stacey Bartlett, Keng Yih Chew, Minh Dao Ngo, Helle Bielefeldt-Ohmann, Buddhika Jayakody Arachchige, Benjamin Matthews, Sarah Reed, Ran Wang, Christian Smith, Matthew J. Sweet, Lucy Burr, Kavita Bisht, Svetlana Shatunova, Jane E. Sinclair, Rhys Parry, Yuanhao Yang, Jean-Pierre Levesque, Alexander Khromykh, Mette Marie Rosenkilde, Kirsty R. Short, Katharina Ronacher
Summary: This study identifies oxysterols as drivers of immune cell infiltration and inflammation in the lungs via GPR183, providing new drug targets for the treatment of severe viral respiratory infections.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Shaomin Zhi, Jun Li, Xiao Kong, Xuemei Xie, Qiangli Zhang, Guoxiang Fang
Summary: IGF2BP2 plays a crucial role in wound healing by regulating proliferation, migration, and angiogenesis of keratinocytes. Knockdown of IGF2BP2 inhibits these processes in keratinocytes by decreasing the stability of HPSE.
Article
Cell Biology
Yumeng Sheng, Manlin Cao, Yiwen Liu, Yiqing He, Guoliang Zhang, Yan Du, Feng Gao, Cuixia Yang
Summary: The study found that the level of HAS2 is positively correlated with the malignant phenotype of breast cancer cells, with its overexpression promoting cell invasion and migration abilities, and influencing the expression of related proteins. HAS2 can also enhance invadopodia formation, driving the transition to an invasive phenotype.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Nadine Nagy, Gernot Kaber, Payton L. Marshall, Aviv Hargil, Hedwich F. Kuipers, Heather D. Ishak, Marika Bogdani, Rebecca L. Hull, Maria Grandoch, Jens W. Fischer, Tracey L. Mclaughlin, Thomas N. Wight, Paul L. Bollyky, Vivekananda G. Sunkari
Summary: This study identifies a novel role for the inflammatory extracellular matrix polymer hyaluronan (HA) in the pathogenesis of type 2 diabetes. The inhibition of HA synthesis or deletion of the main HA receptor CD44 can preserve glycemic control and insulin concentrations in diabetic mice, indicating the potential of HA as a target for ameliorating beta-cell loss in type 2 diabetes.
Article
Multidisciplinary Sciences
Emerson Soares Veloso, Barbara Andrade de Carvalho, Felipe Henrique de Souza Silva, Thais Salviana Ribeiro, Bruna Mendes Lima, Camila Pereira Almeida, Vitor Henrique Soares Romao da Silva, Sara Aparecida Rocha, Marina Rios de Araujo Campos, Helen Lima Del Puerto, Enio Ferreira
Summary: Metformin can inhibit EMT and reduce the metastatic potential of melanoma cells, suggesting its potential role in the prevention and treatment of melanoma.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Maria Victoria Ruiz-Perez, Lourdes Sainero-Alcolado, Ganna Oliynyk, Isabell Matuschek, Nicola Balboni, S. J. Kumari A. Ubhayasekera, Marteinn Thor Snaebjornsson, Kamil Makowski, Kristina Aaltonen, Daniel Bexell, Dolors Serra, Roland Nilsson, Jonas Bergquist, Almut Schulze, Marie Arsenian-Henriksson
Summary: Metabolic pathways, especially lipid metabolism, are rewired in tumors to support their growth and adaptation to stressful environments. In neuroblastoma, inhibition of fatty acid synthesis led to increased neural differentiation and reduced tumor burden independently of MYCN-status, showing promise as a pharmacological intervention strategy.
Article
Dermatology
Danielle M. DeBruler, Molly E. Baumann, Jacob Zbinden, Britani N. Blackstone, John Kevin Bailey, Dorothy M. Supp, Heather M. Powell
ADVANCES IN WOUND CARE
(2020)
Article
Critical Care Medicine
Molly E. Baumann, Danielle M. DeBruler, Britani N. Blackstone, Rebecca A. Coffey, Steven T. Boyce, Dorothy M. Supp, J. Kevin Bailey, Heather M. Powell
Summary: Assessing scar properties quantitatively with non-invasive instruments is more reliable and efficient than using scar scales, with color and biomechanics measurements taking less than 90 s per measurement and providing high sensitivity over time or treatment.
Article
Cell & Tissue Engineering
Megan M. Malara, Britani N. Blackstone, Molly E. Baumann, J. Kevin Bailey, Dorothy M. Supp, Heather M. Powell
TISSUE ENGINEERING PART A
(2020)
Letter
Critical Care Medicine
Heather M. Powell, Dorothy M. Supp, J. Kevin Bailey, Molly E. Baumann
Article
Critical Care Medicine
Angela L. F. Gibson, Bonnie C. Carney, Leila Cuttle, Christine J. Andrews, Christine J. Kowalczewski, Aiping Liu, Heather M. Powell, Randolph Stone, Dorothy M. Supp, Adam J. Singer, Jeffrey W. Shupp, Lily Stalter, Lauren T. Moffatt
Summary: Deep partial thickness burns are commonly seen in clinical practice but are hard to diagnose. Accurate animal models are crucial for developing methods to assess burn depth and therapies to treat these burns. Inconsistencies in evaluation approaches among different laboratories were found, leading to the development of a scoring rubric that improved reliability in assessing burn injury depth in pig models.
JOURNAL OF BURN CARE & RESEARCH
(2021)
Article
Cell & Tissue Engineering
Sonya Ruiz-Torres, Marion G. Brusadelli, David P. Witte, Kathryn A. Wikenheiser-Brokamp, Sharon Sauter, Adam S. Nelson, Mathieu Sertorio, Timothy M. Chlon, Adam Lane, Parinda A. Mehta, Kasiani C. Myers, Mary C. Bedard, Bidisha Pal, Dorothy M. Supp, Paul F. Lambert, Kakajan Komurov, Melinda Butsch Kovacic, Stella M. Davies, Susanne Wells
Summary: The study found that genetic mutations in Fanconi anemia (FA) patients can affect cell structure and function, accelerating the process of cancer development. This provides a new direction for exploring SCC prevention.
Article
Cell Biology
Jennifer M. Hahn, Kevin L. McFarland, Kelly A. Combs, Meridith C. Anness, Dorothy M. Supp
Summary: Keloids are fibroproliferative lesions resulting from abnormal wound healing, with different expression of HOX genes in keloid and normal skin cells potentially playing a role in keloid development. However, the differential expression of HOX genes may be due to positional identity differences between keloid and normal fibroblasts rather than body site-specific factors.
WOUND REPAIR AND REGENERATION
(2021)
Article
Multidisciplinary Sciences
Christian B. Bergmann, Bruce D. Hammock, Debin Wan, Falk Gogolla, Holly Goetzman, Charles C. Caldwell, Dorothy M. Supp
Summary: The study demonstrated that TPPU administration can decrease DHET levels post-burn, leading to reduced IL-6 concentrations. 14,15-DHET was found to modulate neutrophil functionality and mRNA expression, indicating its role in regulating inflammation in burn injury.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Alexander Bondoc, Kathryn Glaser, Kang Jin, Charissa Lake, Stefano Cairo, James Geller, Gregory Tiao, Bruce Aronow
Summary: This study utilized single cell RNA sequencing to analyze gene expression patterns in hepatoblastoma tumor, identifying a driver malignant cell cluster and proposing potential roles in treatment research.
COMMUNICATIONS BIOLOGY
(2021)
Article
Critical Care Medicine
David M. Burmeister, Dorothy M. Supp, Richard A. Clark, Edward E. Tredget, Heather M. Powell, Perenlei Enkhbaatar, Julia K. Bohannon, Leopoldo C. Cancio, David M. Hill, Rachel M. Nygaard
Summary: This article introduces the use of multiple animal species and approaches for modeling burn care and discusses the pros and cons of using small and large animal models for research. Researchers believe that using pigs as large animal models can better simulate human skin, while sheep models are suitable for studying inhalation injury. However, these models are expensive and technically demanding. Therefore, by improving small animal models, questions related to burns can be addressed.
JOURNAL OF BURN CARE & RESEARCH
(2022)
Review
Oncology
Emily J. Schepers, Kathryn Glaser, Harrison M. Zwolshen, Stephen J. Hartman, Alexander J. Bondoc
Summary: This article reviews the structure and function of GPC3 in liver cancer, specifically focusing on the posttranslational modifications (PTM) that regulate its tertiary and quaternary structures. The study proposes that GPC3's function in normal development can be influenced by extensive PTMs, and dysregulation of these processes can lead to disease. Understanding the regulatory impact of these modifications provides insights into GPC3's role in oncogenesis, epithelial-mesenchymal transition, and drug development.
Article
Cell Biology
Jennifer M. Hahn, Kelly A. Combs, Heather M. Powell, Dorothy M. Supp
Summary: Keloids are challenging to treat and have high recurrence rates. The genetic causes and molecular mechanisms of keloid formation are not fully understood. Dark-skinned individuals, who are at higher risk of keloids, may also have lower vitamin D levels, suggesting a potential role for vitamin D in keloid pathology. Studies have found reduced vitamin D receptor expression in keloid lesions, indicating a possible therapeutic role for vitamin D in suppressing keloid fibrosis.
WOUND REPAIR AND REGENERATION
(2023)
Article
Oncology
Kathryn Glaser, Emily J. Schepers, Harrison M. Zwolshen, Charissa M. Lake, Nikolai A. Timchenko, Rebekah A. Karns, Stefano Cairo, James I. Geller, Gregory M. Tiao, Alexander J. Bondoc
Summary: This study reveals that EZH2 promotes proliferation in HB tumor-derived cell lines through interaction with beta-catenin, suggesting a noncanonical mechanism contributing to HB pathogenesis. Further mechanistic studies are needed to explore potential downstream mechanisms and translational potential of EZH2 inhibitors for HB treatment.
PEDIATRIC BLOOD & CANCER
(2023)
Article
Biochemical Research Methods
Dorothy M. Supp, Jennifer M. Hahn, Kelly A. Combs, Kevin L. Mcfarland, Heather M. Powell
Summary: A method has been developed to isolate four types of primary cells from a single human skin sample without using murine feeder cells. These isolated cells contain stem cell populations and are capable of forming functional skin tissue.
Meeting Abstract
Cell Biology
Dorothy M. Supp, Jennifer M. Hahn, Kevin L. McFarland, Kelly A. Combs, Meridith Anness
WOUND REPAIR AND REGENERATION
(2021)